Rmu_sc0004514.1_g000016

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004514.1
OG0010803
Physical Location & Seq
Reverse (-)
54668 .. 57239
2572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004514.1_g000016.1.cds

Sequence Viewer

Length: 1251 bp
atggcagatgctaaatcgacaaggcgtatatgggtcccggggccagtgatagtgggagcaggtccttccggcttagctgccgcggcttgtctcaaggaaaaaggtattccgagcttaatcctagaaaggaccaactgcatagcttcgttgtggcagcttaagacctacgaccgtctccgtcttcatctcccaaaacagttctgcgagctcatactcatgccattccctgcagatttcccaacctacccaaccaagaagcagttcgtgaattacttaaacgcttatgccgagcgctttgatctaaagccggctttcaacacgacggtggtgagtgccaagtacgattgtggttacgggctttggttggttaagactttggggttgaaaaatgaggaggcgattgagtatgtttgtcagtggctgatcgtcgccaccggagaaaatgccgaggaggttgtgccggagtttgaaggagtgagggactttggtgggcccattgtccataccagctcttacaagagcggcgagcgcttccgggggcagaaggtcctagtggtcggatgtgggaattcaggcatggaggtttgcttggatctctgcggttataatgcaaagccttcccttgtggttaaaaattcggtgcacatcttgccgcaagagatactgggaatatcgacttttgggctgtcgatgtggttgctcaagtggtttcccatgcgccttgtggatcgcttcttgcttctcgtctcacgcgttatgctgggcgacaccgctcgctttggaatcagccgccctcaccgtggccctctcgaactcaagtccatctctggcaagacacctgtgctcgatgttggcaccctcgctaagatcagaagtggtcacatcaaggtgtgcaaggcaatcaagcaagtaaaacatcaatcagtagagtttatagacgggaaagtggagtgtttcgacgccattattttggcaaccggttacagaagtaatgtgatgtcttcatggttgaaggaaacgagcatgtttagtgaaaaagatggattgccgcggaaggcatttccgaacggatggaaatgggagagcgggttgtattcggtggggtttacacagcgtgggctgttaggcgcttctctggatgcaaagagaattgcagaagatattgagcatcgctggaaagctgataacgccacccatctcatgccccgcttggtcacttgtgcttgtgcttctccactagcagaaccctag

Protein Analysis

416

Amino Acids

46.19

Weight (kDa)

9.08

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13260 AT4G13260 AT4G13260
fragaria_vesca FvH4_1g04800
malus_domestica MD01G1067600.v1.1 MD02G1046700.v1.1 MD15G1184800.v1.1
prunus_persica Prupe.7G231200_v2.0.a1
pyrus_communis pycom02g03910 pycom15g16630
rosa_chinensis RchiOBHm_Chr2g0090431
rosa_laevigata RLG00000016108
rosa_multiflora Rmu_sc0004514.1_g000016
rosa_roxburghii Rroxscaffold_2G00151160
rosa_rugosa Rorug02G0008000
rosa_samantha Rh2AG053400 Rh2BG052700 Rh2CG054800 Rh2DG053600
rosa_wichuraiana Rw2G004360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
Acc36I ACCTGC 1 cut(s) 50
AccB1I GGYRCC 1 cut(s) 854
AccBSI CCGCTC 3 cut(s) 522, 773, 1086
AccII CGCG 3 cut(s) 83, 753, 1051
AclWI GGATC 2 cut(s) 600, 735
AcsI RAATTY 2 cut(s) 568, 634
AcyI GRCGYC 1 cut(s) 960
AdeI CACNNNGTG 1 cut(s) 1115
AfaI GTAC 1 cut(s) 341
AfeI AGCGCT 2 cut(s) 293, 530
AfiI CCNNNNNNNGG 1 cut(s) 800
AflII CTTAAG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 751
AgeI ACCGGT 1 cut(s) 977
AgsI TTSAA 4 cut(s) 316, 385, 470, 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 7 cut(s) 77, 114, 143, 157, 208, 510, 1181
AluI AGCT 7 cut(s) 77, 114, 143, 157, 208, 510, 1181
Alw21I GWGCWC 3 cut(s) 210, 645, 846
Alw26I GTCTC 3 cut(s) 95, 179, 751
Alw44I GTGCAC 1 cut(s) 641
AlwI GGATC 2 cut(s) 600, 735
AlwNI CAGNNNCTG 1 cut(s) 421
Ama87I CYCGRG 1 cut(s) 37
Aor51HI AGCGCT 2 cut(s) 293, 530
AoxI GGCC 3 cut(s) 41, 491, 802
ApaI GGGCCC 1 cut(s) 495
ApaLI GTGCAC 1 cut(s) 641
ApeKI GCWGC 2 cut(s) 77, 154
ApoI RAATTY 2 cut(s) 568, 634
AsiGI ACCGGT 1 cut(s) 977
Asp700I GAANNNNTTC 1 cut(s) 260
AspLEI GCGC 4 cut(s) 294, 531, 720, 1130
AspS9I GGNCC 8 cut(s) 34, 41, 62, 129, 491, 492, 547, 803
AsuC2I CCSGG 3 cut(s) 38, 39, 536
AsuHPI GGTGA 2 cut(s) 340, 788
AvaI CYCGRG 1 cut(s) 37
AvaII GGWCC 4 cut(s) 34, 62, 129, 547
BaeGI GKGCMC 2 cut(s) 495, 645
BanI GGYRCC 1 cut(s) 854
BanII GRGCYC 2 cut(s) 210, 495
BbsI GAAGAC 2 cut(s) 173, 993
Bbv12I GWGCWC 3 cut(s) 210, 645, 846
BbvI GCAGC 2 cut(s) 64, 166
BccI CCATC 4 cut(s) 830, 1034, 1065, 1203
BcgI CGANNNNNNTGC 2 cut(s) 679, 713
BcnI CCSGG 3 cut(s) 38, 39, 536
BcoDI GTCTC 3 cut(s) 95, 179, 751
BfaI CTAG 4 cut(s) 122, 551, 1238, 1249
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 3 cut(s) 295, 532, 1131
BfrI CTTAAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 50
BisI GCNGC 8 cut(s) 78, 81, 84, 155, 523, 653, 790, 1049
BlpI GCTNAGC 1 cut(s) 73
BlsI GCNGC 8 cut(s) 79, 82, 85, 156, 524, 654, 791, 1050
Bme1390I CCNGG 3 cut(s) 38, 39, 536
Bme18I GGWCC 4 cut(s) 34, 62, 129, 547
BmeT110I CYCGRG 1 cut(s) 37
BmgT120I GGNCC 8 cut(s) 34, 41, 62, 129, 491, 492, 547, 803
BmiI GGNNCC 5 cut(s) 35, 36, 42, 493, 856
BmrFI CCNGG 3 cut(s) 38, 39, 536
BmrI ACTGGG 1 cut(s) 674
BmsI GCATC 2 cut(s) 1129, 1177
BmuI ACTGGG 1 cut(s) 674
BpiI GAAGAC 2 cut(s) 173, 993
Bpu1102I GCTNAGC 1 cut(s) 73
BpuEI CTTGAG 3 cut(s) 77, 686, 800
BpuMI CCSGG 3 cut(s) 38, 39, 536
BsaHI GRCGYC 1 cut(s) 960
BsaJI CCNNGG 7 cut(s) 37, 38, 81, 447, 535, 799, 1049
BsaWI WCCGGW 2 cut(s) 434, 977
BsaXI ACNNNNNCTCC 2 cut(s) 1073, 1103
Bsc4I CCNNNNNNNGG 1 cut(s) 800
Bse118I RCCGGY 2 cut(s) 307, 977
Bse1I ACTGG 2 cut(s) 44, 669
BseDI CCNNGG 7 cut(s) 37, 38, 81, 447, 535, 799, 1049
BseGI GGATG 3 cut(s) 566, 1076, 1144
BseLI CCNNNNNNNGG 1 cut(s) 800
BseNI ACTGG 2 cut(s) 44, 669
BseRI GAGGAG 2 cut(s) 407, 464
BseSI GKGCMC 2 cut(s) 495, 645
BseXI GCAGC 2 cut(s) 64, 166
BseYI CCCAGC 1 cut(s) 760
Bsh1236I CGCG 3 cut(s) 83, 753, 1051
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 3 cut(s) 43, 493, 804
BshNI GGYRCC 1 cut(s) 854
BshTI ACCGGT 1 cut(s) 977
BsiEI CGRYCG 1 cut(s) 172
BsiHKAI GWGCWC 3 cut(s) 210, 645, 846
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 7 cut(s) 38, 69, 308, 435, 461, 535, 978
BslFI GGGAC 2 cut(s) 20, 494
BslI CCNNNNNNNGG 1 cut(s) 800
BsmAI GTCTC 3 cut(s) 95, 179, 751
BsmBI CGTCTC 2 cut(s) 179, 751
BsmFI GGGAC 2 cut(s) 20, 494
BsnI GGCC 3 cut(s) 43, 493, 804
BsoBI CYCGRG 1 cut(s) 37
Bsp120I GGGCCC 1 cut(s) 491
Bsp1286I GDGCHC 4 cut(s) 210, 495, 645, 846
Bsp143I GATC 5 cut(s) 298, 423, 592, 727, 867
Bsp1720I GCTNAGC 1 cut(s) 73
BspANI GGCC 3 cut(s) 43, 493, 804
BspFNI CGCG 3 cut(s) 83, 753, 1051
BspLI GGNNCC 5 cut(s) 35, 36, 42, 493, 856
BspMAI CTGCAG 1 cut(s) 232
BspMI ACCTGC 1 cut(s) 50
BspPI GGATC 2 cut(s) 600, 735
BspT107I GGYRCC 1 cut(s) 854
BspTI CTTAAG 1 cut(s) 158
BsrBI CCGCTC 3 cut(s) 522, 773, 1086
BsrFI RCCGGY 2 cut(s) 307, 977
BsrI ACTGG 2 cut(s) 44, 669
BssAI RCCGGY 2 cut(s) 307, 977
BssECI CCNNGG 7 cut(s) 37, 38, 81, 447, 535, 799, 1049
BssMI GATC 5 cut(s) 298, 423, 592, 727, 867
BssNI GRCGYC 1 cut(s) 960
Bst4CI ACNGT 4 cut(s) 173, 198, 325, 800
BstACI GRCGYC 1 cut(s) 960
BstAFI CTTAAG 1 cut(s) 158
BstAPI GCANNNNNTGC 1 cut(s) 649
BstC8I GCNNGC 4 cut(s) 206, 309, 527, 775
BstDEI CTNAG 2 cut(s) 73, 864
BstDSI CCRYGG 3 cut(s) 81, 799, 1049
BstF5I GGATG 3 cut(s) 566, 1076, 1144
BstFNI CGCG 3 cut(s) 83, 753, 1051
BstH2I RGCGCY 3 cut(s) 295, 532, 1131
BstHHI GCGC 4 cut(s) 294, 531, 720, 1130
BstKTI GATC 5 cut(s) 301, 426, 595, 730, 870
BstMAI GTCTC 3 cut(s) 95, 179, 751
BstMBI GATC 5 cut(s) 298, 423, 592, 727, 867
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 4 cut(s) 83, 528, 649, 1187
BstNSI RCATGY 1 cut(s) 1027
BstSCI CCNGG 3 cut(s) 36, 37, 534
BstSFI CTRYAG 1 cut(s) 228
BstSLI GKGCMC 2 cut(s) 495, 645
BstUI CGCG 3 cut(s) 83, 753, 1051
BstV1I GCAGC 2 cut(s) 64, 166
BstV2I GAAGAC 2 cut(s) 173, 993
BstX2I RGATCY 1 cut(s) 592
BstXI CCANNNNNNTGG 1 cut(s) 970
BstYI RGATCY 1 cut(s) 592
BsuRI GGCC 3 cut(s) 43, 493, 804
BtgI CCRYGG 3 cut(s) 81, 799, 1049
BtgZI GCGATG 1 cut(s) 1154
BtsCI GGATG 3 cut(s) 566, 1076, 1144
BtsIMutI CAGTG 2 cut(s) 51, 422
BveI ACCTGC 1 cut(s) 50
Cac8I GCNNGC 4 cut(s) 206, 309, 527, 775
CaiI CAGNNNCTG 1 cut(s) 421
CfoI GCGC 4 cut(s) 294, 531, 720, 1130
Cfr10I RCCGGY 2 cut(s) 307, 977
Cfr13I GGNCC 8 cut(s) 34, 41, 62, 129, 491, 492, 547, 803
Cfr42I CCGCGG 2 cut(s) 84, 1052
Cfr9I CCCGGG 1 cut(s) 37
CseI GACGC 1 cut(s) 968
Csp6I GTAC 1 cut(s) 340
CspAI ACCGGT 1 cut(s) 977
CviAII CATG 6 cut(s) 217, 577, 715, 1005, 1024, 1201
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 2 cut(s) 73, 864
DpnI GATC 5 cut(s) 300, 425, 594, 729, 869
DpnII GATC 5 cut(s) 298, 423, 592, 727, 867
DraIII CACNNNGTG 1 cut(s) 1115
Ecl136II GAGCTC 1 cut(s) 208
Eco24I GRGCYC 2 cut(s) 210, 495
Eco47I GGWCC 4 cut(s) 34, 62, 129, 547
Eco47III AGCGCT 2 cut(s) 293, 530
Eco53kI GAGCTC 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 208
EcoO109I RGGNCCY 3 cut(s) 34, 62, 547
EcoRI GAATTC 1 cut(s) 568
EcoT38I GRGCYC 2 cut(s) 210, 495
Esp3I CGTCTC 2 cut(s) 179, 751
FaeI CATG 6 cut(s) 220, 580, 718, 1008, 1027, 1204
FaqI GGGAC 2 cut(s) 20, 494
FatI CATG 6 cut(s) 216, 576, 714, 1004, 1023, 1200
FauI CCCGC 2 cut(s) 1079, 1214
Fnu4HI GCNGC 8 cut(s) 78, 81, 84, 155, 523, 653, 790, 1049
FokI GGATG 3 cut(s) 573, 1083, 1151
FriOI GRGCYC 2 cut(s) 210, 495
Fsp4HI GCNGC 8 cut(s) 78, 81, 84, 155, 523, 653, 790, 1049
FspBI CTAG 4 cut(s) 122, 551, 1238, 1249
GlaI GCGC 4 cut(s) 293, 530, 719, 1129
GluI GCNGC 8 cut(s) 78, 81, 84, 155, 523, 653, 790, 1049
GsaI CCCAGC 1 cut(s) 764
HaeII RGCGCY 3 cut(s) 295, 532, 1131
HaeIII GGCC 3 cut(s) 43, 493, 804
HapII CCGG 7 cut(s) 38, 69, 308, 435, 461, 535, 978
HgaI GACGC 1 cut(s) 968
HhaI GCGC 4 cut(s) 294, 531, 720, 1130
Hin1I GRCGYC 1 cut(s) 960
Hin1II CATG 6 cut(s) 220, 580, 718, 1008, 1027, 1204
Hin6I GCGC 4 cut(s) 292, 529, 718, 1128
HinP1I GCGC 4 cut(s) 292, 529, 718, 1128
HinfI GANTC 1 cut(s) 783
HpaII CCGG 7 cut(s) 38, 69, 308, 435, 461, 535, 978
HphI GGTGA 2 cut(s) 340, 788
Hpy166II GTNNAC 2 cut(s) 643, 1107
Hpy188I TCNGA 4 cut(s) 111, 560, 872, 1065
Hpy188III TCNNGA 3 cut(s) 265, 809, 1136
Hpy8I GTNNAC 2 cut(s) 643, 1107
Hpy99I CGWCG 3 cut(s) 325, 431, 962
HpyAV CCTTC 6 cut(s) 75, 464, 538, 627, 1006, 1048
HpyCH4III ACNGT 4 cut(s) 173, 198, 325, 800
HpyCH4V TGCA 7 cut(s) 138, 230, 611, 643, 894, 1142, 1154
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 528, 649, 1187
HpyF3I CTNAG 2 cut(s) 73, 864
Hsp92I GRCGYC 1 cut(s) 960
Hsp92II CATG 6 cut(s) 220, 580, 718, 1008, 1027, 1204
HspAI GCGC 4 cut(s) 292, 529, 718, 1128
KflI GGGWCCC 1 cut(s) 34
KroI GCCGGC 1 cut(s) 307
KroNI GCCGGC 1 cut(s) 309
KspI CCGCGG 2 cut(s) 84, 1052
Kzo9I GATC 5 cut(s) 298, 423, 592, 727, 867
LmnI GCTCC 1 cut(s) 56
Lsp1109I GCAGC 2 cut(s) 64, 166
LweI GCATC 2 cut(s) 1129, 1177
MaeI CTAG 4 cut(s) 122, 551, 1238, 1249
MaeIII GTNAC 4 cut(s) 350, 878, 980, 1213
MalI GATC 5 cut(s) 300, 425, 594, 729, 869
MbiI CCGCTC 3 cut(s) 522, 773, 1086
MboI GATC 5 cut(s) 298, 423, 592, 727, 867
MboII GAAGA 3 cut(s) 173, 993, 1169
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 4 cut(s) 210, 495, 645, 846
MluCI AATT 4 cut(s) 268, 568, 634, 1149
MluI ACGCGT 1 cut(s) 751
MmeI TCCRAC 1 cut(s) 538
MnlI CCTC 9 cut(s) 385, 388, 442, 445, 471, 574, 804, 816, 869
MroNI GCCGGC 1 cut(s) 307
MroXI GAANNNNTTC 1 cut(s) 260
MseI TTAA 5 cut(s) 116, 159, 275, 369, 630
MslI CAYNNNNRTG 3 cut(s) 215, 323, 887
MspA1I CMGCKG 2 cut(s) 83, 1051
MspCI CTTAAG 1 cut(s) 158
MspI CCGG 7 cut(s) 38, 69, 308, 435, 461, 535, 978
MspR9I CCNGG 3 cut(s) 38, 39, 536
MvnI CGCG 3 cut(s) 83, 753, 1051
MwoI GCNNNNNNNGC 4 cut(s) 83, 528, 649, 1187
NaeI GCCGGC 1 cut(s) 309
NciI CCSGG 3 cut(s) 38, 39, 536
NdeII GATC 5 cut(s) 298, 423, 592, 727, 867
NgoMIV GCCGGC 1 cut(s) 307
NlaIII CATG 6 cut(s) 220, 580, 718, 1008, 1027, 1204
NlaIV GGNNCC 5 cut(s) 35, 36, 42, 493, 856
NmeAIII GCCGAG 2 cut(s) 313, 472
NmuCI GTSAC 2 cut(s) 878, 1213
NspI RCATGY 1 cut(s) 1027
OliI CACNNNNGTG 1 cut(s) 323
PcsI WCGNNNNNNNCGW 2 cut(s) 285, 750
PdiI GCCGGC 1 cut(s) 309
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 783
PinAI ACCGGT 1 cut(s) 977
PkrI GCNGC 8 cut(s) 79, 82, 85, 156, 524, 654, 791, 1050
PpuMI RGGWCCY 3 cut(s) 34, 62, 547
PsiI TTATAA 1 cut(s) 606
Psp124BI GAGCTC 1 cut(s) 210
Psp5II RGGWCCY 3 cut(s) 34, 62, 547
PspFI CCCAGC 1 cut(s) 760
PspN4I GGNNCC 5 cut(s) 35, 36, 42, 493, 856
PspOMI GGGCCC 1 cut(s) 491
PspPI GGNCC 8 cut(s) 34, 41, 62, 129, 491, 492, 547, 803
PspPPI RGGWCCY 3 cut(s) 34, 62, 547
PstI CTGCAG 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 421
PsuI RGATCY 1 cut(s) 592
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 3 cut(s) 215, 323, 887
SacI GAGCTC 1 cut(s) 210
SacII CCGCGG 2 cut(s) 84, 1052
SaqAI TTAA 5 cut(s) 116, 159, 275, 369, 630
SatI GCNGC 8 cut(s) 78, 81, 84, 155, 523, 653, 790, 1049
Sau3AI GATC 5 cut(s) 298, 423, 592, 727, 867
Sau96I GGNCC 8 cut(s) 34, 41, 62, 129, 491, 492, 547, 803
ScrFI CCNGG 3 cut(s) 38, 39, 536
SduI GDGCHC 4 cut(s) 210, 495, 645, 846
SfaNI GCATC 2 cut(s) 1129, 1177
SfcI CTRYAG 1 cut(s) 228
Sfr303I CCGCGG 2 cut(s) 84, 1052
SgrBI CCGCGG 2 cut(s) 84, 1052
SinI GGWCC 4 cut(s) 34, 62, 129, 547
SmaI CCCGGG 1 cut(s) 39
SmiMI CAYNNNNRTG 3 cut(s) 215, 323, 887
SmlI CTYRAG 4 cut(s) 92, 158, 701, 815
SmoI CTYRAG 4 cut(s) 92, 158, 701, 815
Sse9I AATT 4 cut(s) 268, 568, 634, 1149
SspMI CTAG 4 cut(s) 122, 551, 1238, 1249
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 3 cut(s) 36, 37, 534
TaaI ACNGT 4 cut(s) 173, 198, 325, 800
TaqI TCGA 6 cut(s) 17, 674, 689, 810, 846, 957
TasI AATT 4 cut(s) 268, 568, 634, 1149
TauI GCSGC 6 cut(s) 83, 86, 525, 655, 792, 1051
TfiI GAWTC 1 cut(s) 783
Tru1I TTAA 5 cut(s) 116, 159, 275, 369, 630
Tru9I TTAA 5 cut(s) 116, 159, 275, 369, 630
TscAI CASTG 2 cut(s) 51, 422
TseFI GTSAC 2 cut(s) 878, 1213
TseI GCWGC 2 cut(s) 77, 154
Tsp45I GTSAC 2 cut(s) 878, 1213
TspDTI ATGAA 2 cut(s) 173, 993
TspGWI ACGGA 2 cut(s) 167, 1083
TspMI CCCGGG 1 cut(s) 37
TspRI CASTG 2 cut(s) 51, 422
Vha464I CTTAAG 1 cut(s) 158
VneI GTGCAC 1 cut(s) 641
VpaK11BI GGWCC 4 cut(s) 34, 62, 129, 547
XapI RAATTY 2 cut(s) 568, 634
XceI RCATGY 1 cut(s) 1027
XcmI CCANNNNNNNNNTGG 1 cut(s) 721
XmaI CCCGGG 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 4 cut(s) 122, 551, 1238, 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.