AT4G22220

Scaffold protein for the de novo synthesis of iron- sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic 2Fe-2S and 4Fe-4S proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016701
Physical Location & Seq
Reverse (-)
11759103 .. 11761057
1955 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22220.1

Sequence Viewer

Length: 504 bp
ATGATGCTCAAGCAAGCTGCGAAGAAGGCCCTAGGGCTTACGTCACGTCAATCAACACCGTGGTCTGTTGGTATCCTCCGAACCTACCATGAGAACGTCATCGACCACTACGATAATCCCCGCAACGTTGGGTCTTTCGATAAGAATGATCCCAACGTTGGTACGGGACTCGTAGGAGCTCCCGCTTGTGGTGATGTGATGAAGCTTCAAATCAAGGTCGATGAGAAGACTGGTCAAATCGTTGATGCTCGCTTTAAGACTTTTGGTTGTGGTTCAGCCATCGCTTCTTCATCTGTTGCCACTGAATGGGTGAAAGGCAAAGCTATGGAGGATGTTCTGACCATCAAAAACACCGAAATCGCAAAGCATCTTTCTCTCCCACCAGTCAAGCTCCACTGCAGTATGTTGGCGGAGGACGCTATCAAGGCAGCTGTGAAAGACTACAAGGAGAAGCGTGTGAAGACAAATGGTGCTGCAGCAGCAGGAGAAACCACACAGGCTTGA

Protein Analysis

167

Amino Acids

17.91

Weight (kDa)

9.1

Isoelectric Point (pI)

26.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU_N PF01592 28 - 153 1.2e-56 NifU-like N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016701)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22220
fragaria_vesca FvH4_5g05360
malus_domestica MD06G1173200.v1.1 MD14G1179700.v1.1
prunus_persica Prupe.5G172700_v2.0.a1
pyrus_communis pycom06g15430 pycom14g14950
rosa_chinensis RchiOBHm_Chr7g0193471
rosa_multiflora Rmu_sc0012783.1_g000005
rosa_roxburghii Rroxscaffold_3G00262070
rosa_rugosa Rorug07G0009600
rosa_samantha Rh7BG136700 Rh7DG137900
rosa_wichuraiana Rw7G011620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 306
AciI CCGC 3 cut(s) 121, 183, 410
AclI AACGTT 2 cut(s) 126, 156
AclWI GGATC 1 cut(s) 143
AfaI GTAC 1 cut(s) 163
AfiI CCNNNNNNNGG 3 cut(s) 158, 188, 306
AgsI TTSAA 1 cut(s) 209
AjiI CACGTC 1 cut(s) 47
AluBI AGCT 6 cut(s) 17, 179, 205, 323, 391, 431
AluI AGCT 6 cut(s) 17, 179, 205, 323, 391, 431
Alw21I GWGCWC 1 cut(s) 181
AlwI GGATC 1 cut(s) 143
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 5 cut(s) 17, 428, 473, 476, 479
AspA2I CCTAGG 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 2 cut(s) 203, 322
AvrII CCTAGG 1 cut(s) 31
BanII GRGCYC 1 cut(s) 181
BbsI GAAGAC 2 cut(s) 233, 467
Bbv12I GWGCWC 1 cut(s) 181
BbvI GCAGC 5 cut(s) 4, 440, 460, 488, 491
BccI CCATC 2 cut(s) 287, 350
BciVI GTATCC 1 cut(s) 83
BfaI CTAG 1 cut(s) 32
BfmI CTRYAG 2 cut(s) 397, 474
BfuI GTATCC 1 cut(s) 83
BisI GCNGC 5 cut(s) 18, 429, 474, 477, 480
BlnI CCTAGG 1 cut(s) 31
BlsI GCNGC 5 cut(s) 19, 430, 475, 478, 481
BmgBI CACGTC 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 28
BmsI GCATC 2 cut(s) 235, 376
BpiI GAAGAC 2 cut(s) 233, 467
BsaJI CCNNGG 2 cut(s) 31, 59
Bsc4I CCNNNNNNNGG 3 cut(s) 158, 188, 306
Bse1I ACTGG 2 cut(s) 235, 383
BseDI CCNNGG 2 cut(s) 31, 59
BseGI GGATG 1 cut(s) 337
BseLI CCNNNNNNNGG 3 cut(s) 158, 188, 306
BseNI ACTGG 2 cut(s) 235, 383
BseXI GCAGC 5 cut(s) 4, 440, 460, 488, 491
BshFI GGCC 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 181
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 3 cut(s) 158, 188, 306
BsmFI GGGAC 1 cut(s) 180
BsnI GGCC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 3 cut(s) 121, 183, 410
BspANI GGCC 1 cut(s) 29
BspMAI CTGCAG 2 cut(s) 401, 478
BspPI GGATC 1 cut(s) 143
BsrI ACTGG 2 cut(s) 235, 383
BssECI CCNNGG 2 cut(s) 31, 59
BssMI GATC 1 cut(s) 148
BssT1I CCWWGG 1 cut(s) 31
Bst4CI ACNGT 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 15, 250
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 1 cut(s) 337
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 4 cut(s) 26, 416, 425, 479
BstSFI CTRYAG 2 cut(s) 397, 474
BstV1I GCAGC 5 cut(s) 4, 440, 460, 488, 491
BstV2I GAAGAC 2 cut(s) 233, 467
BsuI GTATCC 1 cut(s) 83
BsuRI GGCC 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 59
BtgZI GCGATG 1 cut(s) 265
BtrI CACGTC 1 cut(s) 47
BtsCI GGATG 1 cut(s) 337
BtsI GCAGTG 1 cut(s) 394
BtsIMutI CAGTG 2 cut(s) 300, 394
Cac8I GCNNGC 2 cut(s) 15, 250
Cfr13I GGNCC 1 cut(s) 28
CseI GACGC 1 cut(s) 425
Csp6I GTAC 1 cut(s) 162
CviAII CATG 1 cut(s) 89
CviQI GTAC 1 cut(s) 162
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
EciI GGCGGA 1 cut(s) 425
Ecl136II GAGCTC 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 179
EcoICRI GAGCTC 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 1 cut(s) 92
FaiI YATR 3 cut(s) 90, 326, 404
FaqI GGGAC 1 cut(s) 180
FatI CATG 1 cut(s) 88
FauI CCCGC 2 cut(s) 128, 190
Fnu4HI GCNGC 5 cut(s) 18, 429, 474, 477, 480
FokI GGATG 1 cut(s) 344
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 5 cut(s) 18, 429, 474, 477, 480
FspBI CTAG 1 cut(s) 32
GluI GCNGC 5 cut(s) 18, 429, 474, 477, 480
HaeIII GGCC 1 cut(s) 29
HgaI GACGC 1 cut(s) 425
Hin1II CATG 1 cut(s) 92
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 1 cut(s) 168
HphI GGTGA 2 cut(s) 203, 322
Hpy188I TCNGA 2 cut(s) 80, 339
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 1 cut(s) 60
HpyCH4IV ACGT 5 cut(s) 41, 46, 96, 126, 156
HpyCH4V TGCA 2 cut(s) 399, 476
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 416, 425, 479
HpySE526I ACGT 5 cut(s) 41, 46, 96, 126, 156
Hsp92II CATG 1 cut(s) 92
Kzo9I GATC 1 cut(s) 148
LmnI GCTCC 3 cut(s) 176, 184, 396
LpnPI CCDG 4 cut(s) 216, 396, 468, 482
Lsp1109I GCAGC 5 cut(s) 4, 440, 460, 488, 491
LweI GCATC 2 cut(s) 235, 376
MaeI CTAG 1 cut(s) 32
MaeII ACGT 5 cut(s) 41, 46, 96, 126, 156
MaeIII GTNAC 1 cut(s) 42
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 4 cut(s) 34, 238, 279, 472
MhlI GDGCHC 1 cut(s) 181
MlyI GAGTC 1 cut(s) 162
MnlI CCTC 3 cut(s) 86, 322, 406
MseI TTAA 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 431
MwoI GCNNNNNNNGC 4 cut(s) 26, 416, 425, 479
NdeII GATC 1 cut(s) 148
NlaIII CATG 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PflMI CCANNNNNTGG 1 cut(s) 306
PkrI GCNGC 5 cut(s) 19, 430, 475, 478, 481
PleI GAGTC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 181
Psp1406I AACGTT 2 cut(s) 126, 156
PspPI GGNCC 1 cut(s) 28
PstI CTGCAG 2 cut(s) 401, 478
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
SacI GAGCTC 1 cut(s) 181
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 5 cut(s) 18, 429, 474, 477, 480
Sau3AI GATC 1 cut(s) 148
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 1 cut(s) 162
SduI GDGCHC 1 cut(s) 181
SfaNI GCATC 2 cut(s) 235, 376
SfcI CTRYAG 2 cut(s) 397, 474
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
SsiI CCGC 3 cut(s) 121, 183, 410
SspMI CTAG 1 cut(s) 32
SstI GAGCTC 1 cut(s) 181
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 1 cut(s) 60
TaiI ACGT 5 cut(s) 44, 49, 99, 129, 159
TaqI TCGA 3 cut(s) 102, 138, 219
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 2 cut(s) 307, 401
TseFI GTSAC 1 cut(s) 42
TseI GCWGC 5 cut(s) 17, 428, 473, 476, 479
Tsp45I GTSAC 1 cut(s) 42
TspDTI ATGAA 2 cut(s) 215, 279
TspRI CASTG 2 cut(s) 307, 401
Van91I CCANNNNNTGG 1 cut(s) 306
XmaJI CCTAGG 1 cut(s) 31
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.