Rorug02G0008000

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010803
Physical Location & Seq
Reverse (-)
681313 .. 684444
3132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0008000.1

Sequence Viewer

Length: 846 bp
ATGGCTGGGATCACCACCAGCTTCGCATCCAAGCCTATCCTATCCCGGACAACTCTCAAAACGACGCCGTTTCCATTCTCGCTTTCCTCCTTCGAGTCCAAGCTCTTCGGCCTTCGCTTTAATACCGTTTATCTGATAAATCCGAGCTCTTGTAGCTCTCTCTCCTCCGCCGTCAGCAGCCCAGTCACGGCCCGTTTCGGCGGCGGCGGGCCCCGGAGGTCGAGTAATGACCGCCGGAGACAGCAAAGCGATGCCGAGGATGATGATAAAGCACTTGATATCTCCACTATAAGGTCAGCTTCGGTGAGGCTTATTGATCAGAAGCAGGAAATGGTTGGAGTGGTGTCGAAGAGTGAGGCGATTCGAATGGCTGATGAGGCTGACCTTGATTTGGTAATATTGTCTCCAGATGCAGATCCTCCAGTTCTCAGGATTATGGATTACAATAAATATCGGTATGAGCAACAAAAGAAGAAAAGAGTGTCGCAGAAGAAAAGCGCAGCCAGTCGCATGGATTTGAAGGAGCTCAAAATGGGTTACAACATTGATCAACATGATTATTCTGTACGTATGAGAGCTGCTCAGAAGTTTCTGAAAGATGGTGATAAGGTCAAGGTTATGGTCAACTTGAAAGGTCGGGAAAATGAGTTCCGAAATAAAGCTATCGAGCTTCTCAAACGCTTTCAAAGTGAAGTTGGGGAGCTTGCAACAGAGGAAGCCAAAAGCTTCAGCGACAGAAACATGTTTATAGTTTTAGTTCCAAACAAGGCTATTCTCCAGAAAGCTCAAGAGCCGTCAAAGAAAAAGGAGAACCCTGCGGTGAATGAAGTATCATCAAGTGTCTGA

Protein Analysis

281

Amino Acids

31.5

Weight (kDa)

9.64

Isoelectric Point (pI)

57.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF3_N PF05198 96 - 160 3.6e-27 Translation initiation factor IF-3, N-terminal domain
IF3_C PF00707 172 - 255 1.5e-24 Translation initiation factor IF-3, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13260 AT4G13260 AT4G13260
fragaria_vesca FvH4_1g04800
malus_domestica MD01G1067600.v1.1 MD02G1046700.v1.1 MD15G1184800.v1.1
prunus_persica Prupe.7G231200_v2.0.a1
pyrus_communis pycom02g03910 pycom15g16630
rosa_chinensis RchiOBHm_Chr2g0090431
rosa_laevigata RLG00000016108
rosa_multiflora Rmu_sc0004514.1_g000016
rosa_roxburghii Rroxscaffold_2G00151160
rosa_rugosa Rorug02G0008000
rosa_samantha Rh2AG053400 Rh2BG052700 Rh2CG054800 Rh2DG053600
rosa_wichuraiana Rw2G004360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 168, 201, 204, 207, 232, 818
AclWI GGATC 2 cut(s) 17, 410
AcuI CTGAAG 1 cut(s) 712
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 1 cut(s) 567
AfiI CCNNNNNNNGG 3 cut(s) 187, 291, 391
AflIII ACRYGT 1 cut(s) 741
AgsI TTSAA 3 cut(s) 520, 631, 686
AloI GAACNNNNNNTCC 2 cut(s) 632, 664
Alw21I GWGCWC 2 cut(s) 149, 528
Alw26I GTCTC 2 cut(s) 232, 408
AlwI GGATC 2 cut(s) 17, 410
AoxI GGCC 3 cut(s) 109, 189, 209
ApaI GGGCCC 1 cut(s) 213
ApeKI GCWGC 3 cut(s) 177, 500, 578
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 3 cut(s) 190, 209, 210
AsuC2I CCSGG 2 cut(s) 46, 214
AsuHPI GGTGA 4 cut(s) 4, 316, 614, 832
AsuII TTCGAA 1 cut(s) 364
BaeGI GKGCMC 1 cut(s) 213
BanII GRGCYC 3 cut(s) 149, 213, 528
Bbv12I GWGCWC 2 cut(s) 149, 528
BbvI GCAGC 3 cut(s) 189, 512, 565
BccI CCATC 1 cut(s) 593
BceAI ACGGC 4 cut(s) 52, 155, 204, 778
BclI TGATCA 2 cut(s) 316, 547
BcnI CCSGG 2 cut(s) 46, 214
BcoDI GTCTC 2 cut(s) 232, 408
BisI GCNGC 5 cut(s) 178, 202, 205, 501, 579
BlsI GCNGC 5 cut(s) 179, 203, 206, 502, 580
Bme1390I CCNGG 2 cut(s) 46, 214
BmgT120I GGNCC 3 cut(s) 190, 209, 210
BmiI GGNNCC 2 cut(s) 211, 212
BmrFI CCNGG 2 cut(s) 46, 214
BmrI ACTGGG 1 cut(s) 176
BmsI GCATC 3 cut(s) 35, 241, 400
BmuI ACTGGG 1 cut(s) 176
BplI GAGNNNNNCTC 2 cut(s) 565, 597
BpmI CTGGAG 3 cut(s) 390, 405, 761
Bpu14I TTCGAA 1 cut(s) 364
BpuEI CTTGAG 1 cut(s) 771
BpuMI CCSGG 2 cut(s) 46, 214
BsaAI YACGTR 1 cut(s) 569
BsaBI GATNNNNATC 1 cut(s) 414
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 212, 255
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 291, 391
Bse1I ACTGG 3 cut(s) 182, 422, 504
Bse8I GATNNNNATC 1 cut(s) 414
BseDI CCNNGG 2 cut(s) 212, 255
BseGI GGATG 2 cut(s) 26, 265
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 3 cut(s) 187, 291, 391
BseMII CTCAG 2 cut(s) 442, 596
BseNI ACTGG 3 cut(s) 182, 422, 504
BseRI GAGGAG 1 cut(s) 154
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 3 cut(s) 189, 512, 565
BseYI CCCAGC 1 cut(s) 5
BshFI GGCC 3 cut(s) 111, 191, 211
BsiHKAI GWGCWC 2 cut(s) 149, 528
BsiSI CCGG 3 cut(s) 46, 214, 235
BslI CCNNNNNNNGG 3 cut(s) 187, 291, 391
BsmAI GTCTC 2 cut(s) 232, 408
BsnI GGCC 3 cut(s) 111, 191, 211
Bsp119I TTCGAA 1 cut(s) 364
Bsp120I GGGCCC 1 cut(s) 209
Bsp1286I GDGCHC 3 cut(s) 149, 213, 528
Bsp143I GATC 4 cut(s) 9, 316, 415, 547
BspACI CCGC 6 cut(s) 168, 201, 204, 207, 232, 818
BspANI GGCC 3 cut(s) 111, 191, 211
BspCNI CTCAG 2 cut(s) 441, 595
BspLI GGNNCC 2 cut(s) 211, 212
BspPI GGATC 2 cut(s) 17, 410
BspQI GCTCTTC 1 cut(s) 110
BspT104I TTCGAA 1 cut(s) 364
BsrI ACTGG 3 cut(s) 182, 422, 504
BssECI CCNNGG 2 cut(s) 212, 255
BssMI GATC 4 cut(s) 9, 316, 415, 547
BssNI GRCGYC 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 127
Bst6I CTCTTC 2 cut(s) 110, 344
BstACI GRCGYC 1 cut(s) 65
BstBAI YACGTR 1 cut(s) 569
BstBI TTCGAA 1 cut(s) 364
BstC8I GCNNGC 2 cut(s) 209, 705
BstDEI CTNAG 2 cut(s) 428, 582
BstF5I GGATG 2 cut(s) 26, 265
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 4 cut(s) 12, 319, 418, 550
BstMAI GTCTC 2 cut(s) 232, 408
BstMBI GATC 4 cut(s) 9, 316, 415, 547
BstMWI GCNNNNNNNGC 2 cut(s) 153, 377
BstNSI RCATGY 1 cut(s) 745
BstSCI CCNGG 2 cut(s) 44, 212
BstSLI GKGCMC 1 cut(s) 213
BstSNI TACGTA 1 cut(s) 569
BstV1I GCAGC 3 cut(s) 189, 512, 565
BstX2I RGATCY 1 cut(s) 415
BstXI CCANNNNNNTGG 1 cut(s) 511
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 3 cut(s) 111, 191, 211
BtgZI GCGATG 1 cut(s) 264
BtsCI GGATG 2 cut(s) 26, 265
Cac8I GCNNGC 2 cut(s) 209, 705
CfoI GCGC 1 cut(s) 500
Cfr13I GGNCC 3 cut(s) 190, 209, 210
CseI GACGC 1 cut(s) 73
Csp6I GTAC 1 cut(s) 566
CviAII CATG 3 cut(s) 511, 554, 742
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 2 cut(s) 428, 582
DpnI GATC 4 cut(s) 11, 318, 417, 549
DpnII GATC 4 cut(s) 9, 316, 415, 547
Eam1104I CTCTTC 2 cut(s) 110, 344
EarI CTCTTC 2 cut(s) 110, 344
EciI GGCGGA 1 cut(s) 157
Ecl136II GAGCTC 2 cut(s) 147, 526
Eco105I TACGTA 1 cut(s) 569
Eco24I GRGCYC 3 cut(s) 149, 213, 528
Eco32I GATATC 1 cut(s) 280
Eco53kI GAGCTC 2 cut(s) 147, 526
Eco57I CTGAAG 1 cut(s) 712
EcoICRI GAGCTC 2 cut(s) 147, 526
EcoO109I RGGNCCY 1 cut(s) 210
EcoRV GATATC 1 cut(s) 280
EcoT38I GRGCYC 3 cut(s) 149, 213, 528
FaeI CATG 3 cut(s) 514, 557, 745
FaiI YATR 9 cut(s) 290, 437, 459, 512, 555, 572, 620, 743, 749
FalI AAGNNNNNCTT 2 cut(s) 283, 315
FatI CATG 3 cut(s) 510, 553, 741
FauI CCCGC 1 cut(s) 200
FbaI TGATCA 2 cut(s) 316, 547
Fnu4HI GCNGC 5 cut(s) 178, 202, 205, 501, 579
FokI GGATG 2 cut(s) 13, 272
FriOI GRGCYC 3 cut(s) 149, 213, 528
Fsp4HI GCNGC 5 cut(s) 178, 202, 205, 501, 579
GlaI GCGC 1 cut(s) 499
GluI GCNGC 5 cut(s) 178, 202, 205, 501, 579
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 3 cut(s) 390, 405, 761
HaeIII GGCC 3 cut(s) 111, 191, 211
HapII CCGG 3 cut(s) 46, 214, 235
HgaI GACGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 500
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 3 cut(s) 514, 557, 745
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 1 cut(s) 724
HinfI GANTC 2 cut(s) 95, 361
HpaII CCGG 3 cut(s) 46, 214, 235
HphI GGTGA 4 cut(s) 4, 316, 614, 832
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 7 cut(s) 135, 144, 321, 585, 594, 653, 845
Hpy188III TCNNGA 5 cut(s) 407, 430, 638, 778, 788
Hpy8I GTNNAC 1 cut(s) 625
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 3 cut(s) 100, 122, 514
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4IV ACGT 1 cut(s) 568
HpyCH4V TGCA 2 cut(s) 413, 707
HpyF10VI GCNNNNNNNGC 2 cut(s) 153, 377
HpyF3I CTNAG 2 cut(s) 428, 582
HpySE526I ACGT 1 cut(s) 568
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 3 cut(s) 514, 557, 745
HspAI GCGC 1 cut(s) 498
Ksp22I TGATCA 2 cut(s) 316, 547
Kzo9I GATC 4 cut(s) 9, 316, 415, 547
LguI GCTCTTC 1 cut(s) 110
LmnI GCTCC 2 cut(s) 523, 700
Lsp1109I GCAGC 3 cut(s) 189, 512, 565
LweI GCATC 3 cut(s) 35, 241, 400
MaeII ACGT 1 cut(s) 568
MaeIII GTNAC 2 cut(s) 184, 536
MalI GATC 4 cut(s) 11, 318, 417, 549
MboI GATC 4 cut(s) 9, 316, 415, 547
MboII GAAGA 4 cut(s) 97, 361, 484, 502
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 3 cut(s) 149, 213, 528
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 9 cut(s) 97, 175, 210, 250, 300, 349, 370, 429, 706
MseI TTAA 1 cut(s) 120
MspI CCGG 3 cut(s) 46, 214, 235
MspR9I CCNGG 2 cut(s) 46, 214
MwoI GCNNNNNNNGC 2 cut(s) 153, 377
NciI CCSGG 2 cut(s) 46, 214
NdeII GATC 4 cut(s) 9, 316, 415, 547
NlaIII CATG 3 cut(s) 514, 557, 745
NlaIV GGNNCC 2 cut(s) 211, 212
NmeAIII GCCGAG 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 184
NspI RCATGY 1 cut(s) 745
NspV TTCGAA 1 cut(s) 364
PciI ACATGT 1 cut(s) 741
PciSI GCTCTTC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 361
PfoI TCCNGGA 1 cut(s) 44
PkrI GCNGC 5 cut(s) 179, 203, 206, 502, 580
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Ppu21I YACGTR 1 cut(s) 569
PscI ACATGT 1 cut(s) 741
Psp124BI GAGCTC 2 cut(s) 149, 528
PspFI CCCAGC 1 cut(s) 5
PspN4I GGNNCC 2 cut(s) 211, 212
PspOMI GGGCCC 1 cut(s) 209
PspPI GGNCC 3 cut(s) 190, 209, 210
PsuI RGATCY 1 cut(s) 415
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
SacI GAGCTC 2 cut(s) 149, 528
SapI GCTCTTC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 5 cut(s) 178, 202, 205, 501, 579
Sau3AI GATC 4 cut(s) 9, 316, 415, 547
Sau96I GGNCC 3 cut(s) 190, 209, 210
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 2 cut(s) 46, 214
SduI GDGCHC 3 cut(s) 149, 213, 528
SfaNI GCATC 3 cut(s) 35, 241, 400
SfuI TTCGAA 1 cut(s) 364
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
SnaBI TACGTA 1 cut(s) 569
SsiI CCGC 6 cut(s) 168, 201, 204, 207, 232, 818
SspI AATATT 1 cut(s) 399
SstI GAGCTC 2 cut(s) 149, 528
StyD4I CCNGG 2 cut(s) 44, 212
TaaI ACNGT 1 cut(s) 127
TaiI ACGT 1 cut(s) 571
TaqI TCGA 5 cut(s) 93, 221, 347, 364, 666
TauI GCSGC 2 cut(s) 204, 207
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 3 cut(s) 177, 500, 578
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 1 cut(s) 840
XceI RCATGY 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.