Rroxscaffold_3G00262070

Scaffold protein for the de novo synthesis of iron- sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic 2Fe-2S and 4Fe-4S proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016701
Physical Location & Seq
Reverse (-)
56182083 .. 56183797
1715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262070.1

Sequence Viewer

Length: 510 bp
ATGTTGAGTCTGGTATCGAAGAGACTGCTCCAAGGGTCCCGCGAGGCCCAGATGCCGGCGGCCCTGGCTCTGCCGCGTCTCTACCACGAGAGGGTGGTCGACCACTACGACAACCCCCGCAACGTCGGCTCCTTCGACAAAAACGACCCCTCCGTCGGCACCGGACTCGTCGGCGCACCCGCCTGCGGTGACGTCATGAAGCTCCAGATTAAGGTCGGCGAAGATGGCAAGATTACTGACGCCGTCTTCAAGACCTTTGGCTGTGGATCCGCCATCGCCTCCTCTTCCGTCGCTACTGAATGGGTGAAGGGGAAGCAAATGGAGGAGGTCCTGACCATTAAGAACACGGAAATTGCGAAACATCTCTCTCTTCCTCCAGTCAAGCTACACTGCAGCATGCTTGCTGAGGATGCCATCAAGGCAGCTGTTAAAGACTATCAAACAAAGCTGGCTAAATCATCAAATGGCAGCTCAGAGGCCTCACCTGTGGAGAAGGCTGCTAATGCTTGA

Protein Analysis

169

Amino Acids

17.98

Weight (kDa)

8.39

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NifU_N PF01592 28 - 150 7e-53 NifU-like N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016701)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22220
fragaria_vesca FvH4_5g05360
malus_domestica MD06G1173200.v1.1 MD14G1179700.v1.1
prunus_persica Prupe.5G172700_v2.0.a1
pyrus_communis pycom06g15430 pycom14g14950
rosa_chinensis RchiOBHm_Chr7g0193471
rosa_multiflora Rmu_sc0012783.1_g000005
rosa_roxburghii Rroxscaffold_3G00262070
rosa_rugosa Rorug07G0009600
rosa_samantha Rh7BG136700 Rh7DG137900
rosa_wichuraiana Rw7G011620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
AatII GACGTC 1 cut(s) 195
AccB1I GGYRCC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 99
AccII CGCG 2 cut(s) 42, 76
AciI CCGC 7 cut(s) 40, 59, 74, 118, 180, 186, 270
AclWI GGATC 2 cut(s) 261, 274
AcyI GRCGYC 2 cut(s) 192, 240
AfiI CCNNNNNNNGG 5 cut(s) 55, 91, 155, 185, 211
AgsI TTSAA 1 cut(s) 250
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 5 cut(s) 202, 385, 425, 448, 471
AluI AGCT 5 cut(s) 202, 385, 425, 448, 471
Alw26I GTCTC 2 cut(s) 16, 83
AlwI GGATC 2 cut(s) 261, 274
AoxI GGCC 3 cut(s) 45, 60, 477
ApeKI GCWGC 4 cut(s) 393, 422, 468, 497
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 4 cut(s) 36, 46, 61, 328
AsuHPI GGTGA 3 cut(s) 200, 316, 474
AvaII GGWCC 2 cut(s) 36, 328
BamHI GGATCC 1 cut(s) 266
BanI GGYRCC 1 cut(s) 158
BauI CACGAG 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 238
BbvCI CCTCAGC 1 cut(s) 405
BbvI GCAGC 4 cut(s) 405, 434, 480, 484
BccI CCATC 3 cut(s) 218, 281, 422
BceAI ACGGC 1 cut(s) 227
BcgI CGANNNNNNTGC 4 cut(s) 7, 41, 148, 182
BciT130I CCWGG 1 cut(s) 65
BcoDI GTCTC 2 cut(s) 16, 83
BfmI CTRYAG 1 cut(s) 391
BglI GCCNNNNNGGC 1 cut(s) 419
BisI GCNGC 6 cut(s) 60, 74, 394, 423, 469, 498
BlsI GCNGC 6 cut(s) 61, 75, 395, 424, 470, 499
Bme1390I CCNGG 1 cut(s) 65
Bme18I GGWCC 2 cut(s) 36, 328
BmgT120I GGNCC 4 cut(s) 36, 46, 61, 328
BmiI GGNNCC 5 cut(s) 37, 38, 130, 160, 268
BmrFI CCNGG 1 cut(s) 65
BmsI GCATC 2 cut(s) 42, 400
BpiI GAAGAC 1 cut(s) 238
BpmI CTGGAG 2 cut(s) 188, 360
Bpu10I CCTNAGC 1 cut(s) 405
BsaHI GRCGYC 2 cut(s) 192, 240
BsaJI CCNNGG 2 cut(s) 31, 63
BsaWI WCCGGW 1 cut(s) 161
BsaXI ACNNNNNCTCC 4 cut(s) 113, 134, 143, 164
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 91, 155, 185, 211
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 377
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 31, 63
BseGI GGATG 1 cut(s) 415
BseLI CCNNNNNNNGG 5 cut(s) 55, 91, 155, 185, 211
BseMII CTCAG 2 cut(s) 396, 486
BseNI ACTGG 1 cut(s) 377
BseRI GAGGAG 2 cut(s) 271, 338
BseXI GCAGC 4 cut(s) 405, 434, 480, 484
Bsh1236I CGCG 2 cut(s) 42, 76
BshFI GGCC 3 cut(s) 47, 62, 479
BshNI GGYRCC 1 cut(s) 158
BsiSI CCGG 2 cut(s) 56, 162
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 5 cut(s) 55, 91, 155, 185, 211
BsmAI GTCTC 2 cut(s) 16, 83
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 3 cut(s) 47, 62, 479
Bsp143I GATC 1 cut(s) 266
BspACI CCGC 7 cut(s) 40, 59, 74, 118, 180, 186, 270
BspANI GGCC 3 cut(s) 47, 62, 479
BspCNI CTCAG 2 cut(s) 397, 485
BspFNI CGCG 2 cut(s) 42, 76
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 5 cut(s) 37, 38, 130, 160, 268
BspMAI CTGCAG 1 cut(s) 395
BspPI GGATC 2 cut(s) 261, 274
BspT107I GGYRCC 1 cut(s) 158
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 1 cut(s) 377
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 31, 63
BssMI GATC 1 cut(s) 266
BssNI GRCGYC 2 cut(s) 192, 240
BssSI CACGAG 1 cut(s) 86
BssT1I CCWWGG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 86
Bst2UI CCWGG 1 cut(s) 65
Bst6I CTCTTC 3 cut(s) 14, 289, 375
BstACI GRCGYC 2 cut(s) 192, 240
BstC8I GCNNGC 5 cut(s) 57, 184, 398, 402, 450
BstDEI CTNAG 2 cut(s) 405, 472
BstF5I GGATG 1 cut(s) 415
BstFNI CGCG 2 cut(s) 42, 76
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 1 cut(s) 269
BstMAI GTCTC 2 cut(s) 16, 83
BstMBI GATC 1 cut(s) 266
BstMWI GCNNNNNNNGC 6 cut(s) 65, 126, 225, 410, 419, 503
BstNI CCWGG 1 cut(s) 65
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 63
BstSFI CTRYAG 1 cut(s) 391
BstUI CGCG 2 cut(s) 42, 76
BstV1I GCAGC 4 cut(s) 405, 434, 480, 484
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 1 cut(s) 266
BstYI RGATCY 1 cut(s) 266
BsuRI GGCC 3 cut(s) 47, 62, 479
BtgZI GCGATG 1 cut(s) 259
BtsCI GGATG 1 cut(s) 415
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 388
Cac8I GCNNGC 5 cut(s) 57, 184, 398, 402, 450
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 4 cut(s) 36, 46, 61, 328
CseI GACGC 2 cut(s) 65, 248
CviAII CATG 2 cut(s) 196, 397
DdeI CTNAG 2 cut(s) 405, 472
DpnI GATC 1 cut(s) 268
DpnII GATC 1 cut(s) 266
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
Eam1104I CTCTTC 3 cut(s) 14, 289, 375
EarI CTCTTC 3 cut(s) 14, 289, 375
EciI GGCGGA 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 31
Eco147I AGGCCT 1 cut(s) 479
Eco47I GGWCC 2 cut(s) 36, 328
EcoO109I RGGNCCY 2 cut(s) 36, 328
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 2 cut(s) 199, 400
FaiI YATR 2 cut(s) 197, 398
FaqI GGGAC 1 cut(s) 22
FatI CATG 2 cut(s) 195, 396
FauI CCCGC 3 cut(s) 47, 125, 187
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 6 cut(s) 60, 74, 394, 423, 469, 498
FokI GGATG 1 cut(s) 422
Fsp4HI GCNGC 6 cut(s) 60, 74, 394, 423, 469, 498
GlaI GCGC 1 cut(s) 175
GluI GCNGC 6 cut(s) 60, 74, 394, 423, 469, 498
GsuI CTGGAG 2 cut(s) 188, 360
HaeIII GGCC 3 cut(s) 47, 62, 479
HapII CCGG 2 cut(s) 56, 162
HgaI GACGC 2 cut(s) 65, 248
HhaI GCGC 1 cut(s) 176
Hin1I GRCGYC 2 cut(s) 192, 240
Hin1II CATG 2 cut(s) 199, 400
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 2 cut(s) 7, 165
HpaII CCGG 2 cut(s) 56, 162
HphI GGTGA 3 cut(s) 200, 316, 474
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 1 cut(s) 475
Hpy188III TCNNGA 4 cut(s) 196, 205, 250, 331
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 4 cut(s) 128, 158, 173, 293
HpyAV CCTTC 3 cut(s) 142, 301, 487
HpyCH4IV ACGT 2 cut(s) 123, 192
HpyCH4V TGCA 1 cut(s) 393
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 126, 225, 410, 419, 503
HpyF3I CTNAG 2 cut(s) 405, 472
HpySE526I ACGT 2 cut(s) 123, 192
Hsp92I GRCGYC 2 cut(s) 192, 240
Hsp92II CATG 2 cut(s) 199, 400
HspAI GCGC 1 cut(s) 174
KflI GGGWCCC 1 cut(s) 36
KroI GCCGGC 1 cut(s) 55
KroNI GCCGGC 1 cut(s) 57
Kzo9I GATC 1 cut(s) 266
LmnI GCTCC 3 cut(s) 33, 134, 207
Lsp1109I GCAGC 4 cut(s) 405, 434, 480, 484
LweI GCATC 2 cut(s) 42, 400
MaeII ACGT 2 cut(s) 123, 192
MaeIII GTNAC 1 cut(s) 188
MalI GATC 1 cut(s) 268
MboI GATC 1 cut(s) 266
MboII GAAGA 5 cut(s) 31, 233, 238, 276, 362
MflI RGATCY 1 cut(s) 266
MluCI AATT 1 cut(s) 351
MlyI GAGTC 2 cut(s) 16, 159
MroNI GCCGGC 1 cut(s) 55
MseI TTAA 3 cut(s) 210, 339, 429
MspA1I CMGCKG 1 cut(s) 425
MspI CCGG 2 cut(s) 56, 162
MspR9I CCNGG 1 cut(s) 65
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 2 cut(s) 42, 76
MwoI GCNNNNNNNGC 6 cut(s) 65, 126, 225, 410, 419, 503
NaeI GCCGGC 1 cut(s) 57
NdeII GATC 1 cut(s) 266
NgoMIV GCCGGC 1 cut(s) 55
NlaIII CATG 2 cut(s) 199, 400
NlaIV GGNNCC 5 cut(s) 37, 38, 130, 160, 268
NmuCI GTSAC 1 cut(s) 188
NspI RCATGY 1 cut(s) 400
PaeI GCATGC 1 cut(s) 400
PagI TCATGA 1 cut(s) 195
PceI AGGCCT 1 cut(s) 479
PcsI WCGNNNNNNNCGW 5 cut(s) 105, 132, 141, 150, 353
PdiI GCCGGC 1 cut(s) 57
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 6 cut(s) 61, 75, 395, 424, 470, 499
PleI GAGTC 2 cut(s) 15, 159
PpsI GAGTC 2 cut(s) 15, 159
PpuMI RGGWCCY 2 cut(s) 36, 328
Psp5II RGGWCCY 2 cut(s) 36, 328
Psp6I CCWGG 1 cut(s) 63
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 5 cut(s) 37, 38, 130, 160, 268
PspPI GGNCC 4 cut(s) 36, 46, 61, 328
PspPPI RGGWCCY 2 cut(s) 36, 328
PstI CTGCAG 1 cut(s) 395
PsuI RGATCY 1 cut(s) 266
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 425
SalI GTCGAC 1 cut(s) 98
SaqAI TTAA 3 cut(s) 210, 339, 429
SatI GCNGC 6 cut(s) 60, 74, 394, 423, 469, 498
Sau3AI GATC 1 cut(s) 266
Sau96I GGNCC 4 cut(s) 36, 46, 61, 328
SchI GAGTC 2 cut(s) 16, 159
ScrFI CCNGG 1 cut(s) 65
SfaNI GCATC 2 cut(s) 42, 400
SfcI CTRYAG 1 cut(s) 391
SinI GGWCC 2 cut(s) 36, 328
SphI GCATGC 1 cut(s) 400
Sse9I AATT 1 cut(s) 351
SseBI AGGCCT 1 cut(s) 479
SsiI CCGC 7 cut(s) 40, 59, 74, 118, 180, 186, 270
StuI AGGCCT 1 cut(s) 479
StyD4I CCNGG 1 cut(s) 63
StyI CCWWGG 1 cut(s) 31
TaiI ACGT 2 cut(s) 126, 195
TaqI TCGA 3 cut(s) 17, 99, 135
TasI AATT 1 cut(s) 351
TauI GCSGC 2 cut(s) 62, 76
Tru1I TTAA 3 cut(s) 210, 339, 429
Tru9I TTAA 3 cut(s) 210, 339, 429
TscAI CASTG 1 cut(s) 395
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 4 cut(s) 393, 422, 468, 497
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 212
TspGWI ACGGA 3 cut(s) 142, 277, 362
TspRI CASTG 1 cut(s) 395
Tth111I GACNNNGTC 1 cut(s) 242
VpaK11BI GGWCC 2 cut(s) 36, 328
XceI RCATGY 1 cut(s) 400
XmiI GTMKAC 1 cut(s) 99
ZraI GACGTC 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.