FvH4_5g25140

Nnf1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010244
Physical Location & Seq
Forward (+)
16608015 .. 16612130
4116 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25140.t1

Sequence Viewer

Length: 516 bp
ATGGGGACGAAGCAAAGGGACTTGCACAAGTCCTTCAACCTCGCCATACGTTCTCTCCTCTCTCCTTGCTCCTTCCAGGACTTCTGGGAAGCTCTTCCCAATTTCACGACTGCTGAGCATGAACGTCTTCACAGGCTCTTCACTCAGGTCCTGAGTTCTTTACATGCAAATATAGAGGAAGAGTTTCAGTCTATATGTCTTGAAACACAGGTTGGAACTGCAATGGATACTGTAGAAAGAATGGTTGAAGAACAAAGTCTGGATCCTTTATATTCAAATAAGACTAATGTAATGGATGCTGCTCACAATCTTTCGGGAACAACAAAGACGGAGATCCAGTATTTGACAAAGATGCTTGAAAAGGCGGAGGCAGAGAATCACCTTAAGCGGGATCGTATTGAATTGATAAAGAAAGGAAGGCCGGATATGTCGAACATGGCAAATGCTGTTGAGAAGATGAGAAGTGGGATTTCAAGTTATGGAATGTCTCGCAGCAATGGGGTCCATAGTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nnf1

Protein Analysis

172

Amino Acids

19.45

Weight (kDa)

6.16

Isoelectric Point (pI)

56.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nnf1 PF03980 43 - 134 1.6e-07 Nnf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 365, 388
AclWI GGATC 4 cut(s) 257, 270, 328, 399
AfiI CCNNNNNNNGG 1 cut(s) 388
AflII CTTAAG 1 cut(s) 383
AgsI TTSAA 7 cut(s) 37, 203, 248, 276, 359, 401, 474
AjnI CCWGG 1 cut(s) 75
AjuI GAANNNNNNNTTGG 2 cut(s) 195, 227
AluBI AGCT 1 cut(s) 92
AluI AGCT 1 cut(s) 92
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 4 cut(s) 257, 270, 328, 399
AlwNI CAGNNNCTG 1 cut(s) 151
AoxI GGCC 1 cut(s) 419
ApeKI GCWGC 2 cut(s) 299, 492
Asp700I GAANNNNTTC 3 cut(s) 93, 126, 183
AspS9I GGNCC 2 cut(s) 148, 502
AsuHPI GGTGA 1 cut(s) 371
AvaII GGWCC 2 cut(s) 148, 502
BamHI GGATCC 1 cut(s) 262
BbsI GAAGAC 1 cut(s) 119
BbvI GCAGC 2 cut(s) 286, 504
BciT130I CCWGG 1 cut(s) 77
BciVI GTATCC 1 cut(s) 220
BcoDI GTCTC 1 cut(s) 492
BfmI CTRYAG 1 cut(s) 231
BfrI CTTAAG 1 cut(s) 383
BfuI GTATCC 1 cut(s) 220
BisI GCNGC 2 cut(s) 300, 493
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 2 cut(s) 301, 494
Bme1390I CCNGG 1 cut(s) 77
Bme18I GGWCC 2 cut(s) 148, 502
BmgT120I GGNCC 2 cut(s) 148, 502
BmiI GGNNCC 2 cut(s) 264, 503
BmrFI CCNGG 1 cut(s) 77
BmsI GCATC 2 cut(s) 286, 342
BpiI GAAGAC 1 cut(s) 119
Bpu1102I GCTNAGC 1 cut(s) 114
BsaXI ACNNNNNCTCC 4 cut(s) 39, 69, 323, 353
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse1I ACTGG 1 cut(s) 337
Bse3DI GCAATG 2 cut(s) 228, 502
BseBI CCWGG 1 cut(s) 77
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMI GCAATG 2 cut(s) 228, 502
BseMII CTCAG 3 cut(s) 105, 143, 158
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 2 cut(s) 286, 504
BshFI GGCC 1 cut(s) 421
BsiSI CCGG 1 cut(s) 422
BslFI GGGAC 2 cut(s) 19, 32
BslI CCNNNNNNNGG 1 cut(s) 388
BsmAI GTCTC 1 cut(s) 492
BsmFI GGGAC 2 cut(s) 19, 32
BsnI GGCC 1 cut(s) 421
Bsp143I GATC 3 cut(s) 262, 333, 391
Bsp1720I GCTNAGC 1 cut(s) 114
BspACI CCGC 2 cut(s) 365, 388
BspANI GGCC 1 cut(s) 421
BspCNI CTCAG 3 cut(s) 106, 144, 157
BspLI GGNNCC 2 cut(s) 264, 503
BspPI GGATC 4 cut(s) 257, 270, 328, 399
BspQI GCTCTTC 2 cut(s) 99, 143
BspTI CTTAAG 1 cut(s) 383
BsrDI GCAATG 2 cut(s) 228, 502
BsrI ACTGG 1 cut(s) 337
BssMI GATC 3 cut(s) 262, 333, 391
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 3 cut(s) 99, 143, 174
BstAFI CTTAAG 1 cut(s) 383
BstDEI CTNAG 3 cut(s) 114, 144, 152
BstF5I GGATG 1 cut(s) 301
BstKTI GATC 3 cut(s) 265, 336, 394
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 3 cut(s) 262, 333, 391
BstNI CCWGG 1 cut(s) 77
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 75
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 286, 504
BstV2I GAAGAC 1 cut(s) 119
BstX2I RGATCY 2 cut(s) 262, 333
BstYI RGATCY 2 cut(s) 262, 333
BsuI GTATCC 1 cut(s) 220
BsuRI GGCC 1 cut(s) 421
BtsCI GGATG 1 cut(s) 301
CaiI CAGNNNCTG 1 cut(s) 151
Cfr13I GGNCC 2 cut(s) 148, 502
CviAII CATG 3 cut(s) 119, 164, 436
CviJI RGCY 3 cut(s) 92, 136, 421
CviKI_1 RGCY 3 cut(s) 92, 136, 421
DdeI CTNAG 3 cut(s) 114, 144, 152
DpnI GATC 3 cut(s) 264, 335, 393
DpnII GATC 3 cut(s) 262, 333, 391
Eam1104I CTCTTC 3 cut(s) 99, 143, 174
EarI CTCTTC 3 cut(s) 99, 143, 174
EciI GGCGGA 1 cut(s) 380
Eco47I GGWCC 2 cut(s) 148, 502
EcoO109I RGGNCCY 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 75
FaeI CATG 3 cut(s) 122, 167, 439
FaqI GGGAC 2 cut(s) 19, 32
FatI CATG 3 cut(s) 118, 163, 435
FauI CCCGC 1 cut(s) 381
Fnu4HI GCNGC 2 cut(s) 300, 493
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 2 cut(s) 300, 493
GluI GCNGC 2 cut(s) 300, 493
HaeIII GGCC 1 cut(s) 421
HapII CCGG 1 cut(s) 422
Hin1II CATG 3 cut(s) 122, 167, 439
HinfI GANTC 1 cut(s) 376
HpaII CCGG 1 cut(s) 422
HphI GGTGA 1 cut(s) 371
Hpy188III TCNNGA 5 cut(s) 106, 151, 200, 260, 315
HpyAV CCTTC 3 cut(s) 43, 82, 411
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 2 cut(s) 49, 124
HpyCH4V TGCA 3 cut(s) 25, 167, 221
HpyF3I CTNAG 3 cut(s) 114, 144, 152
HpySE526I ACGT 2 cut(s) 49, 124
Hsp92II CATG 3 cut(s) 122, 167, 439
Kzo9I GATC 3 cut(s) 262, 333, 391
LguI GCTCTTC 2 cut(s) 99, 143
LmnI GCTCC 1 cut(s) 74
Lsp1109I GCAGC 2 cut(s) 286, 504
LweI GCATC 2 cut(s) 286, 342
MaeII ACGT 2 cut(s) 49, 124
MalI GATC 3 cut(s) 264, 335, 393
MboI GATC 3 cut(s) 262, 333, 391
MboII GAAGA 6 cut(s) 86, 119, 130, 191, 260, 466
MflI RGATCY 2 cut(s) 262, 333
MluCI AATT 2 cut(s) 100, 401
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 4 cut(s) 50, 68, 169, 361
MroXI GAANNNNTTC 3 cut(s) 93, 126, 183
MseI TTAA 1 cut(s) 384
MspCI CTTAAG 1 cut(s) 383
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
NdeII GATC 3 cut(s) 262, 333, 391
NlaIII CATG 3 cut(s) 122, 167, 439
NlaIV GGNNCC 2 cut(s) 264, 503
NspI RCATGY 1 cut(s) 167
PciSI GCTCTTC 2 cut(s) 99, 143
PdmI GAANNNNTTC 3 cut(s) 93, 126, 183
PfeI GAWTC 1 cut(s) 376
PfoI TCCNGGA 1 cut(s) 75
PkrI GCNGC 2 cut(s) 301, 494
PpuMI RGGWCCY 1 cut(s) 148
Psp5II RGGWCCY 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 2 cut(s) 264, 503
PspPI GGNCC 2 cut(s) 148, 502
PspPPI RGGWCCY 1 cut(s) 148
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 2 cut(s) 262, 333
SapI GCTCTTC 2 cut(s) 99, 143
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 300, 493
Sau3AI GATC 3 cut(s) 262, 333, 391
Sau96I GGNCC 2 cut(s) 148, 502
ScrFI CCNGG 1 cut(s) 77
SetI ASST 7 cut(s) 42, 52, 94, 127, 150, 213, 384
SfaNI GCATC 2 cut(s) 286, 342
SfcI CTRYAG 1 cut(s) 231
SinI GGWCC 2 cut(s) 148, 502
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 2 cut(s) 100, 401
SsiI CCGC 2 cut(s) 365, 388
StyD4I CCNGG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 2 cut(s) 52, 127
TaqI TCGA 1 cut(s) 431
TasI AATT 2 cut(s) 100, 401
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseI GCWGC 2 cut(s) 299, 492
TspDTI ATGAA 1 cut(s) 135
TspGWI ACGGA 2 cut(s) 344, 501
Vha464I CTTAAG 1 cut(s) 383
VpaK11BI GGWCC 2 cut(s) 148, 502
XceI RCATGY 1 cut(s) 167
XmnI GAANNNNTTC 3 cut(s) 93, 126, 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.