Rh7AG289000

Nnf1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0010244
Physical Location & Seq
Forward (+)
31208739 .. 31211575
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG289000.1

Sequence Viewer

Length: 477 bp
ATGGGGACGAGGCAAAGGGACTTGAACAAGTCCTTCAACCTCGCCATACGTTCTCTCCTCGCTCCTTGCTCTTTTCAGGAATTCTGTGAAGCGCTGCCGAGTTTCACAGCTGCTGAGCATGAACGTCTTCACAGGCTCTTTACTCAGGTCATGAGTTCTTTGCATGGGAATGTAGAGGACGAGTTTCAGCATATATGTCTCGAAACACAGGTTGGAACTGCAATGGATACTGTAGAGCAACTGGTGGAAGAACAAAGTCTGGATCCTTTGTTTTCAAATAAGACTAATGTAATGGATGTTGCCCACGATCTGTCAAGAACAACAAAGAAGGAGATCCAGTACTTGACAGAGATGCTTGAAAAGGTACACAACTACACATATACAAAATTAAACTTCATTTTAAACTCTTTAAGAGAGAGCAGAGACAGCATTGTCCACTTCTCCGGGTATTTTCCAACATTTACTGACATGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nnf1

Protein Analysis

158

Amino Acids

18.3

Weight (kDa)

5.29

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nnf1 PF03980 43 - 124 6.3e-07 Nnf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 431
AclWI GGATC 3 cut(s) 257, 270, 328
AcsI RAATTY 1 cut(s) 80
AfaI GTAC 2 cut(s) 341, 366
AfeI AGCGCT 1 cut(s) 93
AgsI TTSAA 4 cut(s) 25, 37, 276, 359
AjuI GAANNNNNNNTTGG 2 cut(s) 195, 227
AluBI AGCT 1 cut(s) 110
AluI AGCT 1 cut(s) 110
Alw26I GTCTC 2 cut(s) 203, 417
AlwI GGATC 3 cut(s) 257, 270, 328
AlwNI CAGNNNCTG 1 cut(s) 113
Aor51HI AGCGCT 1 cut(s) 93
ApeKI GCWGC 2 cut(s) 94, 110
ApoI RAATTY 1 cut(s) 80
Asp700I GAANNNNTTC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 94
AsuC2I CCSGG 1 cut(s) 445
BamHI GGATCC 1 cut(s) 262
BbsI GAAGAC 1 cut(s) 119
BbvI GCAGC 2 cut(s) 81, 97
BciVI GTATCC 1 cut(s) 220
BcnI CCSGG 1 cut(s) 445
BcoDI GTCTC 2 cut(s) 203, 417
BfmI CTRYAG 1 cut(s) 231
BfoI RGCGCY 1 cut(s) 95
BfuI GTATCC 1 cut(s) 220
BisI GCNGC 2 cut(s) 95, 111
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 2 cut(s) 96, 112
BmcAI AGTACT 1 cut(s) 341
Bme1390I CCNGG 1 cut(s) 445
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 1 cut(s) 445
BmsI GCATC 1 cut(s) 342
BpiI GAAGAC 1 cut(s) 119
Bpu1102I GCTNAGC 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 445
BsaXI ACNNNNNCTCC 4 cut(s) 39, 69, 323, 353
Bse1I ACTGG 2 cut(s) 246, 337
Bse3DI GCAATG 1 cut(s) 228
BseGI GGATG 1 cut(s) 301
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 105, 158
BseNI ACTGG 2 cut(s) 246, 337
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 2 cut(s) 81, 97
BsiSI CCGG 1 cut(s) 444
BslFI GGGAC 2 cut(s) 19, 32
BsmAI GTCTC 2 cut(s) 203, 417
BsmFI GGGAC 2 cut(s) 19, 32
Bsp143I GATC 3 cut(s) 262, 307, 333
Bsp1720I GCTNAGC 1 cut(s) 114
BspCNI CTCAG 2 cut(s) 106, 157
BspHI TCATGA 1 cut(s) 150
BspLI GGNNCC 1 cut(s) 264
BspPI GGATC 3 cut(s) 257, 270, 328
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 2 cut(s) 246, 337
BssMI GATC 3 cut(s) 262, 307, 333
Bst4CI ACNGT 1 cut(s) 232
BstDEI CTNAG 2 cut(s) 114, 144
BstF5I GGATG 1 cut(s) 301
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 3 cut(s) 265, 310, 336
BstMAI GTCTC 2 cut(s) 203, 417
BstMBI GATC 3 cut(s) 262, 307, 333
BstMWI GCNNNNNNNGC 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 443
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 81, 97
BstV2I GAAGAC 1 cut(s) 119
BstX2I RGATCY 2 cut(s) 262, 333
BstYI RGATCY 2 cut(s) 262, 333
BsuI GTATCC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 301
CaiI CAGNNNCTG 1 cut(s) 113
CciI TCATGA 1 cut(s) 150
CfoI GCGC 1 cut(s) 94
Csp6I GTAC 2 cut(s) 340, 365
CviAII CATG 4 cut(s) 119, 151, 164, 469
CviJI RGCY 2 cut(s) 110, 136
CviKI_1 RGCY 2 cut(s) 110, 136
CviQI GTAC 2 cut(s) 340, 365
DdeI CTNAG 2 cut(s) 114, 144
DpnI GATC 3 cut(s) 264, 309, 335
DpnII GATC 3 cut(s) 262, 307, 333
DraI TTTAAA 1 cut(s) 402
DrdI GACNNNNNNGTC 1 cut(s) 431
DseDI GACNNNNNNGTC 1 cut(s) 431
Eco47III AGCGCT 1 cut(s) 93
EcoRI GAATTC 1 cut(s) 80
FaeI CATG 4 cut(s) 122, 154, 167, 472
FaqI GGGAC 2 cut(s) 19, 32
FatI CATG 4 cut(s) 118, 150, 163, 468
Fnu4HI GCNGC 2 cut(s) 95, 111
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 2 cut(s) 95, 111
GlaI GCGC 1 cut(s) 93
GluI GCNGC 2 cut(s) 95, 111
HaeII RGCGCY 1 cut(s) 95
HapII CCGG 1 cut(s) 444
HhaI GCGC 1 cut(s) 94
Hin1II CATG 4 cut(s) 122, 154, 167, 472
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HpaII CCGG 1 cut(s) 444
Hpy166II GTNNAC 2 cut(s) 367, 436
Hpy188III TCNNGA 5 cut(s) 77, 151, 200, 260, 315
Hpy8I GTNNAC 2 cut(s) 367, 436
HpyAV CCTTC 2 cut(s) 43, 322
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 2 cut(s) 49, 124
HpyCH4V TGCA 2 cut(s) 163, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 426
HpyF3I CTNAG 2 cut(s) 114, 144
HpySE526I ACGT 2 cut(s) 49, 124
Hsp92II CATG 4 cut(s) 122, 154, 167, 472
HspAI GCGC 1 cut(s) 92
Kzo9I GATC 3 cut(s) 262, 307, 333
LmnI GCTCC 1 cut(s) 67
LpnPI CCDG 8 cut(s) 62, 118, 131, 194, 227, 245, 350, 457
Lsp1109I GCAGC 2 cut(s) 81, 97
LweI GCATC 1 cut(s) 342
MaeII ACGT 2 cut(s) 49, 124
MalI GATC 3 cut(s) 264, 309, 335
MboI GATC 3 cut(s) 262, 307, 333
MboII GAAGA 2 cut(s) 119, 260
MflI RGATCY 2 cut(s) 262, 333
MluCI AATT 2 cut(s) 80, 386
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 4 cut(s) 3, 50, 68, 169
MroXI GAANNNNTTC 1 cut(s) 126
MseI TTAA 3 cut(s) 389, 401, 410
MslI CAYNNNNRTG 1 cut(s) 168
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 1 cut(s) 444
MspR9I CCNGG 1 cut(s) 445
MwoI GCNNNNNNNGC 1 cut(s) 426
NciI CCSGG 1 cut(s) 445
NdeII GATC 3 cut(s) 262, 307, 333
NlaIII CATG 4 cut(s) 122, 154, 167, 472
NlaIV GGNNCC 1 cut(s) 264
NmeAIII GCCGAG 1 cut(s) 123
PagI TCATGA 1 cut(s) 150
PdmI GAANNNNTTC 1 cut(s) 126
PkrI GCNGC 2 cut(s) 96, 112
PspN4I GGNNCC 1 cut(s) 264
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 2 cut(s) 262, 333
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 2 cut(s) 341, 366
RsaNI GTAC 2 cut(s) 340, 365
RseI CAYNNNNRTG 1 cut(s) 168
SaqAI TTAA 3 cut(s) 389, 401, 410
SatI GCNGC 2 cut(s) 95, 111
Sau3AI GATC 3 cut(s) 262, 307, 333
ScaI AGTACT 1 cut(s) 341
ScrFI CCNGG 1 cut(s) 445
SetI ASST 7 cut(s) 42, 52, 112, 127, 150, 213, 366
SfaNI GCATC 1 cut(s) 342
SfcI CTRYAG 1 cut(s) 231
SmiMI CAYNNNNRTG 1 cut(s) 168
Sse9I AATT 2 cut(s) 80, 386
StyD4I CCNGG 1 cut(s) 443
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 2 cut(s) 52, 127
TaqI TCGA 1 cut(s) 201
TasI AATT 2 cut(s) 80, 386
TatI WGTACW 1 cut(s) 339
Tru1I TTAA 3 cut(s) 389, 401, 410
Tru9I TTAA 3 cut(s) 389, 401, 410
TseI GCWGC 2 cut(s) 94, 110
TspDTI ATGAA 2 cut(s) 135, 385
XapI RAATTY 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 126
ZrmI AGTACT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.