Rorug07G0152400

Nnf1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010244
Physical Location & Seq
Reverse (-)
12079262 .. 12079705
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0152400.1

Sequence Viewer

Length: 444 bp
ATGGATGTGGATGTACTCAGCTGGAATTGCCGAGGAATATGTAATGAGGCAACGACAAGGGCATTGAAGGACTTGATTACTCAAAATCGACCTCAAATTGTGTTTTTATGTGAAACGAAAATCAGTCGTCAGGAGGAGTTTCACAAACTGCATCAGGCTCTAGGGTTCGCTCATGCTGAATCGGTTCTGAGCGAGGGACAGTCTGGTGGATTGGCGTTGTTCTGGAATGATGAGATTAACCTGCAGATGCGAACATCATCGGCCCATCATATGGACGCTGTGATTGTGGGGGATTCTGGTGCTCTTTTATGGAGGATGACTGGTTTTTATGGGTATGCAAGAACTAGGGATCGTGAGCGTTCCTGGCAGCTTTTCCGGGACCTTTCGGATCTAGACTCGCTACCATGGATTGTGGTTGGGGATTTTAATGAGATTTTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nnf1

Protein Analysis

147

Amino Acids

16.83

Weight (kDa)

5.47

Isoelectric Point (pI)

34.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 6 - 144 1.3e-14 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 249
AccB7I CCANNNNNTGG 1 cut(s) 271
AclWI GGATC 2 cut(s) 357, 396
AfaI GTAC 1 cut(s) 15
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 2 cut(s) 67, 439
AjnI CCWGG 1 cut(s) 362
AluBI AGCT 2 cut(s) 21, 370
AluI AGCT 2 cut(s) 21, 370
Alw21I GWGCWC 1 cut(s) 304
AlwI GGATC 2 cut(s) 357, 396
AoxI GGCC 1 cut(s) 261
ApeKI GCWGC 1 cut(s) 367
Asp700I GAANNNNTTC 1 cut(s) 183
AspS9I GGNCC 2 cut(s) 262, 379
AsuC2I CCSGG 1 cut(s) 377
AvaII GGWCC 1 cut(s) 379
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 379
BccI CCATC 1 cut(s) 273
BciT130I CCWGG 1 cut(s) 364
BcnI CCSGG 1 cut(s) 377
BfaI CTAG 3 cut(s) 161, 345, 392
BfmI CTRYAG 1 cut(s) 242
BfuAI ACCTGC 1 cut(s) 249
BisI GCNGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 369
Bme1390I CCNGG 2 cut(s) 364, 377
Bme18I GGWCC 1 cut(s) 379
BmgT120I GGNCC 2 cut(s) 262, 379
BmiI GGNNCC 1 cut(s) 380
BmrFI CCNGG 2 cut(s) 364, 377
BmsI GCATC 2 cut(s) 160, 237
BpuMI CCSGG 1 cut(s) 377
BsaJI CCNNGG 2 cut(s) 31, 404
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse1I ACTGG 1 cut(s) 325
BseBI CCWGG 1 cut(s) 364
BseDI CCNNGG 2 cut(s) 31, 404
BseGI GGATG 3 cut(s) 10, 16, 321
BseLI CCNNNNNNNGG 1 cut(s) 271
BseMII CTCAG 2 cut(s) 31, 179
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 1 cut(s) 149
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 304
BsiSI CCGG 1 cut(s) 376
BslFI GGGAC 2 cut(s) 210, 392
BslI CCNNNNNNNGG 1 cut(s) 271
BsmFI GGGAC 2 cut(s) 210, 392
BsnI GGCC 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 2 cut(s) 349, 388
Bsp19I CCATGG 1 cut(s) 404
BspANI GGCC 1 cut(s) 263
BspCNI CTCAG 2 cut(s) 30, 180
BspLI GGNNCC 1 cut(s) 380
BspMAI CTGCAG 1 cut(s) 246
BspMI ACCTGC 1 cut(s) 249
BspPI GGATC 2 cut(s) 357, 396
BsrI ACTGG 1 cut(s) 325
BssECI CCNNGG 2 cut(s) 31, 404
BssMI GATC 2 cut(s) 349, 388
BssT1I CCWWGG 1 cut(s) 404
Bst2UI CCWGG 1 cut(s) 364
Bst4CI ACNGT 1 cut(s) 201
BstDEI CTNAG 2 cut(s) 17, 188
BstDSI CCRYGG 1 cut(s) 404
BstF5I GGATG 3 cut(s) 10, 16, 321
BstKTI GATC 2 cut(s) 352, 391
BstMBI GATC 2 cut(s) 349, 388
BstMWI GCNNNNNNNGC 2 cut(s) 27, 364
BstNI CCWGG 1 cut(s) 364
BstSCI CCNGG 2 cut(s) 362, 375
BstSFI CTRYAG 1 cut(s) 242
BstV1I GCAGC 1 cut(s) 379
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 1 cut(s) 263
BtgI CCRYGG 1 cut(s) 404
BtsCI GGATG 3 cut(s) 10, 16, 321
BveI ACCTGC 1 cut(s) 249
Cfr13I GGNCC 2 cut(s) 262, 379
CseI GACGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 14
CviAII CATG 2 cut(s) 173, 405
CviJI RGCY 4 cut(s) 21, 158, 263, 370
CviKI_1 RGCY 4 cut(s) 21, 158, 263, 370
CviQI GTAC 1 cut(s) 14
DdeI CTNAG 2 cut(s) 17, 188
DpnI GATC 2 cut(s) 351, 390
DpnII GATC 2 cut(s) 349, 388
Eco130I CCWWGG 1 cut(s) 404
Eco47I GGWCC 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 362
EcoT14I CCWWGG 1 cut(s) 404
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 2 cut(s) 176, 408
FaiI YATR 9 cut(s) 40, 109, 174, 270, 272, 310, 330, 336, 406
FaqI GGGAC 2 cut(s) 210, 392
FatI CATG 2 cut(s) 172, 404
FauNDI CATATG 1 cut(s) 270
Fnu4HI GCNGC 1 cut(s) 368
FokI GGATG 3 cut(s) 17, 23, 328
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 3 cut(s) 161, 345, 392
GluI GCNGC 1 cut(s) 368
HaeIII GGCC 1 cut(s) 263
HapII CCGG 1 cut(s) 376
HgaI GACGC 1 cut(s) 284
Hin1II CATG 2 cut(s) 176, 408
HinfI GANTC 3 cut(s) 179, 293, 395
HpaII CCGG 1 cut(s) 376
Hpy188I TCNGA 2 cut(s) 189, 388
Hpy188III TCNNGA 4 cut(s) 131, 223, 353, 392
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 1 cut(s) 201
HpyCH4V TGCA 3 cut(s) 151, 244, 338
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 364
HpyF3I CTNAG 2 cut(s) 17, 188
Hsp92II CATG 2 cut(s) 176, 408
Kzo9I GATC 2 cut(s) 349, 388
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 2 cut(s) 160, 237
MaeI CTAG 3 cut(s) 161, 345, 392
MalI GATC 2 cut(s) 351, 390
MboI GATC 2 cut(s) 349, 388
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 2 cut(s) 25, 96
MlyI GAGTC 1 cut(s) 389
MnlI CCTC 6 cut(s) 26, 40, 102, 127, 187, 306
MroXI GAANNNNTTC 1 cut(s) 183
MseI TTAA 2 cut(s) 237, 426
MspA1I CMGCKG 1 cut(s) 21
MspI CCGG 1 cut(s) 376
MspR9I CCNGG 2 cut(s) 364, 377
MvaI CCWGG 1 cut(s) 364
MwoI GCNNNNNNNGC 2 cut(s) 27, 364
NciI CCSGG 1 cut(s) 377
NcoI CCATGG 1 cut(s) 404
NdeI CATATG 1 cut(s) 270
NdeII GATC 2 cut(s) 349, 388
NlaIII CATG 2 cut(s) 176, 408
NlaIV GGNNCC 1 cut(s) 380
NmeAIII GCCGAG 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 2 cut(s) 179, 293
PflMI CCANNNNNTGG 1 cut(s) 271
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 1 cut(s) 369
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
PpuMI RGGWCCY 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 362
PspGI CCWGG 1 cut(s) 362
PspN4I GGNNCC 1 cut(s) 380
PspPI GGNCC 2 cut(s) 262, 379
PspPPI RGGWCCY 1 cut(s) 379
PstI CTGCAG 1 cut(s) 246
PsuI RGATCY 1 cut(s) 388
PvuII CAGCTG 1 cut(s) 21
RsaI GTAC 1 cut(s) 15
RsaNI GTAC 1 cut(s) 14
SaqAI TTAA 2 cut(s) 237, 426
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 2 cut(s) 349, 388
Sau96I GGNCC 2 cut(s) 262, 379
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 364, 377
SduI GDGCHC 1 cut(s) 304
SetI ASST 5 cut(s) 23, 94, 243, 372, 384
SfaNI GCATC 2 cut(s) 160, 237
SfcI CTRYAG 1 cut(s) 242
SinI GGWCC 1 cut(s) 379
Sse9I AATT 2 cut(s) 25, 96
SspMI CTAG 3 cut(s) 161, 345, 392
StyD4I CCNGG 2 cut(s) 362, 375
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 201
TaqI TCGA 1 cut(s) 88
TasI AATT 2 cut(s) 25, 96
TatI WGTACW 1 cut(s) 13
TfiI GAWTC 2 cut(s) 179, 293
Tru1I TTAA 2 cut(s) 237, 426
Tru9I TTAA 2 cut(s) 237, 426
TseI GCWGC 1 cut(s) 367
Van91I CCANNNNNTGG 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 379
XbaI TCTAGA 1 cut(s) 391
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 3 cut(s) 161, 345, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.