Rorug07G0152200

Nnf1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010244
Physical Location & Seq
Reverse (-)
12075591 .. 12077221
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0152200.1

Sequence Viewer

Length: 1380 bp
ATGTGTGCTAGATTTTGGTGGGGAAGTACTCTTGATAAGAGGAAAATTCATTGGAAGACTTGGCAAGCATTGTGTAATCCAAAGGAAGCTGGGGGGCTTGGTTTTAGAAGTCTTTCTAATTTTAATGAAGCAATGTTGGCTAAACAAGTATGGCGGGTGATTACTAATCCTTCCTCGCTGGTGGCTCGTCTGTATAAGGCGAAGTATTTCCCGGATGGCTCTTTTTGGGATGCTCCAGTTCATGATTCTCCTTCCTACTCATGGAGAAGGAAACCTCTACGGACGGAGTTGGCTCAAAGTGAGGTCACTATGGTACAAGATCTGATCTTAGACACGGGGTTGTGGAATGTGGCAAAGATTGGGAGGTTGTTTCCAGTATTGGAGGCGGAGAGAATTATTAGTATACCGTTAAGTCGACGGGTGGGGGCAGATAGGGTGGTTTGGAGGTTAACAAAGGATGGTAAGTTTTCAGTTAACTCTGCTTATCGTCGTTCTTTTTCTAATTCTTTGAGCTCCACTTCTTTTCTTACTTCGATGGGATCTGCTCTCTGGAAGAAGGTGTGGAAGGTTGCTATTCCAAATAAAGCTAAGGTTCATGTTTGGAGGGTTTGTTTGGATATGTTGCCATCTCTTGGTAAGTTGGAGTCGAGAAGAGTGCAGCTGGACTCTGTTATGGATTGTCCTTTTACTCAAGAAGTTTTGAAGTCCAATGTTGTTCTTAGGCAAGTGTGCTACAGTCCTCAGTTGGATGGTAGTGATTTGTTCCAGTGGATGAGTTCTTGTGCGAATGATTTGTCACCGATCAGGTTTGGGGATTTGCTCTTTCTTTTGTTCAGTGTGTGGAAGGAGAGGAATGACCGAGTTTGGCACCAGAAGAAACAGGGGACGGGTGATGTGGCTCCACCGGTGGGGATTTTGAAGATTAATGTTGACGGGGCCTTTAATCATGTTACCCGTAAAGGTGGTGTGGGTTTTGTTATCCGAAATGAGTTAGGCGTTATGCTGGCAGGAGGTGCTTGCCCTCTAAGTGGTTTGTTATCGGCGGAACACAGGGAGGTCTTAGCTTGCCAGAAAGCACTTGAATTTGCGGTGGCTCATGCCTTTTTACCTGCGATTCTGGAAACAGACGCATTGGAAGTCCAACGCCAATTGAATGCAGTTGTGGAGATTAATACTTCCGTGCTGGGAAGGGTTTATGAAGATTTGGTGCTCCTAATGGCATCTCAACCTTCTGTGTCTGTCGCTCATGTTGGCAGGTTGGGCAACACTGTTGCTCATAGTTTAGCAGCTTATGCTTGTAGTTTGCAGCAGGACTGCTTTTATTTCTCTGCTCCGTCTTTTCTCTTAGCTGCAGTAGCAGCTGAACTTTGCATTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nnf1

Protein Analysis

459

Amino Acids

51.18

Weight (kDa)

9.32

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 309 - 432 7e-20 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1117, 1245
AccB1I GGYRCC 1 cut(s) 867
AccB7I CCANNNNNTGG 1 cut(s) 632
AccI GTMKAC 2 cut(s) 403, 415
AciI CCGC 4 cut(s) 154, 386, 1043, 1088
AclWI GGATC 1 cut(s) 547
AcsI RAATTY 2 cut(s) 45, 1082
AfaI GTAC 2 cut(s) 28, 315
AfiI CCNNNNNNNGG 4 cut(s) 261, 632, 908, 1028
AgeI ACCGGT 1 cut(s) 904
AgsI TTSAA 4 cut(s) 703, 919, 1082, 1153
AluBI AGCT 8 cut(s) 89, 513, 587, 661, 1064, 1289, 1349, 1361
AluI AGCT 8 cut(s) 89, 513, 587, 661, 1064, 1289, 1349, 1361
Alw21I GWGCWC 2 cut(s) 515, 1212
AlwI GGATC 1 cut(s) 547
AoxI GGCC 1 cut(s) 936
ApeKI GCWGC 5 cut(s) 658, 1286, 1306, 1349, 1358
ApoI RAATTY 2 cut(s) 45, 1082
ArsI GACNNNNNNTTYG 2 cut(s) 779, 811
AseI ATTAAT 2 cut(s) 924, 1170
AsiGI ACCGGT 1 cut(s) 904
Asp700I GAANNNNTTC 2 cut(s) 112, 206
AspS9I GGNCC 1 cut(s) 936
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 3 cut(s) 169, 789, 902
BanI GGYRCC 1 cut(s) 867
BanII GRGCYC 1 cut(s) 515
BbsI GAAGAC 1 cut(s) 62
Bbv12I GWGCWC 2 cut(s) 515, 1212
BbvI GCAGC 5 cut(s) 670, 1298, 1318, 1336, 1370
BccI CCATC 5 cut(s) 209, 452, 529, 634, 743
BcgI CGANNNNNNTGC 2 cut(s) 637, 671
BcnI CCSGG 1 cut(s) 212
BfaI CTAG 1 cut(s) 9
BfmI CTRYAG 2 cut(s) 733, 1350
BfuAI ACCTGC 2 cut(s) 1117, 1245
BglII AGATCT 1 cut(s) 319
BisI GCNGC 5 cut(s) 659, 1287, 1307, 1350, 1359
BlsI GCNGC 5 cut(s) 660, 1288, 1308, 1351, 1360
BmcAI AGTACT 1 cut(s) 28
Bme1390I CCNGG 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 936
BmiI GGNNCC 3 cut(s) 869, 900, 937
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 2 cut(s) 220, 1229
BpiI GAAGAC 1 cut(s) 62
BpmI CTGGAG 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 675
BpuMI CCSGG 1 cut(s) 212
BsaWI WCCGGW 1 cut(s) 904
BsaXI ACNNNNNCTCC 2 cut(s) 595, 625
Bsc4I CCNNNNNNNGG 4 cut(s) 261, 632, 908, 1028
Bse118I RCCGGY 1 cut(s) 904
Bse1I ACTGG 3 cut(s) 236, 374, 766
Bse3DI GCAATG 1 cut(s) 138
BseGI GGATG 5 cut(s) 220, 235, 463, 754, 777
BseLI CCNNNNNNNGG 4 cut(s) 261, 632, 908, 1028
BseMI GCAATG 1 cut(s) 138
BseMII CTCAG 1 cut(s) 755
BseNI ACTGG 3 cut(s) 236, 374, 766
BseXI GCAGC 5 cut(s) 670, 1298, 1318, 1336, 1370
BseYI CCCAGC 2 cut(s) 89, 1183
BsgI GTGCAG 1 cut(s) 677
BshFI GGCC 1 cut(s) 938
BshNI GGYRCC 1 cut(s) 867
BshTI ACCGGT 1 cut(s) 904
BsiHKAI GWGCWC 2 cut(s) 515, 1212
BsiSI CCGG 2 cut(s) 212, 905
BslFI GGGAC 1 cut(s) 898
BslI CCNNNNNNNGG 4 cut(s) 261, 632, 908, 1028
BsmFI GGGAC 1 cut(s) 898
BsmI GAATGC 1 cut(s) 1159
BsnI GGCC 1 cut(s) 938
Bsp1286I GDGCHC 2 cut(s) 515, 1212
Bsp143I GATC 4 cut(s) 319, 324, 539, 801
BspACI CCGC 4 cut(s) 154, 386, 1043, 1088
BspANI GGCC 1 cut(s) 938
BspCNI CTCAG 1 cut(s) 754
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 3 cut(s) 869, 900, 937
BspMAI CTGCAG 1 cut(s) 1354
BspMI ACCTGC 2 cut(s) 1117, 1245
BspPI GGATC 1 cut(s) 547
BspT107I GGYRCC 1 cut(s) 867
BsrDI GCAATG 1 cut(s) 138
BsrFI RCCGGY 1 cut(s) 904
BsrI ACTGG 3 cut(s) 236, 374, 766
BssAI RCCGGY 1 cut(s) 904
BssMI GATC 4 cut(s) 319, 324, 539, 801
BssNAI GTATAC 1 cut(s) 404
Bst1107I GTATAC 1 cut(s) 404
Bst4CI ACNGT 3 cut(s) 408, 737, 1270
Bst6I CTCTTC 1 cut(s) 646
BstAPI GCANNNNNTGC 2 cut(s) 1013, 1292
BstC8I GCNNGC 4 cut(s) 66, 1005, 1018, 1066
BstDEI CTNAG 7 cut(s) 328, 588, 719, 741, 1025, 1060, 1345
BstF5I GGATG 5 cut(s) 220, 235, 463, 754, 777
BstKTI GATC 4 cut(s) 322, 327, 542, 804
BstMBI GATC 4 cut(s) 319, 324, 539, 801
BstMWI GCNNNNNNNGC 6 cut(s) 137, 1013, 1260, 1292, 1355, 1358
BstSCI CCNGG 1 cut(s) 210
BstSFI CTRYAG 2 cut(s) 733, 1350
BstV1I GCAGC 5 cut(s) 670, 1298, 1318, 1336, 1370
BstV2I GAAGAC 1 cut(s) 62
BstX2I RGATCY 2 cut(s) 319, 539
BstYI RGATCY 2 cut(s) 319, 539
BstZ17I GTATAC 1 cut(s) 404
BsuRI GGCC 1 cut(s) 938
BtsCI GGATG 5 cut(s) 220, 235, 463, 754, 777
BtsIMutI CAGTG 3 cut(s) 773, 841, 1266
BveI ACCTGC 2 cut(s) 1117, 1245
Cac8I GCNNGC 4 cut(s) 66, 1005, 1018, 1066
CciI TCATGA 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 904
Cfr13I GGNCC 1 cut(s) 936
CseI GACGC 1 cut(s) 1136
Csp6I GTAC 2 cut(s) 27, 314
CspAI ACCGGT 1 cut(s) 904
CviAII CATG 6 cut(s) 242, 261, 596, 947, 1097, 1247
CviQI GTAC 2 cut(s) 27, 314
DdeI CTNAG 7 cut(s) 328, 588, 719, 741, 1025, 1060, 1345
DpnI GATC 4 cut(s) 321, 326, 541, 803
DpnII GATC 4 cut(s) 319, 324, 539, 801
Eam1104I CTCTTC 1 cut(s) 646
EarI CTCTTC 1 cut(s) 646
EciI GGCGGA 2 cut(s) 401, 1058
Ecl136II GAGCTC 1 cut(s) 513
Eco24I GRGCYC 1 cut(s) 515
Eco53kI GAGCTC 1 cut(s) 513
EcoICRI GAGCTC 1 cut(s) 513
EcoO109I RGGNCCY 1 cut(s) 936
EcoT38I GRGCYC 1 cut(s) 515
FaeI CATG 6 cut(s) 245, 264, 599, 950, 1100, 1250
FaqI GGGAC 1 cut(s) 898
FatI CATG 6 cut(s) 241, 260, 595, 946, 1096, 1246
FauI CCCGC 1 cut(s) 147
FblI GTMKAC 2 cut(s) 403, 415
Fnu4HI GCNGC 5 cut(s) 659, 1287, 1307, 1350, 1359
FokI GGATG 5 cut(s) 227, 242, 470, 761, 784
FriOI GRGCYC 1 cut(s) 515
Fsp4HI GCNGC 5 cut(s) 659, 1287, 1307, 1350, 1359
FspBI CTAG 1 cut(s) 9
GluI GCNGC 5 cut(s) 659, 1287, 1307, 1350, 1359
GsaI CCCAGC 2 cut(s) 93, 1187
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 1 cut(s) 938
HapII CCGG 2 cut(s) 212, 905
HgaI GACGC 1 cut(s) 1136
Hin1II CATG 6 cut(s) 245, 264, 599, 950, 1100, 1250
HincII GTYRAC 4 cut(s) 416, 450, 475, 931
HindII GTYRAC 4 cut(s) 416, 450, 475, 931
HinfI GANTC 4 cut(s) 245, 644, 665, 1114
HpaI GTTAAC 2 cut(s) 450, 475
HpaII CCGG 2 cut(s) 212, 905
HphI GGTGA 3 cut(s) 169, 789, 902
Hpy166II GTNNAC 5 cut(s) 404, 416, 450, 475, 931
Hpy188I TCNGA 2 cut(s) 324, 983
Hpy188III TCNNGA 6 cut(s) 32, 242, 550, 648, 692, 1118
Hpy8I GTNNAC 5 cut(s) 404, 416, 450, 475, 931
Hpy99I CGWCG 2 cut(s) 420, 492
HpyAV CCTTC 8 cut(s) 180, 261, 261, 550, 559, 838, 1182, 1239
HpyCH4III ACNGT 3 cut(s) 408, 737, 1270
HpyCH4V TGCA 5 cut(s) 658, 1157, 1306, 1352, 1371
HpyF10VI GCNNNNNNNGC 6 cut(s) 137, 1013, 1260, 1292, 1355, 1358
HpyF3I CTNAG 7 cut(s) 328, 588, 719, 741, 1025, 1060, 1345
Hsp92II CATG 6 cut(s) 245, 264, 599, 950, 1100, 1250
KspAI GTTAAC 2 cut(s) 450, 475
Kzo9I GATC 4 cut(s) 319, 324, 539, 801
LmnI GCTCC 5 cut(s) 238, 518, 904, 1215, 1336
Lsp1109I GCAGC 5 cut(s) 670, 1298, 1318, 1336, 1370
LweI GCATC 2 cut(s) 220, 1229
MaeI CTAG 1 cut(s) 9
MaeIII GTNAC 3 cut(s) 304, 795, 949
MalI GATC 4 cut(s) 321, 326, 541, 803
MboI GATC 4 cut(s) 319, 324, 539, 801
MboII GAAGA 6 cut(s) 67, 565, 663, 886, 931, 1211
MfeI CAATTG 1 cut(s) 1148
MflI RGATCY 2 cut(s) 319, 539
MhlI GDGCHC 2 cut(s) 515, 1212
MluCI AATT 6 cut(s) 45, 118, 393, 502, 1082, 1148
MlyI GAGTC 2 cut(s) 653, 659
MmeI TCCRAC 3 cut(s) 621, 726, 1165
MroXI GAANNNNTTC 2 cut(s) 112, 206
MseI TTAA 7 cut(s) 123, 410, 449, 474, 924, 942, 1170
MspA1I CMGCKG 2 cut(s) 661, 1361
MspI CCGG 2 cut(s) 212, 905
MspR9I CCNGG 1 cut(s) 212
MunI CAATTG 1 cut(s) 1148
Mva1269I GAATGC 1 cut(s) 1159
MwoI GCNNNNNNNGC 6 cut(s) 137, 1013, 1260, 1292, 1355, 1358
NciI CCSGG 1 cut(s) 212
NdeII GATC 4 cut(s) 319, 324, 539, 801
NlaIII CATG 6 cut(s) 245, 264, 599, 950, 1100, 1250
NlaIV GGNNCC 3 cut(s) 869, 900, 937
NmuCI GTSAC 2 cut(s) 304, 795
PagI TCATGA 1 cut(s) 241
PctI GAATGC 1 cut(s) 1159
PdmI GAANNNNTTC 2 cut(s) 112, 206
PfeI GAWTC 2 cut(s) 245, 1114
PflMI CCANNNNNTGG 1 cut(s) 632
PfoI TCCNGGA 1 cut(s) 210
PinAI ACCGGT 1 cut(s) 904
PkrI GCNGC 5 cut(s) 660, 1288, 1308, 1351, 1360
PleI GAGTC 2 cut(s) 652, 659
PpsI GAGTC 2 cut(s) 652, 659
PshBI ATTAAT 2 cut(s) 924, 1170
Psp124BI GAGCTC 1 cut(s) 515
PspFI CCCAGC 2 cut(s) 89, 1183
PspN4I GGNNCC 3 cut(s) 869, 900, 937
PspPI GGNCC 1 cut(s) 936
PstI CTGCAG 1 cut(s) 1354
PsuI RGATCY 2 cut(s) 319, 539
PvuII CAGCTG 2 cut(s) 661, 1361
RsaI GTAC 2 cut(s) 28, 315
RsaNI GTAC 2 cut(s) 27, 314
SacI GAGCTC 1 cut(s) 515
SalI GTCGAC 1 cut(s) 414
SaqAI TTAA 7 cut(s) 123, 410, 449, 474, 924, 942, 1170
SatI GCNGC 5 cut(s) 659, 1287, 1307, 1350, 1359
Sau3AI GATC 4 cut(s) 319, 324, 539, 801
Sau96I GGNCC 1 cut(s) 936
ScaI AGTACT 1 cut(s) 28
SchI GAGTC 2 cut(s) 653, 659
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 2 cut(s) 515, 1212
SfaNI GCATC 2 cut(s) 220, 1229
SfcI CTRYAG 2 cut(s) 733, 1350
SgrAI CRCCGGYG 1 cut(s) 904
SmlI CTYRAG 1 cut(s) 690
SmoI CTYRAG 1 cut(s) 690
Sse9I AATT 6 cut(s) 45, 118, 393, 502, 1082, 1148
SsiI CCGC 4 cut(s) 154, 386, 1043, 1088
SspMI CTAG 1 cut(s) 9
SstI GAGCTC 1 cut(s) 515
StyD4I CCNGG 1 cut(s) 210
TaaI ACNGT 3 cut(s) 408, 737, 1270
TaqI TCGA 3 cut(s) 415, 533, 647
TaqII GACCGA 1 cut(s) 873
TasI AATT 6 cut(s) 45, 118, 393, 502, 1082, 1148
TatI WGTACW 1 cut(s) 26
TfiI GAWTC 2 cut(s) 245, 1114
Tru1I TTAA 7 cut(s) 123, 410, 449, 474, 924, 942, 1170
Tru9I TTAA 7 cut(s) 123, 410, 449, 474, 924, 942, 1170
TscAI CASTG 3 cut(s) 773, 841, 1273
TseFI GTSAC 2 cut(s) 304, 795
TseI GCWGC 5 cut(s) 658, 1286, 1306, 1349, 1358
Tsp45I GTSAC 2 cut(s) 304, 795
TspDTI ATGAA 5 cut(s) 38, 141, 230, 584, 1212
TspGWI ACGGA 4 cut(s) 295, 299, 1168, 1323
TspRI CASTG 3 cut(s) 773, 841, 1273
Van91I CCANNNNNTGG 1 cut(s) 632
VspI ATTAAT 2 cut(s) 924, 1170
XapI RAATTY 2 cut(s) 45, 1082
XmiI GTMKAC 2 cut(s) 403, 415
XmnI GAANNNNTTC 2 cut(s) 112, 206
XspI CTAG 1 cut(s) 9
ZrmI AGTACT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.