Disease resistance protein (TIR-NBS-LRR class)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 179, 735 |
| AatII | GACGTC | 1 cut(s) | 759 |
| AccI | GTMKAC | 2 cut(s) | 1732, 2847 |
| AccII | CGCG | 2 cut(s) | 720, 1601 |
| AciI | CCGC | 2 cut(s) | 112, 2721 |
| AclI | AACGTT | 2 cut(s) | 80, 1394 |
| AcoI | YGGCCR | 1 cut(s) | 183 |
| AcuI | CTGAAG | 3 cut(s) | 40, 1442, 2489 |
| AcvI | CACGTG | 1 cut(s) | 243 |
| AcyI | GRCGYC | 2 cut(s) | 756, 2274 |
| AdeI | CACNNNGTG | 1 cut(s) | 246 |
| AfaI | GTAC | 4 cut(s) | 670, 2045, 2574, 2944 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1037 |
| AflII | CTTAAG | 2 cut(s) | 1049, 2837 |
| AflIII | ACRYGT | 1 cut(s) | 2439 |
| AhlI | ACTAGT | 1 cut(s) | 2690 |
| AjnI | CCWGG | 2 cut(s) | 1504, 2158 |
| AleI | CACNNNNGTG | 1 cut(s) | 2897 |
| Alw21I | GWGCWC | 3 cut(s) | 685, 1221, 2531 |
| Alw26I | GTCTC | 5 cut(s) | 763, 1157, 2081, 2159, 2943 |
| Ama87I | CYCGRG | 3 cut(s) | 1970, 2039, 2626 |
| AoxI | GGCC | 4 cut(s) | 183, 476, 1741, 2352 |
| ApeKI | GCWGC | 2 cut(s) | 156, 488 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 2010, 2604, 2733, 2819 |
| AspLEI | GCGC | 3 cut(s) | 106, 722, 1601 |
| AspS9I | GGNCC | 2 cut(s) | 476, 915 |
| AsuC2I | CCSGG | 2 cut(s) | 457, 1567 |
| AsuHPI | GGTGA | 7 cut(s) | 319, 566, 1033, 1861, 2377, 2410, 2417 |
| AsuII | TTCGAA | 2 cut(s) | 521, 2759 |
| AvaI | CYCGRG | 3 cut(s) | 1970, 2039, 2626 |
| AvaII | GGWCC | 1 cut(s) | 915 |
| AxyI | CCTNAGG | 1 cut(s) | 2183 |
| BanII | GRGCYC | 2 cut(s) | 685, 1221 |
| BauI | CACGAG | 1 cut(s) | 2946 |
| BbrPI | CACGTG | 1 cut(s) | 243 |
| Bbv12I | GWGCWC | 3 cut(s) | 685, 1221, 2531 |
| BbvCI | CCTCAGC | 2 cut(s) | 86, 1533 |
| BbvI | GCAGC | 2 cut(s) | 143, 500 |
| BccI | CCATC | 8 cut(s) | 99, 414, 723, 1039, 1195, 1352, 1358, 2296 |
| BceAI | ACGGC | 4 cut(s) | 157, 198, 340, 1756 |
| BciT130I | CCWGG | 2 cut(s) | 1506, 2160 |
| BclI | TGATCA | 3 cut(s) | 966, 1693, 2914 |
| BcnI | CCSGG | 2 cut(s) | 457, 1567 |
| BcoDI | GTCTC | 5 cut(s) | 763, 1157, 2081, 2159, 2943 |
| BcuI | ACTAGT | 1 cut(s) | 2690 |
| BfmI | CTRYAG | 3 cut(s) | 672, 1437, 1470 |
| BfoI | RGCGCY | 1 cut(s) | 107 |
| BfrI | CTTAAG | 2 cut(s) | 1049, 2837 |
| BglII | AGATCT | 2 cut(s) | 1948, 2341 |
| BisI | GCNGC | 2 cut(s) | 157, 489 |
| BlpI | GCTNAGC | 1 cut(s) | 1215 |
| BlsI | GCNGC | 2 cut(s) | 158, 490 |
| BmcAI | AGTACT | 1 cut(s) | 670 |
| Bme1390I | CCNGG | 4 cut(s) | 457, 1506, 1567, 2160 |
| Bme18I | GGWCC | 1 cut(s) | 915 |
| BmeT110I | CYCGRG | 3 cut(s) | 1970, 2039, 2626 |
| BmgT120I | GGNCC | 2 cut(s) | 476, 915 |
| BmrFI | CCNGG | 4 cut(s) | 457, 1506, 1567, 2160 |
| BmsI | GCATC | 6 cut(s) | 153, 1197, 1434, 2051, 2637, 2928 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2609, 2641 |
| BpmI | CTGGAG | 1 cut(s) | 1544 |
| Bpu10I | CCTNAGC | 2 cut(s) | 86, 1533 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1215 |
| Bpu14I | TTCGAA | 2 cut(s) | 521, 2759 |
| BpuEI | CTTGAG | 8 cut(s) | 957, 974, 1020, 1883, 2276, 2315, 2515, 2750 |
| BpuMI | CCSGG | 2 cut(s) | 457, 1567 |
| Bsa29I | ATCGAT | 1 cut(s) | 2645 |
| BsaAI | YACGTR | 2 cut(s) | 243, 1760 |
| BsaHI | GRCGYC | 2 cut(s) | 756, 2274 |
| BsaI | GGTCTC | 1 cut(s) | 1157 |
| BsaJI | CCNNGG | 9 cut(s) | 456, 492, 615, 918, 1162, 1357, 1657, 2158, 2284 |
| BsaWI | WCCGGW | 1 cut(s) | 313 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 839, 869, 1353, 1383 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1037 |
| Bse118I | RCCGGY | 1 cut(s) | 1133 |
| Bse1I | ACTGG | 3 cut(s) | 706, 744, 2278 |
| Bse21I | CCTNAGG | 1 cut(s) | 2183 |
| Bse3DI | GCAATG | 1 cut(s) | 1308 |
| BseBI | CCWGG | 2 cut(s) | 1506, 2160 |
| BseCI | ATCGAT | 1 cut(s) | 2645 |
| BseDI | CCNNGG | 9 cut(s) | 456, 492, 615, 918, 1162, 1357, 1657, 2158, 2284 |
| BseGI | GGATG | 8 cut(s) | 1206, 1405, 2307, 2491, 2917, 2971, 2986, 3001 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1037 |
| BseMI | GCAATG | 1 cut(s) | 1308 |
| BseNI | ACTGG | 3 cut(s) | 706, 744, 2278 |
| BseXI | GCAGC | 2 cut(s) | 143, 500 |
| Bsh1236I | CGCG | 2 cut(s) | 720, 1601 |
| Bsh1285I | CGRYCG | 1 cut(s) | 112 |
| BshFI | GGCC | 4 cut(s) | 185, 478, 1743, 2354 |
| BshVI | ATCGAT | 1 cut(s) | 2645 |
| BsiEI | CGRYCG | 1 cut(s) | 112 |
| BsiHKAI | GWGCWC | 3 cut(s) | 685, 1221, 2531 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1970, 2039, 2626 |
| BsiSI | CCGG | 5 cut(s) | 31, 314, 456, 1134, 1566 |
| BslFI | GGGAC | 4 cut(s) | 660, 1393, 1582, 2266 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1037 |
| BsmAI | GTCTC | 5 cut(s) | 763, 1157, 2081, 2159, 2943 |
| BsmBI | CGTCTC | 2 cut(s) | 763, 2943 |
| BsmFI | GGGAC | 4 cut(s) | 660, 1393, 1582, 2266 |
| BsmI | GAATGC | 3 cut(s) | 1534, 1731, 2462 |
| BsnI | GGCC | 4 cut(s) | 185, 478, 1743, 2354 |
| Bso31I | GGTCTC | 1 cut(s) | 1157 |
| BsoBI | CYCGRG | 3 cut(s) | 1970, 2039, 2626 |
| Bsp119I | TTCGAA | 2 cut(s) | 521, 2759 |
| Bsp1286I | GDGCHC | 3 cut(s) | 685, 1221, 2531 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1215 |
| Bsp19I | CCATGG | 1 cut(s) | 492 |
| BspACI | CCGC | 2 cut(s) | 112, 2721 |
| BspANI | GGCC | 4 cut(s) | 185, 478, 1743, 2354 |
| BspDI | ATCGAT | 1 cut(s) | 2645 |
| BspFNI | CGCG | 2 cut(s) | 720, 1601 |
| BspHI | TCATGA | 2 cut(s) | 997, 1690 |
| BspQI | GCTCTTC | 1 cut(s) | 673 |
| BspT104I | TTCGAA | 2 cut(s) | 521, 2759 |
| BspTI | CTTAAG | 2 cut(s) | 1049, 2837 |
| BspTNI | GGTCTC | 1 cut(s) | 1157 |
| BsrDI | GCAATG | 1 cut(s) | 1308 |
| BsrFI | RCCGGY | 1 cut(s) | 1133 |
| BsrI | ACTGG | 3 cut(s) | 706, 744, 2278 |
| BssAI | RCCGGY | 1 cut(s) | 1133 |
| BssECI | CCNNGG | 9 cut(s) | 456, 492, 615, 918, 1162, 1357, 1657, 2158, 2284 |
| BssNI | GRCGYC | 2 cut(s) | 756, 2274 |
| BssSI | CACGAG | 1 cut(s) | 2946 |
| BssT1I | CCWWGG | 6 cut(s) | 492, 615, 918, 1162, 1657, 2284 |
| Bst2BI | CACGAG | 1 cut(s) | 2946 |
| Bst2UI | CCWGG | 2 cut(s) | 1506, 2160 |
| Bst4CI | ACNGT | 5 cut(s) | 603, 651, 2512, 2861, 2896 |
| Bst6I | CTCTTC | 3 cut(s) | 673, 1188, 1482 |
| BstACI | GRCGYC | 2 cut(s) | 756, 2274 |
| BstAFI | CTTAAG | 2 cut(s) | 1049, 2837 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1130, 1453 |
| BstBAI | YACGTR | 2 cut(s) | 243, 1760 |
| BstBI | TTCGAA | 2 cut(s) | 521, 2759 |
| BstC8I | GCNNGC | 3 cut(s) | 1131, 1212, 1664 |
| BstDSI | CCRYGG | 1 cut(s) | 492 |
| BstF5I | GGATG | 8 cut(s) | 1206, 1405, 2307, 2491, 2917, 2971, 2986, 3001 |
| BstFNI | CGCG | 2 cut(s) | 720, 1601 |
| BstH2I | RGCGCY | 1 cut(s) | 107 |
| BstHHI | GCGC | 3 cut(s) | 106, 722, 1601 |
| BstMAI | GTCTC | 5 cut(s) | 763, 1157, 2081, 2159, 2943 |
| BstMCI | CGRYCG | 1 cut(s) | 112 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 1130, 1216, 1453, 2260, 2720 |
| BstNI | CCWGG | 2 cut(s) | 1506, 2160 |
| BstNSI | RCATGY | 3 cut(s) | 390, 1666, 2254 |
| BstSCI | CCNGG | 4 cut(s) | 455, 1504, 1565, 2158 |
| BstSFI | CTRYAG | 3 cut(s) | 672, 1437, 1470 |
| BstUI | CGCG | 2 cut(s) | 720, 1601 |
| BstV1I | GCAGC | 2 cut(s) | 143, 500 |
| BstX2I | RGATCY | 8 cut(s) | 201, 267, 463, 944, 1279, 1948, 2305, 2341 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 2285, 2362, 2899 |
| BstYI | RGATCY | 8 cut(s) | 201, 267, 463, 944, 1279, 1948, 2305, 2341 |
| Bsu15I | ATCGAT | 1 cut(s) | 2645 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2183 |
| BsuRI | GGCC | 4 cut(s) | 185, 478, 1743, 2354 |
| BsuTUI | ATCGAT | 1 cut(s) | 2645 |
| BtgI | CCRYGG | 1 cut(s) | 492 |
| BtgZI | GCGATG | 2 cut(s) | 384, 701 |
| BtsCI | GGATG | 8 cut(s) | 1206, 1405, 2307, 2491, 2917, 2971, 2986, 3001 |
| BtsI | GCAGTG | 1 cut(s) | 2589 |
| BtsIMutI | CAGTG | 5 cut(s) | 64, 751, 1162, 2589, 2892 |
| Cac8I | GCNNGC | 3 cut(s) | 1131, 1212, 1664 |
| CciI | TCATGA | 2 cut(s) | 997, 1690 |
| CfoI | GCGC | 3 cut(s) | 106, 722, 1601 |
| Cfr10I | RCCGGY | 1 cut(s) | 1133 |
| Cfr13I | GGNCC | 2 cut(s) | 476, 915 |
| ClaI | ATCGAT | 1 cut(s) | 2645 |
| CseI | GACGC | 3 cut(s) | 1590, 1718, 2263 |
| Csp6I | GTAC | 4 cut(s) | 669, 2044, 2573, 2943 |
| CviQI | GTAC | 4 cut(s) | 669, 2044, 2573, 2943 |
| DraI | TTTAAA | 2 cut(s) | 379, 1176 |
| DraIII | CACNNNGTG | 1 cut(s) | 246 |
| EaeI | YGGCCR | 1 cut(s) | 183 |
| Eam1104I | CTCTTC | 3 cut(s) | 673, 1188, 1482 |
| EarI | CTCTTC | 3 cut(s) | 673, 1188, 1482 |
| Ecl136II | GAGCTC | 2 cut(s) | 683, 1219 |
| Eco130I | CCWWGG | 6 cut(s) | 492, 615, 918, 1162, 1657, 2284 |
| Eco24I | GRGCYC | 2 cut(s) | 685, 1221 |
| Eco31I | GGTCTC | 1 cut(s) | 1157 |
| Eco47I | GGWCC | 1 cut(s) | 915 |
| Eco53kI | GAGCTC | 2 cut(s) | 683, 1219 |
| Eco57I | CTGAAG | 3 cut(s) | 40, 1442, 2489 |
| Eco72I | CACGTG | 1 cut(s) | 243 |
| Eco81I | CCTNAGG | 1 cut(s) | 2183 |
| Eco88I | CYCGRG | 3 cut(s) | 1970, 2039, 2626 |
| EcoICRI | GAGCTC | 2 cut(s) | 683, 1219 |
| EcoO109I | RGGNCCY | 1 cut(s) | 915 |
| EcoRII | CCWGG | 2 cut(s) | 1504, 2158 |
| EcoT14I | CCWWGG | 6 cut(s) | 492, 615, 918, 1162, 1657, 2284 |
| EcoT38I | GRGCYC | 2 cut(s) | 685, 1221 |
| ErhI | CCWWGG | 6 cut(s) | 492, 615, 918, 1162, 1657, 2284 |
| Esp3I | CGTCTC | 2 cut(s) | 763, 2943 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1650, 1682, 2834, 2866 |
| FaqI | GGGAC | 4 cut(s) | 660, 1393, 1582, 2266 |
| FauNDI | CATATG | 1 cut(s) | 2110 |
| FbaI | TGATCA | 3 cut(s) | 966, 1693, 2914 |
| FblI | GTMKAC | 2 cut(s) | 1732, 2847 |
| Fnu4HI | GCNGC | 2 cut(s) | 157, 489 |
| FokI | GGATG | 7 cut(s) | 1213, 1412, 2314, 2498, 2924, 2978, 2993 |
| FriOI | GRGCYC | 2 cut(s) | 685, 1221 |
| Fsp4HI | GCNGC | 2 cut(s) | 157, 489 |
| GlaI | GCGC | 3 cut(s) | 105, 721, 1600 |
| GluI | GCNGC | 2 cut(s) | 157, 489 |
| GsuI | CTGGAG | 1 cut(s) | 1544 |
| HaeII | RGCGCY | 1 cut(s) | 107 |
| HaeIII | GGCC | 4 cut(s) | 185, 478, 1743, 2354 |
| HapII | CCGG | 5 cut(s) | 31, 314, 456, 1134, 1566 |
| HgaI | GACGC | 3 cut(s) | 1590, 1718, 2263 |
| HhaI | GCGC | 3 cut(s) | 106, 722, 1601 |
| Hin1I | GRCGYC | 2 cut(s) | 756, 2274 |
| Hin6I | GCGC | 3 cut(s) | 104, 720, 1599 |
| HinP1I | GCGC | 3 cut(s) | 104, 720, 1599 |
| HincII | GTYRAC | 4 cut(s) | 695, 1378, 2491, 2848 |
| HindII | GTYRAC | 4 cut(s) | 695, 1378, 2491, 2848 |
| HindIII | AAGCTT | 2 cut(s) | 52, 1127 |
| HpaI | GTTAAC | 1 cut(s) | 1378 |
| HpaII | CCGG | 5 cut(s) | 31, 314, 456, 1134, 1566 |
| HphI | GGTGA | 7 cut(s) | 319, 566, 1033, 1861, 2377, 2410, 2417 |
| Hpy166II | GTNNAC | 8 cut(s) | 695, 1378, 1733, 1750, 1763, 2491, 2848, 2945 |
| Hpy8I | GTNNAC | 8 cut(s) | 695, 1378, 1733, 1750, 1763, 2491, 2848, 2945 |
| Hpy99I | CGWCG | 3 cut(s) | 44, 1518, 2129 |
| HpyAV | CCTTC | 8 cut(s) | 345, 890, 1176, 1818, 2264, 2418, 2464, 2836 |
| HpyCH4III | ACNGT | 5 cut(s) | 603, 651, 2512, 2861, 2896 |
| HpyCH4V | TGCA | 9 cut(s) | 159, 1124, 1313, 1447, 2098, 2388, 2460, 2658, 2790 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 1130, 1216, 1453, 2260, 2720 |
| Hsp92I | GRCGYC | 2 cut(s) | 756, 2274 |
| HspAI | GCGC | 3 cut(s) | 104, 720, 1599 |
| Ksp22I | TGATCA | 3 cut(s) | 966, 1693, 2914 |
| KspAI | GTTAAC | 1 cut(s) | 1378 |
| LguI | GCTCTTC | 1 cut(s) | 673 |
| LmnI | GCTCC | 4 cut(s) | 847, 1032, 1185, 1224 |
| Lsp1109I | GCAGC | 2 cut(s) | 143, 500 |
| LweI | GCATC | 6 cut(s) | 153, 1197, 1434, 2051, 2637, 2928 |
| MaeIII | GTNAC | 6 cut(s) | 278, 554, 752, 1856, 2398, 2403 |
| MfeI | CAATTG | 1 cut(s) | 2415 |
| MflI | RGATCY | 8 cut(s) | 201, 267, 463, 944, 1279, 1948, 2305, 2341 |
| MhlI | GDGCHC | 3 cut(s) | 685, 1221, 2531 |
| MlyI | GAGTC | 3 cut(s) | 1269, 1499, 2161 |
| MmeI | TCCRAC | 3 cut(s) | 544, 1310, 1377 |
| MroXI | GAANNNNTTC | 4 cut(s) | 2010, 2604, 2733, 2819 |
| MslI | CAYNNNNRTG | 2 cut(s) | 837, 2897 |
| MspCI | CTTAAG | 2 cut(s) | 1049, 2837 |
| MspI | CCGG | 5 cut(s) | 31, 314, 456, 1134, 1566 |
| MspR9I | CCNGG | 4 cut(s) | 457, 1506, 1567, 2160 |
| MunI | CAATTG | 1 cut(s) | 2415 |
| Mva1269I | GAATGC | 3 cut(s) | 1534, 1731, 2462 |
| MvaI | CCWGG | 2 cut(s) | 1506, 2160 |
| MvnI | CGCG | 2 cut(s) | 720, 1601 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 1130, 1216, 1453, 2260, 2720 |
| NciI | CCSGG | 2 cut(s) | 457, 1567 |
| NcoI | CCATGG | 1 cut(s) | 492 |
| NdeI | CATATG | 1 cut(s) | 2110 |
| NmuCI | GTSAC | 5 cut(s) | 278, 554, 752, 1856, 2398 |
| NspI | RCATGY | 3 cut(s) | 390, 1666, 2254 |
| NspV | TTCGAA | 2 cut(s) | 521, 2759 |
| OliI | CACNNNNGTG | 1 cut(s) | 2897 |
| PaeI | GCATGC | 1 cut(s) | 1666 |
| PaeR7I | CTCGAG | 3 cut(s) | 1970, 2039, 2626 |
| PagI | TCATGA | 2 cut(s) | 997, 1690 |
| PciSI | GCTCTTC | 1 cut(s) | 673 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 105, 1554, 2046 |
| PctI | GAATGC | 3 cut(s) | 1534, 1731, 2462 |
| PdmI | GAANNNNTTC | 4 cut(s) | 2010, 2604, 2733, 2819 |
| PfeI | GAWTC | 7 cut(s) | 310, 611, 1610, 1912, 2033, 2422, 2888 |
| PflFI | GACNNNGTC | 1 cut(s) | 560 |
| PfoI | TCCNGGA | 1 cut(s) | 1504 |
| PkrI | GCNGC | 2 cut(s) | 158, 490 |
| PleI | GAGTC | 3 cut(s) | 1268, 1498, 2160 |
| PmaCI | CACGTG | 1 cut(s) | 243 |
| PmlI | CACGTG | 1 cut(s) | 243 |
| PpsI | GAGTC | 3 cut(s) | 1268, 1498, 2160 |
| Ppu21I | YACGTR | 2 cut(s) | 243, 1760 |
| PpuMI | RGGWCCY | 1 cut(s) | 915 |
| PsiI | TTATAA | 2 cut(s) | 179, 735 |
| Psp124BI | GAGCTC | 2 cut(s) | 685, 1221 |
| Psp1406I | AACGTT | 2 cut(s) | 80, 1394 |
| Psp5II | RGGWCCY | 1 cut(s) | 915 |
| Psp6I | CCWGG | 2 cut(s) | 1504, 2158 |
| PspCI | CACGTG | 1 cut(s) | 243 |
| PspGI | CCWGG | 2 cut(s) | 1504, 2158 |
| PspPI | GGNCC | 2 cut(s) | 476, 915 |
| PspPPI | RGGWCCY | 1 cut(s) | 915 |
| PsuI | RGATCY | 8 cut(s) | 201, 267, 463, 944, 1279, 1948, 2305, 2341 |
| PsyI | GACNNNGTC | 1 cut(s) | 560 |
| RsaI | GTAC | 4 cut(s) | 670, 2045, 2574, 2944 |
| RsaNI | GTAC | 4 cut(s) | 669, 2044, 2573, 2943 |
| RseI | CAYNNNNRTG | 2 cut(s) | 837, 2897 |
| SacI | GAGCTC | 2 cut(s) | 685, 1221 |
| SalI | GTCGAC | 1 cut(s) | 2846 |
| SapI | GCTCTTC | 1 cut(s) | 673 |
| SatI | GCNGC | 2 cut(s) | 157, 489 |
| Sau96I | GGNCC | 2 cut(s) | 476, 915 |
| ScaI | AGTACT | 1 cut(s) | 670 |
| SchI | GAGTC | 3 cut(s) | 1269, 1499, 2161 |
| ScrFI | CCNGG | 4 cut(s) | 457, 1506, 1567, 2160 |
| SduI | GDGCHC | 3 cut(s) | 685, 1221, 2531 |
| SfaNI | GCATC | 6 cut(s) | 153, 1197, 1434, 2051, 2637, 2928 |
| SfcI | CTRYAG | 3 cut(s) | 672, 1437, 1470 |
| Sfr274I | CTCGAG | 3 cut(s) | 1970, 2039, 2626 |
| SfuI | TTCGAA | 2 cut(s) | 521, 2759 |
| SinI | GGWCC | 1 cut(s) | 915 |
| SlaI | CTCGAG | 3 cut(s) | 1970, 2039, 2626 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 837, 2897 |
| SpeI | ACTAGT | 1 cut(s) | 2690 |
| SphI | GCATGC | 1 cut(s) | 1666 |
| SsiI | CCGC | 2 cut(s) | 112, 2721 |
| SspI | AATATT | 1 cut(s) | 1931 |
| SstI | GAGCTC | 2 cut(s) | 685, 1221 |
| StyD4I | CCNGG | 4 cut(s) | 455, 1504, 1565, 2158 |
| StyI | CCWWGG | 6 cut(s) | 492, 615, 918, 1162, 1657, 2284 |
| TaaI | ACNGT | 5 cut(s) | 603, 651, 2512, 2861, 2896 |
| TatI | WGTACW | 2 cut(s) | 668, 2572 |
| TfiI | GAWTC | 7 cut(s) | 310, 611, 1610, 1912, 2033, 2422, 2888 |
| TscAI | CASTG | 5 cut(s) | 64, 751, 1162, 2596, 2899 |
| TseFI | GTSAC | 5 cut(s) | 278, 554, 752, 1856, 2398 |
| TseI | GCWGC | 2 cut(s) | 156, 488 |
| Tsp45I | GTSAC | 5 cut(s) | 278, 554, 752, 1856, 2398 |
| TspRI | CASTG | 5 cut(s) | 64, 751, 1162, 2596, 2899 |
| Tth111I | GACNNNGTC | 1 cut(s) | 560 |
| Vha464I | CTTAAG | 2 cut(s) | 1049, 2837 |
| VpaK11BI | GGWCC | 1 cut(s) | 915 |
| XbaI | TCTAGA | 3 cut(s) | 901, 1543, 2581 |
| XceI | RCATGY | 3 cut(s) | 390, 1666, 2254 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1070 |
| XhoI | CTCGAG | 3 cut(s) | 1970, 2039, 2626 |
| XmiI | GTMKAC | 2 cut(s) | 1732, 2847 |
| XmnI | GAANNNNTTC | 4 cut(s) | 2010, 2604, 2733, 2819 |
| ZraI | GACGTC | 1 cut(s) | 757 |
| ZrmI | AGTACT | 1 cut(s) | 670 |