Rorug07G0152300

Nnf1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010244
Physical Location & Seq
Reverse (-)
12078739 .. 12079221
483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0152300.1

Sequence Viewer

Length: 483 bp
ATGAGGGGTTTCCGAGATGCTTTAGGGTATGGGGACCTTCTGGATTTAGGTTTTCATGGACCTATGGCTACATGGTGGAATTCCGAAACACAGCTAAGGTTGGATAGGGCGGTTTGCACGCCATCTTGGTTCGATCTTTTTGGACACTCAAAACTGTTTCACCTATCTCCTTCGTATTCAGATCATGTTCCTATCCTGCTGAAGGCTAGTACAATACCTATAGCTACTAGGCCAAAATTTCATCGGTTCAAATTTGAGGCGTTTTGGGTACAACACTCTGAGTGTGCTGGGGTGGTTGATGAAGCATGGAGGACTGATGTAAGTGGTACTCCCATGTTTCGTGTAGCAAAGAAAATTGAGCATACTCGTATTACCTTAGATAGGTGGCAGAAACGGGTATTTAGAGGAAGACAAATCCAGATGCTTGGCATACGGGCTCGGTTAGAGCAGCTGCTTGATGTAACTATTTTCGAGGAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nnf1

Protein Analysis

160

Amino Acids

18.69

Weight (kDa)

9.34

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 110
AcsI RAATTY 3 cut(s) 79, 236, 251
AcuI CTGAAG 1 cut(s) 221
AfaI GTAC 3 cut(s) 211, 270, 328
AfiI CCNNNNNNNGG 2 cut(s) 202, 381
AgsI TTSAA 1 cut(s) 250
AluBI AGCT 3 cut(s) 94, 224, 451
AluI AGCT 3 cut(s) 94, 224, 451
AoxI GGCC 1 cut(s) 230
ApeKI GCWGC 2 cut(s) 448, 451
ApoI RAATTY 3 cut(s) 79, 236, 251
AspS9I GGNCC 2 cut(s) 34, 59
AsuHPI GGTGA 1 cut(s) 152
AvaII GGWCC 2 cut(s) 34, 59
BanII GRGCYC 1 cut(s) 439
BbsI GAAGAC 1 cut(s) 415
BbvI GCAGC 2 cut(s) 438, 460
BccI CCATC 1 cut(s) 130
BfaI CTAG 2 cut(s) 207, 228
BfmI CTRYAG 1 cut(s) 219
BisI GCNGC 2 cut(s) 449, 452
BlsI GCNGC 2 cut(s) 450, 453
Bme18I GGWCC 2 cut(s) 34, 59
BmgT120I GGNCC 2 cut(s) 34, 59
BmiI GGNNCC 1 cut(s) 35
BmsI GCATC 2 cut(s) 7, 411
BpiI GAAGAC 1 cut(s) 415
Bpu10I CCTNAGC 1 cut(s) 95
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 381
BseLI CCNNNNNNNGG 2 cut(s) 202, 381
BseMII CTCAG 1 cut(s) 270
BseXI GCAGC 2 cut(s) 438, 460
BseYI CCCAGC 1 cut(s) 287
BshFI GGCC 1 cut(s) 232
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 2 cut(s) 202, 381
BsmFI GGGAC 1 cut(s) 47
BsnI GGCC 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 2 cut(s) 133, 181
BspACI CCGC 1 cut(s) 110
BspANI GGCC 1 cut(s) 232
BspCNI CTCAG 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 35
BssMI GATC 2 cut(s) 133, 181
Bst4CI ACNGT 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 3 cut(s) 95, 279, 376
BstENI CCTNNNNNAGG 2 cut(s) 200, 379
BstKTI GATC 2 cut(s) 136, 184
BstMBI GATC 2 cut(s) 133, 181
BstSFI CTRYAG 1 cut(s) 219
BstV1I GCAGC 2 cut(s) 438, 460
BstV2I GAAGAC 1 cut(s) 415
BstXI CCANNNNNNTGG 1 cut(s) 425
BsuRI GGCC 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 34, 59
Csp6I GTAC 3 cut(s) 210, 269, 327
CviAII CATG 5 cut(s) 56, 72, 185, 306, 334
CviJI RGCY 7 cut(s) 68, 94, 206, 224, 232, 437, 451
CviKI_1 RGCY 7 cut(s) 68, 94, 206, 224, 232, 437, 451
CviQI GTAC 3 cut(s) 210, 269, 327
DdeI CTNAG 3 cut(s) 95, 279, 376
DpnI GATC 2 cut(s) 135, 183
DpnII GATC 2 cut(s) 133, 181
Eco24I GRGCYC 1 cut(s) 439
Eco47I GGWCC 2 cut(s) 34, 59
Eco57I CTGAAG 1 cut(s) 221
EcoNI CCTNNNNNAGG 2 cut(s) 200, 379
EcoO109I RGGNCCY 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 79
EcoT38I GRGCYC 1 cut(s) 439
FaeI CATG 5 cut(s) 59, 75, 188, 309, 337
FaqI GGGAC 1 cut(s) 47
FatI CATG 5 cut(s) 55, 71, 184, 305, 333
Fnu4HI GCNGC 2 cut(s) 449, 452
FriOI GRGCYC 1 cut(s) 439
Fsp4HI GCNGC 2 cut(s) 449, 452
FspBI CTAG 2 cut(s) 207, 228
GluI GCNGC 2 cut(s) 449, 452
GsaI CCCAGC 1 cut(s) 291
HaeIII GGCC 1 cut(s) 232
Hin1II CATG 5 cut(s) 59, 75, 188, 309, 337
HphI GGTGA 1 cut(s) 152
Hpy188I TCNGA 4 cut(s) 14, 85, 181, 280
Hpy188III TCNNGA 2 cut(s) 41, 418
HpyAV CCTTC 3 cut(s) 47, 180, 196
HpyCH4III ACNGT 1 cut(s) 156
HpyCH4V TGCA 1 cut(s) 117
HpyF3I CTNAG 3 cut(s) 95, 279, 376
Hsp92II CATG 5 cut(s) 59, 75, 188, 309, 337
Kzo9I GATC 2 cut(s) 133, 181
LpnPI CCDG 4 cut(s) 26, 209, 273, 431
Lsp1109I GCAGC 2 cut(s) 438, 460
LweI GCATC 2 cut(s) 7, 411
MaeI CTAG 2 cut(s) 207, 228
MaeIII GTNAC 1 cut(s) 460
MalI GATC 2 cut(s) 135, 183
MboI GATC 2 cut(s) 133, 181
MboII GAAGA 1 cut(s) 420
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 4 cut(s) 79, 236, 251, 354
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 5 cut(s) 250, 303, 398, 466, 469
MspA1I CMGCKG 1 cut(s) 451
NdeII GATC 2 cut(s) 133, 181
NlaIII CATG 5 cut(s) 59, 75, 188, 309, 337
NlaIV GGNNCC 1 cut(s) 35
PkrI GCNGC 2 cut(s) 450, 453
PpuMI RGGWCCY 1 cut(s) 34
Psp5II RGGWCCY 1 cut(s) 34
PspFI CCCAGC 1 cut(s) 287
PspN4I GGNNCC 1 cut(s) 35
PspPI GGNCC 2 cut(s) 34, 59
PspPPI RGGWCCY 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 451
RsaI GTAC 3 cut(s) 211, 270, 328
RsaNI GTAC 3 cut(s) 210, 269, 327
SatI GCNGC 2 cut(s) 449, 452
Sau3AI GATC 2 cut(s) 133, 181
Sau96I GGNCC 2 cut(s) 34, 59
SduI GDGCHC 1 cut(s) 439
SfaNI GCATC 2 cut(s) 7, 411
SfcI CTRYAG 1 cut(s) 219
SinI GGWCC 2 cut(s) 34, 59
Sse9I AATT 4 cut(s) 79, 236, 251, 354
SsiI CCGC 1 cut(s) 110
SspMI CTAG 2 cut(s) 207, 228
TaaI ACNGT 1 cut(s) 156
TaqI TCGA 2 cut(s) 132, 471
TasI AATT 4 cut(s) 79, 236, 251, 354
TatI WGTACW 1 cut(s) 209
TseI GCWGC 2 cut(s) 448, 451
TspDTI ATGAA 3 cut(s) 44, 230, 315
VpaK11BI GGWCC 2 cut(s) 34, 59
XagI CCTNNNNNAGG 2 cut(s) 200, 379
XapI RAATTY 3 cut(s) 79, 236, 251
XspI CTAG 2 cut(s) 207, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.