AT4G22758

At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011189
Physical Location & Seq
Forward (+)
11958128 .. 11960071
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22758.2

Sequence Viewer

Length: 768 bp
ATGTCTGATTCGATTCACCGGAGAAAAGTTTCGAATCCTGTAGGAAATGGAGGTCGGAGCTCCAGAACAAGACGAACAGCTTTCAGATATGTTTCCGACAAGAATAATCGCTCTAAATCGAGTAATAAGGTTTTCGAGAGAAGCTTCTCAGAGCCGAGTCTTAATCGCCACCGCGACGGACAGAGTAATCACTTGCGGCGACCATCGCCGATGAGAGGTTTACCGATGGAAGAAGAAACAAAACCGATCGTTTACTTGCCTAGGATTCGCTCTGAGGTTTTCGCTTCGTCTCCGTCGTTGTTGAACTTATATTCGCCCTCGTCTTCATCTCCGATCAATCAAGAGGGTAATACAAAAGAAGCACCAAAAGTAATAATCAGTGTAGCAGTAGAAGGAAGTCCTGGACCGGTAAGAGCAATGGTTAAACTGAGTTGCAACGTCGAAGAAACGATCAAAATTGTCGTTGACAAGTACTGTAAAGAAGGAAGAACTCCAAAACTCGATCGAGATTCTGCCTTTGAATTGCATCAGTCACATTTCAGCATCCAGTGTTTGGAGAAAAGAGAAATAATTGGAGAATTAGGCAGTAGAAGCTTCTACATGAGAAAGAAAGCTCCTGAAACCGGAGGTTCGTTCGCCGGGATTTCTCCAGCGAGAACGAGCCTAATTCCGTCGTCGAATTTGATCGGATCGTGCATCGCTCAGTTGATTGGGAAAATCATGAGGAGAACAAGAAGGATATGGAACATATTGGTATGTGTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

28.54

Weight (kDa)

10.28

Isoelectric Point (pI)

73.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 122 - 184 3.1e-21 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 553
AccII CGCG 1 cut(s) 174
AciI CCGC 2 cut(s) 172, 196
AclWI GGATC 1 cut(s) 697
AcsI RAATTY 1 cut(s) 679
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 3 cut(s) 215, 553, 623
AgeI ACCGGT 1 cut(s) 406
AgsI TTSAA 2 cut(s) 304, 521
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 5 cut(s) 60, 80, 144, 594, 614
AluI AGCT 5 cut(s) 60, 80, 144, 594, 614
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 294
AlwI GGATC 1 cut(s) 697
ApoI RAATTY 1 cut(s) 679
AsiGI ACCGGT 1 cut(s) 406
Asp700I GAANNNNTTC 1 cut(s) 28
AspA2I CCTAGG 1 cut(s) 260
AspS9I GGNCC 1 cut(s) 404
AsuC2I CCSGG 1 cut(s) 640
AsuHPI GGTGA 1 cut(s) 8
AsuII TTCGAA 1 cut(s) 32
AvaII GGWCC 1 cut(s) 404
AvrII CCTAGG 1 cut(s) 260
BanII GRGCYC 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 62
BccI CCATC 2 cut(s) 211, 220
BciT130I CCWGG 1 cut(s) 402
BcnI CCSGG 1 cut(s) 640
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 1 cut(s) 261
BfmI CTRYAG 1 cut(s) 39
BisI GCNGC 1 cut(s) 197
BlnI CCTAGG 1 cut(s) 260
BlsI GCNGC 1 cut(s) 198
BmcAI AGTACT 1 cut(s) 473
Bme1390I CCNGG 2 cut(s) 402, 640
Bme18I GGWCC 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 404
BmrFI CCNGG 2 cut(s) 402, 640
BmsI GCATC 3 cut(s) 535, 552, 705
BpiI GAAGAC 1 cut(s) 315
BpmI CTGGAG 2 cut(s) 46, 633
Bpu14I TTCGAA 1 cut(s) 32
BpuMI CCSGG 1 cut(s) 640
BsaJI CCNNGG 1 cut(s) 260
BsaWI WCCGGW 3 cut(s) 18, 406, 623
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 553, 623
Bse118I RCCGGY 1 cut(s) 406
Bse1I ACTGG 1 cut(s) 547
Bse3DI GCAATG 1 cut(s) 423
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 260
BseGI GGATG 1 cut(s) 543
BseLI CCNNNNNNNGG 3 cut(s) 215, 553, 623
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 4 cut(s) 162, 264, 419, 716
BseNI ACTGG 1 cut(s) 547
BseRI GAGGAG 1 cut(s) 739
Bsh1236I CGCG 1 cut(s) 174
Bsh1285I CGRYCG 2 cut(s) 249, 505
BshTI ACCGGT 1 cut(s) 406
BsiEI CGRYCG 2 cut(s) 249, 505
BsiHKAI GWGCWC 1 cut(s) 62
BsiSI CCGG 4 cut(s) 19, 407, 624, 639
BslI CCNNNNNNNGG 3 cut(s) 215, 553, 623
BsmAI GTCTC 1 cut(s) 294
BsmBI CGTCTC 1 cut(s) 294
Bsp119I TTCGAA 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 62
Bsp143I GATC 6 cut(s) 246, 333, 450, 502, 684, 689
BspACI CCGC 2 cut(s) 172, 196
BspCNI CTCAG 4 cut(s) 161, 265, 420, 715
BspFNI CGCG 1 cut(s) 174
BspHI TCATGA 1 cut(s) 720
BspPI GGATC 1 cut(s) 697
BspT104I TTCGAA 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 406
BsrI ACTGG 1 cut(s) 547
BssAI RCCGGY 1 cut(s) 406
BssECI CCNNGG 1 cut(s) 260
BssMI GATC 6 cut(s) 246, 333, 450, 502, 684, 689
BssT1I CCWWGG 1 cut(s) 260
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 1 cut(s) 476
BstBI TTCGAA 1 cut(s) 32
BstDEI CTNAG 4 cut(s) 148, 273, 428, 702
BstF5I GGATG 1 cut(s) 543
BstFNI CGCG 1 cut(s) 174
BstKTI GATC 6 cut(s) 249, 336, 453, 505, 687, 692
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 6 cut(s) 246, 333, 450, 502, 684, 689
BstMCI CGRYCG 2 cut(s) 249, 505
BstMWI GCNNNNNNNGC 2 cut(s) 205, 591
BstNI CCWGG 1 cut(s) 402
BstSCI CCNGG 2 cut(s) 400, 638
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 1 cut(s) 174
BstV2I GAAGAC 1 cut(s) 315
BtgZI GCGATG 2 cut(s) 189, 682
BtsCI GGATG 1 cut(s) 543
BtsIMutI CAGTG 2 cut(s) 385, 554
CciI TCATGA 1 cut(s) 720
Cfr10I RCCGGY 1 cut(s) 406
Cfr13I GGNCC 1 cut(s) 404
Csp6I GTAC 1 cut(s) 472
CspAI ACCGGT 1 cut(s) 406
CviAII CATG 2 cut(s) 601, 721
CviJI RGCY 7 cut(s) 60, 80, 144, 154, 594, 614, 663
CviKI_1 RGCY 7 cut(s) 60, 80, 144, 154, 594, 614, 663
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 4 cut(s) 148, 273, 428, 702
DpnI GATC 6 cut(s) 248, 335, 452, 504, 686, 691
DpnII GATC 6 cut(s) 246, 333, 450, 502, 684, 689
Ecl136II GAGCTC 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 260
Eco24I GRGCYC 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 404
Eco53kI GAGCTC 1 cut(s) 60
EcoICRI GAGCTC 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 260
EcoT38I GRGCYC 1 cut(s) 62
ErhI CCWWGG 1 cut(s) 260
Esp3I CGTCTC 1 cut(s) 294
FaeI CATG 2 cut(s) 604, 724
FaiI YATR 7 cut(s) 90, 310, 602, 722, 742, 749, 757
FatI CATG 2 cut(s) 600, 720
Fnu4HI GCNGC 1 cut(s) 197
FokI GGATG 1 cut(s) 530
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 1 cut(s) 197
FspBI CTAG 1 cut(s) 261
GluI GCNGC 1 cut(s) 197
GsuI CTGGAG 2 cut(s) 46, 633
HapII CCGG 4 cut(s) 19, 407, 624, 639
Hin1II CATG 2 cut(s) 604, 724
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HindIII AAGCTT 2 cut(s) 142, 592
HinfI GANTC 6 cut(s) 8, 13, 34, 157, 265, 509
HpaII CCGG 4 cut(s) 19, 407, 624, 639
HphI GGTGA 1 cut(s) 8
Hpy166II GTNNAC 3 cut(s) 221, 253, 466
Hpy188I TCNGA 8 cut(s) 7, 57, 86, 97, 151, 274, 333, 689
Hpy188III TCNNGA 6 cut(s) 63, 136, 341, 506, 617, 721
Hpy8I GTNNAC 3 cut(s) 221, 253, 466
Hpy99I CGWCG 5 cut(s) 179, 298, 443, 676, 679
HpyAV CCTTC 3 cut(s) 386, 476, 729
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 4 cut(s) 435, 526, 696, 763
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 591
HpyF3I CTNAG 4 cut(s) 148, 273, 428, 702
HpySE526I ACGT 1 cut(s) 438
Hsp92II CATG 2 cut(s) 604, 724
Kzo9I GATC 6 cut(s) 246, 333, 450, 502, 684, 689
LmnI GCTCC 3 cut(s) 57, 65, 619
LweI GCATC 3 cut(s) 535, 552, 705
MaeI CTAG 1 cut(s) 261
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 1 cut(s) 531
MalI GATC 6 cut(s) 248, 335, 452, 504, 686, 691
MboI GATC 6 cut(s) 246, 333, 450, 502, 684, 689
MboII GAAGA 5 cut(s) 242, 245, 315, 455, 498
MhlI GDGCHC 1 cut(s) 62
MluCI AATT 6 cut(s) 456, 521, 570, 578, 666, 679
MlyI GAGTC 1 cut(s) 166
MmeI TCCRAC 2 cut(s) 35, 120
MnlI CCTC 7 cut(s) 44, 209, 268, 328, 337, 620, 717
MroXI GAANNNNTTC 1 cut(s) 28
MseI TTAA 2 cut(s) 162, 423
MspI CCGG 4 cut(s) 19, 407, 624, 639
MspR9I CCNGG 2 cut(s) 402, 640
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 205, 591
NciI CCSGG 1 cut(s) 640
NdeII GATC 6 cut(s) 246, 333, 450, 502, 684, 689
NlaIII CATG 2 cut(s) 604, 724
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 1 cut(s) 531
NspV TTCGAA 1 cut(s) 32
PagI TCATGA 1 cut(s) 720
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PdmI GAANNNNTTC 1 cut(s) 28
PfeI GAWTC 5 cut(s) 8, 13, 34, 265, 509
PflMI CCANNNNNTGG 1 cut(s) 553
PfoI TCCNGGA 1 cut(s) 400
PinAI ACCGGT 1 cut(s) 406
PkrI GCNGC 1 cut(s) 198
Ple19I CGATCG 2 cut(s) 249, 505
PleI GAGTC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PspPI GGNCC 1 cut(s) 404
PvuI CGATCG 2 cut(s) 249, 505
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
SacI GAGCTC 1 cut(s) 62
SaqAI TTAA 2 cut(s) 162, 423
SatI GCNGC 1 cut(s) 197
Sau3AI GATC 6 cut(s) 246, 333, 450, 502, 684, 689
Sau96I GGNCC 1 cut(s) 404
ScaI AGTACT 1 cut(s) 473
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 2 cut(s) 402, 640
SduI GDGCHC 1 cut(s) 62
SfaNI GCATC 3 cut(s) 535, 552, 705
SfcI CTRYAG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 32
SinI GGWCC 1 cut(s) 404
Sse9I AATT 6 cut(s) 456, 521, 570, 578, 666, 679
SsiI CCGC 2 cut(s) 172, 196
SspMI CTAG 1 cut(s) 261
SstI GAGCTC 1 cut(s) 62
StyD4I CCNGG 2 cut(s) 400, 638
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 1 cut(s) 476
TaiI ACGT 1 cut(s) 441
TaqI TCGA 8 cut(s) 11, 32, 119, 135, 441, 501, 505, 677
TasI AATT 6 cut(s) 456, 521, 570, 578, 666, 679
TatI WGTACW 1 cut(s) 471
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 5 cut(s) 8, 13, 34, 265, 509
Tru1I TTAA 2 cut(s) 162, 423
Tru9I TTAA 2 cut(s) 162, 423
TscAI CASTG 2 cut(s) 385, 554
TseFI GTSAC 1 cut(s) 531
Tsp45I GTSAC 1 cut(s) 531
TspDTI ATGAA 1 cut(s) 315
TspGWI ACGGA 3 cut(s) 192, 282, 660
TspRI CASTG 2 cut(s) 385, 554
Van91I CCANNNNNTGG 1 cut(s) 553
VpaK11BI GGWCC 1 cut(s) 404
XapI RAATTY 1 cut(s) 679
XmaJI CCTAGG 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 28
XspI CTAG 1 cut(s) 261
ZrmI AGTACT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.