Rh2DG053600

monooxygenase

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
Physical Location & Seq
Reverse (-)
3864917 .. 3868162
3246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG053600.1

Sequence Viewer

Length: 1110 bp
ATGGACTTTCTGAAGGAGCTGGAAGGCAAACGAATGCATGATCCTTTGATTCATTTCCAATCCAAAACAGACATGGCAGATGCTAAATCGACAAGGCGTATATGGGTCCCGGGGCCAGTGATAGTGGGAGCAGGTCCTTCCGGCTTAGCTGCCGCGGCTTGTCTCAAGGAAAAAGGTATTCCGAGCTTAATCCTAGAAAGGACCAACTGCATAGCTTCGTTGTGGCAGCTTAAGACCTACGACCGTCTCCGTCTTCATCTCCCAAAACAGTTCTGCGAGCTCATACTCATGCCATTCCCTGCAGATTTCCCAACCTACCCAACCAAGAAGCAGTTCGTGAATTACTTAAACGCTTATGCCGAGCGCTTTGATCTAAAGCCGGCTTTCAACACGACGGTGGTGAGTGCCAAGTACGATAGTGGTTACGGGCTTTGGTTGGTTAAGACTTTGGGGTTGAAAAATGAAGAGGCGATTGAGTATGTTTGTCAGTGGCTGATCGTCGCCACCGGAGAAAATGCCGAGGAGGTTGTGCCGGAGTTTGAAGGAGTGAGGGACTTTGGTGGGCCCATTGTCCATACCAGCTCTTACAAGAGCGGCGAGCGCTTCCGGGGGCAGAAGGTCCTAGTGGTCGGATGTGGGAATTCAGGCATGGAGGTTTGCTTGGATCTCTGCGGTTATAATGCAAAGCCTTCCCTTGTGGTTAAAAATTCGGTGCACATCTTGCCGCAAGAGATACTGGGAATATCGACTTTTGGGCTGTCGATGTGGTTGCTCAAGTGGTTTCCCATGCGCCTTGTGGATCGCTTCTTGCTTCTCGTCTCACGCGTTATGCTGGGCGACACCGCTCGCTTTGGAATCAGCCGCCCTCACCGTGGCCCTCTCGAACTCAAGTCCATCTCTGGCAAGACACCTGTGCTCGATGTTGGCACCCTCGCTAAGATCAGAAGTGGTCACATCAAGGTGTGCAAGGCAATCAAGCAAGTAAAACATCAATCAGTAGAGTTCATAGACGGGAAAGTGGAGTGTTTCGACGCCATTATTTTGGCAACCGGTTACAGAAGTAATGTGATGTCTTCATGGTTGAAGGTTGATATTGATGACTTGCTTTGA

Protein Analysis

369

Amino Acids

41.21

Weight (kDa)

8.92

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_3 PF13738 40 - 237 9.4e-23 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_2 PF07992 40 - 233 2.5e-10 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 41 - 353 2.7e-28 Flavin-binding monooxygenase-like
Lys_Orn_oxgnase PF13434 105 - 230 1.3e-11 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

Orthologous Genes (Group: OG0010803)

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
Acc36I ACCTGC 1 cut(s) 122
AccB1I GGYRCC 1 cut(s) 926
AccBSI CCGCTC 2 cut(s) 594, 845
AccII CGCG 2 cut(s) 155, 825
AciI CCGC 7 cut(s) 153, 155, 594, 672, 725, 843, 862
AclWI GGATC 3 cut(s) 35, 672, 807
AcsI RAATTY 2 cut(s) 640, 706
AcuI CTGAAG 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 1032
AfaI GTAC 1 cut(s) 413
AfeI AGCGCT 2 cut(s) 365, 602
AfiI CCNNNNNNNGG 1 cut(s) 872
AflII CTTAAG 1 cut(s) 230
AflIII ACRYGT 1 cut(s) 823
AgeI ACCGGT 1 cut(s) 1049
AgsI TTSAA 4 cut(s) 388, 457, 542, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AleI CACNNNNGTG 1 cut(s) 395
AluBI AGCT 7 cut(s) 19, 149, 186, 215, 229, 280, 582
AluI AGCT 7 cut(s) 19, 149, 186, 215, 229, 280, 582
Alw21I GWGCWC 3 cut(s) 282, 717, 918
Alw26I GTCTC 3 cut(s) 167, 251, 823
Alw44I GTGCAC 1 cut(s) 713
AlwI GGATC 3 cut(s) 35, 672, 807
AlwNI CAGNNNCTG 1 cut(s) 493
Ama87I CYCGRG 1 cut(s) 109
Aor51HI AGCGCT 2 cut(s) 365, 602
AoxI GGCC 3 cut(s) 113, 563, 874
ApaI GGGCCC 1 cut(s) 567
ApaLI GTGCAC 1 cut(s) 713
ApeKI GCWGC 2 cut(s) 149, 226
ApoI RAATTY 2 cut(s) 640, 706
AsiGI ACCGGT 1 cut(s) 1049
Asp700I GAANNNNTTC 1 cut(s) 332
AspLEI GCGC 3 cut(s) 366, 603, 792
AspS9I GGNCC 8 cut(s) 106, 113, 134, 201, 563, 564, 619, 875
AsuC2I CCSGG 3 cut(s) 110, 111, 608
AsuHPI GGTGA 2 cut(s) 412, 860
AvaI CYCGRG 1 cut(s) 109
AvaII GGWCC 4 cut(s) 106, 134, 201, 619
BaeGI GKGCMC 2 cut(s) 567, 717
BanI GGYRCC 1 cut(s) 926
BanII GRGCYC 2 cut(s) 282, 567
BbsI GAAGAC 2 cut(s) 245, 1065
Bbv12I GWGCWC 3 cut(s) 282, 717, 918
BbvI GCAGC 2 cut(s) 136, 238
BccI CCATC 1 cut(s) 902
BcgI CGANNNNNNTGC 2 cut(s) 751, 785
BcnI CCSGG 3 cut(s) 110, 111, 608
BcoDI GTCTC 3 cut(s) 167, 251, 823
BfaI CTAG 2 cut(s) 194, 623
BfmI CTRYAG 1 cut(s) 300
BfoI RGCGCY 2 cut(s) 367, 604
BfrI CTTAAG 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 122
BisI GCNGC 7 cut(s) 150, 153, 156, 227, 595, 725, 862
BlpI GCTNAGC 1 cut(s) 145
BlsI GCNGC 7 cut(s) 151, 154, 157, 228, 596, 726, 863
Bme1390I CCNGG 3 cut(s) 110, 111, 608
Bme18I GGWCC 4 cut(s) 106, 134, 201, 619
BmeT110I CYCGRG 1 cut(s) 109
BmgT120I GGNCC 8 cut(s) 106, 113, 134, 201, 563, 564, 619, 875
BmiI GGNNCC 5 cut(s) 107, 108, 114, 565, 928
BmrFI CCNGG 3 cut(s) 110, 111, 608
BmrI ACTGGG 1 cut(s) 746
BmsI GCATC 1 cut(s) 70
BmuI ACTGGG 1 cut(s) 746
BpiI GAAGAC 2 cut(s) 245, 1065
Bpu1102I GCTNAGC 1 cut(s) 145
BpuEI CTTGAG 3 cut(s) 149, 758, 872
BpuMI CCSGG 3 cut(s) 110, 111, 608
BsaHI GRCGYC 1 cut(s) 1032
BsaJI CCNNGG 6 cut(s) 109, 110, 153, 519, 607, 871
BsaWI WCCGGW 2 cut(s) 506, 1049
Bsc4I CCNNNNNNNGG 1 cut(s) 872
Bse118I RCCGGY 2 cut(s) 379, 1049
Bse1I ACTGG 2 cut(s) 116, 741
BseDI CCNNGG 6 cut(s) 109, 110, 153, 519, 607, 871
BseGI GGATG 1 cut(s) 638
BseLI CCNNNNNNNGG 1 cut(s) 872
BseNI ACTGG 2 cut(s) 116, 741
BseRI GAGGAG 1 cut(s) 536
BseSI GKGCMC 2 cut(s) 567, 717
BseXI GCAGC 2 cut(s) 136, 238
BseYI CCCAGC 1 cut(s) 832
Bsh1236I CGCG 2 cut(s) 155, 825
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 3 cut(s) 115, 565, 876
BshNI GGYRCC 1 cut(s) 926
BshTI ACCGGT 1 cut(s) 1049
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 3 cut(s) 282, 717, 918
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 7 cut(s) 110, 141, 380, 507, 533, 607, 1050
BslFI GGGAC 2 cut(s) 92, 566
BslI CCNNNNNNNGG 1 cut(s) 872
BsmAI GTCTC 3 cut(s) 167, 251, 823
BsmBI CGTCTC 2 cut(s) 251, 823
BsmFI GGGAC 2 cut(s) 92, 566
BsmI GAATGC 1 cut(s) 39
BsnI GGCC 3 cut(s) 115, 565, 876
BsoBI CYCGRG 1 cut(s) 109
Bsp120I GGGCCC 1 cut(s) 563
Bsp1286I GDGCHC 4 cut(s) 282, 567, 717, 918
Bsp143I GATC 6 cut(s) 40, 370, 495, 664, 799, 939
Bsp1720I GCTNAGC 1 cut(s) 145
BspACI CCGC 7 cut(s) 153, 155, 594, 672, 725, 843, 862
BspANI GGCC 3 cut(s) 115, 565, 876
BspFNI CGCG 2 cut(s) 155, 825
BspLI GGNNCC 5 cut(s) 107, 108, 114, 565, 928
BspMAI CTGCAG 1 cut(s) 304
BspMI ACCTGC 1 cut(s) 122
BspPI GGATC 3 cut(s) 35, 672, 807
BspT107I GGYRCC 1 cut(s) 926
BspTI CTTAAG 1 cut(s) 230
BsrBI CCGCTC 2 cut(s) 594, 845
BsrFI RCCGGY 2 cut(s) 379, 1049
BsrI ACTGG 2 cut(s) 116, 741
BssAI RCCGGY 2 cut(s) 379, 1049
BssECI CCNNGG 6 cut(s) 109, 110, 153, 519, 607, 871
BssMI GATC 6 cut(s) 40, 370, 495, 664, 799, 939
BssNI GRCGYC 1 cut(s) 1032
Bst4CI ACNGT 4 cut(s) 245, 270, 397, 872
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 1 cut(s) 1032
BstAFI CTTAAG 1 cut(s) 230
BstAPI GCANNNNNTGC 1 cut(s) 721
BstC8I GCNNGC 4 cut(s) 278, 381, 599, 847
BstDEI CTNAG 2 cut(s) 145, 936
BstDSI CCRYGG 2 cut(s) 153, 871
BstF5I GGATG 1 cut(s) 638
BstFNI CGCG 2 cut(s) 155, 825
BstH2I RGCGCY 2 cut(s) 367, 604
BstHHI GCGC 3 cut(s) 366, 603, 792
BstKTI GATC 6 cut(s) 43, 373, 498, 667, 802, 942
BstMAI GTCTC 3 cut(s) 167, 251, 823
BstMBI GATC 6 cut(s) 40, 370, 495, 664, 799, 939
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 3 cut(s) 155, 600, 721
BstSCI CCNGG 3 cut(s) 108, 109, 606
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 2 cut(s) 567, 717
BstUI CGCG 2 cut(s) 155, 825
BstV1I GCAGC 2 cut(s) 136, 238
BstV2I GAAGAC 2 cut(s) 245, 1065
BstX2I RGATCY 1 cut(s) 664
BstXI CCANNNNNNTGG 1 cut(s) 1042
BstYI RGATCY 1 cut(s) 664
BsuRI GGCC 3 cut(s) 115, 565, 876
BtgI CCRYGG 2 cut(s) 153, 871
BtsCI GGATG 1 cut(s) 638
BtsIMutI CAGTG 2 cut(s) 123, 494
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 4 cut(s) 278, 381, 599, 847
CaiI CAGNNNCTG 1 cut(s) 493
CfoI GCGC 3 cut(s) 366, 603, 792
Cfr10I RCCGGY 2 cut(s) 379, 1049
Cfr13I GGNCC 8 cut(s) 106, 113, 134, 201, 563, 564, 619, 875
Cfr42I CCGCGG 1 cut(s) 156
Cfr9I CCCGGG 1 cut(s) 109
CseI GACGC 1 cut(s) 1040
Csp6I GTAC 1 cut(s) 412
CspAI ACCGGT 1 cut(s) 1049
CviAII CATG 6 cut(s) 38, 73, 289, 649, 787, 1077
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 2 cut(s) 145, 936
DpnI GATC 6 cut(s) 42, 372, 497, 666, 801, 941
DpnII GATC 6 cut(s) 40, 370, 495, 664, 799, 939
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Ecl136II GAGCTC 1 cut(s) 280
Eco24I GRGCYC 2 cut(s) 282, 567
Eco47I GGWCC 4 cut(s) 106, 134, 201, 619
Eco47III AGCGCT 2 cut(s) 365, 602
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 3 cut(s) 106, 134, 619
EcoRI GAATTC 1 cut(s) 640
EcoT22I ATGCAT 1 cut(s) 39
EcoT38I GRGCYC 2 cut(s) 282, 567
Esp3I CGTCTC 2 cut(s) 251, 823
FaeI CATG 6 cut(s) 41, 76, 292, 652, 790, 1080
FaqI GGGAC 2 cut(s) 92, 566
FatI CATG 6 cut(s) 37, 72, 288, 648, 786, 1076
Fnu4HI GCNGC 7 cut(s) 150, 153, 156, 227, 595, 725, 862
FokI GGATG 1 cut(s) 645
FriOI GRGCYC 2 cut(s) 282, 567
Fsp4HI GCNGC 7 cut(s) 150, 153, 156, 227, 595, 725, 862
FspBI CTAG 2 cut(s) 194, 623
GlaI GCGC 3 cut(s) 365, 602, 791
GluI GCNGC 7 cut(s) 150, 153, 156, 227, 595, 725, 862
GsaI CCCAGC 1 cut(s) 836
HaeII RGCGCY 2 cut(s) 367, 604
HaeIII GGCC 3 cut(s) 115, 565, 876
HapII CCGG 7 cut(s) 110, 141, 380, 507, 533, 607, 1050
HgaI GACGC 1 cut(s) 1040
HhaI GCGC 3 cut(s) 366, 603, 792
Hin1I GRCGYC 1 cut(s) 1032
Hin1II CATG 6 cut(s) 41, 76, 292, 652, 790, 1080
Hin6I GCGC 3 cut(s) 364, 601, 790
HinP1I GCGC 3 cut(s) 364, 601, 790
HinfI GANTC 2 cut(s) 49, 855
HpaII CCGG 7 cut(s) 110, 141, 380, 507, 533, 607, 1050
HphI GGTGA 2 cut(s) 412, 860
Hpy166II GTNNAC 1 cut(s) 715
Hpy188I TCNGA 4 cut(s) 12, 183, 632, 944
Hpy188III TCNNGA 2 cut(s) 337, 881
Hpy8I GTNNAC 1 cut(s) 715
Hpy99I CGWCG 3 cut(s) 397, 503, 1034
HpyAV CCTTC 7 cut(s) 7, 17, 147, 536, 610, 699, 1078
HpyCH4III ACNGT 4 cut(s) 245, 270, 397, 872
HpyCH4V TGCA 6 cut(s) 37, 210, 302, 683, 715, 966
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 600, 721
HpyF3I CTNAG 2 cut(s) 145, 936
Hsp92I GRCGYC 1 cut(s) 1032
Hsp92II CATG 6 cut(s) 41, 76, 292, 652, 790, 1080
HspAI GCGC 3 cut(s) 364, 601, 790
KflI GGGWCCC 1 cut(s) 106
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 6 cut(s) 40, 370, 495, 664, 799, 939
LmnI GCTCC 2 cut(s) 16, 128
Lsp1109I GCAGC 2 cut(s) 136, 238
LweI GCATC 1 cut(s) 70
MaeI CTAG 2 cut(s) 194, 623
MaeIII GTNAC 3 cut(s) 422, 950, 1052
MalI GATC 6 cut(s) 42, 372, 497, 666, 801, 941
MbiI CCGCTC 2 cut(s) 594, 845
MboI GATC 6 cut(s) 40, 370, 495, 664, 799, 939
MboII GAAGA 3 cut(s) 245, 476, 1065
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 4 cut(s) 282, 567, 717, 918
MluCI AATT 3 cut(s) 340, 640, 706
MluI ACGCGT 1 cut(s) 823
MmeI TCCRAC 1 cut(s) 610
MnlI CCTC 8 cut(s) 460, 514, 517, 543, 646, 876, 888, 941
Mph1103I ATGCAT 1 cut(s) 39
MroNI GCCGGC 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 332
MseI TTAA 5 cut(s) 188, 231, 347, 441, 702
MslI CAYNNNNRTG 3 cut(s) 287, 395, 959
MspA1I CMGCKG 1 cut(s) 155
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 7 cut(s) 110, 141, 380, 507, 533, 607, 1050
MspR9I CCNGG 3 cut(s) 110, 111, 608
Mva1269I GAATGC 1 cut(s) 39
MvnI CGCG 2 cut(s) 155, 825
MwoI GCNNNNNNNGC 3 cut(s) 155, 600, 721
NaeI GCCGGC 1 cut(s) 381
NciI CCSGG 3 cut(s) 110, 111, 608
NdeII GATC 6 cut(s) 40, 370, 495, 664, 799, 939
NgoMIV GCCGGC 1 cut(s) 379
NlaIII CATG 6 cut(s) 41, 76, 292, 652, 790, 1080
NlaIV GGNNCC 5 cut(s) 107, 108, 114, 565, 928
NmeAIII GCCGAG 2 cut(s) 385, 544
NmuCI GTSAC 1 cut(s) 950
NsiI ATGCAT 1 cut(s) 39
OliI CACNNNNGTG 1 cut(s) 395
PcsI WCGNNNNNNNCGW 2 cut(s) 357, 822
PctI GAATGC 1 cut(s) 39
PdiI GCCGGC 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 2 cut(s) 49, 855
PinAI ACCGGT 1 cut(s) 1049
PkrI GCNGC 7 cut(s) 151, 154, 157, 228, 596, 726, 863
PpuMI RGGWCCY 3 cut(s) 106, 134, 619
PsiI TTATAA 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 282
Psp5II RGGWCCY 3 cut(s) 106, 134, 619
PspFI CCCAGC 1 cut(s) 832
PspN4I GGNNCC 5 cut(s) 107, 108, 114, 565, 928
PspOMI GGGCCC 1 cut(s) 563
PspPI GGNCC 8 cut(s) 106, 113, 134, 201, 563, 564, 619, 875
PspPPI RGGWCCY 3 cut(s) 106, 134, 619
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 664
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 3 cut(s) 287, 395, 959
SacI GAGCTC 1 cut(s) 282
SacII CCGCGG 1 cut(s) 156
SaqAI TTAA 5 cut(s) 188, 231, 347, 441, 702
SatI GCNGC 7 cut(s) 150, 153, 156, 227, 595, 725, 862
Sau3AI GATC 6 cut(s) 40, 370, 495, 664, 799, 939
Sau96I GGNCC 8 cut(s) 106, 113, 134, 201, 563, 564, 619, 875
ScrFI CCNGG 3 cut(s) 110, 111, 608
SduI GDGCHC 4 cut(s) 282, 567, 717, 918
SfaNI GCATC 1 cut(s) 70
SfcI CTRYAG 1 cut(s) 300
Sfr303I CCGCGG 1 cut(s) 156
SgrBI CCGCGG 1 cut(s) 156
SinI GGWCC 4 cut(s) 106, 134, 201, 619
SmaI CCCGGG 1 cut(s) 111
SmiMI CAYNNNNRTG 3 cut(s) 287, 395, 959
SmlI CTYRAG 4 cut(s) 164, 230, 773, 887
SmoI CTYRAG 4 cut(s) 164, 230, 773, 887
Sse9I AATT 3 cut(s) 340, 640, 706
SsiI CCGC 7 cut(s) 153, 155, 594, 672, 725, 843, 862
SspMI CTAG 2 cut(s) 194, 623
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 3 cut(s) 108, 109, 606
TaaI ACNGT 4 cut(s) 245, 270, 397, 872
TaqI TCGA 6 cut(s) 89, 746, 761, 882, 918, 1029
TasI AATT 3 cut(s) 340, 640, 706
TauI GCSGC 5 cut(s) 155, 158, 597, 727, 864
TfiI GAWTC 2 cut(s) 49, 855
Tru1I TTAA 5 cut(s) 188, 231, 347, 441, 702
Tru9I TTAA 5 cut(s) 188, 231, 347, 441, 702
TscAI CASTG 2 cut(s) 123, 494
TseFI GTSAC 1 cut(s) 950
TseI GCWGC 2 cut(s) 149, 226
Tsp45I GTSAC 1 cut(s) 950
TspDTI ATGAA 5 cut(s) 41, 245, 477, 994, 1065
TspGWI ACGGA 1 cut(s) 239
TspMI CCCGGG 1 cut(s) 109
TspRI CASTG 2 cut(s) 123, 494
Vha464I CTTAAG 1 cut(s) 230
VneI GTGCAC 1 cut(s) 713
VpaK11BI GGWCC 4 cut(s) 106, 134, 201, 619
XapI RAATTY 2 cut(s) 640, 706
XcmI CCANNNNNNNNNTGG 2 cut(s) 70, 793
XmaI CCCGGG 1 cut(s) 109
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 2 cut(s) 194, 623
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.