MD02G1046700.v1.1

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010803
Physical Location & Seq
Reverse (-)
3719433 .. 3722141
2709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1046700.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGGACTTTTTGAAAGAATTGGAAGGAAAGCGAGTTCATGATCCTTTTGAAGCGAACATGGCAACTAAACCTACAGCAAAGCGCATATGGGTTCCGGGGCCGGTCATCGTCGGAGCTGGTCCTTCCGGCTTAGCAGTTGCTGCTTGTCTCAAAGAAAAAGGCATTCCAAGCTTGATCCTTGAAAGAGCTAATTGCATAGCTTCGTTATGGCAGCTCAAGACCTACGACCGTCTCCGGCTTCATCTCCCCAAGCAATTCTGTGAGCTCATACTCATGCCATTCCCGTCAGATTTTCCTACTTACCCAACAAAGCAGCAGTTTGTGGGTTACCTGAATGCTTATGCTGAAAATTTTGATTTGAAGCCGGCTTTCAACAGGACGGTGGTGAGTGCAAGGTATGATAGTAGATGTGGCCTTTGGTTTGTTAAGACTTTGGGATTGAAAAATGAGGAGACTGAGTATGTTTGTCAATGGCTGATTGTTGCCACTGGTGAAAATGCTGAGGAAGTTGTGCCAAAATTTGAAGGAGTGATGGTGAGTTGTTTCGGGGAAAGAAGGTTTTTGGTGGTTGGATGTGGTAATTCAGGCATGGAGGTCTGTTTGGATCTTTGCAACTACAATGCACGCCCTTCCCTAGTGGTAAAAAATTCGGTGCACATCTTGCCGCAAGAGATGCTTGGAATATCAACTTTTGGATTGTCCATGTGGTTACTCAAGCGGTTCCCCATGCGCTTTGTGGATCGTATTTTGCTTCTTGTCTCACGCTTCATGCTCGGCGACACTGCTCGGTTTGGAATCCACCGTCCTGAGCGTGGCCCTCTTGAGCTCAAGAGCATGACTGGCAAGACTCCTGTTTTGGACATTGGCACCCTTGCTAAGATCAAAACTGGCCACATCAAGGAAACGAGCATGTTTTCTGAAAAAGATGGGTTGCCTCGAAAGCCATTCCCGAACGGGTGGAAAGGGGAATGTGGGTTGTACTCAGTGGGGTTTACACAACGTGGCCTGCTGGGTGCATCTCTTGATGCAACGAGGATTGCAGAGGATATTGAGCATAGCTGGAAAGCTAAGGCCACGCATTTTATGGCTTTCACTACTTGTGCACCGCCACCGCCACCGCCACCACAACCTGCAACTTTCAATTAA

Protein Analysis

382

Amino Acids

42.55

Weight (kDa)

8.96

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_3 PF13738 35 - 217 2.4e-16 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 36 - 241 2.5e-10 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13260 AT4G13260 AT4G13260
fragaria_vesca FvH4_1g04800
malus_domestica MD01G1067600.v1.1 MD02G1046700.v1.1 MD15G1184800.v1.1
prunus_persica Prupe.7G231200_v2.0.a1
pyrus_communis pycom02g03910 pycom15g16630
rosa_chinensis RchiOBHm_Chr2g0090431
rosa_laevigata RLG00000016108
rosa_multiflora Rmu_sc0004514.1_g000016
rosa_roxburghii Rroxscaffold_2G00151160
rosa_rugosa Rorug02G0008000
rosa_samantha Rh2AG053400 Rh2BG052700 Rh2CG054800 Rh2DG053600
rosa_wichuraiana Rw2G004360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1138
AccB1I GGYRCC 1 cut(s) 866
AciI CCGC 5 cut(s) 665, 718, 1106, 1112, 1118
AclWI GGATC 4 cut(s) 35, 169, 612, 747
AcoI YGGCCR 1 cut(s) 889
AcsI RAATTY 3 cut(s) 349, 518, 646
AdeI CACNNNGTG 1 cut(s) 1001
AfaI GTAC 1 cut(s) 980
AfiI CCNNNNNNNGG 2 cut(s) 812, 898
AgsI TTSAA 8 cut(s) 13, 50, 182, 361, 373, 442, 524, 1141
AloI GAACNNNNNNTCC 2 cut(s) 18, 50
AluBI AGCT 9 cut(s) 116, 171, 188, 200, 214, 265, 826, 1059, 1067
AluI AGCT 9 cut(s) 116, 171, 188, 200, 214, 265, 826, 1059, 1067
Alw21I GWGCWC 4 cut(s) 267, 657, 828, 1105
Alw26I GTCTC 4 cut(s) 152, 236, 446, 763
Alw44I GTGCAC 2 cut(s) 653, 1101
AlwI GGATC 4 cut(s) 35, 169, 612, 747
AlwNI CAGNNNCTG 1 cut(s) 140
AoxI GGCC 6 cut(s) 98, 412, 814, 889, 1003, 1071
ApaLI GTGCAC 2 cut(s) 653, 1101
ApeKI GCWGC 3 cut(s) 140, 211, 313
ApoI RAATTY 3 cut(s) 349, 518, 646
ArsI GACNNNNNNTTYG 2 cut(s) 838, 870
AspLEI GCGC 2 cut(s) 84, 732
AspS9I GGNCC 3 cut(s) 98, 119, 815
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 3 cut(s) 397, 503, 547
AvaII GGWCC 1 cut(s) 119
BaeGI GKGCMC 2 cut(s) 657, 1105
BalI TGGCCA 1 cut(s) 891
BanI GGYRCC 1 cut(s) 866
BanII GRGCYC 2 cut(s) 267, 828
Bbv12I GWGCWC 4 cut(s) 267, 657, 828, 1105
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 3 cut(s) 127, 223, 325
BccI CCATC 2 cut(s) 526, 920
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 4 cut(s) 152, 236, 446, 763
BfaI CTAG 1 cut(s) 635
BfmI CTRYAG 1 cut(s) 72
BfuAI ACCTGC 1 cut(s) 1138
BisI GCNGC 4 cut(s) 141, 212, 314, 665
BlpI GCTNAGC 1 cut(s) 130
BlsI GCNGC 4 cut(s) 142, 213, 315, 666
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 1 cut(s) 119
BmgT120I GGNCC 3 cut(s) 98, 119, 815
BmiI GGNNCC 4 cut(s) 93, 99, 722, 868
BmrFI CCNGG 1 cut(s) 96
BmsI GCATC 3 cut(s) 663, 1015, 1025
Bpu10I CCTNAGC 3 cut(s) 501, 807, 1068
Bpu1102I GCTNAGC 1 cut(s) 130
BpuEI CTTGAG 4 cut(s) 200, 698, 812, 842
BpuMI CCSGG 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 95
BsaXI ACNNNNNCTCC 4 cut(s) 443, 473, 519, 549
Bsc4I CCNNNNNNNGG 2 cut(s) 812, 898
Bse118I RCCGGY 2 cut(s) 100, 364
Bse1I ACTGG 3 cut(s) 493, 844, 892
BseDI CCNNGG 1 cut(s) 95
BseGI GGATG 1 cut(s) 578
BseLI CCNNNNNNNGG 2 cut(s) 812, 898
BseMII CTCAG 4 cut(s) 447, 492, 798, 996
BseNI ACTGG 3 cut(s) 493, 844, 892
BseRI GAGGAG 1 cut(s) 464
BseSI GKGCMC 2 cut(s) 657, 1105
BseXI GCAGC 3 cut(s) 127, 223, 325
BseYI CCCAGC 1 cut(s) 1009
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 6 cut(s) 100, 414, 816, 891, 1005, 1073
BshNI GGYRCC 1 cut(s) 866
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 4 cut(s) 267, 657, 828, 1105
BsiSI CCGG 5 cut(s) 95, 101, 126, 235, 365
BslI CCNNNNNNNGG 2 cut(s) 812, 898
BsmAI GTCTC 4 cut(s) 152, 236, 446, 763
BsmBI CGTCTC 1 cut(s) 236
BsmI GAATGC 2 cut(s) 162, 340
BsnI GGCC 6 cut(s) 100, 414, 816, 891, 1005, 1073
Bsp1286I GDGCHC 4 cut(s) 267, 657, 828, 1105
Bsp143I GATC 5 cut(s) 40, 174, 604, 739, 879
Bsp1720I GCTNAGC 1 cut(s) 130
BspACI CCGC 5 cut(s) 665, 718, 1106, 1112, 1118
BspANI GGCC 6 cut(s) 100, 414, 816, 891, 1005, 1073
BspCNI CTCAG 4 cut(s) 448, 493, 799, 995
BspHI TCATGA 1 cut(s) 37
BspLI GGNNCC 4 cut(s) 93, 99, 722, 868
BspMI ACCTGC 1 cut(s) 1138
BspPI GGATC 4 cut(s) 35, 169, 612, 747
BspT107I GGYRCC 1 cut(s) 866
BsrFI RCCGGY 2 cut(s) 100, 364
BsrI ACTGG 3 cut(s) 493, 844, 892
BssAI RCCGGY 2 cut(s) 100, 364
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 5 cut(s) 40, 174, 604, 739, 879
Bst4CI ACNGT 3 cut(s) 230, 382, 803
BstAPI GCANNNNNTGC 3 cut(s) 140, 661, 673
BstC8I GCNNGC 3 cut(s) 366, 625, 1007
BstDEI CTNAG 7 cut(s) 130, 456, 501, 807, 876, 982, 1068
BstEII GGTNACC 1 cut(s) 326
BstF5I GGATG 1 cut(s) 578
BstHHI GCGC 2 cut(s) 84, 732
BstKTI GATC 5 cut(s) 43, 177, 607, 742, 882
BstMAI GTCTC 4 cut(s) 152, 236, 446, 763
BstMBI GATC 5 cut(s) 40, 174, 604, 739, 879
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 7 cut(s) 59, 140, 168, 661, 673, 840, 940
BstNSI RCATGY 1 cut(s) 913
BstPI GGTNACC 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 94
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 2 cut(s) 657, 1105
BstV1I GCAGC 3 cut(s) 127, 223, 325
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BsuRI GGCC 6 cut(s) 100, 414, 816, 891, 1005, 1073
BtsCI GGATG 1 cut(s) 578
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 3 cut(s) 486, 780, 990
BveI ACCTGC 1 cut(s) 1138
Cac8I GCNNGC 3 cut(s) 366, 625, 1007
CaiI CAGNNNCTG 1 cut(s) 140
CciI TCATGA 1 cut(s) 37
CfoI GCGC 2 cut(s) 84, 732
Cfr10I RCCGGY 2 cut(s) 100, 364
Cfr13I GGNCC 3 cut(s) 98, 119, 815
Csp6I GTAC 1 cut(s) 979
CviAII CATG 9 cut(s) 38, 58, 274, 589, 703, 727, 769, 835, 910
CviQI GTAC 1 cut(s) 979
DdeI CTNAG 7 cut(s) 130, 456, 501, 807, 876, 982, 1068
DpnI GATC 5 cut(s) 42, 176, 606, 741, 881
DpnII GATC 5 cut(s) 40, 174, 604, 739, 879
DraIII CACNNNGTG 1 cut(s) 1001
EaeI YGGCCR 1 cut(s) 889
Ecl136II GAGCTC 2 cut(s) 265, 826
Eco24I GRGCYC 2 cut(s) 267, 828
Eco47I GGWCC 1 cut(s) 119
Eco53kI GAGCTC 2 cut(s) 265, 826
Eco91I GGTNACC 1 cut(s) 326
EcoICRI GAGCTC 2 cut(s) 265, 826
EcoO65I GGTNACC 1 cut(s) 326
EcoT38I GRGCYC 2 cut(s) 267, 828
Esp3I CGTCTC 1 cut(s) 236
FaeI CATG 9 cut(s) 41, 61, 277, 592, 706, 730, 772, 838, 913
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FatI CATG 9 cut(s) 37, 57, 273, 588, 702, 726, 768, 834, 909
FauNDI CATATG 1 cut(s) 86
Fnu4HI GCNGC 4 cut(s) 141, 212, 314, 665
FokI GGATG 1 cut(s) 585
FriOI GRGCYC 2 cut(s) 267, 828
Fsp4HI GCNGC 4 cut(s) 141, 212, 314, 665
FspBI CTAG 1 cut(s) 635
GlaI GCGC 2 cut(s) 83, 731
GluI GCNGC 4 cut(s) 141, 212, 314, 665
GsaI CCCAGC 1 cut(s) 1013
HaeIII GGCC 6 cut(s) 100, 414, 816, 891, 1005, 1073
HapII CCGG 5 cut(s) 95, 101, 126, 235, 365
HhaI GCGC 2 cut(s) 84, 732
Hin1II CATG 9 cut(s) 41, 61, 277, 592, 706, 730, 772, 838, 913
Hin6I GCGC 2 cut(s) 82, 730
HinP1I GCGC 2 cut(s) 82, 730
HindIII AAGCTT 1 cut(s) 169
HinfI GANTC 2 cut(s) 795, 847
HpaII CCGG 5 cut(s) 95, 101, 126, 235, 365
HphI GGTGA 3 cut(s) 397, 503, 547
Hpy166II GTNNAC 3 cut(s) 655, 993, 1103
Hpy188I TCNGA 3 cut(s) 113, 289, 919
Hpy188III TCNNGA 7 cut(s) 38, 217, 806, 821, 829, 949, 1022
Hpy8I GTNNAC 3 cut(s) 655, 993, 1103
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 5 cut(s) 17, 132, 518, 549, 639
HpyCH4III ACNGT 3 cut(s) 230, 382, 803
HpyCH4IV ACGT 1 cut(s) 1000
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 140, 168, 661, 673, 840, 940
HpyF3I CTNAG 7 cut(s) 130, 456, 501, 807, 876, 982, 1068
HpySE526I ACGT 1 cut(s) 1000
Hsp92II CATG 9 cut(s) 41, 61, 277, 592, 706, 730, 772, 838, 913
HspAI GCGC 2 cut(s) 82, 730
KroI GCCGGC 1 cut(s) 364
KroNI GCCGGC 1 cut(s) 366
Kzo9I GATC 5 cut(s) 40, 174, 604, 739, 879
LmnI GCTCC 1 cut(s) 113
Lsp1109I GCAGC 3 cut(s) 127, 223, 325
LweI GCATC 3 cut(s) 663, 1015, 1025
MaeI CTAG 1 cut(s) 635
MaeII ACGT 1 cut(s) 1000
MaeIII GTNAC 2 cut(s) 326, 708
MalI GATC 5 cut(s) 42, 176, 606, 741, 881
MboI GATC 5 cut(s) 40, 174, 604, 739, 879
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 4 cut(s) 267, 657, 828, 1105
MlsI TGGCCA 1 cut(s) 891
MluCI AATT 8 cut(s) 17, 190, 254, 349, 518, 580, 646, 1141
MluNI TGGCCA 1 cut(s) 891
MlyI GAGTC 1 cut(s) 841
MmeI TCCRAC 2 cut(s) 91, 550
MnlI CCTC 7 cut(s) 442, 496, 586, 828, 945, 1026, 1036
Mox20I TGGCCA 1 cut(s) 891
MroNI GCCGGC 1 cut(s) 364
MscI TGGCCA 1 cut(s) 891
MseI TTAA 2 cut(s) 426, 1144
MslI CAYNNNNRTG 1 cut(s) 272
Msp20I TGGCCA 1 cut(s) 891
MspI CCGG 5 cut(s) 95, 101, 126, 235, 365
MspR9I CCNGG 1 cut(s) 96
Mva1269I GAATGC 2 cut(s) 162, 340
MwoI GCNNNNNNNGC 7 cut(s) 59, 140, 168, 661, 673, 840, 940
NaeI GCCGGC 1 cut(s) 366
NciI CCSGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 86
NdeII GATC 5 cut(s) 40, 174, 604, 739, 879
NgoMIV GCCGGC 1 cut(s) 364
NlaIII CATG 9 cut(s) 41, 61, 277, 592, 706, 730, 772, 838, 913
NlaIV GGNNCC 4 cut(s) 93, 99, 722, 868
NmeAIII GCCGAG 1 cut(s) 753
NspI RCATGY 1 cut(s) 913
PagI TCATGA 1 cut(s) 37
PctI GAATGC 2 cut(s) 162, 340
PdiI GCCGGC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 795
PkrI GCNGC 4 cut(s) 142, 213, 315, 666
PleI GAGTC 1 cut(s) 841
PpsI GAGTC 1 cut(s) 841
Psp124BI GAGCTC 2 cut(s) 267, 828
PspEI GGTNACC 1 cut(s) 326
PspFI CCCAGC 1 cut(s) 1009
PspN4I GGNNCC 4 cut(s) 93, 99, 722, 868
PspPI GGNCC 3 cut(s) 98, 119, 815
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 1 cut(s) 604
RsaI GTAC 1 cut(s) 980
RsaNI GTAC 1 cut(s) 979
RseI CAYNNNNRTG 1 cut(s) 272
SacI GAGCTC 2 cut(s) 267, 828
SaqAI TTAA 2 cut(s) 426, 1144
SatI GCNGC 4 cut(s) 141, 212, 314, 665
Sau3AI GATC 5 cut(s) 40, 174, 604, 739, 879
Sau96I GGNCC 3 cut(s) 98, 119, 815
SchI GAGTC 1 cut(s) 841
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 4 cut(s) 267, 657, 828, 1105
SfaNI GCATC 3 cut(s) 663, 1015, 1025
SfcI CTRYAG 1 cut(s) 72
SinI GGWCC 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 272
SmlI CTYRAG 4 cut(s) 215, 713, 821, 827
SmoI CTYRAG 4 cut(s) 215, 713, 821, 827
Sse9I AATT 8 cut(s) 17, 190, 254, 349, 518, 580, 646, 1141
SsiI CCGC 5 cut(s) 665, 718, 1106, 1112, 1118
SspMI CTAG 1 cut(s) 635
SstI GAGCTC 2 cut(s) 267, 828
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 3 cut(s) 230, 382, 803
TaiI ACGT 1 cut(s) 1003
TaqI TCGA 1 cut(s) 937
TasI AATT 8 cut(s) 17, 190, 254, 349, 518, 580, 646, 1141
TatI WGTACW 1 cut(s) 978
TauI GCSGC 1 cut(s) 667
TfiI GAWTC 1 cut(s) 795
Tru1I TTAA 2 cut(s) 426, 1144
Tru9I TTAA 2 cut(s) 426, 1144
TscAI CASTG 3 cut(s) 493, 787, 990
TseI GCWGC 3 cut(s) 140, 211, 313
TspDTI ATGAA 3 cut(s) 26, 230, 757
TspRI CASTG 3 cut(s) 493, 787, 990
VneI GTGCAC 2 cut(s) 653, 1101
VpaK11BI GGWCC 1 cut(s) 119
XapI RAATTY 3 cut(s) 349, 518, 646
XceI RCATGY 1 cut(s) 913
XcmI CCANNNNNNNNNTGG 2 cut(s) 733, 1081
XspI CTAG 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.