MD06G1173200.v1.1

Scaffold protein for the de novo synthesis of iron- sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic 2Fe-2S and 4Fe-4S proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016701
Physical Location & Seq
Forward (+)
31313571 .. 31315743
2173 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1173200.v1.1.491

Sequence Viewer

Length: 522 bp
ATGTTGAGACTTGCGGCGAAGAGGCTTCTCCAAGGCGGCCGTCTGGGGTCACCGGAGGCTCAGTCGCCAGCGGTCCTGGGCCTGCCACGTATGTACCACGAGAGGGTGGTGGACCACTACGACAACCCCCGCAACGTAGGATCGTTCGATAAGAATGATCCGACGGTCGGCACGGGGCTCGTCGGCGCGCCAGCCTGTGGCGACGTGATGAAACTCCAGATTAAGGTCGACGACAGCACGGGAAAGATTGTTGATGCCTGCTTCAAGACCTTTGGGTGTGGATCGGCCATTGCTTCTTCTTCTGTCGCTACGGAATGGGTGAAGGGGAAACAAATGGAGGAAGTCCTGACTATTAAGAACACAGAGATTGCAAAACATCTTTCCCTACCTCCAGTTAAGCTTCACTGCAGCATGCTCGCGGAGGATGCCATCAAGGCAGCTGTTAAAGACTATCAAACCAAGCAAACCAAATCAAGTGGCAGTACAGAGACATCTCCTGTGGAGAAGGCTGCCAATGCTTGA

Protein Analysis

174

Amino Acids

18.43

Weight (kDa)

8.65

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU_N PF01592 31 - 157 5.2e-56 NifU-like N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016701)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22220
fragaria_vesca FvH4_5g05360
malus_domestica MD06G1173200.v1.1 MD14G1179700.v1.1
prunus_persica Prupe.5G172700_v2.0.a1
pyrus_communis pycom06g15430 pycom14g14950
rosa_chinensis RchiOBHm_Chr7g0193471
rosa_multiflora Rmu_sc0012783.1_g000005
rosa_roxburghii Rroxscaffold_3G00262070
rosa_rugosa Rorug07G0009600
rosa_samantha Rh7BG136700 Rh7DG137900
rosa_wichuraiana Rw7G011620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 197
AccI GTMKAC 1 cut(s) 228
AccII CGCG 2 cut(s) 188, 419
AciI CCGC 5 cut(s) 14, 36, 71, 130, 419
AclWI GGATC 3 cut(s) 148, 152, 289
AcoI YGGCCR 2 cut(s) 37, 285
AfaI GTAC 2 cut(s) 95, 484
AfiI CCNNNNNNNGG 4 cut(s) 103, 167, 197, 223
AgsI TTSAA 1 cut(s) 265
AjiI CACGTC 1 cut(s) 205
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 2 cut(s) 400, 440
AluI AGCT 2 cut(s) 400, 440
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 3 cut(s) 148, 152, 289
AoxI GGCC 3 cut(s) 37, 79, 285
ApeKI GCWGC 3 cut(s) 408, 437, 509
AscI GGCGCGCC 1 cut(s) 186
AspLEI GCGC 2 cut(s) 188, 190
AspS9I GGNCC 3 cut(s) 73, 79, 112
AsuHPI GGTGA 2 cut(s) 42, 331
AvaII GGWCC 2 cut(s) 73, 112
BaeI ACNNNNGTAYC 2 cut(s) 77, 110
BanII GRGCYC 1 cut(s) 180
BauI CACGAG 1 cut(s) 98
BbvI GCAGC 3 cut(s) 420, 449, 496
BccI CCATC 1 cut(s) 437
BceAI ACGGC 1 cut(s) 24
BcgI CGANNNNNNTGC 4 cut(s) 160, 194, 397, 431
BciT130I CCWGG 1 cut(s) 77
BcoDI GTCTC 1 cut(s) 482
BfmI CTRYAG 1 cut(s) 406
BglI GCCNNNNNGGC 1 cut(s) 434
BisI GCNGC 5 cut(s) 15, 37, 409, 438, 510
BlsI GCNGC 5 cut(s) 16, 38, 410, 439, 511
Bme1390I CCNGG 1 cut(s) 77
Bme18I GGWCC 2 cut(s) 73, 112
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 3 cut(s) 73, 79, 112
BmrFI CCNGG 1 cut(s) 77
BmsI GCATC 2 cut(s) 244, 415
BpmI CTGGAG 2 cut(s) 200, 375
BsaAI YACGTR 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 31, 76
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 4 cut(s) 103, 167, 197, 223
Bse1I ACTGG 1 cut(s) 392
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 2 cut(s) 31, 76
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 4 cut(s) 103, 167, 197, 223
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 392
BsePI GCGCGC 1 cut(s) 186
BseX3I CGGCCG 1 cut(s) 37
BseXI GCAGC 3 cut(s) 420, 449, 496
Bsh1236I CGCG 2 cut(s) 188, 419
Bsh1285I CGRYCG 2 cut(s) 40, 168
BshFI GGCC 3 cut(s) 39, 81, 287
BsiEI CGRYCG 2 cut(s) 40, 168
BsiSI CCGG 1 cut(s) 53
BslI CCNNNNNNNGG 4 cut(s) 103, 167, 197, 223
BsmAI GTCTC 1 cut(s) 482
BsnI GGCC 3 cut(s) 39, 81, 287
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 3 cut(s) 140, 157, 281
BspACI CCGC 5 cut(s) 14, 36, 71, 130, 419
BspANI GGCC 3 cut(s) 39, 81, 287
BspCNI CTCAG 1 cut(s) 73
BspFNI CGCG 2 cut(s) 188, 419
BspMAI CTGCAG 1 cut(s) 410
BspPI GGATC 3 cut(s) 148, 152, 289
BsrDI GCAATG 1 cut(s) 288
BsrI ACTGG 1 cut(s) 392
BssECI CCNNGG 2 cut(s) 31, 76
BssHII GCGCGC 1 cut(s) 186
BssMI GATC 3 cut(s) 140, 157, 281
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 166
Bst6I CTCTTC 1 cut(s) 14
BstBAI YACGTR 1 cut(s) 89
BstC8I GCNNGC 7 cut(s) 69, 83, 188, 192, 259, 413, 417
BstDEI CTNAG 1 cut(s) 60
BstEII GGTNACC 1 cut(s) 48
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 2 cut(s) 188, 419
BstHHI GCGC 2 cut(s) 188, 190
BstKTI GATC 3 cut(s) 143, 160, 284
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 3 cut(s) 140, 157, 281
BstMCI CGRYCG 2 cut(s) 40, 168
BstMWI GCNNNNNNNGC 3 cut(s) 425, 434, 515
BstNI CCWGG 1 cut(s) 77
BstNSI RCATGY 1 cut(s) 415
BstPI GGTNACC 1 cut(s) 48
BstSCI CCNGG 1 cut(s) 75
BstSFI CTRYAG 1 cut(s) 406
BstUI CGCG 2 cut(s) 188, 419
BstV1I GCAGC 3 cut(s) 420, 449, 496
BstZI CGGCCG 1 cut(s) 37
BsuRI GGCC 3 cut(s) 39, 81, 287
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 403
Cac8I GCNNGC 7 cut(s) 69, 83, 188, 192, 259, 413, 417
CfoI GCGC 2 cut(s) 188, 190
Cfr13I GGNCC 3 cut(s) 73, 79, 112
Csp6I GTAC 2 cut(s) 94, 483
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 1 cut(s) 412
CviQI GTAC 2 cut(s) 94, 483
DdeI CTNAG 1 cut(s) 60
DpnI GATC 3 cut(s) 142, 159, 283
DpnII GATC 3 cut(s) 140, 157, 281
EaeI YGGCCR 2 cut(s) 37, 285
EagI CGGCCG 1 cut(s) 37
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
EclXI CGGCCG 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 73, 112
Eco52I CGGCCG 1 cut(s) 37
Eco91I GGTNACC 1 cut(s) 48
EcoO65I GGTNACC 1 cut(s) 48
EcoRII CCWGG 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 1 cut(s) 415
FaiI YATR 2 cut(s) 92, 413
FatI CATG 1 cut(s) 411
FauI CCCGC 1 cut(s) 137
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 5 cut(s) 15, 37, 409, 438, 510
FokI GGATG 1 cut(s) 437
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 5 cut(s) 15, 37, 409, 438, 510
GlaI GCGC 2 cut(s) 187, 189
GluI GCNGC 5 cut(s) 15, 37, 409, 438, 510
GsuI CTGGAG 2 cut(s) 200, 375
HaeIII GGCC 3 cut(s) 39, 81, 287
HapII CCGG 1 cut(s) 53
HhaI GCGC 2 cut(s) 188, 190
Hin1II CATG 1 cut(s) 415
Hin6I GCGC 2 cut(s) 186, 188
HinP1I GCGC 2 cut(s) 186, 188
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 398
HpaII CCGG 1 cut(s) 53
HphI GGTGA 2 cut(s) 42, 331
Hpy166II GTNNAC 2 cut(s) 112, 229
Hpy188I TCNGA 1 cut(s) 162
Hpy188III TCNNGA 3 cut(s) 217, 265, 346
Hpy8I GTNNAC 2 cut(s) 112, 229
Hpy99I CGWCG 4 cut(s) 166, 185, 206, 233
HpyAV CCTTC 2 cut(s) 316, 499
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 3 cut(s) 88, 135, 204
HpyCH4V TGCA 2 cut(s) 371, 408
HpyF10VI GCNNNNNNNGC 3 cut(s) 425, 434, 515
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 3 cut(s) 88, 135, 204
Hsp92II CATG 1 cut(s) 415
HspAI GCGC 2 cut(s) 186, 188
Kzo9I GATC 3 cut(s) 140, 157, 281
Lsp1109I GCAGC 3 cut(s) 420, 449, 496
LweI GCATC 2 cut(s) 244, 415
MaeII ACGT 3 cut(s) 88, 135, 204
MaeIII GTNAC 1 cut(s) 48
MalI GATC 3 cut(s) 142, 159, 283
MboI GATC 3 cut(s) 140, 157, 281
MboII GAAGA 3 cut(s) 31, 288, 291
MhlI GDGCHC 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 6 cut(s) 15, 49, 96, 331, 399, 415
MseI TTAA 4 cut(s) 222, 354, 396, 444
MspA1I CMGCKG 2 cut(s) 71, 440
MspI CCGG 1 cut(s) 53
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
MvnI CGCG 2 cut(s) 188, 419
MwoI GCNNNNNNNGC 3 cut(s) 425, 434, 515
NdeII GATC 3 cut(s) 140, 157, 281
NlaIII CATG 1 cut(s) 415
NmuCI GTSAC 1 cut(s) 48
NspI RCATGY 1 cut(s) 415
PaeI GCATGC 1 cut(s) 415
PalAI GGCGCGCC 1 cut(s) 186
PauI GCGCGC 1 cut(s) 186
PflMI CCANNNNNTGG 1 cut(s) 197
PkrI GCNGC 5 cut(s) 16, 38, 410, 439, 511
Ppu21I YACGTR 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 75
PspEI GGTNACC 1 cut(s) 48
PspGI CCWGG 1 cut(s) 75
PspPI GGNCC 3 cut(s) 73, 79, 112
PstI CTGCAG 1 cut(s) 410
PteI GCGCGC 1 cut(s) 186
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 2 cut(s) 95, 484
RsaNI GTAC 2 cut(s) 94, 483
SalI GTCGAC 1 cut(s) 227
SaqAI TTAA 4 cut(s) 222, 354, 396, 444
SatI GCNGC 5 cut(s) 15, 37, 409, 438, 510
Sau3AI GATC 3 cut(s) 140, 157, 281
Sau96I GGNCC 3 cut(s) 73, 79, 112
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 1 cut(s) 180
SetI ASST 8 cut(s) 91, 138, 207, 228, 272, 391, 402, 442
SfaNI GCATC 2 cut(s) 244, 415
SfcI CTRYAG 1 cut(s) 406
SgsI GGCGCGCC 1 cut(s) 186
SinI GGWCC 2 cut(s) 73, 112
SphI GCATGC 1 cut(s) 415
SsiI CCGC 5 cut(s) 14, 36, 71, 130, 419
StyD4I CCNGG 1 cut(s) 75
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 3 cut(s) 91, 138, 207
TaqI TCGA 2 cut(s) 147, 228
TatI WGTACW 1 cut(s) 482
TauI GCSGC 2 cut(s) 17, 39
Tru1I TTAA 4 cut(s) 222, 354, 396, 444
Tru9I TTAA 4 cut(s) 222, 354, 396, 444
TscAI CASTG 1 cut(s) 410
TseFI GTSAC 1 cut(s) 48
TseI GCWGC 3 cut(s) 408, 437, 509
Tsp45I GTSAC 1 cut(s) 48
TspDTI ATGAA 1 cut(s) 224
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 1 cut(s) 410
Van91I CCANNNNNTGG 1 cut(s) 197
VpaK11BI GGWCC 2 cut(s) 73, 112
XceI RCATGY 1 cut(s) 415
XmiI GTMKAC 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.