AT4G13260

L-lysine 6-monooxygenase (NADPH-requiring)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010803
Physical Location & Seq
Reverse (-)
7721487 .. 7724070
2584 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G13260.3

Sequence Viewer

Length: 1248 bp
ATGGAGTTTGTTACAGAAACGTTAGGCAAGAGAATCCATGATCCGTACGTGGAGGAAACTAGGTGCTTAATGATTCCCGGACCAATCATTGTCGGTTCCGGGCCGTCGGGACTGGCCACAGCGGCATGTTTAAAGTCGAGAGACATCCCTAGTTTGATTCTAGAACGTTCCACTTGCATAGCGTCACTATGGCAGCACAAAACATATGATCGTCTTCGACTTCATCTCCCTAAAGATTTCTGTGAGCTTCCATTGATGCCTTTTCCTTCAAGCTACCCTACTTACCCTACAAAGCAACAGTTCGTCCAATACCTTGAGTCTTACGCCGAACATTTTGACCTAAAGCCCGTTTTTAACCAGACCGTGGAGGAAGCCAAGTTCGATAGGCGGTGTGGGTTATGGAGGGTGAGGACAACCGGAGGGAAGAAGGATGAGACAATGGAGTATGTATCACGGTGGCTTGTTGTGGCGACCGGGGAGAATGCCGAGGAGGTGATGCCGGAGATTGATGGAATCCCGGATTTTGGTGGACCTATCCTCCACACAAGCTCCTATAAGAGCGGTGAAATATTTAGTGAGAAGAAGATTTTGGTTGTAGGATGTGGAAACTCCGGGATGGAAGTTTGTTTAGACCTTTGCAACTTCAATGCTCTTCCTTCTCTTGTGGTTCGTGACTCGGTACACGTATTACCTCAAGAAATGCTAGGTATATCGACTTTCGGGATATCCACGAGCCTGCTCAAGTGGTTTCCAGTGCACGTGGTGGACCGGTTCTTGTTACGTATGTCTCGGTTGGTTCTTGGTGACACGGATCGGTTAGGGTTAGTTCGACCAAAACTTGGCCCTCTTGAACGCAAGATCAAATGCGGAAAGACTCCTGTTTTGGACGTTGGCACTCTTGCCAAAATCCGAAGTGGACACATCAAGGTGTATCCGGAGTTGAAACGGGTAATGCATTATTCGGCAGAGTTTGTTGATGGGAGAGTAGATAACTTCGACGCCATTATACTCGCCACGGGTTACAAAAGCAACGTACCCATGTGGCTAAAGGGAGTGAACATGTTTTCTGAGAAAGATGGATTTCCGCATAAACCATTTCCTAACGGTTGGAAAGGCGAAAGCGGATTGTATGCAGTCGGTTTCACAAAGCTTGGATTGCTTGGTGCAGCCATTGATGCCAAGAAGATCGCTGAGGATATTGAGGTTCAACGACATTTCTTACCATTGGCTCGTCCTCAACATTGTTAA

Protein Analysis

415

Amino Acids

46.54

Weight (kDa)

8.23

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lys_Orn_oxgnase PF13434 14 - 111 1.9e-08 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
FMO-like PF00743 27 - 256 1.1e-15 Flavin-binding monooxygenase-like
Pyr_redox_3 PF13738 43 - 123 1.6e-07 Pyridine nucleotide-disulphide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13260 AT4G13260 AT4G13260
fragaria_vesca FvH4_1g04800
malus_domestica MD01G1067600.v1.1 MD02G1046700.v1.1 MD15G1184800.v1.1
prunus_persica Prupe.7G231200_v2.0.a1
pyrus_communis pycom02g03910 pycom15g16630
rosa_chinensis RchiOBHm_Chr2g0090431
rosa_laevigata RLG00000016108
rosa_multiflora Rmu_sc0004514.1_g000016
rosa_roxburghii Rroxscaffold_2G00151160
rosa_rugosa Rorug02G0008000
rosa_samantha Rh2AG053400 Rh2BG052700 Rh2CG054800 Rh2DG053600
rosa_wichuraiana Rw2G004360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 364, 839
AccBSI CCGCTC 1 cut(s) 561
AccIII TCCGGA 1 cut(s) 934
AciI CCGC 6 cut(s) 122, 388, 561, 867, 1085, 1122
AclI AACGTT 2 cut(s) 20, 166
AclWI GGATC 2 cut(s) 35, 819
AcoI YGGCCR 1 cut(s) 114
AcvI CACGTG 1 cut(s) 760
AcyI GRCGYC 1 cut(s) 999
AdeI CACNNNGTG 1 cut(s) 763
AfaI GTAC 3 cut(s) 47, 681, 1035
AfiI CCNNNNNNNGG 3 cut(s) 364, 524, 839
AflIII ACRYGT 2 cut(s) 682, 1059
AgeI ACCGGT 1 cut(s) 768
AgsI TTSAA 5 cut(s) 270, 646, 851, 943, 1208
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AloI GAACNNNNNNTCC 2 cut(s) 362, 394
AluBI AGCT 4 cut(s) 247, 273, 549, 1150
AluI AGCT 4 cut(s) 247, 273, 549, 1150
Alw21I GWGCWC 1 cut(s) 759
Alw26I GTCTC 3 cut(s) 135, 428, 792
Alw44I GTGCAC 1 cut(s) 755
AlwI GGATC 2 cut(s) 35, 819
Aor13HI TCCGGA 1 cut(s) 934
AoxI GGCC 3 cut(s) 101, 114, 841
ApaLI GTGCAC 1 cut(s) 755
ApeKI GCWGC 2 cut(s) 193, 1166
ArsI GACNNNNNNTTYG 2 cut(s) 865, 897
AsiGI ACCGGT 1 cut(s) 768
AspS9I GGNCC 5 cut(s) 80, 101, 530, 766, 842
AsuC2I CCSGG 5 cut(s) 78, 100, 475, 518, 613
AsuHPI GGTGA 4 cut(s) 418, 505, 575, 815
AvaII GGWCC 3 cut(s) 80, 530, 766
BaeGI GKGCMC 1 cut(s) 759
BalI TGGCCA 1 cut(s) 116
BauI CACGAG 1 cut(s) 730
BbrPI CACGTG 1 cut(s) 760
BbsI GAAGAC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 759
BbvCI CCTCAGC 1 cut(s) 1191
BbvI GCAGC 2 cut(s) 205, 1178
BccI CCATC 4 cut(s) 503, 610, 971, 1070
BceAI ACGGC 1 cut(s) 88
BciVI GTATCC 1 cut(s) 942
BcnI CCSGG 5 cut(s) 78, 100, 475, 518, 613
BcoDI GTCTC 3 cut(s) 135, 428, 792
BfaI CTAG 4 cut(s) 60, 150, 161, 704
BfuI GTATCC 1 cut(s) 942
BglI GCCNNNNNGGC 1 cut(s) 122
BisI GCNGC 3 cut(s) 123, 194, 1167
BlsI GCNGC 3 cut(s) 124, 195, 1168
Bme1390I CCNGG 5 cut(s) 78, 100, 475, 518, 613
Bme18I GGWCC 3 cut(s) 80, 530, 766
BmgT120I GGNCC 5 cut(s) 80, 101, 530, 766, 842
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 5 cut(s) 78, 100, 475, 518, 613
BmsI GCATC 3 cut(s) 246, 486, 1165
BpiI GAAGAC 1 cut(s) 206
Bpu10I CCTNAGC 1 cut(s) 1191
BpuEI CTTGAG 3 cut(s) 335, 678, 725
BpuMI CCSGG 5 cut(s) 78, 100, 475, 518, 613
BsaAI YACGTR 4 cut(s) 49, 685, 760, 782
BsaHI GRCGYC 1 cut(s) 999
BsaJI CCNNGG 4 cut(s) 363, 474, 486, 1014
BsaWI WCCGGW 3 cut(s) 416, 768, 934
BsaXI ACNNNNNCTCC 6 cut(s) 210, 240, 522, 533, 552, 563
Bsc4I CCNNNNNNNGG 3 cut(s) 364, 524, 839
Bse118I RCCGGY 1 cut(s) 768
Bse1I ACTGG 2 cut(s) 117, 752
BseAI TCCGGA 1 cut(s) 934
BseDI CCNNGG 4 cut(s) 363, 474, 486, 1014
BseGI GGATG 4 cut(s) 144, 436, 605, 621
BseLI CCNNNNNNNGG 3 cut(s) 364, 524, 839
BseMII CTCAG 2 cut(s) 1059, 1182
BseNI ACTGG 2 cut(s) 117, 752
BseRI GAGGAG 1 cut(s) 503
BseSI GKGCMC 1 cut(s) 759
BseXI GCAGC 2 cut(s) 205, 1178
BsgI GTGCAG 1 cut(s) 1185
Bsh1285I CGRYCG 1 cut(s) 474
BshFI GGCC 3 cut(s) 103, 116, 843
BshTI ACCGGT 1 cut(s) 768
BsiEI CGRYCG 1 cut(s) 474
BsiHKAI GWGCWC 1 cut(s) 759
BsiSI CCGG 9 cut(s) 78, 99, 417, 474, 500, 518, 612, 769, 935
BsiWI CGTACG 1 cut(s) 45
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 3 cut(s) 364, 524, 839
BsmAI GTCTC 3 cut(s) 135, 428, 792
BsmFI GGGAC 1 cut(s) 123
BsmI GAATGC 1 cut(s) 487
BsnI GGCC 3 cut(s) 103, 116, 843
Bsp1286I GDGCHC 1 cut(s) 759
Bsp13I TCCGGA 1 cut(s) 934
Bsp143I GATC 5 cut(s) 40, 208, 811, 858, 1185
BspACI CCGC 6 cut(s) 122, 388, 561, 867, 1085, 1122
BspANI GGCC 3 cut(s) 103, 116, 843
BspCNI CTCAG 2 cut(s) 1060, 1183
BspEI TCCGGA 1 cut(s) 934
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 2 cut(s) 35, 819
BspQI GCTCTTC 1 cut(s) 657
BsrBI CCGCTC 1 cut(s) 561
BsrFI RCCGGY 1 cut(s) 768
BsrI ACTGG 2 cut(s) 117, 752
BssAI RCCGGY 1 cut(s) 768
BssECI CCNNGG 4 cut(s) 363, 474, 486, 1014
BssMI GATC 5 cut(s) 40, 208, 811, 858, 1185
BssNI GRCGYC 1 cut(s) 999
BssSI CACGAG 1 cut(s) 730
Bst2BI CACGAG 1 cut(s) 730
Bst4CI ACNGT 4 cut(s) 300, 364, 456, 1106
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 1 cut(s) 999
BstBAI YACGTR 4 cut(s) 49, 685, 760, 782
BstC8I GCNNGC 1 cut(s) 737
BstDEI CTNAG 2 cut(s) 1068, 1191
BstDSI CCRYGG 2 cut(s) 363, 1014
BstF5I GGATG 4 cut(s) 144, 436, 605, 621
BstKTI GATC 5 cut(s) 43, 211, 814, 861, 1188
BstMAI GTCTC 3 cut(s) 135, 428, 792
BstMBI GATC 5 cut(s) 40, 208, 811, 858, 1185
BstMCI CGRYCG 1 cut(s) 474
BstMWI GCNNNNNNNGC 3 cut(s) 122, 1156, 1175
BstNSI RCATGY 2 cut(s) 129, 1063
BstSCI CCNGG 5 cut(s) 76, 98, 473, 516, 611
BstSLI GKGCMC 1 cut(s) 759
BstSNI TACGTA 1 cut(s) 782
BstV1I GCAGC 2 cut(s) 205, 1178
BstV2I GAAGAC 1 cut(s) 206
BsuI GTATCC 1 cut(s) 942
BsuRI GGCC 3 cut(s) 103, 116, 843
BtgI CCRYGG 2 cut(s) 363, 1014
BtsCI GGATG 4 cut(s) 144, 436, 605, 621
BtsIMutI CAGTG 1 cut(s) 759
Cac8I GCNNGC 1 cut(s) 737
Cfr10I RCCGGY 1 cut(s) 768
Cfr13I GGNCC 5 cut(s) 80, 101, 530, 766, 842
CseI GACGC 2 cut(s) 171, 1007
Csp6I GTAC 3 cut(s) 46, 680, 1034
CspAI ACCGGT 1 cut(s) 768
CviAII CATG 4 cut(s) 38, 126, 1039, 1060
CviQI GTAC 3 cut(s) 46, 680, 1034
DdeI CTNAG 2 cut(s) 1068, 1191
DpnI GATC 5 cut(s) 42, 210, 813, 860, 1187
DpnII GATC 5 cut(s) 40, 208, 811, 858, 1185
DraI TTTAAA 1 cut(s) 132
DraIII CACNNNGTG 1 cut(s) 763
EaeI YGGCCR 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
Eco105I TACGTA 1 cut(s) 782
Eco32I GATATC 1 cut(s) 726
Eco47I GGWCC 3 cut(s) 80, 530, 766
Eco72I CACGTG 1 cut(s) 760
EcoRV GATATC 1 cut(s) 726
EcoT22I ATGCAT 1 cut(s) 957
FaeI CATG 4 cut(s) 41, 129, 1042, 1063
FaqI GGGAC 1 cut(s) 123
FatI CATG 4 cut(s) 37, 125, 1038, 1059
FauNDI CATATG 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 123, 194, 1167
FokI GGATG 4 cut(s) 131, 443, 612, 628
Fsp4HI GCNGC 3 cut(s) 123, 194, 1167
FspBI CTAG 4 cut(s) 60, 150, 161, 704
GluI GCNGC 3 cut(s) 123, 194, 1167
HaeIII GGCC 3 cut(s) 103, 116, 843
HapII CCGG 9 cut(s) 78, 99, 417, 474, 500, 518, 612, 769, 935
HgaI GACGC 2 cut(s) 171, 1007
Hin1I GRCGYC 1 cut(s) 999
Hin1II CATG 4 cut(s) 41, 129, 1042, 1063
HindIII AAGCTT 1 cut(s) 1148
HinfI GANTC 7 cut(s) 33, 73, 157, 317, 513, 674, 874
HpaII CCGG 9 cut(s) 78, 99, 417, 474, 500, 518, 612, 769, 935
HphI GGTGA 4 cut(s) 418, 505, 575, 815
Hpy166II GTNNAC 6 cut(s) 530, 682, 757, 766, 917, 1057
Hpy188I TCNGA 2 cut(s) 911, 1069
Hpy188III TCNNGA 8 cut(s) 108, 138, 161, 671, 695, 721, 848, 935
Hpy8I GTNNAC 6 cut(s) 530, 682, 757, 766, 917, 1057
Hpy99I CGWCG 2 cut(s) 109, 1001
HpyAV CCTTC 3 cut(s) 276, 421, 666
HpyCH4III ACNGT 4 cut(s) 300, 364, 456, 1106
HpyCH4IV ACGT 8 cut(s) 20, 48, 166, 684, 759, 781, 888, 1032
HpyCH4V TGCA 6 cut(s) 177, 639, 757, 955, 1133, 1166
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 1156, 1175
HpyF3I CTNAG 2 cut(s) 1068, 1191
HpySE526I ACGT 8 cut(s) 20, 48, 166, 684, 759, 781, 888, 1032
Hsp92I GRCGYC 1 cut(s) 999
Hsp92II CATG 4 cut(s) 41, 129, 1042, 1063
Kpn2I TCCGGA 1 cut(s) 934
Kzo9I GATC 5 cut(s) 40, 208, 811, 858, 1185
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 1 cut(s) 554
Lsp1109I GCAGC 2 cut(s) 205, 1178
LweI GCATC 3 cut(s) 246, 486, 1165
MaeI CTAG 4 cut(s) 60, 150, 161, 704
MaeII ACGT 8 cut(s) 20, 48, 166, 684, 759, 781, 888, 1032
MaeIII GTNAC 6 cut(s) 10, 183, 671, 777, 803, 1019
MalI GATC 5 cut(s) 42, 210, 813, 860, 1187
MbiI CCGCTC 1 cut(s) 561
MboI GATC 5 cut(s) 40, 208, 811, 858, 1185
MboII GAAGA 6 cut(s) 206, 436, 592, 595, 644, 1195
MhlI GDGCHC 1 cut(s) 759
MlsI TGGCCA 1 cut(s) 116
MluNI TGGCCA 1 cut(s) 116
MlyI GAGTC 3 cut(s) 326, 668, 868
MmeI TCCRAC 1 cut(s) 1088
Mox20I TGGCCA 1 cut(s) 116
Mph1103I ATGCAT 1 cut(s) 957
MroI TCCGGA 1 cut(s) 934
MscI TGGCCA 1 cut(s) 116
MseI TTAA 4 cut(s) 68, 131, 354, 1246
MslI CAYNNNNRTG 1 cut(s) 926
Msp20I TGGCCA 1 cut(s) 116
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 9 cut(s) 78, 99, 417, 474, 500, 518, 612, 769, 935
MspR9I CCNGG 5 cut(s) 78, 100, 475, 518, 613
Mva1269I GAATGC 1 cut(s) 487
MwoI GCNNNNNNNGC 3 cut(s) 122, 1156, 1175
NciI CCSGG 5 cut(s) 78, 100, 475, 518, 613
NdeI CATATG 1 cut(s) 205
NdeII GATC 5 cut(s) 40, 208, 811, 858, 1185
NlaIII CATG 4 cut(s) 41, 129, 1042, 1063
NlaIV GGNNCC 1 cut(s) 97
NmeAIII GCCGAG 1 cut(s) 511
NmuCI GTSAC 3 cut(s) 183, 671, 803
NsiI ATGCAT 1 cut(s) 957
NspI RCATGY 2 cut(s) 129, 1063
PciI ACATGT 1 cut(s) 1059
PciSI GCTCTTC 1 cut(s) 657
PctI GAATGC 1 cut(s) 487
PfeI GAWTC 4 cut(s) 33, 73, 157, 513
Pfl23II CGTACG 1 cut(s) 45
PflMI CCANNNNNTGG 2 cut(s) 364, 839
PfoI TCCNGGA 3 cut(s) 76, 516, 611
PinAI ACCGGT 1 cut(s) 768
PkrI GCNGC 3 cut(s) 124, 195, 1168
PleI GAGTC 3 cut(s) 325, 668, 868
PmaCI CACGTG 1 cut(s) 760
PmlI CACGTG 1 cut(s) 760
PpsI GAGTC 3 cut(s) 325, 668, 868
Ppu21I YACGTR 4 cut(s) 49, 685, 760, 782
PscI ACATGT 1 cut(s) 1059
Psp1406I AACGTT 2 cut(s) 20, 166
PspCI CACGTG 1 cut(s) 760
PspLI CGTACG 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 5 cut(s) 80, 101, 530, 766, 842
RsaI GTAC 3 cut(s) 47, 681, 1035
RsaNI GTAC 3 cut(s) 46, 680, 1034
RseI CAYNNNNRTG 1 cut(s) 926
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 4 cut(s) 68, 131, 354, 1246
SatI GCNGC 3 cut(s) 123, 194, 1167
Sau3AI GATC 5 cut(s) 40, 208, 811, 858, 1185
Sau96I GGNCC 5 cut(s) 80, 101, 530, 766, 842
SchI GAGTC 3 cut(s) 326, 668, 868
ScrFI CCNGG 5 cut(s) 78, 100, 475, 518, 613
SduI GDGCHC 1 cut(s) 759
SfaNI GCATC 3 cut(s) 246, 486, 1165
SinI GGWCC 3 cut(s) 80, 530, 766
SmiMI CAYNNNNRTG 1 cut(s) 926
SmlI CTYRAG 3 cut(s) 314, 693, 740
SmoI CTYRAG 3 cut(s) 314, 693, 740
SnaBI TACGTA 1 cut(s) 782
SsiI CCGC 6 cut(s) 122, 388, 561, 867, 1085, 1122
SspI AATATT 1 cut(s) 570
SspMI CTAG 4 cut(s) 60, 150, 161, 704
StyD4I CCNGG 5 cut(s) 76, 98, 473, 516, 611
TaaI ACNGT 4 cut(s) 300, 364, 456, 1106
TaiI ACGT 8 cut(s) 23, 51, 169, 687, 762, 784, 891, 1035
TaqI TCGA 6 cut(s) 137, 217, 381, 713, 829, 996
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 4 cut(s) 33, 73, 157, 513
Tru1I TTAA 4 cut(s) 68, 131, 354, 1246
Tru9I TTAA 4 cut(s) 68, 131, 354, 1246
TscAI CASTG 1 cut(s) 759
TseFI GTSAC 3 cut(s) 183, 671, 803
TseI GCWGC 2 cut(s) 193, 1166
Tsp45I GTSAC 3 cut(s) 183, 671, 803
TspDTI ATGAA 1 cut(s) 212
TspGWI ACGGA 2 cut(s) 33, 824
TspRI CASTG 1 cut(s) 759
Van91I CCANNNNNTGG 2 cut(s) 364, 839
VneI GTGCAC 1 cut(s) 755
VpaK11BI GGWCC 3 cut(s) 80, 530, 766
XbaI TCTAGA 1 cut(s) 160
XceI RCATGY 2 cut(s) 129, 1063
XspI CTAG 4 cut(s) 60, 150, 161, 704
Zsp2I ATGCAT 1 cut(s) 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.