Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0016701

Physical Location & Seq

Genomic Coordinates Forward (+)

27244449 .. 27246583

Gene Structure 2135 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1179700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 522 bp

ATGTTGAGACTTGCGGCGAAGAGGATTCTCCAAGGCGGCCGTCTGGGCTCGCCGGAGGCTCAGTCACCGGCGGTCCTGAGCCTGCCACGTATGTACCACGAGAGGGTGGTGGACCACTACGACAACCCCCGCAACGTCGGAACGTTCGACAAGAATGACCCGACGATCGGCACGGGGCTCGTTGGCGCTCCAGCCTGTGGCGACGTGATGAAGCTCCAGATTAAGGTCGATGACAGCACGGGAAAGATTGTTGATGCTTGCTTCAAGACCTTTGGGTGTGGATCGGCCATTGCTTCTTCTTCTGTCGCTACGGAATGGGTGAAGGGGAAACAAATGGAGGAAGTCTTGACGATTAAGAACACAGAGATTGCAAAACATCTTTCACTTCCTCCAGTGAAGCTTCACTGCAGCATGCTCGCCGAGGATGCCATTAAGGCAGCTGTTAAAGACTATCAAACCAAGCAAACCAAAAAAACTGGCGGTACAGAGGCATCTCCTGCGGAGAAGGCTGTCAATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005198 GO:0005488 GO:0005506 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006807 GO:0006873 GO:0006875 GO:0006879 GO:0008150 GO:0008152 GO:0008198 GO:0009987 GO:0010467 GO:0016740 GO:0016782 GO:0019538 GO:0019725 GO:0030003 GO:0031974 GO:0036455 GO:0042592 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048037 GO:0048878 GO:0050801 GO:0051536 GO:0051537 GO:0051539 GO:0051540 GO:0051604 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0097428 GO:0098771 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NifU_N

Protein Analysis

174

Amino Acids

18.48

Weight (kDa)

8.86

Isoelectric Point (pI)

49.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NifU_N	PF01592	31 - 157	5.9e-56	NifU-like N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016701)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22220
fragaria_vesca	FvH4_5g05360
malus_domestica	MD06G1173200.v1.1 MD14G1179700.v1.1
prunus_persica	Prupe.5G172700_v2.0.a1
pyrus_communis	pycom06g15430 pycom14g14950
rosa_chinensis	RchiOBHm_Chr7g0193471
rosa_multiflora	Rmu_sc0012783.1_g000005
rosa_roxburghii	Rroxscaffold_3G00262070
rosa_rugosa	Rorug07G0009600
rosa_samantha	Rh7BG136700 Rh7DG137900
rosa_wichuraiana	Rw7G011620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	197
AciI	CCGC	6 cut(s)	14, 36, 71, 130, 480, 500
AclI	AACGTT	1 cut(s)	143
AclWI	GGATC	1 cut(s)	289
AcoI	YGGCCR	2 cut(s)	37, 285
AfaI	GTAC	2 cut(s)	95, 484
AfiI	CCNNNNNNNGG	4 cut(s)	103, 167, 197, 223
AgsI	TTSAA	1 cut(s)	265
AjiI	CACGTC	1 cut(s)	205
AluBI	AGCT	3 cut(s)	214, 400, 440
AluI	AGCT	3 cut(s)	214, 400, 440
AlwI	GGATC	1 cut(s)	289
AoxI	GGCC	2 cut(s)	37, 285
ApeKI	GCWGC	2 cut(s)	408, 437
AspLEI	GCGC	1 cut(s)	188
AspS9I	GGNCC	2 cut(s)	73, 112
AsuHPI	GGTGA	2 cut(s)	57, 331
AvaII	GGWCC	2 cut(s)	73, 112
BaeI	ACNNNNGTAYC	2 cut(s)	77, 110
BanII	GRGCYC	2 cut(s)	50, 180
BauI	CACGAG	1 cut(s)	98
BbvI	GCAGC	2 cut(s)	420, 449
BceAI	ACGGC	1 cut(s)	24
BcgI	CGANNNNNNTGC	4 cut(s)	160, 194, 397, 431
BfmI	CTRYAG	1 cut(s)	406
BfoI	RGCGCY	1 cut(s)	189
BglI	GCCNNNNNGGC	2 cut(s)	45, 434
BisI	GCNGC	4 cut(s)	15, 37, 409, 438
BlsI	GCNGC	4 cut(s)	16, 38, 410, 439
Bme18I	GGWCC	2 cut(s)	73, 112
BmgBI	CACGTC	1 cut(s)	205
BmgT120I	GGNCC	2 cut(s)	73, 112
BmsI	GCATC	3 cut(s)	244, 415, 500
BpmI	CTGGAG	3 cut(s)	174, 200, 375
Bpu10I	CCTNAGC	1 cut(s)	77
BsaAI	YACGTR	1 cut(s)	89
BsaJI	CCNNGG	2 cut(s)	31, 420
BsaXI	ACNNNNNCTCC	2 cut(s)	47, 77
Bsc4I	CCNNNNNNNGG	4 cut(s)	103, 167, 197, 223
Bse118I	RCCGGY	1 cut(s)	67
Bse1I	ACTGG	2 cut(s)	392, 481
Bse3DI	GCAATG	1 cut(s)	288
BseDI	CCNNGG	2 cut(s)	31, 420
BseGI	GGATG	1 cut(s)	430
BseLI	CCNNNNNNNGG	4 cut(s)	103, 167, 197, 223
BseMI	GCAATG	1 cut(s)	288
BseMII	CTCAG	2 cut(s)	68, 74
BseNI	ACTGG	2 cut(s)	392, 481
BseX3I	CGGCCG	1 cut(s)	37
BseXI	GCAGC	2 cut(s)	420, 449
Bsh1285I	CGRYCG	2 cut(s)	40, 168
BshFI	GGCC	2 cut(s)	39, 287
BsiEI	CGRYCG	2 cut(s)	40, 168
BsiSI	CCGG	2 cut(s)	53, 68
BslI	CCNNNNNNNGG	4 cut(s)	103, 167, 197, 223
BsnI	GGCC	2 cut(s)	39, 287
Bsp1286I	GDGCHC	2 cut(s)	50, 180
Bsp143I	GATC	2 cut(s)	165, 281
BspACI	CCGC	6 cut(s)	14, 36, 71, 130, 480, 500
BspANI	GGCC	2 cut(s)	39, 287
BspCNI	CTCAG	2 cut(s)	69, 73
BspMAI	CTGCAG	1 cut(s)	410
BspPI	GGATC	1 cut(s)	289
BsrDI	GCAATG	1 cut(s)	288
BsrFI	RCCGGY	1 cut(s)	67
BsrI	ACTGG	2 cut(s)	392, 481
BssAI	RCCGGY	1 cut(s)	67
BssECI	CCNNGG	2 cut(s)	31, 420
BssMI	GATC	2 cut(s)	165, 281
BssSI	CACGAG	1 cut(s)	98
BssT1I	CCWWGG	1 cut(s)	31
Bst2BI	CACGAG	1 cut(s)	98
Bst6I	CTCTTC	1 cut(s)	14
BstAPI	GCANNNNNTGC	1 cut(s)	497
BstBAI	YACGTR	1 cut(s)	89
BstC8I	GCNNGC	5 cut(s)	50, 83, 259, 413, 417
BstDEI	CTNAG	2 cut(s)	60, 77
BstF5I	GGATG	1 cut(s)	430
BstH2I	RGCGCY	1 cut(s)	189
BstHHI	GCGC	1 cut(s)	188
BstKTI	GATC	2 cut(s)	168, 284
BstMBI	GATC	2 cut(s)	165, 281
BstMCI	CGRYCG	2 cut(s)	40, 168
BstMWI	GCNNNNNNNGC	6 cut(s)	45, 425, 434, 497, 506, 515
BstNSI	RCATGY	1 cut(s)	415
BstSFI	CTRYAG	1 cut(s)	406
BstV1I	GCAGC	2 cut(s)	420, 449
BstZI	CGGCCG	1 cut(s)	37
BsuRI	GGCC	2 cut(s)	39, 287
BtrI	CACGTC	1 cut(s)	205
BtsCI	GGATG	1 cut(s)	430
BtsI	GCAGTG	1 cut(s)	403
BtsIMutI	CAGTG	2 cut(s)	399, 403
Cac8I	GCNNGC	5 cut(s)	50, 83, 259, 413, 417
CfoI	GCGC	1 cut(s)	188
Cfr10I	RCCGGY	1 cut(s)	67
Cfr13I	GGNCC	2 cut(s)	73, 112
Csp6I	GTAC	2 cut(s)	94, 483
CviAII	CATG	1 cut(s)	412
CviQI	GTAC	2 cut(s)	94, 483
DdeI	CTNAG	2 cut(s)	60, 77
DpnI	GATC	2 cut(s)	167, 283
DpnII	GATC	2 cut(s)	165, 281
EaeI	YGGCCR	2 cut(s)	37, 285
EagI	CGGCCG	1 cut(s)	37
Eam1104I	CTCTTC	1 cut(s)	14
EarI	CTCTTC	1 cut(s)	14
EclXI	CGGCCG	1 cut(s)	37
Eco130I	CCWWGG	1 cut(s)	31
Eco24I	GRGCYC	2 cut(s)	50, 180
Eco47I	GGWCC	2 cut(s)	73, 112
Eco52I	CGGCCG	1 cut(s)	37
EcoT14I	CCWWGG	1 cut(s)	31
EcoT38I	GRGCYC	2 cut(s)	50, 180
ErhI	CCWWGG	1 cut(s)	31
FaeI	CATG	1 cut(s)	415
FaiI	YATR	2 cut(s)	92, 413
FatI	CATG	1 cut(s)	411
FauI	CCCGC	1 cut(s)	137
Fnu4HI	GCNGC	4 cut(s)	15, 37, 409, 438
FokI	GGATG	1 cut(s)	437
FriOI	GRGCYC	2 cut(s)	50, 180
Fsp4HI	GCNGC	4 cut(s)	15, 37, 409, 438
GlaI	GCGC	1 cut(s)	187
GluI	GCNGC	4 cut(s)	15, 37, 409, 438
GsuI	CTGGAG	3 cut(s)	174, 200, 375
HaeII	RGCGCY	1 cut(s)	189
HaeIII	GGCC	2 cut(s)	39, 287
HapII	CCGG	2 cut(s)	53, 68
HhaI	GCGC	1 cut(s)	188
Hin1II	CATG	1 cut(s)	415
Hin6I	GCGC	1 cut(s)	186
HinP1I	GCGC	1 cut(s)	186
HindIII	AAGCTT	1 cut(s)	398
HinfI	GANTC	1 cut(s)	25
HpaII	CCGG	2 cut(s)	53, 68
HphI	GGTGA	2 cut(s)	57, 331
Hpy166II	GTNNAC	1 cut(s)	112
Hpy188I	TCNGA	1 cut(s)	140
Hpy188III	TCNNGA	4 cut(s)	76, 217, 265, 346
Hpy8I	GTNNAC	1 cut(s)	112
Hpy99I	CGWCG	3 cut(s)	140, 166, 206
HpyAV	CCTTC	2 cut(s)	316, 499
HpyCH4IV	ACGT	4 cut(s)	88, 135, 143, 204
HpyCH4V	TGCA	2 cut(s)	371, 408
HpyF10VI	GCNNNNNNNGC	6 cut(s)	45, 425, 434, 497, 506, 515
HpyF3I	CTNAG	2 cut(s)	60, 77
HpySE526I	ACGT	4 cut(s)	88, 135, 143, 204
Hsp92II	CATG	1 cut(s)	415
HspAI	GCGC	1 cut(s)	186
Kzo9I	GATC	2 cut(s)	165, 281
LmnI	GCTCC	2 cut(s)	193, 219
Lsp1109I	GCAGC	2 cut(s)	420, 449
LweI	GCATC	3 cut(s)	244, 415, 500
MaeII	ACGT	4 cut(s)	88, 135, 143, 204
MaeIII	GTNAC	1 cut(s)	63
MalI	GATC	2 cut(s)	167, 283
MboI	GATC	2 cut(s)	165, 281
MboII	GAAGA	3 cut(s)	31, 288, 291
MhlI	GDGCHC	2 cut(s)	50, 180
MmeI	TCCRAC	1 cut(s)	118
MnlI	CCTC	7 cut(s)	15, 49, 96, 331, 399, 415, 481
MseI	TTAA	4 cut(s)	222, 354, 432, 444
MspA1I	CMGCKG	1 cut(s)	440
MspI	CCGG	2 cut(s)	53, 68
MwoI	GCNNNNNNNGC	6 cut(s)	45, 425, 434, 497, 506, 515
NdeII	GATC	2 cut(s)	165, 281
NlaIII	CATG	1 cut(s)	415
NmeAIII	GCCGAG	1 cut(s)	445
NmuCI	GTSAC	1 cut(s)	63
NspI	RCATGY	1 cut(s)	415
PaeI	GCATGC	1 cut(s)	415
PcsI	WCGNNNNNNNCGW	1 cut(s)	144
PfeI	GAWTC	1 cut(s)	25
PflMI	CCANNNNNTGG	1 cut(s)	197
PkrI	GCNGC	4 cut(s)	16, 38, 410, 439
Ple19I	CGATCG	1 cut(s)	168
Ppu21I	YACGTR	1 cut(s)	89
Psp1406I	AACGTT	1 cut(s)	143
PspPI	GGNCC	2 cut(s)	73, 112
PstI	CTGCAG	1 cut(s)	410
PvuI	CGATCG	1 cut(s)	168
PvuII	CAGCTG	1 cut(s)	440
RsaI	GTAC	2 cut(s)	95, 484
RsaNI	GTAC	2 cut(s)	94, 483
SaqAI	TTAA	4 cut(s)	222, 354, 432, 444
SatI	GCNGC	4 cut(s)	15, 37, 409, 438
Sau3AI	GATC	2 cut(s)	165, 281
Sau96I	GGNCC	2 cut(s)	73, 112
SduI	GDGCHC	2 cut(s)	50, 180
SetI	ASST	9 cut(s)	91, 138, 146, 207, 216, 228, 272, 402, 442
SfaNI	GCATC	3 cut(s)	244, 415, 500
SfcI	CTRYAG	1 cut(s)	406
SgrAI	CRCCGGYG	1 cut(s)	67
SinI	GGWCC	2 cut(s)	73, 112
SphI	GCATGC	1 cut(s)	415
SsiI	CCGC	6 cut(s)	14, 36, 71, 130, 480, 500
StyI	CCWWGG	1 cut(s)	31
TaiI	ACGT	4 cut(s)	91, 138, 146, 207
TaqI	TCGA	2 cut(s)	147, 228
TauI	GCSGC	2 cut(s)	17, 39
TfiI	GAWTC	1 cut(s)	25
Tru1I	TTAA	4 cut(s)	222, 354, 432, 444
Tru9I	TTAA	4 cut(s)	222, 354, 432, 444
TscAI	CASTG	2 cut(s)	399, 410
TseFI	GTSAC	1 cut(s)	63
TseI	GCWGC	2 cut(s)	408, 437
Tsp45I	GTSAC	1 cut(s)	63
TspDTI	ATGAA	1 cut(s)	224
TspGWI	ACGGA	1 cut(s)	326
TspRI	CASTG	2 cut(s)	399, 410
Van91I	CCANNNNNTGG	1 cut(s)	197
VpaK11BI	GGWCC	2 cut(s)	73, 112
XceI	RCATGY	1 cut(s)	415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.