MD01G1067600.v1.1

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010803
Physical Location & Seq
Reverse (-)
17093902 .. 17096602
2701 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1067600.v1.1.491

Sequence Viewer

Length: 1311 bp
ATGGACTTTTTGAAAGAATTGGAAGGAAAGCGAGTTCATGATCCTTTTGAAGCGAACATGGCAACTAAACCTACAGCAAAGCGCATATGGGTTCCGGGGCCGGTCATCGTCGGAGCTGGTCCTTCCGGCTTAGCAGTTGCTGCTTGTCTCAAAGAAAAAGGCATTCCAAGCTTGATCCTTGAAAGAGCTAATTGCATAGCTTCGTTATGGCAGCTCAAGACCTACGACCGTCTCCGGCTTCATCTCCCCAAGCAATTCTGTGAGCTCATACTCATGCCATTCCCGTCAGATTTTCCTACTTACCCAACAAAGCAGCAGTTTGTGGGTTACCTGAATGCTTATGCTGAAAATTTTGATTTGAAGCCGGCTTTCAACAGGACGGTGGTGAGTGCAAGGTATGATAGTAGATGTGGCCTTTGGTTTGTTAAGACTTTGGGATTGAAAAATGAGGAGACTGAGTATGTTTGTCAATGGCTGATTGTTGCCACTGGTGAAAATGCTGAGGAAGTTGTGCCAAAATTTGAAGGAGTGAGTGAGTTTGGTGGGCCTATTGTCCACACTAGCTCTTACAAGAGTGGTGAGTTGTTTCGGGGAAAGAAGGTTTTGGTGGTTGGATGTGGTAATTCAGGCATGGAGGTCTGTTTGGATCTTTGCAACTACAATGCACGCCCTTCCCTAGTGGTAAAAAATTCGGTGCACATCTTGCCGCAAGAGATGCTTGGAATATCAACTTTTGGATTGTCCATGTGGTTACTCAAGCGGTTCCCCATGCGCTTTGTGGATCGTATTTTGCTTCTTGTCTCACGCTTCATGCTCGGCGACACTGCTCGGTTTGGAATCCACCGTCCTGAGCGTGGCCCTCTTGAGCTCAAGAGCATGACTGGCAAGACTCCTGTTTTGGACATTGGCACCCTTGCTAAGATCAAAACTGGCCACATCAAGGTTTGCACGGCAGTAAAACAACTAAAGCATCAAGCTGTGGAGTTTATAGATGGAAGAGTAGAGAATTTCGATGCTATCATTTATGCAACGGGGTACAGAAGCAATGTAATGTCTTGGTTGAAGGAAACGAGCATGTTTTCTGAAAAAGATGGGTTGCCTCGAAAGCCATTCCCGAACGGGTGGAAAGGGGAATGTGGGTTGTACTCAGTGGGGTTTACACAACGTGGCCTGCTGGGTGCATCTCTTGATGCAACGAGGATTGCAGAGGATATTGAGCATAGCTGGAAAGCTAAGGCCACGCATTTTATGGCTTTCACTACTTGTGCACCGCCACCGCCACCGCCACCACAACCTGCAACTTTCAATTAA

Protein Analysis

437

Amino Acids

48.61

Weight (kDa)

9.05

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_3 PF13738 35 - 231 9.4e-22 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_2 PF07992 35 - 231 1.1e-09 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 36 - 357 7.4e-32 Flavin-binding monooxygenase-like
Lys_Orn_oxgnase PF13434 100 - 223 9.6e-12 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13260 AT4G13260 AT4G13260
fragaria_vesca FvH4_1g04800
malus_domestica MD01G1067600.v1.1 MD02G1046700.v1.1 MD15G1184800.v1.1
prunus_persica Prupe.7G231200_v2.0.a1
pyrus_communis pycom02g03910 pycom15g16630
rosa_chinensis RchiOBHm_Chr2g0090431
rosa_laevigata RLG00000016108
rosa_multiflora Rmu_sc0004514.1_g000016
rosa_roxburghii Rroxscaffold_2G00151160
rosa_rugosa Rorug02G0008000
rosa_samantha Rh2AG053400 Rh2BG052700 Rh2CG054800 Rh2DG053600
rosa_wichuraiana Rw2G004360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1303
AccB1I GGYRCC 1 cut(s) 908
AciI CCGC 5 cut(s) 707, 760, 1271, 1277, 1283
AclWI GGATC 4 cut(s) 35, 169, 654, 789
AcoI YGGCCR 1 cut(s) 931
AcsI RAATTY 4 cut(s) 349, 518, 688, 1006
AdeI CACNNNGTG 1 cut(s) 1166
AfaI GTAC 2 cut(s) 1037, 1145
AfiI CCNNNNNNNGG 2 cut(s) 854, 940
AgsI TTSAA 9 cut(s) 13, 50, 182, 361, 373, 442, 524, 1063, 1306
AloI GAACNNNNNNTCC 2 cut(s) 18, 50
Alw21I GWGCWC 4 cut(s) 267, 699, 870, 1270
Alw26I GTCTC 4 cut(s) 152, 236, 446, 805
Alw44I GTGCAC 2 cut(s) 695, 1266
AlwI GGATC 4 cut(s) 35, 169, 654, 789
AlwNI CAGNNNCTG 1 cut(s) 140
AoxI GGCC 7 cut(s) 98, 412, 545, 856, 931, 1168, 1236
ApaLI GTGCAC 2 cut(s) 695, 1266
ApeKI GCWGC 3 cut(s) 140, 211, 313
ApoI RAATTY 4 cut(s) 349, 518, 688, 1006
ArsI GACNNNNNNTTYG 2 cut(s) 880, 912
AspLEI GCGC 2 cut(s) 84, 774
AspS9I GGNCC 4 cut(s) 98, 119, 545, 857
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 3 cut(s) 397, 503, 590
AvaII GGWCC 1 cut(s) 119
BaeGI GKGCMC 2 cut(s) 699, 1270
BalI TGGCCA 1 cut(s) 933
BanI GGYRCC 1 cut(s) 908
BanII GRGCYC 2 cut(s) 267, 870
Bbv12I GWGCWC 4 cut(s) 267, 699, 870, 1270
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 3 cut(s) 127, 223, 325
BccI CCATC 2 cut(s) 986, 1085
BceAI ACGGC 1 cut(s) 966
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 4 cut(s) 152, 236, 446, 805
BfaI CTAG 2 cut(s) 561, 677
BfmI CTRYAG 1 cut(s) 72
BfuAI ACCTGC 1 cut(s) 1303
BisI GCNGC 4 cut(s) 141, 212, 314, 707
BlpI GCTNAGC 1 cut(s) 130
BlsI GCNGC 4 cut(s) 142, 213, 315, 708
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 1 cut(s) 119
BmgT120I GGNCC 4 cut(s) 98, 119, 545, 857
BmiI GGNNCC 4 cut(s) 93, 99, 764, 910
BmrFI CCNGG 1 cut(s) 96
BmsI GCATC 5 cut(s) 705, 979, 1003, 1180, 1190
Bpu10I CCTNAGC 3 cut(s) 501, 849, 1233
Bpu1102I GCTNAGC 1 cut(s) 130
BpuEI CTTGAG 4 cut(s) 200, 740, 854, 884
BpuMI CCSGG 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 95
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 2 cut(s) 854, 940
Bse118I RCCGGY 2 cut(s) 100, 364
Bse1I ACTGG 3 cut(s) 493, 886, 934
Bse3DI GCAATG 1 cut(s) 1051
BseDI CCNNGG 1 cut(s) 95
BseGI GGATG 1 cut(s) 620
BseLI CCNNNNNNNGG 2 cut(s) 854, 940
BseMI GCAATG 1 cut(s) 1051
BseMII CTCAG 4 cut(s) 447, 492, 840, 1161
BseNI ACTGG 3 cut(s) 493, 886, 934
BseRI GAGGAG 1 cut(s) 464
BseSI GKGCMC 2 cut(s) 699, 1270
BseXI GCAGC 3 cut(s) 127, 223, 325
BseYI CCCAGC 1 cut(s) 1174
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 7 cut(s) 100, 414, 547, 858, 933, 1170, 1238
BshNI GGYRCC 1 cut(s) 908
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 4 cut(s) 267, 699, 870, 1270
BsiSI CCGG 5 cut(s) 95, 101, 126, 235, 365
BslI CCNNNNNNNGG 2 cut(s) 854, 940
BsmAI GTCTC 4 cut(s) 152, 236, 446, 805
BsmBI CGTCTC 1 cut(s) 236
BsmI GAATGC 2 cut(s) 162, 340
BsnI GGCC 7 cut(s) 100, 414, 547, 858, 933, 1170, 1238
Bsp1286I GDGCHC 4 cut(s) 267, 699, 870, 1270
Bsp143I GATC 5 cut(s) 40, 174, 646, 781, 921
Bsp1720I GCTNAGC 1 cut(s) 130
BspACI CCGC 5 cut(s) 707, 760, 1271, 1277, 1283
BspANI GGCC 7 cut(s) 100, 414, 547, 858, 933, 1170, 1238
BspCNI CTCAG 4 cut(s) 448, 493, 841, 1160
BspHI TCATGA 1 cut(s) 37
BspLI GGNNCC 4 cut(s) 93, 99, 764, 910
BspMI ACCTGC 1 cut(s) 1303
BspPI GGATC 4 cut(s) 35, 169, 654, 789
BspT107I GGYRCC 1 cut(s) 908
BsrDI GCAATG 1 cut(s) 1051
BsrFI RCCGGY 2 cut(s) 100, 364
BsrI ACTGG 3 cut(s) 493, 886, 934
BssAI RCCGGY 2 cut(s) 100, 364
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 5 cut(s) 40, 174, 646, 781, 921
Bst4CI ACNGT 3 cut(s) 230, 382, 845
Bst6I CTCTTC 1 cut(s) 991
BstAPI GCANNNNNTGC 3 cut(s) 140, 703, 715
BstC8I GCNNGC 3 cut(s) 366, 667, 1172
BstDEI CTNAG 7 cut(s) 130, 456, 501, 849, 918, 1147, 1233
BstEII GGTNACC 1 cut(s) 326
BstF5I GGATG 1 cut(s) 620
BstHHI GCGC 2 cut(s) 84, 774
BstKTI GATC 5 cut(s) 43, 177, 649, 784, 924
BstMAI GTCTC 4 cut(s) 152, 236, 446, 805
BstMBI GATC 5 cut(s) 40, 174, 646, 781, 921
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 7 cut(s) 59, 140, 168, 703, 715, 882, 1105
BstNSI RCATGY 1 cut(s) 1078
BstPI GGTNACC 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 94
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 2 cut(s) 699, 1270
BstV1I GCAGC 3 cut(s) 127, 223, 325
BstX2I RGATCY 1 cut(s) 646
BstYI RGATCY 1 cut(s) 646
BsuRI GGCC 7 cut(s) 100, 414, 547, 858, 933, 1170, 1238
BtsCI GGATG 1 cut(s) 620
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 3 cut(s) 486, 822, 1155
BveI ACCTGC 1 cut(s) 1303
Cac8I GCNNGC 3 cut(s) 366, 667, 1172
CaiI CAGNNNCTG 1 cut(s) 140
CciI TCATGA 1 cut(s) 37
CfoI GCGC 2 cut(s) 84, 774
Cfr10I RCCGGY 2 cut(s) 100, 364
Cfr13I GGNCC 4 cut(s) 98, 119, 545, 857
Csp6I GTAC 2 cut(s) 1036, 1144
CviAII CATG 9 cut(s) 38, 58, 274, 631, 745, 769, 811, 877, 1075
CviQI GTAC 2 cut(s) 1036, 1144
DdeI CTNAG 7 cut(s) 130, 456, 501, 849, 918, 1147, 1233
DpnI GATC 5 cut(s) 42, 176, 648, 783, 923
DpnII GATC 5 cut(s) 40, 174, 646, 781, 921
DraIII CACNNNGTG 1 cut(s) 1166
EaeI YGGCCR 1 cut(s) 931
Eam1104I CTCTTC 1 cut(s) 991
EarI CTCTTC 1 cut(s) 991
Ecl136II GAGCTC 2 cut(s) 265, 868
Eco24I GRGCYC 2 cut(s) 267, 870
Eco47I GGWCC 1 cut(s) 119
Eco53kI GAGCTC 2 cut(s) 265, 868
Eco91I GGTNACC 1 cut(s) 326
EcoICRI GAGCTC 2 cut(s) 265, 868
EcoO65I GGTNACC 1 cut(s) 326
EcoT38I GRGCYC 2 cut(s) 267, 870
Esp3I CGTCTC 1 cut(s) 236
FaeI CATG 9 cut(s) 41, 61, 277, 634, 748, 772, 814, 880, 1078
FalI AAGNNNNNCTT 2 cut(s) 702, 734
FatI CATG 9 cut(s) 37, 57, 273, 630, 744, 768, 810, 876, 1074
FauNDI CATATG 1 cut(s) 86
Fnu4HI GCNGC 4 cut(s) 141, 212, 314, 707
FokI GGATG 1 cut(s) 627
FriOI GRGCYC 2 cut(s) 267, 870
Fsp4HI GCNGC 4 cut(s) 141, 212, 314, 707
FspBI CTAG 2 cut(s) 561, 677
GlaI GCGC 2 cut(s) 83, 773
GluI GCNGC 4 cut(s) 141, 212, 314, 707
GsaI CCCAGC 1 cut(s) 1178
HaeIII GGCC 7 cut(s) 100, 414, 547, 858, 933, 1170, 1238
HapII CCGG 5 cut(s) 95, 101, 126, 235, 365
HhaI GCGC 2 cut(s) 84, 774
Hin1II CATG 9 cut(s) 41, 61, 277, 634, 748, 772, 814, 880, 1078
Hin6I GCGC 2 cut(s) 82, 772
HinP1I GCGC 2 cut(s) 82, 772
HindIII AAGCTT 1 cut(s) 169
HinfI GANTC 2 cut(s) 837, 889
HpaII CCGG 5 cut(s) 95, 101, 126, 235, 365
HphI GGTGA 3 cut(s) 397, 503, 590
Hpy166II GTNNAC 4 cut(s) 556, 697, 1158, 1268
Hpy188I TCNGA 3 cut(s) 113, 289, 1084
Hpy188III TCNNGA 7 cut(s) 38, 217, 848, 863, 871, 1114, 1187
Hpy8I GTNNAC 4 cut(s) 556, 697, 1158, 1268
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 6 cut(s) 17, 132, 518, 592, 681, 1057
HpyCH4III ACNGT 3 cut(s) 230, 382, 845
HpyCH4IV ACGT 1 cut(s) 1165
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 140, 168, 703, 715, 882, 1105
HpyF3I CTNAG 7 cut(s) 130, 456, 501, 849, 918, 1147, 1233
HpySE526I ACGT 1 cut(s) 1165
Hsp92II CATG 9 cut(s) 41, 61, 277, 634, 748, 772, 814, 880, 1078
HspAI GCGC 2 cut(s) 82, 772
KroI GCCGGC 1 cut(s) 364
KroNI GCCGGC 1 cut(s) 366
Kzo9I GATC 5 cut(s) 40, 174, 646, 781, 921
LmnI GCTCC 1 cut(s) 113
Lsp1109I GCAGC 3 cut(s) 127, 223, 325
LweI GCATC 5 cut(s) 705, 979, 1003, 1180, 1190
MaeI CTAG 2 cut(s) 561, 677
MaeII ACGT 1 cut(s) 1165
MaeIII GTNAC 2 cut(s) 326, 750
MalI GATC 5 cut(s) 42, 176, 648, 783, 923
MboI GATC 5 cut(s) 40, 174, 646, 781, 921
MboII GAAGA 1 cut(s) 1008
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 4 cut(s) 267, 699, 870, 1270
MlsI TGGCCA 1 cut(s) 933
MluCI AATT 9 cut(s) 17, 190, 254, 349, 518, 622, 688, 1006, 1306
MluNI TGGCCA 1 cut(s) 933
MlyI GAGTC 1 cut(s) 883
MmeI TCCRAC 2 cut(s) 91, 592
MnlI CCTC 7 cut(s) 442, 496, 628, 870, 1110, 1191, 1201
Mox20I TGGCCA 1 cut(s) 933
MroNI GCCGGC 1 cut(s) 364
MscI TGGCCA 1 cut(s) 933
MseI TTAA 2 cut(s) 426, 1309
MslI CAYNNNNRTG 1 cut(s) 272
Msp20I TGGCCA 1 cut(s) 933
MspI CCGG 5 cut(s) 95, 101, 126, 235, 365
MspR9I CCNGG 1 cut(s) 96
Mva1269I GAATGC 2 cut(s) 162, 340
MwoI GCNNNNNNNGC 7 cut(s) 59, 140, 168, 703, 715, 882, 1105
NaeI GCCGGC 1 cut(s) 366
NciI CCSGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 86
NdeII GATC 5 cut(s) 40, 174, 646, 781, 921
NgoMIV GCCGGC 1 cut(s) 364
NlaIII CATG 9 cut(s) 41, 61, 277, 634, 748, 772, 814, 880, 1078
NlaIV GGNNCC 4 cut(s) 93, 99, 764, 910
NmeAIII GCCGAG 1 cut(s) 795
NspI RCATGY 1 cut(s) 1078
PagI TCATGA 1 cut(s) 37
PctI GAATGC 2 cut(s) 162, 340
PdiI GCCGGC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 837
PkrI GCNGC 4 cut(s) 142, 213, 315, 708
PleI GAGTC 1 cut(s) 883
PpsI GAGTC 1 cut(s) 883
Psp124BI GAGCTC 2 cut(s) 267, 870
PspEI GGTNACC 1 cut(s) 326
PspFI CCCAGC 1 cut(s) 1174
PspN4I GGNNCC 4 cut(s) 93, 99, 764, 910
PspPI GGNCC 4 cut(s) 98, 119, 545, 857
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 1 cut(s) 646
RsaI GTAC 2 cut(s) 1037, 1145
RsaNI GTAC 2 cut(s) 1036, 1144
RseI CAYNNNNRTG 1 cut(s) 272
SacI GAGCTC 2 cut(s) 267, 870
SaqAI TTAA 2 cut(s) 426, 1309
SatI GCNGC 4 cut(s) 141, 212, 314, 707
Sau3AI GATC 5 cut(s) 40, 174, 646, 781, 921
Sau96I GGNCC 4 cut(s) 98, 119, 545, 857
SchI GAGTC 1 cut(s) 883
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 4 cut(s) 267, 699, 870, 1270
SfaNI GCATC 5 cut(s) 705, 979, 1003, 1180, 1190
SfcI CTRYAG 1 cut(s) 72
SinI GGWCC 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 272
SmlI CTYRAG 4 cut(s) 215, 755, 863, 869
SmoI CTYRAG 4 cut(s) 215, 755, 863, 869
Sse9I AATT 9 cut(s) 17, 190, 254, 349, 518, 622, 688, 1006, 1306
SsiI CCGC 5 cut(s) 707, 760, 1271, 1277, 1283
SspMI CTAG 2 cut(s) 561, 677
SstI GAGCTC 2 cut(s) 267, 870
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 3 cut(s) 230, 382, 845
TaiI ACGT 1 cut(s) 1168
TaqI TCGA 2 cut(s) 1011, 1102
TasI AATT 9 cut(s) 17, 190, 254, 349, 518, 622, 688, 1006, 1306
TatI WGTACW 1 cut(s) 1143
TauI GCSGC 1 cut(s) 709
TfiI GAWTC 1 cut(s) 837
Tru1I TTAA 2 cut(s) 426, 1309
Tru9I TTAA 2 cut(s) 426, 1309
TscAI CASTG 3 cut(s) 493, 829, 1155
TseI GCWGC 3 cut(s) 140, 211, 313
TspDTI ATGAA 3 cut(s) 26, 230, 799
TspRI CASTG 3 cut(s) 493, 829, 1155
VneI GTGCAC 2 cut(s) 695, 1266
VpaK11BI GGWCC 1 cut(s) 119
XapI RAATTY 4 cut(s) 349, 518, 688, 1006
XceI RCATGY 1 cut(s) 1078
XcmI CCANNNNNNNNNTGG 2 cut(s) 775, 1246
XspI CTAG 2 cut(s) 561, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.