MD05G1156600.v1.1

Calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012876
Physical Location & Seq
Reverse (-)
28635783 .. 28636241
459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1156600.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGACAGGGCGGAGGTACGGCGGGTTTTCCAGATGTTTGATCAGAACGGGGACGGGCGGATCACGAAGAAGGAGCTGAGGGACTCGTTGGAGAAGCTGGGGATTTACATTCCGGACAAAGACTTGATCCAGATGATTGAAAAAATCGATGTCAATGGAGATGGGTTCGTGGACATTGATGAATTCGGGGCGTTGTACCAGACGATCATAGGCGAGAGGGACGAGGAGGAGGACATGAGGGAGGCGTTCAATGTGTTTGATCGGAACGGAGACGGATTCATCACGGTGGACGAGCTGCAGTCGGTTTTATCTTCCTTGGGGCTGAAGCAAGGAAGGACATTGGAGGATTGCAAGATCATGATAAAGAAGGTTGATGGTGATGGTGATGGGAGGGTGAATTTCAAGGAGTTTAAGCAGATGATGAAAGGTGGTGGGTTTGCTGCTTTAAGCTCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 ko04626 map03018 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

153

Amino Acids

17.23

Weight (kDa)

4.58

Isoelectric Point (pI)

23.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 5 - 32 1.3e-07 EF hand domain
EF-hand_7 PF13499 5 - 66 2.8e-14 EF-hand domain pair
EF-hand_6 PF13405 5 - 34 4.3e-08 EF-hand domain
EF-hand_5 PF13202 8 - 27 4.1e-07 EF hand
EF-hand_1 PF00036 42 - 67 4.2e-06 EF hand domain
EF-hand_7 PF13499 76 - 142 6e-17 EF-hand domain pair
EF-hand_6 PF13405 78 - 107 6.9e-10 EF-hand domain
EF-hand_1 PF00036 79 - 106 2.1e-08 EF hand domain
EF-hand_5 PF13202 80 - 103 4e-08 EF hand
EF-hand_8 PF13833 92 - 142 1.2e-09 EF-hand domain pair
EF-hand_1 PF00036 117 - 142 1.4e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07490 AT4G12860
fragaria_vesca FvH4_2g17620
malus_domestica MD05G1156600.v1.1 MD10G1150400.v1.1
prunus_persica Prupe.8G188800_v2.0.a1
pyrus_communis pycom05g14920 pycom10g12960
rosa_chinensis RchiOBHm_Chr6g0282601
rosa_laevigata RLG00000012882
rosa_multiflora Rmu_co8242345.1_g000002 Rmu_sc0000283.1_g000002 Rmu_sc0005697.1_g000002
rosa_roxburghii Rroxscaffold_7G00186230
rosa_rugosa Rorug06G0148000
rosa_samantha Rh6BG264300 Rh6CG263400 Rh6DG255400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 112
AciI CCGC 3 cut(s) 11, 22, 58
AclWI GGATC 2 cut(s) 68, 121
AcsI RAATTY 2 cut(s) 182, 397
AcuI CTGAAG 1 cut(s) 344
AfaI GTAC 2 cut(s) 18, 197
AgsI TTSAA 3 cut(s) 140, 250, 403
AluBI AGCT 4 cut(s) 76, 97, 295, 450
AluI AGCT 4 cut(s) 76, 97, 295, 450
Alw26I GTCTC 1 cut(s) 264
AlwI GGATC 2 cut(s) 68, 121
Aor13HI TCCGGA 1 cut(s) 112
ApeKI GCWGC 2 cut(s) 295, 440
ApoI RAATTY 2 cut(s) 182, 397
AsuHPI GGTGA 3 cut(s) 389, 395, 406
BbvCI CCTCAGC 1 cut(s) 77
BbvI GCAGC 2 cut(s) 282, 427
BccI CCATC 4 cut(s) 155, 368, 374, 380
BceAI ACGGC 1 cut(s) 35
BclI TGATCA 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 264
BfmI CTRYAG 1 cut(s) 296
BisI GCNGC 2 cut(s) 296, 441
BlsI GCNGC 2 cut(s) 297, 442
Bpu10I CCTNAGC 1 cut(s) 77
Bsa29I ATCGAT 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 112
BseAI TCCGGA 1 cut(s) 112
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 315
BseMII CTCAG 1 cut(s) 68
BseRI GAGGAG 2 cut(s) 239, 242
BseXI GCAGC 2 cut(s) 282, 427
BseYI CCCAGC 1 cut(s) 97
BshVI ATCGAT 1 cut(s) 147
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 3 cut(s) 65, 95, 233
BsmAI GTCTC 1 cut(s) 264
BsmBI CGTCTC 1 cut(s) 264
BsmFI GGGAC 3 cut(s) 65, 95, 233
Bsp13I TCCGGA 1 cut(s) 112
Bsp143I GATC 6 cut(s) 40, 60, 126, 204, 259, 354
BspACI CCGC 3 cut(s) 11, 22, 58
BspCNI CTCAG 1 cut(s) 69
BspDI ATCGAT 1 cut(s) 147
BspEI TCCGGA 1 cut(s) 112
BspHI TCATGA 1 cut(s) 357
BspMAI CTGCAG 1 cut(s) 300
BspPI GGATC 2 cut(s) 68, 121
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 6 cut(s) 40, 60, 126, 204, 259, 354
BssT1I CCWWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 286
BstDEI CTNAG 1 cut(s) 77
BstKTI GATC 6 cut(s) 43, 63, 129, 207, 262, 357
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 6 cut(s) 40, 60, 126, 204, 259, 354
BstSFI CTRYAG 1 cut(s) 296
BstV1I GCAGC 2 cut(s) 282, 427
Bsu15I ATCGAT 1 cut(s) 147
BsuTUI ATCGAT 1 cut(s) 147
CciI TCATGA 1 cut(s) 357
ClaI ATCGAT 1 cut(s) 147
Csp6I GTAC 2 cut(s) 17, 196
CviAII CATG 2 cut(s) 235, 358
CviJI RGCY 5 cut(s) 76, 97, 295, 322, 450
CviKI_1 RGCY 5 cut(s) 76, 97, 295, 322, 450
CviQI GTAC 2 cut(s) 17, 196
DdeI CTNAG 1 cut(s) 77
DpnI GATC 6 cut(s) 42, 62, 128, 206, 261, 356
DpnII GATC 6 cut(s) 40, 60, 126, 204, 259, 354
EciI GGCGGA 2 cut(s) 26, 73
Eco130I CCWWGG 1 cut(s) 315
Eco57I CTGAAG 1 cut(s) 344
EcoRI GAATTC 1 cut(s) 182
EcoT14I CCWWGG 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 264
FaeI CATG 2 cut(s) 238, 361
FaiI YATR 3 cut(s) 209, 236, 359
FaqI GGGAC 3 cut(s) 65, 95, 233
FatI CATG 2 cut(s) 234, 357
FauI CCCGC 1 cut(s) 15
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 2 cut(s) 296, 441
Fsp4HI GCNGC 2 cut(s) 296, 441
GluI GCNGC 2 cut(s) 296, 441
GsaI CCCAGC 1 cut(s) 101
HapII CCGG 1 cut(s) 113
Hin1II CATG 2 cut(s) 238, 361
HinfI GANTC 2 cut(s) 83, 276
HpaII CCGG 1 cut(s) 113
HphI GGTGA 3 cut(s) 389, 395, 406
Hpy166II GTNNAC 2 cut(s) 172, 289
Hpy188I TCNGA 2 cut(s) 45, 264
Hpy188III TCNNGA 5 cut(s) 31, 64, 113, 130, 358
Hpy8I GTNNAC 2 cut(s) 172, 289
HpyAV CCTTC 3 cut(s) 64, 327, 361
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 2 cut(s) 298, 351
HpyF3I CTNAG 1 cut(s) 77
Hsp92II CATG 2 cut(s) 238, 361
Kpn2I TCCGGA 1 cut(s) 112
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 6 cut(s) 40, 60, 126, 204, 259, 354
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 5 cut(s) 44, 83, 126, 143, 212
Lsp1109I GCAGC 2 cut(s) 282, 427
MalI GATC 6 cut(s) 42, 62, 128, 206, 261, 356
MboI GATC 6 cut(s) 40, 60, 126, 204, 259, 354
MboII GAAGA 2 cut(s) 79, 303
MluCI AATT 3 cut(s) 182, 397, 454
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 69
MroI TCCGGA 1 cut(s) 112
MseI TTAA 2 cut(s) 411, 446
MslI CAYNNNNRTG 1 cut(s) 284
MspI CCGG 1 cut(s) 113
NdeII GATC 6 cut(s) 40, 60, 126, 204, 259, 354
NlaIII CATG 2 cut(s) 238, 361
PagI TCATGA 1 cut(s) 357
PfeI GAWTC 1 cut(s) 276
PkrI GCNGC 2 cut(s) 297, 442
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PspFI CCCAGC 1 cut(s) 97
PstI CTGCAG 1 cut(s) 300
RsaI GTAC 2 cut(s) 18, 197
RsaNI GTAC 2 cut(s) 17, 196
RseI CAYNNNNRTG 1 cut(s) 284
SaqAI TTAA 2 cut(s) 411, 446
SatI GCNGC 2 cut(s) 296, 441
Sau3AI GATC 6 cut(s) 40, 60, 126, 204, 259, 354
SchI GAGTC 1 cut(s) 77
SetI ASST 7 cut(s) 18, 78, 99, 297, 372, 430, 452
SfcI CTRYAG 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 284
Sse9I AATT 3 cut(s) 182, 397, 454
SsiI CCGC 3 cut(s) 11, 22, 58
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 1 cut(s) 147
TasI AATT 3 cut(s) 182, 397, 454
TfiI GAWTC 1 cut(s) 276
Tru1I TTAA 2 cut(s) 411, 446
Tru9I TTAA 2 cut(s) 411, 446
TseI GCWGC 2 cut(s) 295, 440
TspDTI ATGAA 3 cut(s) 195, 268, 437
TspGWI ACGGA 2 cut(s) 282, 288
XapI RAATTY 2 cut(s) 182, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.