Disease resistance protein (TIR-NBS-LRR class)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 47, 603, 1961 |
| AatII | GACGTC | 2 cut(s) | 627, 3024 |
| AbsI | CCTCGAGG | 1 cut(s) | 1838 |
| AccI | GTMKAC | 3 cut(s) | 1600, 2721, 3092 |
| AccII | CGCG | 2 cut(s) | 588, 2953 |
| AccIII | TCCGGA | 2 cut(s) | 3100, 3136 |
| AciI | CCGC | 4 cut(s) | 2595, 3069, 3226, 3232 |
| AclI | AACGTT | 1 cut(s) | 1262 |
| AcoI | YGGCCR | 2 cut(s) | 51, 3277 |
| AcuI | CTGAAG | 2 cut(s) | 1310, 2363 |
| AcvI | CACGTG | 1 cut(s) | 111 |
| AcyI | GRCGYC | 3 cut(s) | 624, 2148, 3021 |
| AdeI | CACNNNGTG | 1 cut(s) | 114 |
| AfaI | GTAC | 4 cut(s) | 538, 2448, 2646, 3170 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 905, 2224 |
| AflII | CTTAAG | 2 cut(s) | 917, 1907 |
| AflIII | ACRYGT | 1 cut(s) | 2313 |
| AhlI | ACTAGT | 1 cut(s) | 2564 |
| AjiI | CACGTC | 1 cut(s) | 1470 |
| AjnI | CCWGG | 3 cut(s) | 1372, 2032, 2998 |
| Alw21I | GWGCWC | 3 cut(s) | 553, 1089, 2405 |
| Alw26I | GTCTC | 6 cut(s) | 631, 1025, 1772, 2812, 3147, 3200 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3296 |
| Ama87I | CYCGRG | 2 cut(s) | 1838, 2500 |
| Aor13HI | TCCGGA | 2 cut(s) | 3100, 3136 |
| AoxI | GGCC | 5 cut(s) | 51, 344, 1609, 2226, 3277 |
| ApeKI | GCWGC | 3 cut(s) | 24, 356, 2948 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 1878, 2478, 2607, 2693, 2930 |
| AspA2I | CCTAGG | 1 cut(s) | 2965 |
| AspLEI | GCGC | 2 cut(s) | 590, 2953 |
| AspS9I | GGNCC | 2 cut(s) | 344, 783 |
| AsuC2I | CCSGG | 2 cut(s) | 325, 1936 |
| AsuHPI | GGTGA | 5 cut(s) | 187, 434, 901, 2284, 2779 |
| AsuII | TTCGAA | 1 cut(s) | 389 |
| AvaI | CYCGRG | 2 cut(s) | 1838, 2500 |
| AvaII | GGWCC | 1 cut(s) | 783 |
| AvrII | CCTAGG | 1 cut(s) | 2965 |
| AxyI | CCTNAGG | 1 cut(s) | 2057 |
| BaeGI | GKGCMC | 1 cut(s) | 1729 |
| BalI | TGGCCA | 1 cut(s) | 3279 |
| BanII | GRGCYC | 2 cut(s) | 553, 1089 |
| BbrPI | CACGTG | 1 cut(s) | 111 |
| BbsI | GAAGAC | 1 cut(s) | 2996 |
| Bbv12I | GWGCWC | 3 cut(s) | 553, 1089, 2405 |
| BbvCI | CCTCAGC | 1 cut(s) | 1401 |
| BbvI | GCAGC | 3 cut(s) | 11, 368, 2935 |
| BccI | CCATC | 8 cut(s) | 282, 591, 907, 1063, 1220, 1226, 2397, 3143 |
| BceAI | ACGGC | 4 cut(s) | 25, 66, 208, 1624 |
| BciT130I | CCWGG | 3 cut(s) | 1374, 2034, 3000 |
| BclI | TGATCA | 2 cut(s) | 834, 1561 |
| BcnI | CCSGG | 2 cut(s) | 325, 1936 |
| BcoDI | GTCTC | 6 cut(s) | 631, 1025, 1772, 2812, 3147, 3200 |
| BcuI | ACTAGT | 1 cut(s) | 2564 |
| BfmI | CTRYAG | 3 cut(s) | 540, 1305, 1338 |
| BfrI | CTTAAG | 2 cut(s) | 917, 1907 |
| BglII | AGATCT | 2 cut(s) | 1816, 1849 |
| BisI | GCNGC | 4 cut(s) | 25, 357, 2949, 3070 |
| BlnI | CCTAGG | 1 cut(s) | 2965 |
| BlpI | GCTNAGC | 1 cut(s) | 1083 |
| BlsI | GCNGC | 4 cut(s) | 26, 358, 2950, 3071 |
| BmcAI | AGTACT | 2 cut(s) | 538, 2448 |
| Bme1390I | CCNGG | 5 cut(s) | 325, 1374, 1936, 2034, 3000 |
| Bme18I | GGWCC | 1 cut(s) | 783 |
| BmeT110I | CYCGRG | 2 cut(s) | 1838, 2500 |
| BmgBI | CACGTC | 1 cut(s) | 1470 |
| BmgT120I | GGNCC | 2 cut(s) | 344, 783 |
| BmrFI | CCNGG | 5 cut(s) | 325, 1374, 1936, 2034, 3000 |
| BmsI | GCATC | 7 cut(s) | 21, 1065, 1302, 2511, 3056, 3219, 3385 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2990 |
| BpiI | GAAGAC | 1 cut(s) | 2996 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 2483, 2515, 3104, 3136 |
| BpmI | CTGGAG | 4 cut(s) | 1412, 2258, 3204, 3368 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1401 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1083 |
| Bpu14I | TTCGAA | 1 cut(s) | 389 |
| BpuEI | CTTGAG | 9 cut(s) | 825, 842, 888, 1751, 1754, 2150, 2153, 2389, 2624 |
| BpuMI | CCSGG | 2 cut(s) | 325, 1936 |
| Bsa29I | ATCGAT | 2 cut(s) | 1488, 2519 |
| BsaAI | YACGTR | 2 cut(s) | 111, 1628 |
| BsaHI | GRCGYC | 3 cut(s) | 624, 2148, 3021 |
| BsaI | GGTCTC | 2 cut(s) | 1025, 2812 |
| BsaWI | WCCGGW | 4 cut(s) | 181, 2764, 3100, 3136 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 905, 2224 |
| Bse118I | RCCGGY | 2 cut(s) | 1001, 1433 |
| Bse1I | ACTGG | 4 cut(s) | 574, 612, 1574, 2152 |
| Bse21I | CCTNAGG | 1 cut(s) | 2057 |
| Bse3DI | GCAATG | 1 cut(s) | 1176 |
| BseAI | TCCGGA | 2 cut(s) | 3100, 3136 |
| BseBI | CCWGG | 3 cut(s) | 1374, 2034, 3000 |
| BseCI | ATCGAT | 2 cut(s) | 1488, 2519 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 905, 2224 |
| BseMI | GCAATG | 1 cut(s) | 1176 |
| BseNI | ACTGG | 4 cut(s) | 574, 612, 1574, 2152 |
| BseSI | GKGCMC | 1 cut(s) | 1729 |
| BseXI | GCAGC | 3 cut(s) | 11, 368, 2935 |
| Bsh1236I | CGCG | 2 cut(s) | 588, 2953 |
| BshFI | GGCC | 5 cut(s) | 53, 346, 1611, 2228, 3279 |
| BshVI | ATCGAT | 2 cut(s) | 1488, 2519 |
| BsiHKAI | GWGCWC | 3 cut(s) | 553, 1089, 2405 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1838, 2500 |
| BsiSI | CCGG | 8 cut(s) | 182, 324, 1002, 1434, 1936, 2765, 3101, 3137 |
| BslFI | GGGAC | 3 cut(s) | 528, 1261, 3064 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 905, 2224 |
| BsmAI | GTCTC | 6 cut(s) | 631, 1025, 1772, 2812, 3147, 3200 |
| BsmBI | CGTCTC | 1 cut(s) | 631 |
| BsmFI | GGGAC | 3 cut(s) | 528, 1261, 3064 |
| BsmI | GAATGC | 2 cut(s) | 1402, 2336 |
| BsnI | GGCC | 5 cut(s) | 53, 346, 1611, 2228, 3279 |
| Bso31I | GGTCTC | 2 cut(s) | 1025, 2812 |
| BsoBI | CYCGRG | 2 cut(s) | 1838, 2500 |
| Bsp119I | TTCGAA | 1 cut(s) | 389 |
| Bsp1286I | GDGCHC | 4 cut(s) | 553, 1089, 1729, 2405 |
| Bsp13I | TCCGGA | 2 cut(s) | 3100, 3136 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1083 |
| Bsp19I | CCATGG | 2 cut(s) | 360, 3341 |
| BspACI | CCGC | 4 cut(s) | 2595, 3069, 3226, 3232 |
| BspANI | GGCC | 5 cut(s) | 53, 346, 1611, 2228, 3279 |
| BspDI | ATCGAT | 2 cut(s) | 1488, 2519 |
| BspEI | TCCGGA | 2 cut(s) | 3100, 3136 |
| BspFNI | CGCG | 2 cut(s) | 588, 2953 |
| BspHI | TCATGA | 2 cut(s) | 865, 1558 |
| BspQI | GCTCTTC | 1 cut(s) | 541 |
| BspT104I | TTCGAA | 1 cut(s) | 389 |
| BspTI | CTTAAG | 2 cut(s) | 917, 1907 |
| BspTNI | GGTCTC | 2 cut(s) | 1025, 2812 |
| BsrDI | GCAATG | 1 cut(s) | 1176 |
| BsrFI | RCCGGY | 2 cut(s) | 1001, 1433 |
| BsrI | ACTGG | 4 cut(s) | 574, 612, 1574, 2152 |
| BssAI | RCCGGY | 2 cut(s) | 1001, 1433 |
| BssNI | GRCGYC | 3 cut(s) | 624, 2148, 3021 |
| BssT1I | CCWWGG | 7 cut(s) | 360, 483, 786, 1030, 1525, 2965, 3341 |
| Bst2UI | CCWGG | 3 cut(s) | 1374, 2034, 3000 |
| Bst4CI | ACNGT | 8 cut(s) | 471, 519, 1647, 2110, 2386, 2735, 3180, 3298 |
| Bst6I | CTCTTC | 3 cut(s) | 541, 1056, 1350 |
| BstACI | GRCGYC | 3 cut(s) | 624, 2148, 3021 |
| BstAFI | CTTAAG | 2 cut(s) | 917, 1907 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 998, 1321 |
| BstBAI | YACGTR | 2 cut(s) | 111, 1628 |
| BstBI | TTCGAA | 1 cut(s) | 389 |
| BstC8I | GCNNGC | 3 cut(s) | 999, 1080, 1532 |
| BstDSI | CCRYGG | 2 cut(s) | 360, 3341 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2222 |
| BstFNI | CGCG | 2 cut(s) | 588, 2953 |
| BstHHI | GCGC | 2 cut(s) | 590, 2953 |
| BstMAI | GTCTC | 6 cut(s) | 631, 1025, 1772, 2812, 3147, 3200 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 998, 1084, 1321, 2134, 2594 |
| BstNI | CCWGG | 3 cut(s) | 1374, 2034, 3000 |
| BstNSI | RCATGY | 3 cut(s) | 258, 1534, 2128 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2990 |
| BstSCI | CCNGG | 5 cut(s) | 323, 1372, 1934, 2032, 2998 |
| BstSFI | CTRYAG | 3 cut(s) | 540, 1305, 1338 |
| BstSLI | GKGCMC | 1 cut(s) | 1729 |
| BstUI | CGCG | 2 cut(s) | 588, 2953 |
| BstV1I | GCAGC | 3 cut(s) | 11, 368, 2935 |
| BstV2I | GAAGAC | 1 cut(s) | 2996 |
| BstX2I | RGATCY | 9 cut(s) | 69, 135, 331, 812, 1147, 1816, 1849, 2179, 2248 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 2159, 2236, 3182 |
| BstYI | RGATCY | 9 cut(s) | 69, 135, 331, 812, 1147, 1816, 1849, 2179, 2248 |
| Bsu15I | ATCGAT | 2 cut(s) | 1488, 2519 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2057 |
| BsuRI | GGCC | 5 cut(s) | 53, 346, 1611, 2228, 3279 |
| BsuTUI | ATCGAT | 2 cut(s) | 1488, 2519 |
| BtgI | CCRYGG | 2 cut(s) | 360, 3341 |
| BtgZI | GCGATG | 2 cut(s) | 252, 569 |
| BtrI | CACGTC | 1 cut(s) | 1470 |
| BtsI | GCAGTG | 1 cut(s) | 2463 |
| BtsIMutI | CAGTG | 6 cut(s) | 619, 1030, 1437, 2115, 2463, 3294 |
| Cac8I | GCNNGC | 3 cut(s) | 999, 1080, 1532 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3296 |
| CciI | TCATGA | 2 cut(s) | 865, 1558 |
| CfoI | GCGC | 2 cut(s) | 590, 2953 |
| Cfr10I | RCCGGY | 2 cut(s) | 1001, 1433 |
| Cfr13I | GGNCC | 2 cut(s) | 344, 783 |
| ClaI | ATCGAT | 2 cut(s) | 1488, 2519 |
| CseI | GACGC | 2 cut(s) | 1586, 2137 |
| CsiI | ACCWGGT | 1 cut(s) | 2998 |
| Csp6I | GTAC | 4 cut(s) | 537, 2447, 2645, 3169 |
| CviQI | GTAC | 4 cut(s) | 537, 2447, 2645, 3169 |
| DraI | TTTAAA | 3 cut(s) | 247, 1044, 2917 |
| DraIII | CACNNNGTG | 1 cut(s) | 114 |
| EaeI | YGGCCR | 2 cut(s) | 51, 3277 |
| Eam1104I | CTCTTC | 3 cut(s) | 541, 1056, 1350 |
| EarI | CTCTTC | 3 cut(s) | 541, 1056, 1350 |
| Ecl136II | GAGCTC | 2 cut(s) | 551, 1087 |
| Eco130I | CCWWGG | 7 cut(s) | 360, 483, 786, 1030, 1525, 2965, 3341 |
| Eco24I | GRGCYC | 2 cut(s) | 553, 1089 |
| Eco31I | GGTCTC | 2 cut(s) | 1025, 2812 |
| Eco32I | GATATC | 1 cut(s) | 2362 |
| Eco47I | GGWCC | 1 cut(s) | 783 |
| Eco53kI | GAGCTC | 2 cut(s) | 551, 1087 |
| Eco57I | CTGAAG | 2 cut(s) | 1310, 2363 |
| Eco72I | CACGTG | 1 cut(s) | 111 |
| Eco81I | CCTNAGG | 1 cut(s) | 2057 |
| Eco88I | CYCGRG | 2 cut(s) | 1838, 2500 |
| EcoICRI | GAGCTC | 2 cut(s) | 551, 1087 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2222 |
| EcoO109I | RGGNCCY | 1 cut(s) | 783 |
| EcoRI | GAATTC | 1 cut(s) | 1789 |
| EcoRII | CCWGG | 3 cut(s) | 1372, 2032, 2998 |
| EcoRV | GATATC | 1 cut(s) | 2362 |
| EcoT14I | CCWWGG | 7 cut(s) | 360, 483, 786, 1030, 1525, 2965, 3341 |
| EcoT38I | GRGCYC | 2 cut(s) | 553, 1089 |
| ErhI | CCWWGG | 7 cut(s) | 360, 483, 786, 1030, 1525, 2965, 3341 |
| Esp3I | CGTCTC | 1 cut(s) | 631 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1518, 1550, 2708, 2740 |
| FaqI | GGGAC | 3 cut(s) | 528, 1261, 3064 |
| FbaI | TGATCA | 2 cut(s) | 834, 1561 |
| FblI | GTMKAC | 3 cut(s) | 1600, 2721, 3092 |
| Fnu4HI | GCNGC | 4 cut(s) | 25, 357, 2949, 3070 |
| FriOI | GRGCYC | 2 cut(s) | 553, 1089 |
| Fsp4HI | GCNGC | 4 cut(s) | 25, 357, 2949, 3070 |
| GlaI | GCGC | 2 cut(s) | 589, 2952 |
| GluI | GCNGC | 4 cut(s) | 25, 357, 2949, 3070 |
| GsuI | CTGGAG | 4 cut(s) | 1412, 2258, 3204, 3368 |
| HaeIII | GGCC | 5 cut(s) | 53, 346, 1611, 2228, 3279 |
| HapII | CCGG | 8 cut(s) | 182, 324, 1002, 1434, 1936, 2765, 3101, 3137 |
| HgaI | GACGC | 2 cut(s) | 1586, 2137 |
| HhaI | GCGC | 2 cut(s) | 590, 2953 |
| Hin1I | GRCGYC | 3 cut(s) | 624, 2148, 3021 |
| Hin6I | GCGC | 2 cut(s) | 588, 2951 |
| HinP1I | GCGC | 2 cut(s) | 588, 2951 |
| HincII | GTYRAC | 3 cut(s) | 563, 1246, 2722 |
| HindII | GTYRAC | 3 cut(s) | 563, 1246, 2722 |
| HindIII | AAGCTT | 2 cut(s) | 995, 3357 |
| HpaI | GTTAAC | 1 cut(s) | 1246 |
| HpaII | CCGG | 8 cut(s) | 182, 324, 1002, 1434, 1936, 2765, 3101, 3137 |
| HphI | GGTGA | 5 cut(s) | 187, 434, 901, 2284, 2779 |
| Hpy166II | GTNNAC | 7 cut(s) | 563, 1246, 1601, 1618, 1631, 2722, 3093 |
| Hpy8I | GTNNAC | 7 cut(s) | 563, 1246, 1601, 1618, 1631, 2722, 3093 |
| Hpy99I | CGWCG | 1 cut(s) | 1386 |
| HpyAV | CCTTC | 8 cut(s) | 213, 758, 1044, 1686, 2138, 2292, 2338, 2710 |
| HpyCH4III | ACNGT | 8 cut(s) | 471, 519, 1647, 2110, 2386, 2735, 3180, 3298 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 998, 1084, 1321, 2134, 2594 |
| Hsp92I | GRCGYC | 3 cut(s) | 624, 2148, 3021 |
| HspAI | GCGC | 2 cut(s) | 588, 2951 |
| Kpn2I | TCCGGA | 2 cut(s) | 3100, 3136 |
| Ksp22I | TGATCA | 2 cut(s) | 834, 1561 |
| KspAI | GTTAAC | 1 cut(s) | 1246 |
| LguI | GCTCTTC | 1 cut(s) | 541 |
| LmnI | GCTCC | 4 cut(s) | 715, 900, 1053, 1092 |
| Lsp1109I | GCAGC | 3 cut(s) | 11, 368, 2935 |
| LweI | GCATC | 7 cut(s) | 21, 1065, 1302, 2511, 3056, 3219, 3385 |
| MabI | ACCWGGT | 1 cut(s) | 2998 |
| MaeIII | GTNAC | 9 cut(s) | 146, 422, 620, 1856, 2277, 2767, 2828, 3160, 3286 |
| MfeI | CAATTG | 1 cut(s) | 2289 |
| MflI | RGATCY | 9 cut(s) | 69, 135, 331, 812, 1147, 1816, 1849, 2179, 2248 |
| MhlI | GDGCHC | 4 cut(s) | 553, 1089, 1729, 2405 |
| MlsI | TGGCCA | 1 cut(s) | 3279 |
| MluNI | TGGCCA | 1 cut(s) | 3279 |
| MlyI | GAGTC | 5 cut(s) | 1137, 1367, 2031, 3072, 3149 |
| MmeI | TCCRAC | 4 cut(s) | 412, 1178, 1245, 1818 |
| Mox20I | TGGCCA | 1 cut(s) | 3279 |
| MroI | TCCGGA | 2 cut(s) | 3100, 3136 |
| MroXI | GAANNNNTTC | 5 cut(s) | 1878, 2478, 2607, 2693, 2930 |
| MscI | TGGCCA | 1 cut(s) | 3279 |
| MslI | CAYNNNNRTG | 1 cut(s) | 705 |
| Msp20I | TGGCCA | 1 cut(s) | 3279 |
| MspCI | CTTAAG | 2 cut(s) | 917, 1907 |
| MspI | CCGG | 8 cut(s) | 182, 324, 1002, 1434, 1936, 2765, 3101, 3137 |
| MspR9I | CCNGG | 5 cut(s) | 325, 1374, 1936, 2034, 3000 |
| MunI | CAATTG | 1 cut(s) | 2289 |
| Mva1269I | GAATGC | 2 cut(s) | 1402, 2336 |
| MvaI | CCWGG | 3 cut(s) | 1374, 2034, 3000 |
| MvnI | CGCG | 2 cut(s) | 588, 2953 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 998, 1084, 1321, 2134, 2594 |
| NciI | CCSGG | 2 cut(s) | 325, 1936 |
| NcoI | CCATGG | 2 cut(s) | 360, 3341 |
| NmuCI | GTSAC | 5 cut(s) | 146, 422, 620, 2767, 2828 |
| NspI | RCATGY | 3 cut(s) | 258, 1534, 2128 |
| NspV | TTCGAA | 1 cut(s) | 389 |
| PaeI | GCATGC | 1 cut(s) | 1534 |
| PaeR7I | CTCGAG | 2 cut(s) | 1838, 2500 |
| PagI | TCATGA | 2 cut(s) | 865, 1558 |
| PciSI | GCTCTTC | 1 cut(s) | 541 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1422, 2046 |
| PctI | GAATGC | 2 cut(s) | 1402, 2336 |
| PdmI | GAANNNNTTC | 5 cut(s) | 1878, 2478, 2607, 2693, 2930 |
| PfeI | GAWTC | 9 cut(s) | 178, 479, 1478, 1901, 2026, 2296, 3131, 3317, 3371 |
| PflFI | GACNNNGTC | 1 cut(s) | 428 |
| PfoI | TCCNGGA | 1 cut(s) | 1372 |
| PkrI | GCNGC | 4 cut(s) | 26, 358, 2950, 3071 |
| PleI | GAGTC | 5 cut(s) | 1136, 1366, 2031, 3072, 3149 |
| PmaCI | CACGTG | 1 cut(s) | 111 |
| PmlI | CACGTG | 1 cut(s) | 111 |
| PpsI | GAGTC | 5 cut(s) | 1136, 1366, 2031, 3072, 3149 |
| Ppu21I | YACGTR | 2 cut(s) | 111, 1628 |
| PpuMI | RGGWCCY | 1 cut(s) | 783 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2990 |
| PsiI | TTATAA | 3 cut(s) | 47, 603, 1961 |
| Psp124BI | GAGCTC | 2 cut(s) | 553, 1089 |
| Psp1406I | AACGTT | 1 cut(s) | 1262 |
| Psp5II | RGGWCCY | 1 cut(s) | 783 |
| Psp6I | CCWGG | 3 cut(s) | 1372, 2032, 2998 |
| PspCI | CACGTG | 1 cut(s) | 111 |
| PspGI | CCWGG | 3 cut(s) | 1372, 2032, 2998 |
| PspPI | GGNCC | 2 cut(s) | 344, 783 |
| PspPPI | RGGWCCY | 1 cut(s) | 783 |
| PspXI | VCTCGAGB | 1 cut(s) | 1838 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3296 |
| PsuI | RGATCY | 9 cut(s) | 69, 135, 331, 812, 1147, 1816, 1849, 2179, 2248 |
| PsyI | GACNNNGTC | 1 cut(s) | 428 |
| RsaI | GTAC | 4 cut(s) | 538, 2448, 2646, 3170 |
| RsaNI | GTAC | 4 cut(s) | 537, 2447, 2645, 3169 |
| RseI | CAYNNNNRTG | 1 cut(s) | 705 |
| SacI | GAGCTC | 2 cut(s) | 553, 1089 |
| SalI | GTCGAC | 1 cut(s) | 2720 |
| SapI | GCTCTTC | 1 cut(s) | 541 |
| SatI | GCNGC | 4 cut(s) | 25, 357, 2949, 3070 |
| Sau96I | GGNCC | 2 cut(s) | 344, 783 |
| ScaI | AGTACT | 2 cut(s) | 538, 2448 |
| SchI | GAGTC | 5 cut(s) | 1137, 1367, 2031, 3072, 3149 |
| ScrFI | CCNGG | 5 cut(s) | 325, 1374, 1936, 2034, 3000 |
| SduI | GDGCHC | 4 cut(s) | 553, 1089, 1729, 2405 |
| SexAI | ACCWGGT | 1 cut(s) | 2998 |
| SfaNI | GCATC | 7 cut(s) | 21, 1065, 1302, 2511, 3056, 3219, 3385 |
| SfcI | CTRYAG | 3 cut(s) | 540, 1305, 1338 |
| Sfr274I | CTCGAG | 2 cut(s) | 1838, 2500 |
| SfuI | TTCGAA | 1 cut(s) | 389 |
| SinI | GGWCC | 1 cut(s) | 783 |
| SlaI | CTCGAG | 2 cut(s) | 1838, 2500 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 705 |
| SpeI | ACTAGT | 1 cut(s) | 2564 |
| SphI | GCATGC | 1 cut(s) | 1534 |
| SsiI | CCGC | 4 cut(s) | 2595, 3069, 3226, 3232 |
| SspI | AATATT | 2 cut(s) | 1555, 3260 |
| SstI | GAGCTC | 2 cut(s) | 553, 1089 |
| StyD4I | CCNGG | 5 cut(s) | 323, 1372, 1934, 2032, 2998 |
| StyI | CCWWGG | 7 cut(s) | 360, 483, 786, 1030, 1525, 2965, 3341 |
| TaaI | ACNGT | 8 cut(s) | 471, 519, 1647, 2110, 2386, 2735, 3180, 3298 |
| TatI | WGTACW | 2 cut(s) | 536, 2446 |
| TauI | GCSGC | 1 cut(s) | 3072 |
| TfiI | GAWTC | 9 cut(s) | 178, 479, 1478, 1901, 2026, 2296, 3131, 3317, 3371 |
| TscAI | CASTG | 6 cut(s) | 619, 1030, 1444, 2115, 2470, 3301 |
| TseFI | GTSAC | 5 cut(s) | 146, 422, 620, 2767, 2828 |
| TseI | GCWGC | 3 cut(s) | 24, 356, 2948 |
| Tsp45I | GTSAC | 5 cut(s) | 146, 422, 620, 2767, 2828 |
| TspGWI | ACGGA | 1 cut(s) | 3291 |
| TspRI | CASTG | 6 cut(s) | 619, 1030, 1444, 2115, 2470, 3301 |
| Tth111I | GACNNNGTC | 1 cut(s) | 428 |
| Vha464I | CTTAAG | 2 cut(s) | 917, 1907 |
| VpaK11BI | GGWCC | 1 cut(s) | 783 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2222 |
| XbaI | TCTAGA | 3 cut(s) | 769, 1411, 2455 |
| XceI | RCATGY | 3 cut(s) | 258, 1534, 2128 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 938, 3179 |
| XhoI | CTCGAG | 2 cut(s) | 1838, 2500 |
| XmaJI | CCTAGG | 1 cut(s) | 2965 |
| XmiI | GTMKAC | 3 cut(s) | 1600, 2721, 3092 |
| XmnI | GAANNNNTTC | 5 cut(s) | 1878, 2478, 2607, 2693, 2930 |
| ZraI | GACGTC | 2 cut(s) | 625, 3022 |
| ZrmI | AGTACT | 2 cut(s) | 538, 2448 |