Rh2AG053400

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010803
Physical Location & Seq
Reverse (-)
4224018 .. 4227075
3058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG053400.1

Sequence Viewer

Length: 1107 bp
ATGGACTTTCTGAAGGAGCTGGAAGGCAAACGAGTGCATGATCCTTTGATTCATTTCCAATCCAAAACAGACATGGCAGGTGCTAAATCGACAAGGAGTATATGGGTCCCGGGGCCAGTGATAGTGGGAGCAGGTCCTTCCGGCTTAGCTGCCGCTGCTTGTCTCAAGGAAAAAGGTATTCCGAGCTTAATCCTAGAAAGGACCAACTGCATAGCTTCGTTGTGGCAGCTTAAGACCTACGACCGTCTCCGTCTTCATCTCCCAAAACAGTTCTGCGAGCTCATACTCATGCCATTCCCTGCAGATTTCCCAACCTACCCAACCAAGAAGCAGTTCGTGAATTACTTAAACGATTATGCCGAGCGCTTTGATCTAAAGCCGGCTTTCAACACGACGGTGGTGAGTGCCAAGTACGATAGTCGTTGCGGGCTTTGGTTGGTTAACACTTTGGGGTTGAAAAATGAGGAGGCGATTGAGTATGTTTGTCAGTGGCTGATCGTCGCCACCGGAGAAAATGCCGAGGAGGTTTTGCCGGAGTTTGAAGGAGTGAGGGACTTTGGTGGGCCCATTGTCCATACCAGCTCTTACAAGAGCGGCGAGCGCTTCGGGGGGCAGAAGGTCCTAGTGGTCGGATGTGGGAATTCAGGCATGGAGGTTTGCTTGGATCTCTGCGATTATAATGCAAAGCCTTCCCTTGTGGTTAAAAATTCGGTGCACATCTTGCCGCAAGAGATACTGGGAATATCGACTTTTGGGCTGTCGATGTGGTTGCTCAAGTGGTTTCCCATGCGCCTTGTGGATCGCTTCTTGCTTCTCGTCTCACGCGTTATGCTGGGCGACACCGCTCGCTTTGGAATCAGCCGCCCTCACCGTGGCCCTCTCGAACTCAAGTCCATCTCTGGCAAGACACCTGTGCTCGATGTTGGCACCCTCGCTAAGATCAGAAGTGGTCACATCAAGGTGTGCAAGGCAATCAAGCAAGTAAAACATCAATCAGTAGAGTTTATAGACGGGAAAGTGGAGTGTTTCGACGCCATTATTTTGGCAACCGGTTACAGAAGTAATGTGATGTCTTGGTTGAAGGTTGATATTGATGACTTGCTTTGA

Protein Analysis

368

Amino Acids

41.0

Weight (kDa)

8.58

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_3 PF13738 40 - 237 1.8e-22 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_2 PF07992 40 - 234 2.2e-10 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 41 - 353 1.3e-28 Flavin-binding monooxygenase-like
Lys_Orn_oxgnase PF13434 105 - 231 1.4e-11 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13260 AT4G13260 AT4G13260
fragaria_vesca FvH4_1g04800
malus_domestica MD01G1067600.v1.1 MD02G1046700.v1.1 MD15G1184800.v1.1
prunus_persica Prupe.7G231200_v2.0.a1
pyrus_communis pycom02g03910 pycom15g16630
rosa_chinensis RchiOBHm_Chr2g0090431
rosa_laevigata RLG00000016108
rosa_multiflora Rmu_sc0004514.1_g000016
rosa_roxburghii Rroxscaffold_2G00151160
rosa_rugosa Rorug02G0008000
rosa_samantha Rh2AG053400 Rh2BG052700 Rh2CG054800 Rh2DG053600
rosa_wichuraiana Rw2G004360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
AarI CACCTGC 1 cut(s) 68
Acc36I ACCTGC 2 cut(s) 68, 122
AccB1I GGYRCC 1 cut(s) 926
AccBSI CCGCTC 2 cut(s) 594, 845
AccII CGCG 1 cut(s) 825
AciI CCGC 6 cut(s) 153, 426, 594, 725, 843, 862
AclWI GGATC 3 cut(s) 35, 672, 807
AcsI RAATTY 2 cut(s) 640, 706
AcuI CTGAAG 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 1032
AfaI GTAC 1 cut(s) 413
AfeI AGCGCT 2 cut(s) 365, 602
AfiI CCNNNNNNNGG 1 cut(s) 872
AflII CTTAAG 1 cut(s) 230
AflIII ACRYGT 1 cut(s) 823
AgeI ACCGGT 1 cut(s) 1049
AgsI TTSAA 4 cut(s) 388, 457, 542, 1081
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AleI CACNNNNGTG 1 cut(s) 395
AluBI AGCT 7 cut(s) 19, 149, 186, 215, 229, 280, 582
AluI AGCT 7 cut(s) 19, 149, 186, 215, 229, 280, 582
Alw21I GWGCWC 3 cut(s) 282, 717, 918
Alw26I GTCTC 3 cut(s) 167, 251, 823
Alw44I GTGCAC 1 cut(s) 713
AlwI GGATC 3 cut(s) 35, 672, 807
AlwNI CAGNNNCTG 1 cut(s) 493
Ama87I CYCGRG 1 cut(s) 109
Aor51HI AGCGCT 2 cut(s) 365, 602
AoxI GGCC 3 cut(s) 113, 563, 874
ApaI GGGCCC 1 cut(s) 567
ApaLI GTGCAC 1 cut(s) 713
ApeKI GCWGC 3 cut(s) 149, 155, 226
ApoI RAATTY 2 cut(s) 640, 706
AsiGI ACCGGT 1 cut(s) 1049
Asp700I GAANNNNTTC 1 cut(s) 332
AspLEI GCGC 3 cut(s) 366, 603, 792
AspS9I GGNCC 8 cut(s) 106, 113, 134, 201, 563, 564, 619, 875
AsuC2I CCSGG 2 cut(s) 110, 111
AsuHPI GGTGA 2 cut(s) 412, 860
AvaI CYCGRG 1 cut(s) 109
AvaII GGWCC 4 cut(s) 106, 134, 201, 619
BaeGI GKGCMC 2 cut(s) 567, 717
BanI GGYRCC 1 cut(s) 926
BanII GRGCYC 2 cut(s) 282, 567
BbsI GAAGAC 1 cut(s) 245
Bbv12I GWGCWC 3 cut(s) 282, 717, 918
BbvI GCAGC 3 cut(s) 136, 142, 238
BccI CCATC 1 cut(s) 902
BcgI CGANNNNNNTGC 4 cut(s) 662, 696, 751, 785
BcnI CCSGG 2 cut(s) 110, 111
BcoDI GTCTC 3 cut(s) 167, 251, 823
BfaI CTAG 2 cut(s) 194, 623
BfmI CTRYAG 1 cut(s) 300
BfoI RGCGCY 2 cut(s) 367, 604
BfrI CTTAAG 1 cut(s) 230
BfuAI ACCTGC 2 cut(s) 68, 122
BisI GCNGC 7 cut(s) 150, 153, 156, 227, 595, 725, 862
BlpI GCTNAGC 1 cut(s) 145
BlsI GCNGC 7 cut(s) 151, 154, 157, 228, 596, 726, 863
Bme1390I CCNGG 2 cut(s) 110, 111
Bme18I GGWCC 4 cut(s) 106, 134, 201, 619
BmeT110I CYCGRG 1 cut(s) 109
BmgT120I GGNCC 8 cut(s) 106, 113, 134, 201, 563, 564, 619, 875
BmiI GGNNCC 5 cut(s) 107, 108, 114, 565, 928
BmrFI CCNGG 2 cut(s) 110, 111
BmrI ACTGGG 1 cut(s) 746
BmuI ACTGGG 1 cut(s) 746
BpiI GAAGAC 1 cut(s) 245
Bpu1102I GCTNAGC 1 cut(s) 145
BpuEI CTTGAG 3 cut(s) 149, 758, 872
BpuMI CCSGG 2 cut(s) 110, 111
BsaHI GRCGYC 1 cut(s) 1032
BsaJI CCNNGG 4 cut(s) 109, 110, 519, 871
BsaWI WCCGGW 2 cut(s) 506, 1049
Bsc4I CCNNNNNNNGG 1 cut(s) 872
Bse118I RCCGGY 2 cut(s) 379, 1049
Bse1I ACTGG 2 cut(s) 116, 741
BseDI CCNNGG 4 cut(s) 109, 110, 519, 871
BseGI GGATG 1 cut(s) 638
BseLI CCNNNNNNNGG 1 cut(s) 872
BseNI ACTGG 2 cut(s) 116, 741
BseRI GAGGAG 2 cut(s) 479, 536
BseSI GKGCMC 2 cut(s) 567, 717
BseXI GCAGC 3 cut(s) 136, 142, 238
BseYI CCCAGC 1 cut(s) 832
Bsh1236I CGCG 1 cut(s) 825
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 3 cut(s) 115, 565, 876
BshNI GGYRCC 1 cut(s) 926
BshTI ACCGGT 1 cut(s) 1049
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 3 cut(s) 282, 717, 918
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 6 cut(s) 110, 141, 380, 507, 533, 1050
BslFI GGGAC 2 cut(s) 92, 566
BslI CCNNNNNNNGG 1 cut(s) 872
BsmAI GTCTC 3 cut(s) 167, 251, 823
BsmBI CGTCTC 2 cut(s) 251, 823
BsmFI GGGAC 2 cut(s) 92, 566
BsnI GGCC 3 cut(s) 115, 565, 876
BsoBI CYCGRG 1 cut(s) 109
Bsp120I GGGCCC 1 cut(s) 563
Bsp1286I GDGCHC 4 cut(s) 282, 567, 717, 918
Bsp143I GATC 6 cut(s) 40, 370, 495, 664, 799, 939
Bsp1720I GCTNAGC 1 cut(s) 145
BspACI CCGC 6 cut(s) 153, 426, 594, 725, 843, 862
BspANI GGCC 3 cut(s) 115, 565, 876
BspFNI CGCG 1 cut(s) 825
BspLI GGNNCC 5 cut(s) 107, 108, 114, 565, 928
BspMAI CTGCAG 1 cut(s) 304
BspMI ACCTGC 2 cut(s) 68, 122
BspPI GGATC 3 cut(s) 35, 672, 807
BspT107I GGYRCC 1 cut(s) 926
BspTI CTTAAG 1 cut(s) 230
BsrBI CCGCTC 2 cut(s) 594, 845
BsrFI RCCGGY 2 cut(s) 379, 1049
BsrI ACTGG 2 cut(s) 116, 741
BssAI RCCGGY 2 cut(s) 379, 1049
BssECI CCNNGG 4 cut(s) 109, 110, 519, 871
BssMI GATC 6 cut(s) 40, 370, 495, 664, 799, 939
BssNI GRCGYC 1 cut(s) 1032
Bst4CI ACNGT 4 cut(s) 245, 270, 397, 872
BstACI GRCGYC 1 cut(s) 1032
BstAFI CTTAAG 1 cut(s) 230
BstAPI GCANNNNNTGC 1 cut(s) 721
BstC8I GCNNGC 5 cut(s) 278, 381, 428, 599, 847
BstDEI CTNAG 2 cut(s) 145, 936
BstDSI CCRYGG 1 cut(s) 871
BstF5I GGATG 1 cut(s) 638
BstFNI CGCG 1 cut(s) 825
BstH2I RGCGCY 2 cut(s) 367, 604
BstHHI GCGC 3 cut(s) 366, 603, 792
BstKTI GATC 6 cut(s) 43, 373, 498, 667, 802, 942
BstMAI GTCTC 3 cut(s) 167, 251, 823
BstMBI GATC 6 cut(s) 40, 370, 495, 664, 799, 939
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 3 cut(s) 155, 600, 721
BstSCI CCNGG 2 cut(s) 108, 109
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 2 cut(s) 567, 717
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 3 cut(s) 136, 142, 238
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 1 cut(s) 664
BstXI CCANNNNNNTGG 1 cut(s) 1042
BstYI RGATCY 1 cut(s) 664
BsuRI GGCC 3 cut(s) 115, 565, 876
BtgI CCRYGG 1 cut(s) 871
BtsCI GGATG 1 cut(s) 638
BtsIMutI CAGTG 2 cut(s) 123, 494
BveI ACCTGC 2 cut(s) 68, 122
Cac8I GCNNGC 5 cut(s) 278, 381, 428, 599, 847
CaiI CAGNNNCTG 1 cut(s) 493
CfoI GCGC 3 cut(s) 366, 603, 792
Cfr10I RCCGGY 2 cut(s) 379, 1049
Cfr13I GGNCC 8 cut(s) 106, 113, 134, 201, 563, 564, 619, 875
Cfr9I CCCGGG 1 cut(s) 109
CseI GACGC 1 cut(s) 1040
Csp6I GTAC 1 cut(s) 412
CspAI ACCGGT 1 cut(s) 1049
CviAII CATG 5 cut(s) 38, 73, 289, 649, 787
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 2 cut(s) 145, 936
DpnI GATC 6 cut(s) 42, 372, 497, 666, 801, 941
DpnII GATC 6 cut(s) 40, 370, 495, 664, 799, 939
Ecl136II GAGCTC 1 cut(s) 280
Eco24I GRGCYC 2 cut(s) 282, 567
Eco47I GGWCC 4 cut(s) 106, 134, 201, 619
Eco47III AGCGCT 2 cut(s) 365, 602
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 3 cut(s) 106, 134, 619
EcoRI GAATTC 1 cut(s) 640
EcoT38I GRGCYC 2 cut(s) 282, 567
Esp3I CGTCTC 2 cut(s) 251, 823
FaeI CATG 5 cut(s) 41, 76, 292, 652, 790
FaqI GGGAC 2 cut(s) 92, 566
FatI CATG 5 cut(s) 37, 72, 288, 648, 786
FauI CCCGC 1 cut(s) 419
Fnu4HI GCNGC 7 cut(s) 150, 153, 156, 227, 595, 725, 862
FokI GGATG 1 cut(s) 645
FriOI GRGCYC 2 cut(s) 282, 567
Fsp4HI GCNGC 7 cut(s) 150, 153, 156, 227, 595, 725, 862
FspBI CTAG 2 cut(s) 194, 623
GlaI GCGC 3 cut(s) 365, 602, 791
GluI GCNGC 7 cut(s) 150, 153, 156, 227, 595, 725, 862
GsaI CCCAGC 1 cut(s) 836
HaeII RGCGCY 2 cut(s) 367, 604
HaeIII GGCC 3 cut(s) 115, 565, 876
HapII CCGG 6 cut(s) 110, 141, 380, 507, 533, 1050
HgaI GACGC 1 cut(s) 1040
HhaI GCGC 3 cut(s) 366, 603, 792
Hin1I GRCGYC 1 cut(s) 1032
Hin1II CATG 5 cut(s) 41, 76, 292, 652, 790
Hin6I GCGC 3 cut(s) 364, 601, 790
HinP1I GCGC 3 cut(s) 364, 601, 790
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HinfI GANTC 2 cut(s) 49, 855
HpaI GTTAAC 1 cut(s) 442
HpaII CCGG 6 cut(s) 110, 141, 380, 507, 533, 1050
HphI GGTGA 2 cut(s) 412, 860
Hpy166II GTNNAC 2 cut(s) 442, 715
Hpy188I TCNGA 4 cut(s) 12, 183, 632, 944
Hpy188III TCNNGA 2 cut(s) 337, 881
Hpy8I GTNNAC 2 cut(s) 442, 715
Hpy99I CGWCG 3 cut(s) 397, 503, 1034
HpyAV CCTTC 7 cut(s) 7, 17, 147, 536, 610, 699, 1075
HpyCH4III ACNGT 4 cut(s) 245, 270, 397, 872
HpyCH4V TGCA 6 cut(s) 37, 210, 302, 683, 715, 966
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 600, 721
HpyF3I CTNAG 2 cut(s) 145, 936
Hsp92I GRCGYC 1 cut(s) 1032
Hsp92II CATG 5 cut(s) 41, 76, 292, 652, 790
HspAI GCGC 3 cut(s) 364, 601, 790
KflI GGGWCCC 1 cut(s) 106
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
KspAI GTTAAC 1 cut(s) 442
Kzo9I GATC 6 cut(s) 40, 370, 495, 664, 799, 939
LmnI GCTCC 2 cut(s) 16, 128
Lsp1109I GCAGC 3 cut(s) 136, 142, 238
MaeI CTAG 2 cut(s) 194, 623
MaeIII GTNAC 2 cut(s) 950, 1052
MalI GATC 6 cut(s) 42, 372, 497, 666, 801, 941
MbiI CCGCTC 2 cut(s) 594, 845
MboI GATC 6 cut(s) 40, 370, 495, 664, 799, 939
MboII GAAGA 1 cut(s) 245
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 4 cut(s) 282, 567, 717, 918
MluCI AATT 3 cut(s) 340, 640, 706
MluI ACGCGT 1 cut(s) 823
MmeI TCCRAC 1 cut(s) 610
MnlI CCTC 9 cut(s) 457, 460, 514, 517, 543, 646, 876, 888, 941
MroNI GCCGGC 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 332
MseI TTAA 5 cut(s) 188, 231, 347, 441, 702
MslI CAYNNNNRTG 3 cut(s) 287, 395, 959
MspA1I CMGCKG 1 cut(s) 155
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 6 cut(s) 110, 141, 380, 507, 533, 1050
MspR9I CCNGG 2 cut(s) 110, 111
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 3 cut(s) 155, 600, 721
NaeI GCCGGC 1 cut(s) 381
NciI CCSGG 2 cut(s) 110, 111
NdeII GATC 6 cut(s) 40, 370, 495, 664, 799, 939
NgoMIV GCCGGC 1 cut(s) 379
NlaIII CATG 5 cut(s) 41, 76, 292, 652, 790
NlaIV GGNNCC 5 cut(s) 107, 108, 114, 565, 928
NmeAIII GCCGAG 2 cut(s) 385, 544
NmuCI GTSAC 1 cut(s) 950
OliI CACNNNNGTG 1 cut(s) 395
PaqCI CACCTGC 1 cut(s) 68
PcsI WCGNNNNNNNCGW 2 cut(s) 357, 822
PdiI GCCGGC 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 2 cut(s) 49, 855
PinAI ACCGGT 1 cut(s) 1049
PkrI GCNGC 7 cut(s) 151, 154, 157, 228, 596, 726, 863
PpuMI RGGWCCY 3 cut(s) 106, 134, 619
PsiI TTATAA 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 282
Psp5II RGGWCCY 3 cut(s) 106, 134, 619
PspFI CCCAGC 1 cut(s) 832
PspN4I GGNNCC 5 cut(s) 107, 108, 114, 565, 928
PspOMI GGGCCC 1 cut(s) 563
PspPI GGNCC 8 cut(s) 106, 113, 134, 201, 563, 564, 619, 875
PspPPI RGGWCCY 3 cut(s) 106, 134, 619
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 664
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 3 cut(s) 287, 395, 959
SacI GAGCTC 1 cut(s) 282
SaqAI TTAA 5 cut(s) 188, 231, 347, 441, 702
SatI GCNGC 7 cut(s) 150, 153, 156, 227, 595, 725, 862
Sau3AI GATC 6 cut(s) 40, 370, 495, 664, 799, 939
Sau96I GGNCC 8 cut(s) 106, 113, 134, 201, 563, 564, 619, 875
ScrFI CCNGG 2 cut(s) 110, 111
SduI GDGCHC 4 cut(s) 282, 567, 717, 918
SfcI CTRYAG 1 cut(s) 300
SinI GGWCC 4 cut(s) 106, 134, 201, 619
SmaI CCCGGG 1 cut(s) 111
SmiMI CAYNNNNRTG 3 cut(s) 287, 395, 959
SmlI CTYRAG 4 cut(s) 164, 230, 773, 887
SmoI CTYRAG 4 cut(s) 164, 230, 773, 887
Sse9I AATT 3 cut(s) 340, 640, 706
SsiI CCGC 6 cut(s) 153, 426, 594, 725, 843, 862
SspMI CTAG 2 cut(s) 194, 623
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 2 cut(s) 108, 109
TaaI ACNGT 4 cut(s) 245, 270, 397, 872
TaqI TCGA 6 cut(s) 89, 746, 761, 882, 918, 1029
TasI AATT 3 cut(s) 340, 640, 706
TauI GCSGC 4 cut(s) 155, 597, 727, 864
TfiI GAWTC 2 cut(s) 49, 855
Tru1I TTAA 5 cut(s) 188, 231, 347, 441, 702
Tru9I TTAA 5 cut(s) 188, 231, 347, 441, 702
TscAI CASTG 2 cut(s) 123, 494
TseFI GTSAC 1 cut(s) 950
TseI GCWGC 3 cut(s) 149, 155, 226
Tsp45I GTSAC 1 cut(s) 950
TspDTI ATGAA 2 cut(s) 41, 245
TspGWI ACGGA 1 cut(s) 239
TspMI CCCGGG 1 cut(s) 109
TspRI CASTG 2 cut(s) 123, 494
Vha464I CTTAAG 1 cut(s) 230
VneI GTGCAC 1 cut(s) 713
VpaK11BI GGWCC 4 cut(s) 106, 134, 201, 619
XapI RAATTY 2 cut(s) 640, 706
XcmI CCANNNNNNNNNTGG 2 cut(s) 70, 793
XmaI CCCGGG 1 cut(s) 109
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 2 cut(s) 194, 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.