AT4G19350

Nnf1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010244
Physical Location & Seq
Reverse (-)
10561950 .. 10563757
1808 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G19350.1

Sequence Viewer

Length: 543 bp
ATGGAGAAACCGGGTCATGAAACGGATATTGCGGGTTCAAGACGAACCCATCTGAAGAAATCATTCAAATCCACTCTTCGACATCTTCTCACTGCTTGCTCCAAACAGGATTTTGTTGATATTTTCTCGAAATTCAGTGGGGCTGAGCAAGAACTGCTTTTTCAGTTGTATACTCGGGTTGTTGTGAATTTGCATCAGACAATAGAGGAAGAGTTTGATGAACAATGTCACGAAACTCAGGTCGGACCAATTCTTGATACAGTCGAGGAGCTGGTCGAAGAACAGAGTTTGGATCCCTTGTTTTCTGATAAGACTGATGTAATGGCAATAGCATATGATCTTACAACATCTAAGAAGAACGAGATACAAAAGTTAACAGTTTTGCTCCAAAGGGCTGAAGAACAGAATCGCCAGAAGGAAGCCCGGATCAGCATGCTCAAAAAACAAACACAAGATCTCTCAGGCACAGCGGACAGAATCGAAAAGCTGAATGCCGGGGTCTCCGGCTACTTTGAAGGAAAAGACAAGCTACCTCCAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nnf1

Protein Analysis

180

Amino Acids

20.54

Weight (kDa)

5.32

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nnf1 PF03980 55 - 147 6.7e-06 Nnf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 170
AciI CCGC 2 cut(s) 32, 470
AclWI GGATC 3 cut(s) 287, 300, 434
AcsI RAATTY 2 cut(s) 131, 187
AcuI CTGAAG 2 cut(s) 74, 417
AgsI TTSAA 3 cut(s) 39, 67, 515
AluBI AGCT 3 cut(s) 271, 487, 529
AluI AGCT 3 cut(s) 271, 487, 529
Alw26I GTCTC 1 cut(s) 505
AlwI GGATC 3 cut(s) 287, 300, 434
Ama87I CYCGRG 1 cut(s) 174
ApoI RAATTY 2 cut(s) 131, 187
ArsI GACNNNNNNTTYG 2 cut(s) 225, 257
Asp700I GAANNNNTTC 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 3 cut(s) 12, 424, 496
AvaI CYCGRG 1 cut(s) 174
AvaII GGWCC 1 cut(s) 245
BaeI ACNNNNGTAYC 2 cut(s) 356, 389
BamHI GGATCC 1 cut(s) 292
BccI CCATC 1 cut(s) 57
BcnI CCSGG 3 cut(s) 12, 424, 496
BcoDI GTCTC 1 cut(s) 505
BglII AGATCT 1 cut(s) 454
BlpI GCTNAGC 1 cut(s) 144
Bme1390I CCNGG 3 cut(s) 12, 424, 496
Bme18I GGWCC 1 cut(s) 245
BmeT110I CYCGRG 1 cut(s) 174
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 1 cut(s) 294
BmrFI CCNGG 3 cut(s) 12, 424, 496
BmsI GCATC 1 cut(s) 202
Bpu1102I GCTNAGC 1 cut(s) 144
BpuMI CCSGG 3 cut(s) 12, 424, 496
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 1 cut(s) 495
BseDI CCNNGG 1 cut(s) 495
BseMII CTCAG 3 cut(s) 135, 251, 474
BseRI GAGGAG 1 cut(s) 281
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 4 cut(s) 11, 424, 495, 504
BsmAI GTCTC 1 cut(s) 505
BsmI GAATGC 1 cut(s) 496
Bso31I GGTCTC 1 cut(s) 505
BsoBI CYCGRG 1 cut(s) 174
Bsp143I GATC 4 cut(s) 292, 337, 426, 454
Bsp1720I GCTNAGC 1 cut(s) 144
BspACI CCGC 2 cut(s) 32, 470
BspCNI CTCAG 3 cut(s) 136, 250, 473
BspHI TCATGA 1 cut(s) 16
BspLI GGNNCC 1 cut(s) 294
BspPI GGATC 3 cut(s) 287, 300, 434
BspTNI GGTCTC 1 cut(s) 505
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 4 cut(s) 292, 337, 426, 454
BssNAI GTATAC 1 cut(s) 171
Bst1107I GTATAC 1 cut(s) 171
Bst4CI ACNGT 2 cut(s) 262, 379
Bst6I CTCTTC 2 cut(s) 81, 204
BstAPI GCANNNNNTGC 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 97, 434
BstDEI CTNAG 4 cut(s) 144, 237, 351, 460
BstKTI GATC 4 cut(s) 295, 340, 429, 457
BstMAI GTCTC 1 cut(s) 505
BstMBI GATC 4 cut(s) 292, 337, 426, 454
BstMWI GCNNNNNNNGC 1 cut(s) 154
BstNSI RCATGY 1 cut(s) 436
BstSCI CCNGG 3 cut(s) 10, 422, 494
BstX2I RGATCY 2 cut(s) 292, 454
BstYI RGATCY 2 cut(s) 292, 454
BstZ17I GTATAC 1 cut(s) 171
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 2 cut(s) 90, 142
Cac8I GCNNGC 2 cut(s) 97, 434
CciI TCATGA 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 245
CviAII CATG 2 cut(s) 17, 433
CviJI RGCY 7 cut(s) 143, 271, 395, 422, 487, 507, 529
CviKI_1 RGCY 7 cut(s) 143, 271, 395, 422, 487, 507, 529
DdeI CTNAG 4 cut(s) 144, 237, 351, 460
DpnI GATC 4 cut(s) 294, 339, 428, 456
DpnII GATC 4 cut(s) 292, 337, 426, 454
Eam1104I CTCTTC 2 cut(s) 81, 204
EarI CTCTTC 2 cut(s) 81, 204
Eco31I GGTCTC 1 cut(s) 505
Eco47I GGWCC 1 cut(s) 245
Eco57I CTGAAG 2 cut(s) 74, 417
Eco88I CYCGRG 1 cut(s) 174
FaeI CATG 2 cut(s) 20, 436
FaiI YATR 6 cut(s) 18, 171, 334, 336, 434, 541
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FatI CATG 2 cut(s) 16, 432
FauI CCCGC 1 cut(s) 25
FauNDI CATATG 1 cut(s) 334
FblI GTMKAC 1 cut(s) 170
HapII CCGG 4 cut(s) 11, 424, 495, 504
Hin1II CATG 2 cut(s) 20, 436
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HinfI GANTC 2 cut(s) 406, 477
HpaI GTTAAC 1 cut(s) 375
HpaII CCGG 4 cut(s) 11, 424, 495, 504
Hpy166II GTNNAC 2 cut(s) 171, 375
Hpy188I TCNGA 4 cut(s) 54, 198, 245, 307
Hpy188III TCNNGA 5 cut(s) 17, 39, 127, 230, 254
Hpy8I GTNNAC 2 cut(s) 171, 375
HpyAV CCTTC 2 cut(s) 409, 509
HpyCH4III ACNGT 2 cut(s) 262, 379
HpyCH4V TGCA 1 cut(s) 193
HpyF10VI GCNNNNNNNGC 1 cut(s) 154
HpyF3I CTNAG 4 cut(s) 144, 237, 351, 460
Hsp92II CATG 2 cut(s) 20, 436
KspAI GTTAAC 1 cut(s) 375
Kzo9I GATC 4 cut(s) 292, 337, 426, 454
LmnI GCTCC 3 cut(s) 104, 268, 390
LpnPI CCDG 9 cut(s) 24, 92, 224, 257, 425, 437, 447, 508, 517
LweI GCATC 1 cut(s) 202
MaeIII GTNAC 1 cut(s) 227
MalI GATC 4 cut(s) 294, 339, 428, 456
MboI GATC 4 cut(s) 292, 337, 426, 454
MboII GAAGA 7 cut(s) 67, 68, 77, 221, 290, 367, 410
MflI RGATCY 2 cut(s) 292, 454
MluCI AATT 3 cut(s) 131, 187, 249
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 3 cut(s) 199, 259, 543
MroXI GAANNNNTTC 1 cut(s) 62
MseI TTAA 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 4 cut(s) 11, 424, 495, 504
MspR9I CCNGG 3 cut(s) 12, 424, 496
Mva1269I GAATGC 1 cut(s) 496
MwoI GCNNNNNNNGC 1 cut(s) 154
NciI CCSGG 3 cut(s) 12, 424, 496
NdeI CATATG 1 cut(s) 334
NdeII GATC 4 cut(s) 292, 337, 426, 454
NlaIII CATG 2 cut(s) 20, 436
NlaIV GGNNCC 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 227
NspI RCATGY 1 cut(s) 436
PaeI GCATGC 1 cut(s) 436
PagI TCATGA 1 cut(s) 16
PctI GAATGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 2 cut(s) 406, 477
PspN4I GGNNCC 1 cut(s) 294
PspPI GGNCC 1 cut(s) 245
PsuI RGATCY 2 cut(s) 292, 454
SaqAI TTAA 1 cut(s) 374
Sau3AI GATC 4 cut(s) 292, 337, 426, 454
Sau96I GGNCC 1 cut(s) 245
ScrFI CCNGG 3 cut(s) 12, 424, 496
SetI ASST 5 cut(s) 243, 273, 489, 531, 535
SfaNI GCATC 1 cut(s) 202
SinI GGWCC 1 cut(s) 245
SphI GCATGC 1 cut(s) 436
Sse9I AATT 3 cut(s) 131, 187, 249
SsiI CCGC 2 cut(s) 32, 470
StyD4I CCNGG 3 cut(s) 10, 422, 494
TaaI ACNGT 2 cut(s) 262, 379
TaqI TCGA 5 cut(s) 79, 128, 264, 276, 480
TasI AATT 3 cut(s) 131, 187, 249
TfiI GAWTC 2 cut(s) 406, 477
Tru1I TTAA 1 cut(s) 374
Tru9I TTAA 1 cut(s) 374
TscAI CASTG 2 cut(s) 97, 142
TseFI GTSAC 1 cut(s) 227
Tsp45I GTSAC 1 cut(s) 227
TspDTI ATGAA 2 cut(s) 33, 234
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 2 cut(s) 97, 142
VpaK11BI GGWCC 1 cut(s) 245
XapI RAATTY 2 cut(s) 131, 187
XceI RCATGY 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 170
XmnI GAANNNNTTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.