MD03G1130300.v1.1

Nnf1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010244
Physical Location & Seq
Forward (+)
12815434 .. 12820150
4717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1130300.v1.1.491

Sequence Viewer

Length: 495 bp
ATGGGGACGAAGGAATTGGACATGAAGAAAGCCTTCAAGTTGGCCATACGTTCTCTGCTCACTCCCTGTTCCAAGCAGGAATTCTGCCAAGCATTTCCGAATTTTACATTTGCAGAGCAAGAACACCTTCATAAGCTTTTTATCCAGGTCATCGCTTCTTTGCATGGAAATTTAGAGGACGAGTTTCAGTCAGCATGCCTTGAAACACAGGTGGGAACTGTTCTGGACACCGTTGAACAACTTGTGGAAGAACAAGCTATGGATCCTTTGTCTTCAGATAAGACTAATCTAATGGATGTTGCTCGCGATCTGTCCACAATAAAGAAGGATGAAATCCAGAACTTGACGCAAAGGCTTGGAATGGCTAAGGAACAGAACCACCATCTTCGGGATCGTATACAACTTTTAAAGAAAGAAGGGGTAGATTTGTCAGGCTTGGAGAATGCTGTTGAGAAGCTGAGAAGTGGGAGTTTAATGTACGGAAATGTCGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nnf1

Protein Analysis

165

Amino Acids

18.49

Weight (kDa)

5.38

Isoelectric Point (pI)

28.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nnf1 PF03980 42 - 137 9.7e-07 Nnf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 397
AccII CGCG 1 cut(s) 306
AclWI GGATC 3 cut(s) 257, 270, 399
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 3 cut(s) 80, 100, 169
AcuI CTGAAG 1 cut(s) 258
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 1 cut(s) 388
AgsI TTSAA 3 cut(s) 37, 203, 236
AjnI CCWGG 1 cut(s) 144
AjuI GAANNNNNNNTTGG 2 cut(s) 65, 97
AluBI AGCT 3 cut(s) 136, 257, 457
AluI AGCT 3 cut(s) 136, 257, 457
AlwI GGATC 3 cut(s) 257, 270, 399
AoxI GGCC 1 cut(s) 42
ApoI RAATTY 3 cut(s) 80, 100, 169
Asp700I GAANNNNTTC 2 cut(s) 32, 126
BalI TGGCCA 1 cut(s) 44
BamHI GGATCC 1 cut(s) 262
BbsI GAAGAC 1 cut(s) 264
BccI CCATC 1 cut(s) 390
BciT130I CCWGG 1 cut(s) 146
Bme1390I CCNGG 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 366
Bsc4I CCNNNNNNNGG 1 cut(s) 388
BseBI CCWGG 1 cut(s) 146
BseGI GGATG 2 cut(s) 301, 334
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMII CTCAG 1 cut(s) 449
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 1 cut(s) 44
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 448
BsnI GGCC 1 cut(s) 44
Bsp143I GATC 3 cut(s) 262, 307, 391
Bsp68I TCGCGA 1 cut(s) 306
BspANI GGCC 1 cut(s) 44
BspCNI CTCAG 1 cut(s) 450
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 1 cut(s) 264
BspPI GGATC 3 cut(s) 257, 270, 399
BssMI GATC 3 cut(s) 262, 307, 391
BssNAI GTATAC 1 cut(s) 398
Bst1107I GTATAC 1 cut(s) 398
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 220, 232
BstC8I GCNNGC 2 cut(s) 196, 304
BstDEI CTNAG 2 cut(s) 366, 458
BstF5I GGATG 2 cut(s) 301, 334
BstFNI CGCG 1 cut(s) 306
BstKTI GATC 3 cut(s) 265, 310, 394
BstMBI GATC 3 cut(s) 262, 307, 391
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 144
BstUI CGCG 1 cut(s) 306
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BstZ17I GTATAC 1 cut(s) 398
BsuRI GGCC 1 cut(s) 44
BtgZI GCGATG 1 cut(s) 136
BtsCI GGATG 2 cut(s) 301, 334
BtuMI TCGCGA 1 cut(s) 306
Cac8I GCNNGC 2 cut(s) 196, 304
CseI GACGC 1 cut(s) 355
Csp6I GTAC 1 cut(s) 478
CviAII CATG 3 cut(s) 22, 164, 195
CviJI RGCY 8 cut(s) 32, 44, 136, 257, 355, 365, 435, 457
CviKI_1 RGCY 8 cut(s) 32, 44, 136, 257, 355, 365, 435, 457
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 2 cut(s) 366, 458
DpnI GATC 3 cut(s) 264, 309, 393
DpnII GATC 3 cut(s) 262, 307, 391
DraI TTTAAA 1 cut(s) 408
EaeI YGGCCR 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 258
EcoRI GAATTC 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 144
FaeI CATG 3 cut(s) 25, 167, 198
FaiI YATR 7 cut(s) 23, 47, 132, 165, 196, 260, 398
FalI AAGNNNNNCTT 4 cut(s) 17, 49, 111, 143
FaqI GGGAC 1 cut(s) 19
FatI CATG 3 cut(s) 21, 163, 194
FblI GTMKAC 1 cut(s) 397
FokI GGATG 2 cut(s) 308, 341
HaeIII GGCC 1 cut(s) 44
HgaI GACGC 1 cut(s) 355
Hin1II CATG 3 cut(s) 25, 167, 198
HindIII AAGCTT 1 cut(s) 134
Hpy166II GTNNAC 2 cut(s) 315, 398
Hpy188I TCNGA 3 cut(s) 99, 277, 491
Hpy188III TCNNGA 4 cut(s) 224, 305, 337, 389
Hpy8I GTNNAC 2 cut(s) 315, 398
HpyAV CCTTC 5 cut(s) 4, 43, 137, 319, 410
HpyCH4III ACNGT 2 cut(s) 220, 232
HpyCH4IV ACGT 1 cut(s) 49
HpyCH4V TGCA 2 cut(s) 113, 163
HpyF3I CTNAG 2 cut(s) 366, 458
HpySE526I ACGT 1 cut(s) 49
Hsp92II CATG 3 cut(s) 25, 167, 198
Kzo9I GATC 3 cut(s) 262, 307, 391
LpnPI CCDG 8 cut(s) 62, 79, 131, 158, 194, 209, 350, 417
MaeII ACGT 1 cut(s) 49
MalI GATC 3 cut(s) 264, 309, 393
MboI GATC 3 cut(s) 262, 307, 391
MboII GAAGA 4 cut(s) 37, 260, 264, 377
MflI RGATCY 1 cut(s) 262
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 4 cut(s) 14, 80, 100, 169
MluNI TGGCCA 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 469
MnlI CCTC 1 cut(s) 169
Mox20I TGGCCA 1 cut(s) 44
MroXI GAANNNNTTC 2 cut(s) 32, 126
MscI TGGCCA 1 cut(s) 44
MseI TTAA 2 cut(s) 407, 473
Msp20I TGGCCA 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 146
Mva1269I GAATGC 1 cut(s) 448
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 306
NdeII GATC 3 cut(s) 262, 307, 391
NlaIII CATG 3 cut(s) 25, 167, 198
NlaIV GGNNCC 1 cut(s) 264
NruI TCGCGA 1 cut(s) 306
NspI RCATGY 1 cut(s) 198
PaeI GCATGC 1 cut(s) 198
PctI GAATGC 1 cut(s) 448
PdmI GAANNNNTTC 2 cut(s) 32, 126
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 264
PsuI RGATCY 1 cut(s) 262
RruI TCGCGA 1 cut(s) 306
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
SaqAI TTAA 2 cut(s) 407, 473
Sau3AI GATC 3 cut(s) 262, 307, 391
ScrFI CCNGG 1 cut(s) 146
SetI ASST 7 cut(s) 52, 129, 138, 150, 213, 259, 459
SphI GCATGC 1 cut(s) 198
Sse9I AATT 4 cut(s) 14, 80, 100, 169
StyD4I CCNGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 220, 232
TaiI ACGT 1 cut(s) 52
TasI AATT 4 cut(s) 14, 80, 100, 169
Tru1I TTAA 2 cut(s) 407, 473
Tru9I TTAA 2 cut(s) 407, 473
TspDTI ATGAA 3 cut(s) 38, 119, 345
TspGWI ACGGA 1 cut(s) 495
XapI RAATTY 3 cut(s) 80, 100, 169
XceI RCATGY 1 cut(s) 198
XmiI GTMKAC 1 cut(s) 397
XmnI GAANNNNTTC 2 cut(s) 32, 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.