FvH4_1g04800

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010803
Physical Location & Seq
Reverse (-)
2532810 .. 2535865
3056 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04800.t1

Sequence Viewer

Length: 1314 bp
ATGGACTCTCTGAAGGAGCTAGAAGGCAAACGAGTACATGATCCTTTGATCCATTTCCAATCCAAAACAGACATGGCAGCTGCTAAAACGACGAGGCGTATATTGGTTCCGGGGCCGGTGATAGTCGGAGCAGGCCCTTCCGGCCTAGCCGCCGCAGCTTGTCTCAAGGAAAAAGGTATTCCGAGCTTAATCCTCGAAAGGGCTAACTGCATAGCTTCGTTGTGGCAGCTCAAGACGTACGACCGTCTCCGTCTTCATCTCCCTAAGAAGTTCTGCGAGCTCATTCTCATGCCATTCCCTGCAGATTTCCCAACCTACCCAACCAAGCAGCAGTTCCTCGGTTACTTAAAAGCTTATGCCGAGCGTTTTGATCTGAAGCCGGCTTTCAACACGACGGTGGTGAGTGCCAAGTACGACACTTGTTCCGGACTTTGGCTCGTCAAGACTTTGGGGTTGAAAAACGAGGAGGAGATTGAGTACGTTTGTCAGTGGCTAATCGTCGCCACCGGAGAAAATGCCGAGGAGGTTGTGCCGGAGTTTGAAGGAACGAAGGACTTTGGCGGGCCCATTGTTCATACCAGCTCTTACAAGAGCGGCGAGATGTTCCGGGAGAAGAAGGTTCTAGTTGTCGGATGTGGGAATTCAGGCATGGAGGTCTGCTTGGATCTCTGCAACTATAATGCAAAGCCTTCACTTGTTGTTAAAGATTCGGTGCACATCTTGCCGCAGGAGATACTGGGAATATCGACTTTTGGGCTGTCGATGTGGTTGCTCAAGTGGTTTCCCATGCGCCTTGTGGATCGCTTCTTGCTTCTCGTCTCACGCTTTCTGCTCGGCGACACCGCTCGCTTTGGAATCCACCGCCCTCGCCGTGGCCCTCTCGAGCTCAAGTCCGTCACTGGCAAGACACCTGTGCTTGATATTGGAACCCTCGCTAAGATCCGAAGTGGTCACATCAAGGTGTGCAAGGCGATTAAGCAAGTAAAACATCAGTCCGTTGAGTTCATAGACGGGAAAGTGGAGAGTTTCGACGCCATCATTTTGGCAACCGGTTACAGAAGTAATGTGATGTCTTGGTTGAAGGAAACGAGCATGTTTAGTGAGAAAAATGGATTGCCGAGGAAGGCATTTCCGAACGGGTGGAAAGGGGAGAGCGGGTTGTATTCTGTGGGGTTTACACAGCGTGGCCTGCTAGGAGCTTCAATGGATGCAAAGAGAATAGCAGAAGATATCGAGCATCGCAGGAAAGCTGAAAAGGCCACCCATATCATGCCCCGCTTGGTCACTTGTGCTTCTCCACTAGCACAACCCTAG

Protein Analysis

438

Amino Acids

48.66

Weight (kDa)

9.27

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_3 PF13738 40 - 237 8e-23 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_2 PF07992 40 - 235 2.7e-10 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 41 - 381 1.8e-30 Flavin-binding monooxygenase-like
Lys_Orn_oxgnase PF13434 105 - 231 3.2e-12 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13260 AT4G13260 AT4G13260
fragaria_vesca FvH4_1g04800
malus_domestica MD01G1067600.v1.1 MD02G1046700.v1.1 MD15G1184800.v1.1
prunus_persica Prupe.7G231200_v2.0.a1
pyrus_communis pycom02g03910 pycom15g16630
rosa_chinensis RchiOBHm_Chr2g0090431
rosa_laevigata RLG00000016108
rosa_multiflora Rmu_sc0004514.1_g000016
rosa_roxburghii Rroxscaffold_2G00151160
rosa_rugosa Rorug02G0008000
rosa_samantha Rh2AG053400 Rh2BG052700 Rh2CG054800 Rh2DG053600
rosa_wichuraiana Rw2G004360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 594, 845, 1155
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 9 cut(s) 150, 153, 561, 594, 725, 843, 862, 1155, 1276
AclWI GGATC 5 cut(s) 35, 43, 672, 807, 934
AcsI RAATTY 1 cut(s) 640
AcuI CTGAAG 2 cut(s) 32, 395
AcyI GRCGYC 1 cut(s) 1032
AdeI CACNNNGTG 1 cut(s) 1184
AfaI GTAC 4 cut(s) 36, 239, 413, 479
AfiI CCNNNNNNNGG 3 cut(s) 199, 432, 872
AgeI ACCGGT 1 cut(s) 1049
AgsI TTSAA 5 cut(s) 388, 457, 542, 1081, 1203
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AleI CACNNNNGTG 1 cut(s) 395
Alw21I GWGCWC 3 cut(s) 282, 717, 888
Alw26I GTCTC 3 cut(s) 167, 251, 823
Alw44I GTGCAC 1 cut(s) 713
AlwI GGATC 5 cut(s) 35, 43, 672, 807, 934
Ama87I CYCGRG 1 cut(s) 881
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 7 cut(s) 113, 133, 142, 563, 874, 1186, 1257
ApaI GGGCCC 1 cut(s) 567
ApaLI GTGCAC 1 cut(s) 713
ApeKI GCWGC 5 cut(s) 77, 80, 155, 226, 328
ApoI RAATTY 1 cut(s) 640
AsiGI ACCGGT 1 cut(s) 1049
AspLEI GCGC 1 cut(s) 792
AspS9I GGNCC 5 cut(s) 113, 134, 563, 564, 875
AsuC2I CCSGG 2 cut(s) 111, 608
AsuHPI GGTGA 2 cut(s) 130, 412
AvaI CYCGRG 1 cut(s) 881
BaeGI GKGCMC 2 cut(s) 567, 717
BanII GRGCYC 3 cut(s) 282, 567, 888
BbsI GAAGAC 1 cut(s) 245
Bbv12I GWGCWC 3 cut(s) 282, 717, 888
BbvI GCAGC 5 cut(s) 67, 89, 167, 238, 340
BccI CCATC 1 cut(s) 1043
BceAI ACGGC 1 cut(s) 855
BcgI CGANNNNNNTGC 2 cut(s) 751, 785
BcnI CCSGG 2 cut(s) 111, 608
BcoDI GTCTC 3 cut(s) 167, 251, 823
BfaI CTAG 6 cut(s) 20, 146, 623, 1193, 1301, 1312
BfmI CTRYAG 1 cut(s) 300
BglI GCCNNNNNGGC 1 cut(s) 141
BisI GCNGC 9 cut(s) 78, 81, 150, 153, 156, 227, 329, 595, 725
BlsI GCNGC 9 cut(s) 79, 82, 151, 154, 157, 228, 330, 596, 726
Bme1390I CCNGG 2 cut(s) 111, 608
BmeT110I CYCGRG 1 cut(s) 881
BmgT120I GGNCC 5 cut(s) 113, 134, 563, 564, 875
BmiI GGNNCC 4 cut(s) 108, 114, 565, 928
BmrFI CCNGG 2 cut(s) 111, 608
BmrI ACTGGG 1 cut(s) 746
BmsI GCATC 2 cut(s) 1198, 1246
BmuI ACTGGG 1 cut(s) 746
BpiI GAAGAC 1 cut(s) 245
BpuEI CTTGAG 4 cut(s) 149, 215, 758, 872
BpuMI CCSGG 2 cut(s) 111, 608
BsaHI GRCGYC 1 cut(s) 1032
BsaJI CCNNGG 5 cut(s) 110, 337, 519, 871, 1118
BsaWI WCCGGW 3 cut(s) 425, 506, 1049
BsaXI ACNNNNNCTCC 4 cut(s) 461, 491, 1142, 1172
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 432, 872
Bse118I RCCGGY 3 cut(s) 115, 379, 1049
Bse1I ACTGG 2 cut(s) 741, 904
BseAI TCCGGA 1 cut(s) 425
BseDI CCNNGG 5 cut(s) 110, 337, 519, 871, 1118
BseGI GGATG 2 cut(s) 638, 1213
BseLI CCNNNNNNNGG 3 cut(s) 199, 432, 872
BseNI ACTGG 2 cut(s) 741, 904
BseRI GAGGAG 3 cut(s) 479, 482, 536
BseSI GKGCMC 2 cut(s) 567, 717
BseXI GCAGC 5 cut(s) 67, 89, 167, 238, 340
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 7 cut(s) 115, 135, 144, 565, 876, 1188, 1259
BshTI ACCGGT 1 cut(s) 1049
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 3 cut(s) 282, 717, 888
BsiHKCI CYCGRG 1 cut(s) 881
BsiSI CCGG 9 cut(s) 110, 116, 141, 380, 426, 507, 533, 607, 1050
BsiWI CGTACG 1 cut(s) 237
BslI CCNNNNNNNGG 3 cut(s) 199, 432, 872
BsmAI GTCTC 3 cut(s) 167, 251, 823
BsmBI CGTCTC 2 cut(s) 251, 823
BsnI GGCC 7 cut(s) 115, 135, 144, 565, 876, 1188, 1259
BsoBI CYCGRG 1 cut(s) 881
Bsp120I GGGCCC 1 cut(s) 563
Bsp1286I GDGCHC 4 cut(s) 282, 567, 717, 888
Bsp13I TCCGGA 1 cut(s) 425
Bsp143I GATC 6 cut(s) 40, 48, 370, 664, 799, 939
BspACI CCGC 9 cut(s) 150, 153, 561, 594, 725, 843, 862, 1155, 1276
BspANI GGCC 7 cut(s) 115, 135, 144, 565, 876, 1188, 1259
BspEI TCCGGA 1 cut(s) 425
BspLI GGNNCC 4 cut(s) 108, 114, 565, 928
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 5 cut(s) 35, 43, 672, 807, 934
BsrBI CCGCTC 3 cut(s) 594, 845, 1155
BsrFI RCCGGY 3 cut(s) 115, 379, 1049
BsrI ACTGG 2 cut(s) 741, 904
BssAI RCCGGY 3 cut(s) 115, 379, 1049
BssECI CCNNGG 5 cut(s) 110, 337, 519, 871, 1118
BssMI GATC 6 cut(s) 40, 48, 370, 664, 799, 939
BssNI GRCGYC 1 cut(s) 1032
Bst4CI ACNGT 2 cut(s) 245, 397
BstACI GRCGYC 1 cut(s) 1032
BstAPI GCANNNNNTGC 1 cut(s) 721
BstC8I GCNNGC 6 cut(s) 133, 278, 381, 563, 847, 1190
BstDEI CTNAG 2 cut(s) 264, 936
BstDSI CCRYGG 1 cut(s) 871
BstF5I GGATG 2 cut(s) 638, 1213
BstHHI GCGC 1 cut(s) 792
BstKTI GATC 6 cut(s) 43, 51, 373, 667, 802, 942
BstMAI GTCTC 3 cut(s) 167, 251, 823
BstMBI GATC 6 cut(s) 40, 48, 370, 664, 799, 939
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 5 cut(s) 141, 155, 721, 1189, 1256
BstNSI RCATGY 1 cut(s) 1096
BstSCI CCNGG 2 cut(s) 109, 606
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 2 cut(s) 567, 717
BstV1I GCAGC 5 cut(s) 67, 89, 167, 238, 340
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 2 cut(s) 664, 939
BstXI CCANNNNNNTGG 1 cut(s) 1042
BstYI RGATCY 2 cut(s) 664, 939
BsuRI GGCC 7 cut(s) 115, 135, 144, 565, 876, 1188, 1259
BtgI CCRYGG 1 cut(s) 871
BtgZI GCGATG 1 cut(s) 1223
BtsCI GGATG 2 cut(s) 638, 1213
BtsIMutI CAGTG 2 cut(s) 494, 897
Cac8I GCNNGC 6 cut(s) 133, 278, 381, 563, 847, 1190
CfoI GCGC 1 cut(s) 792
Cfr10I RCCGGY 3 cut(s) 115, 379, 1049
Cfr13I GGNCC 5 cut(s) 113, 134, 563, 564, 875
CseI GACGC 1 cut(s) 1040
Csp6I GTAC 4 cut(s) 35, 238, 412, 478
CspAI ACCGGT 1 cut(s) 1049
CviAII CATG 7 cut(s) 38, 73, 289, 649, 787, 1093, 1270
CviQI GTAC 4 cut(s) 35, 238, 412, 478
DdeI CTNAG 2 cut(s) 264, 936
DpnI GATC 6 cut(s) 42, 50, 372, 666, 801, 941
DpnII GATC 6 cut(s) 40, 48, 370, 664, 799, 939
DraIII CACNNNGTG 1 cut(s) 1184
Ecl136II GAGCTC 2 cut(s) 280, 886
Eco24I GRGCYC 3 cut(s) 282, 567, 888
Eco32I GATATC 1 cut(s) 1231
Eco53kI GAGCTC 2 cut(s) 280, 886
Eco57I CTGAAG 2 cut(s) 32, 395
Eco88I CYCGRG 1 cut(s) 881
EcoICRI GAGCTC 2 cut(s) 280, 886
EcoO109I RGGNCCY 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 640
EcoRV GATATC 1 cut(s) 1231
EcoT38I GRGCYC 3 cut(s) 282, 567, 888
Esp3I CGTCTC 2 cut(s) 251, 823
FaeI CATG 7 cut(s) 41, 76, 292, 652, 790, 1096, 1273
FatI CATG 7 cut(s) 37, 72, 288, 648, 786, 1092, 1269
FauI CCCGC 3 cut(s) 554, 1148, 1283
Fnu4HI GCNGC 9 cut(s) 78, 81, 150, 153, 156, 227, 329, 595, 725
FokI GGATG 2 cut(s) 645, 1220
FriOI GRGCYC 3 cut(s) 282, 567, 888
Fsp4HI GCNGC 9 cut(s) 78, 81, 150, 153, 156, 227, 329, 595, 725
FspBI CTAG 6 cut(s) 20, 146, 623, 1193, 1301, 1312
GlaI GCGC 1 cut(s) 791
GluI GCNGC 9 cut(s) 78, 81, 150, 153, 156, 227, 329, 595, 725
HaeIII GGCC 7 cut(s) 115, 135, 144, 565, 876, 1188, 1259
HapII CCGG 9 cut(s) 110, 116, 141, 380, 426, 507, 533, 607, 1050
HgaI GACGC 1 cut(s) 1040
HhaI GCGC 1 cut(s) 792
Hin1I GRCGYC 1 cut(s) 1032
Hin1II CATG 7 cut(s) 41, 76, 292, 652, 790, 1096, 1273
Hin6I GCGC 1 cut(s) 790
HinP1I GCGC 1 cut(s) 790
HindIII AAGCTT 1 cut(s) 351
HinfI GANTC 3 cut(s) 5, 707, 855
HpaII CCGG 9 cut(s) 110, 116, 141, 380, 426, 507, 533, 607, 1050
HphI GGTGA 2 cut(s) 130, 412
Hpy166II GTNNAC 2 cut(s) 715, 1176
Hpy188I TCNGA 7 cut(s) 12, 128, 183, 375, 632, 944, 1134
Hpy188III TCNNGA 4 cut(s) 232, 426, 442, 881
Hpy8I GTNNAC 2 cut(s) 715, 1176
Hpy99I CGWCG 4 cut(s) 94, 397, 503, 1034
HpyAV CCTTC 9 cut(s) 7, 17, 147, 536, 544, 610, 699, 1075, 1117
HpyCH4III ACNGT 2 cut(s) 245, 397
HpyCH4IV ACGT 2 cut(s) 236, 480
HpyCH4V TGCA 7 cut(s) 210, 302, 672, 683, 715, 966, 1211
HpyF10VI GCNNNNNNNGC 5 cut(s) 141, 155, 721, 1189, 1256
HpyF3I CTNAG 2 cut(s) 264, 936
HpySE526I ACGT 2 cut(s) 236, 480
Hsp92I GRCGYC 1 cut(s) 1032
Hsp92II CATG 7 cut(s) 41, 76, 292, 652, 790, 1096, 1273
HspAI GCGC 1 cut(s) 790
Kpn2I TCCGGA 1 cut(s) 425
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
Kzo9I GATC 6 cut(s) 40, 48, 370, 664, 799, 939
LmnI GCTCC 3 cut(s) 16, 128, 1196
Lsp1109I GCAGC 5 cut(s) 67, 89, 167, 238, 340
LweI GCATC 2 cut(s) 1198, 1246
MaeI CTAG 6 cut(s) 20, 146, 623, 1193, 1301, 1312
MaeII ACGT 2 cut(s) 236, 480
MaeIII GTNAC 5 cut(s) 341, 895, 950, 1052, 1282
MalI GATC 6 cut(s) 42, 50, 372, 666, 801, 941
MbiI CCGCTC 3 cut(s) 594, 845, 1155
MboI GATC 6 cut(s) 40, 48, 370, 664, 799, 939
MboII GAAGA 3 cut(s) 245, 625, 1238
MflI RGATCY 2 cut(s) 664, 939
MhlI GDGCHC 4 cut(s) 282, 567, 717, 888
MluCI AATT 1 cut(s) 640
MmeI TCCRAC 2 cut(s) 106, 610
MroI TCCGGA 1 cut(s) 425
MroNI GCCGGC 1 cut(s) 379
MseI TTAA 4 cut(s) 188, 347, 702, 975
MslI CAYNNNNRTG 3 cut(s) 287, 395, 959
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 9 cut(s) 110, 116, 141, 380, 426, 507, 533, 607, 1050
MspR9I CCNGG 2 cut(s) 111, 608
MwoI GCNNNNNNNGC 5 cut(s) 141, 155, 721, 1189, 1256
NaeI GCCGGC 1 cut(s) 381
NciI CCSGG 2 cut(s) 111, 608
NdeII GATC 6 cut(s) 40, 48, 370, 664, 799, 939
NgoMIV GCCGGC 1 cut(s) 379
NlaIII CATG 7 cut(s) 41, 76, 292, 652, 790, 1096, 1273
NlaIV GGNNCC 4 cut(s) 108, 114, 565, 928
NmeAIII GCCGAG 4 cut(s) 385, 544, 813, 1143
NmuCI GTSAC 3 cut(s) 895, 950, 1282
NspI RCATGY 1 cut(s) 1096
OliI CACNNNNGTG 1 cut(s) 395
PaeR7I CTCGAG 1 cut(s) 881
PdiI GCCGGC 1 cut(s) 381
PfeI GAWTC 2 cut(s) 707, 855
Pfl23II CGTACG 1 cut(s) 237
PfoI TCCNGGA 1 cut(s) 606
PinAI ACCGGT 1 cut(s) 1049
PkrI GCNGC 9 cut(s) 79, 82, 151, 154, 157, 228, 330, 596, 726
Psp124BI GAGCTC 2 cut(s) 282, 888
PspLI CGTACG 1 cut(s) 237
PspN4I GGNNCC 4 cut(s) 108, 114, 565, 928
PspOMI GGGCCC 1 cut(s) 563
PspPI GGNCC 5 cut(s) 113, 134, 563, 564, 875
PstI CTGCAG 1 cut(s) 304
PsuI RGATCY 2 cut(s) 664, 939
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 4 cut(s) 36, 239, 413, 479
RsaNI GTAC 4 cut(s) 35, 238, 412, 478
RseI CAYNNNNRTG 3 cut(s) 287, 395, 959
SacI GAGCTC 2 cut(s) 282, 888
SaqAI TTAA 4 cut(s) 188, 347, 702, 975
SatI GCNGC 9 cut(s) 78, 81, 150, 153, 156, 227, 329, 595, 725
Sau3AI GATC 6 cut(s) 40, 48, 370, 664, 799, 939
Sau96I GGNCC 5 cut(s) 113, 134, 563, 564, 875
ScrFI CCNGG 2 cut(s) 111, 608
SduI GDGCHC 4 cut(s) 282, 567, 717, 888
SfaNI GCATC 2 cut(s) 1198, 1246
SfcI CTRYAG 1 cut(s) 300
SfiI GGCCNNNNNGGCC 1 cut(s) 141
Sfr274I CTCGAG 1 cut(s) 881
SlaI CTCGAG 1 cut(s) 881
SmiMI CAYNNNNRTG 3 cut(s) 287, 395, 959
SmlI CTYRAG 5 cut(s) 164, 230, 773, 881, 887
SmoI CTYRAG 5 cut(s) 164, 230, 773, 881, 887
Sse9I AATT 1 cut(s) 640
SsiI CCGC 9 cut(s) 150, 153, 561, 594, 725, 843, 862, 1155, 1276
SspMI CTAG 6 cut(s) 20, 146, 623, 1193, 1301, 1312
SstI GAGCTC 2 cut(s) 282, 888
StyD4I CCNGG 2 cut(s) 109, 606
TaaI ACNGT 2 cut(s) 245, 397
TaiI ACGT 2 cut(s) 239, 483
TaqI TCGA 6 cut(s) 195, 746, 761, 882, 1029, 1233
TasI AATT 1 cut(s) 640
TatI WGTACW 1 cut(s) 34
TauI GCSGC 4 cut(s) 152, 155, 597, 727
TfiI GAWTC 2 cut(s) 707, 855
Tru1I TTAA 4 cut(s) 188, 347, 702, 975
Tru9I TTAA 4 cut(s) 188, 347, 702, 975
TscAI CASTG 2 cut(s) 494, 904
TseFI GTSAC 3 cut(s) 895, 950, 1282
TseI GCWGC 5 cut(s) 77, 80, 155, 226, 328
Tsp45I GTSAC 3 cut(s) 895, 950, 1282
TspDTI ATGAA 3 cut(s) 245, 563, 994
TspGWI ACGGA 3 cut(s) 239, 883, 985
TspRI CASTG 2 cut(s) 494, 904
VneI GTGCAC 1 cut(s) 713
XapI RAATTY 1 cut(s) 640
XceI RCATGY 1 cut(s) 1096
XcmI CCANNNNNNNNNTGG 2 cut(s) 70, 793
XhoI CTCGAG 1 cut(s) 881
XspI CTAG 6 cut(s) 20, 146, 623, 1193, 1301, 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.