Rh7BG281000

Nnf1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010244
Physical Location & Seq
Forward (+)
26210223 .. 26214672
4450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG281000.1

Sequence Viewer

Length: 477 bp
ATGGGGACGAGGCAAAGGGACTTGAACAAGTCCTTCAACCTCGCCATACGTTCTCTGCTCGCTCCCTGCTCTTTTCAGGAATTCTGTGAAGCGCTGCCGAGTTTCACAGCTGCTGAGCATGAACGTCTTCACAGGCTCTTTACTCAGGTCATGAGCTCTTTGCATGGGAATGTAGAGGACGAGTTTCAGCATATATGTCTCGAAACACAGGTTGGAACTGCAATGGATACTGTAGAGCAACTGGTGGAAGAACAAAGTCTGGATCCTTTGTTTTCAAATAAGACTAATGTAATGGATGTTGCTCACGATCTGTCAAGAACAACAAAGAAGGAGATCCAGTACTTGACAGAGATGCTTGAAAAGGTACACAACTACACATATACAAAATTAAACTTCATTTTAAACTTCTTAAGAGATAGCAGAGACAGCATTGTCTACTTCTCCAGGTATTTTCCAACATTTACTGACATGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nnf1

Protein Analysis

158

Amino Acids

18.47

Weight (kDa)

5.31

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nnf1 PF03980 43 - 124 7.6e-07 Nnf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 431
AccI GTMKAC 1 cut(s) 435
AclWI GGATC 3 cut(s) 257, 270, 328
AcsI RAATTY 1 cut(s) 80
AfaI GTAC 2 cut(s) 341, 366
AfeI AGCGCT 1 cut(s) 93
AflII CTTAAG 1 cut(s) 409
AgsI TTSAA 4 cut(s) 25, 37, 276, 359
AjnI CCWGG 1 cut(s) 443
AjuI GAANNNNNNNTTGG 2 cut(s) 195, 227
AluBI AGCT 2 cut(s) 110, 156
AluI AGCT 2 cut(s) 110, 156
Alw21I GWGCWC 1 cut(s) 158
Alw26I GTCTC 2 cut(s) 203, 417
AlwI GGATC 3 cut(s) 257, 270, 328
AlwNI CAGNNNCTG 1 cut(s) 113
Aor51HI AGCGCT 1 cut(s) 93
ApeKI GCWGC 2 cut(s) 94, 110
ApoI RAATTY 1 cut(s) 80
Asp700I GAANNNNTTC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 94
BamHI GGATCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 119
Bbv12I GWGCWC 1 cut(s) 158
BbvI GCAGC 2 cut(s) 81, 97
BciT130I CCWGG 1 cut(s) 445
BciVI GTATCC 1 cut(s) 220
BcoDI GTCTC 2 cut(s) 203, 417
BfmI CTRYAG 1 cut(s) 231
BfoI RGCGCY 1 cut(s) 95
BfrI CTTAAG 1 cut(s) 409
BfuI GTATCC 1 cut(s) 220
BisI GCNGC 2 cut(s) 95, 111
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 2 cut(s) 96, 112
BmcAI AGTACT 1 cut(s) 341
Bme1390I CCNGG 1 cut(s) 445
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 1 cut(s) 445
BmsI GCATC 1 cut(s) 342
BpiI GAAGAC 1 cut(s) 119
BpmI CTGGAG 1 cut(s) 427
Bpu1102I GCTNAGC 1 cut(s) 114
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bse1I ACTGG 2 cut(s) 246, 337
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 1 cut(s) 445
BseGI GGATG 1 cut(s) 301
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 105, 158
BseNI ACTGG 2 cut(s) 246, 337
BseXI GCAGC 2 cut(s) 81, 97
BsiHKAI GWGCWC 1 cut(s) 158
BslFI GGGAC 2 cut(s) 19, 32
BsmAI GTCTC 2 cut(s) 203, 417
BsmFI GGGAC 2 cut(s) 19, 32
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 3 cut(s) 262, 307, 333
Bsp1720I GCTNAGC 1 cut(s) 114
BspCNI CTCAG 2 cut(s) 106, 157
BspHI TCATGA 1 cut(s) 150
BspLI GGNNCC 1 cut(s) 264
BspPI GGATC 3 cut(s) 257, 270, 328
BspTI CTTAAG 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 2 cut(s) 246, 337
BssMI GATC 3 cut(s) 262, 307, 333
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 1 cut(s) 232
BstAFI CTTAAG 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 2 cut(s) 114, 144
BstF5I GGATG 1 cut(s) 301
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 3 cut(s) 265, 310, 336
BstMAI GTCTC 2 cut(s) 203, 417
BstMBI GATC 3 cut(s) 262, 307, 333
BstMWI GCNNNNNNNGC 1 cut(s) 426
BstNI CCWGG 1 cut(s) 445
BstSCI CCNGG 1 cut(s) 443
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 81, 97
BstV2I GAAGAC 1 cut(s) 119
BstX2I RGATCY 2 cut(s) 262, 333
BstYI RGATCY 2 cut(s) 262, 333
BsuI GTATCC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 60
CaiI CAGNNNCTG 1 cut(s) 113
CciI TCATGA 1 cut(s) 150
CfoI GCGC 1 cut(s) 94
Csp6I GTAC 2 cut(s) 340, 365
CviAII CATG 4 cut(s) 119, 151, 164, 469
CviJI RGCY 3 cut(s) 110, 136, 156
CviKI_1 RGCY 3 cut(s) 110, 136, 156
CviQI GTAC 2 cut(s) 340, 365
DdeI CTNAG 2 cut(s) 114, 144
DpnI GATC 3 cut(s) 264, 309, 335
DpnII GATC 3 cut(s) 262, 307, 333
DraI TTTAAA 1 cut(s) 402
DrdI GACNNNNNNGTC 1 cut(s) 431
DseDI GACNNNNNNGTC 1 cut(s) 431
Ecl136II GAGCTC 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 158
Eco47III AGCGCT 1 cut(s) 93
Eco53kI GAGCTC 1 cut(s) 156
EcoICRI GAGCTC 1 cut(s) 156
EcoRI GAATTC 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 443
EcoT38I GRGCYC 1 cut(s) 158
FaeI CATG 4 cut(s) 122, 154, 167, 472
FaqI GGGAC 2 cut(s) 19, 32
FatI CATG 4 cut(s) 118, 150, 163, 468
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 2 cut(s) 95, 111
FokI GGATG 1 cut(s) 308
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 2 cut(s) 95, 111
GlaI GCGC 1 cut(s) 93
GluI GCNGC 2 cut(s) 95, 111
GsuI CTGGAG 1 cut(s) 427
HaeII RGCGCY 1 cut(s) 95
HhaI GCGC 1 cut(s) 94
Hin1II CATG 4 cut(s) 122, 154, 167, 472
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
Hpy166II GTNNAC 2 cut(s) 367, 436
Hpy188III TCNNGA 6 cut(s) 77, 151, 200, 260, 305, 315
Hpy8I GTNNAC 2 cut(s) 367, 436
HpyAV CCTTC 2 cut(s) 43, 322
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 2 cut(s) 49, 124
HpyCH4V TGCA 2 cut(s) 163, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 426
HpyF3I CTNAG 2 cut(s) 114, 144
HpySE526I ACGT 2 cut(s) 49, 124
Hsp92II CATG 4 cut(s) 122, 154, 167, 472
HspAI GCGC 1 cut(s) 92
Kzo9I GATC 3 cut(s) 262, 307, 333
LmnI GCTCC 1 cut(s) 67
Lsp1109I GCAGC 2 cut(s) 81, 97
LweI GCATC 1 cut(s) 342
MaeII ACGT 2 cut(s) 49, 124
MalI GATC 3 cut(s) 264, 309, 335
MboI GATC 3 cut(s) 262, 307, 333
MboII GAAGA 2 cut(s) 119, 260
MflI RGATCY 2 cut(s) 262, 333
MhlI GDGCHC 1 cut(s) 158
MluCI AATT 2 cut(s) 80, 386
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 3 cut(s) 3, 50, 169
MroXI GAANNNNTTC 1 cut(s) 126
MseI TTAA 3 cut(s) 389, 401, 410
MslI CAYNNNNRTG 1 cut(s) 168
MspA1I CMGCKG 1 cut(s) 110
MspCI CTTAAG 1 cut(s) 409
MspR9I CCNGG 1 cut(s) 445
MvaI CCWGG 1 cut(s) 445
MwoI GCNNNNNNNGC 1 cut(s) 426
NdeII GATC 3 cut(s) 262, 307, 333
NlaIII CATG 4 cut(s) 122, 154, 167, 472
NlaIV GGNNCC 1 cut(s) 264
NmeAIII GCCGAG 1 cut(s) 123
PagI TCATGA 1 cut(s) 150
PdmI GAANNNNTTC 1 cut(s) 126
PkrI GCNGC 2 cut(s) 96, 112
Psp124BI GAGCTC 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 443
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 1 cut(s) 264
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 2 cut(s) 262, 333
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 2 cut(s) 341, 366
RsaNI GTAC 2 cut(s) 340, 365
RseI CAYNNNNRTG 1 cut(s) 168
SacI GAGCTC 1 cut(s) 158
SaqAI TTAA 3 cut(s) 389, 401, 410
SatI GCNGC 2 cut(s) 95, 111
Sau3AI GATC 3 cut(s) 262, 307, 333
ScaI AGTACT 1 cut(s) 341
ScrFI CCNGG 1 cut(s) 445
SduI GDGCHC 1 cut(s) 158
SetI ASST 9 cut(s) 42, 52, 112, 127, 150, 158, 213, 366, 449
SfaNI GCATC 1 cut(s) 342
SfcI CTRYAG 1 cut(s) 231
SmiMI CAYNNNNRTG 1 cut(s) 168
SmlI CTYRAG 1 cut(s) 409
SmoI CTYRAG 1 cut(s) 409
Sse9I AATT 2 cut(s) 80, 386
SstI GAGCTC 1 cut(s) 158
StyD4I CCNGG 1 cut(s) 443
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 2 cut(s) 52, 127
TaqI TCGA 1 cut(s) 201
TasI AATT 2 cut(s) 80, 386
TatI WGTACW 1 cut(s) 339
Tru1I TTAA 3 cut(s) 389, 401, 410
Tru9I TTAA 3 cut(s) 389, 401, 410
TseI GCWGC 2 cut(s) 94, 110
TspDTI ATGAA 2 cut(s) 135, 385
Vha464I CTTAAG 1 cut(s) 409
XapI RAATTY 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 435
XmnI GAANNNNTTC 1 cut(s) 126
ZrmI AGTACT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.