Rroxscaffold_3G00255380

At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011189
Physical Location & Seq
Forward (+)
49624849 .. 49627279
2431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00255380.1

Sequence Viewer

Length: 714 bp
ATGTCAGAGAGGGGTCTCCGGCGACGATCTCAGGTGACAGGTAGTGGCCGGAGGACCCGGCTTTCGCACCCTTCACCATCGTCGCACCGGAAGACCCCACCAGCCCGGCGATCGCCCAAGCAACGTTCCAAGCCTATAAAGATTCTCAAGCGATGCTCCTCCGAGCCTCTGCTCCGGAGCCCTGTTGCCGATTTCGGCGAGGAGGTGGTGCCGCATGTTGGTTGGGAGTTGAGGTCGGAGCCGGAGGGAGTTCTCTTCCGGCCTCAAACTTGTGTCGACGTTTTTGCGTCGTCTCCGACGTTGATGGCGCCCTTCTCTCCCAATAATCATGAGGCATACAAGAAAGATGCAAAGGTGGTCTTGAATGTTTCGGTTGAAGGAAGTCCAGGACCGGTGAGGGCCATGGTGAAGTTGGGATCTACAGTGGAAGAGACTATCAAGCTTGTTGTAGACAAATATAGTAAAGAAGGGAGAACTCCCAAGCTTCTTAAGGACGAGGCATCGTCCTTTGAGTTACACCAGTCCTACTTCAGCCTTCAAAGTTTGGATAAATCAGACTTAATTGGGGATGTTGGTAGCAGAAGCTTCTATCTTCGAAAAAGCAGCAGCAACAATAGAGGATCAAGTACTTCTTTGGAAGTCATTCCTGTGAGAGCAAACTCTCCTCCACCTGCTCCGTCCTGGATTGTTACTTCCAAATTTTATTGCTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

237

Amino Acids

26.23

Weight (kDa)

10.0

Isoelectric Point (pI)

79.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 115 - 199 3.8e-34 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 679
Acc36I ACCTGC 1 cut(s) 679
AccB1I GGYRCC 2 cut(s) 208, 307
AccI GTMKAC 2 cut(s) 276, 450
AccIII TCCGGA 1 cut(s) 174
AciI CCGC 1 cut(s) 212
AclI AACGTT 1 cut(s) 124
AclWI GGATC 2 cut(s) 424, 628
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 1 cut(s) 698
AcuI CTGAAG 1 cut(s) 514
AcyI GRCGYC 1 cut(s) 308
AfaI GTAC 1 cut(s) 628
AfiI CCNNNNNNNGG 1 cut(s) 218
AflII CTTAAG 1 cut(s) 488
AgeI ACCGGT 1 cut(s) 391
AgsI TTSAA 3 cut(s) 364, 377, 539
AjnI CCWGG 2 cut(s) 385, 680
AluBI AGCT 3 cut(s) 442, 484, 585
AluI AGCT 3 cut(s) 442, 484, 585
Alw26I GTCTC 3 cut(s) 20, 297, 425
AlwI GGATC 2 cut(s) 424, 628
Aor13HI TCCGGA 1 cut(s) 174
AoxI GGCC 3 cut(s) 46, 260, 399
ApeKI GCWGC 2 cut(s) 603, 606
ApoI RAATTY 1 cut(s) 698
ArsI GACNNNNNNTTYG 2 cut(s) 46, 78
AsiGI ACCGGT 1 cut(s) 391
AsiSI GCGATCGC 1 cut(s) 113
Asp700I GAANNNNTTC 1 cut(s) 642
AspLEI GCGC 1 cut(s) 310
AspS9I GGNCC 3 cut(s) 54, 389, 399
AsuC2I CCSGG 2 cut(s) 58, 106
AsuHPI GGTGA 4 cut(s) 46, 66, 406, 418
AsuII TTCGAA 1 cut(s) 595
AvaII GGWCC 2 cut(s) 54, 389
BanI GGYRCC 2 cut(s) 208, 307
BanII GRGCYC 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 98
BbvI GCAGC 2 cut(s) 615, 618
BccI CCATC 2 cut(s) 85, 298
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BciT130I CCWGG 2 cut(s) 387, 682
BcnI CCSGG 2 cut(s) 58, 106
BcoDI GTCTC 3 cut(s) 20, 297, 425
BfmI CTRYAG 1 cut(s) 420
BfoI RGCGCY 1 cut(s) 311
BfrI CTTAAG 1 cut(s) 488
BfuAI ACCTGC 1 cut(s) 679
BisI GCNGC 3 cut(s) 212, 604, 607
BlsI GCNGC 3 cut(s) 213, 605, 608
BmcAI AGTACT 1 cut(s) 628
Bme1390I CCNGG 4 cut(s) 58, 106, 387, 682
Bme18I GGWCC 2 cut(s) 54, 389
BmgT120I GGNCC 3 cut(s) 54, 389, 399
BmiI GGNNCC 5 cut(s) 56, 179, 210, 240, 309
BmrFI CCNGG 4 cut(s) 58, 106, 387, 682
BmsI GCATC 3 cut(s) 143, 337, 509
BpiI GAAGAC 1 cut(s) 98
Bpu14I TTCGAA 1 cut(s) 595
BpuEI CTTGAG 1 cut(s) 131
BpuMI CCSGG 2 cut(s) 58, 106
BsaHI GRCGYC 1 cut(s) 308
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 402
BsaWI WCCGGW 3 cut(s) 87, 174, 391
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 1 cut(s) 520
BseAI TCCGGA 1 cut(s) 174
BseBI CCWGG 2 cut(s) 387, 682
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 1 cut(s) 574
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 1 cut(s) 520
BseRI GAGGAG 3 cut(s) 148, 215, 654
BseXI GCAGC 2 cut(s) 615, 618
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 3 cut(s) 48, 262, 401
BshNI GGYRCC 2 cut(s) 208, 307
BshTI ACCGGT 1 cut(s) 391
BsiEI CGRYCG 1 cut(s) 113
BsiSI CCGG 9 cut(s) 19, 49, 58, 88, 106, 175, 242, 259, 392
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 3 cut(s) 20, 297, 425
BsmBI CGTCTC 1 cut(s) 297
BsnI GGCC 3 cut(s) 48, 262, 401
Bso31I GGTCTC 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 595
Bsp1286I GDGCHC 1 cut(s) 182
Bsp13I TCCGGA 1 cut(s) 174
Bsp143I GATC 4 cut(s) 26, 110, 416, 620
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 1 cut(s) 212
BspANI GGCC 3 cut(s) 48, 262, 401
BspCNI CTCAG 1 cut(s) 43
BspEI TCCGGA 1 cut(s) 174
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 5 cut(s) 56, 179, 210, 240, 309
BspMI ACCTGC 1 cut(s) 679
BspPI GGATC 2 cut(s) 424, 628
BspT104I TTCGAA 1 cut(s) 595
BspT107I GGYRCC 2 cut(s) 208, 307
BspTI CTTAAG 1 cut(s) 488
BspTNI GGTCTC 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 391
BsrI ACTGG 1 cut(s) 520
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 4 cut(s) 26, 110, 416, 620
BssNI GRCGYC 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 2 cut(s) 387, 682
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 2 cut(s) 260, 423
BstACI GRCGYC 1 cut(s) 308
BstAFI CTTAAG 1 cut(s) 488
BstBI TTCGAA 1 cut(s) 595
BstDEI CTNAG 1 cut(s) 30
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 1 cut(s) 574
BstH2I RGCGCY 1 cut(s) 311
BstHHI GCGC 1 cut(s) 310
BstKTI GATC 4 cut(s) 29, 113, 419, 623
BstMAI GTCTC 3 cut(s) 20, 297, 425
BstMBI GATC 4 cut(s) 26, 110, 416, 620
BstMCI CGRYCG 1 cut(s) 113
BstNI CCWGG 2 cut(s) 387, 682
BstNSI RCATGY 1 cut(s) 218
BstSCI CCNGG 4 cut(s) 56, 104, 385, 680
BstSFI CTRYAG 1 cut(s) 420
BstV1I GCAGC 2 cut(s) 615, 618
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
BsuRI GGCC 3 cut(s) 48, 262, 401
BtgI CCRYGG 1 cut(s) 402
BtgZI GCGATG 1 cut(s) 166
BtsCI GGATG 1 cut(s) 574
BtsIMutI CAGTG 1 cut(s) 429
BveI ACCTGC 1 cut(s) 679
CciI TCATGA 1 cut(s) 328
CfoI GCGC 1 cut(s) 310
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 3 cut(s) 54, 389, 399
CseI GACGC 1 cut(s) 276
Csp6I GTAC 1 cut(s) 627
CspAI ACCGGT 1 cut(s) 391
CviAII CATG 3 cut(s) 215, 329, 403
CviQI GTAC 1 cut(s) 627
DdeI CTNAG 1 cut(s) 30
DinI GGCGCC 1 cut(s) 309
DpnI GATC 4 cut(s) 28, 112, 418, 622
DpnII GATC 4 cut(s) 26, 110, 416, 620
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 2 cut(s) 260, 423
EarI CTCTTC 2 cut(s) 260, 423
Eco130I CCWWGG 1 cut(s) 402
Eco24I GRGCYC 1 cut(s) 182
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 54, 389
Eco57I CTGAAG 1 cut(s) 514
EcoO109I RGGNCCY 1 cut(s) 54
EcoRII CCWGG 2 cut(s) 385, 680
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 182
EgeI GGCGCC 1 cut(s) 309
EheI GGCGCC 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 402
Esp3I CGTCTC 1 cut(s) 297
FaeI CATG 3 cut(s) 218, 332, 406
FaiI YATR 6 cut(s) 137, 216, 330, 337, 404, 459
FalI AAGNNNNNCTT 4 cut(s) 344, 376, 616, 648
FatI CATG 3 cut(s) 214, 328, 402
FblI GTMKAC 2 cut(s) 276, 450
Fnu4HI GCNGC 3 cut(s) 212, 604, 607
FokI GGATG 1 cut(s) 581
FriOI GRGCYC 1 cut(s) 182
Fsp4HI GCNGC 3 cut(s) 212, 604, 607
GlaI GCGC 1 cut(s) 309
GluI GCNGC 3 cut(s) 212, 604, 607
HaeII RGCGCY 1 cut(s) 311
HaeIII GGCC 3 cut(s) 48, 262, 401
HapII CCGG 9 cut(s) 19, 49, 58, 88, 106, 175, 242, 259, 392
HgaI GACGC 1 cut(s) 276
HhaI GCGC 1 cut(s) 310
Hin1I GRCGYC 1 cut(s) 308
Hin1II CATG 3 cut(s) 218, 332, 406
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 3 cut(s) 440, 482, 583
HinfI GANTC 1 cut(s) 142
HpaII CCGG 9 cut(s) 19, 49, 58, 88, 106, 175, 242, 259, 392
HphI GGTGA 4 cut(s) 46, 66, 406, 418
Hpy166II GTNNAC 2 cut(s) 277, 451
Hpy188I TCNGA 5 cut(s) 7, 163, 238, 297, 556
Hpy188III TCNNGA 3 cut(s) 175, 329, 361
Hpy8I GTNNAC 2 cut(s) 277, 451
Hpy99I CGWCG 5 cut(s) 27, 85, 281, 292, 301
HpyAV CCTTC 5 cut(s) 81, 322, 371, 461, 545
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 3 cut(s) 124, 279, 299
HpyCH4V TGCA 1 cut(s) 350
HpyF3I CTNAG 1 cut(s) 30
HpySE526I ACGT 3 cut(s) 124, 279, 299
Hsp92I GRCGYC 1 cut(s) 308
Hsp92II CATG 3 cut(s) 218, 332, 406
HspAI GCGC 1 cut(s) 308
KasI GGCGCC 1 cut(s) 307
Kpn2I TCCGGA 1 cut(s) 174
Kzo9I GATC 4 cut(s) 26, 110, 416, 620
LmnI GCTCC 5 cut(s) 161, 177, 177, 238, 679
Lsp1109I GCAGC 2 cut(s) 615, 618
LweI GCATC 3 cut(s) 143, 337, 509
MaeII ACGT 3 cut(s) 124, 279, 299
MaeIII GTNAC 3 cut(s) 34, 513, 688
MalI GATC 4 cut(s) 28, 112, 418, 622
MboI GATC 4 cut(s) 26, 110, 416, 620
MboII GAAGA 4 cut(s) 103, 247, 440, 584
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 1 cut(s) 182
MluCI AATT 2 cut(s) 561, 698
Mly113I GGCGCC 1 cut(s) 308
MmeI TCCRAC 2 cut(s) 216, 320
MroI TCCGGA 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 2 cut(s) 489, 560
MslI CAYNNNNRTG 1 cut(s) 647
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 9 cut(s) 19, 49, 58, 88, 106, 175, 242, 259, 392
MspR9I CCNGG 4 cut(s) 58, 106, 387, 682
MvaI CCWGG 2 cut(s) 387, 682
NarI GGCGCC 1 cut(s) 308
NciI CCSGG 2 cut(s) 58, 106
NcoI CCATGG 1 cut(s) 402
NdeII GATC 4 cut(s) 26, 110, 416, 620
NlaIII CATG 3 cut(s) 218, 332, 406
NlaIV GGNNCC 5 cut(s) 56, 179, 210, 240, 309
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 218
NspV TTCGAA 1 cut(s) 595
PagI TCATGA 1 cut(s) 328
PaqCI CACCTGC 1 cut(s) 679
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 1 cut(s) 142
PfoI TCCNGGA 2 cut(s) 385, 680
PinAI ACCGGT 1 cut(s) 391
PkrI GCNGC 3 cut(s) 213, 605, 608
Ple19I CGATCG 1 cut(s) 113
PluTI GGCGCC 1 cut(s) 311
PpuMI RGGWCCY 1 cut(s) 54
Psp1406I AACGTT 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 2 cut(s) 385, 680
PspGI CCWGG 2 cut(s) 385, 680
PspN4I GGNNCC 5 cut(s) 56, 179, 210, 240, 309
PspPI GGNCC 3 cut(s) 54, 389, 399
PspPPI RGGWCCY 1 cut(s) 54
PsuI RGATCY 1 cut(s) 416
PvuI CGATCG 1 cut(s) 113
RgaI GCGATCGC 1 cut(s) 113
RsaI GTAC 1 cut(s) 628
RsaNI GTAC 1 cut(s) 627
RseI CAYNNNNRTG 1 cut(s) 647
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 2 cut(s) 489, 560
SatI GCNGC 3 cut(s) 212, 604, 607
Sau3AI GATC 4 cut(s) 26, 110, 416, 620
Sau96I GGNCC 3 cut(s) 54, 389, 399
ScaI AGTACT 1 cut(s) 628
ScrFI CCNGG 4 cut(s) 58, 106, 387, 682
SduI GDGCHC 1 cut(s) 182
SfaAI GCGATCGC 1 cut(s) 113
SfaNI GCATC 3 cut(s) 143, 337, 509
SfcI CTRYAG 1 cut(s) 420
SfoI GGCGCC 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 595
SgfI GCGATCGC 1 cut(s) 113
SinI GGWCC 2 cut(s) 54, 389
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 2 cut(s) 146, 488
SmoI CTYRAG 2 cut(s) 146, 488
Sse9I AATT 2 cut(s) 561, 698
SsiI CCGC 1 cut(s) 212
SspDI GGCGCC 1 cut(s) 307
StyD4I CCNGG 4 cut(s) 56, 104, 385, 680
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 3 cut(s) 127, 282, 302
TaqI TCGA 2 cut(s) 276, 595
TasI AATT 2 cut(s) 561, 698
TatI WGTACW 1 cut(s) 626
TauI GCSGC 1 cut(s) 214
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 2 cut(s) 489, 560
Tru9I TTAA 2 cut(s) 489, 560
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 2 cut(s) 603, 606
Tsp45I GTSAC 1 cut(s) 34
TspGWI ACGGA 1 cut(s) 666
TspRI CASTG 1 cut(s) 429
Vha464I CTTAAG 1 cut(s) 488
VpaK11BI GGWCC 2 cut(s) 54, 389
XapI RAATTY 1 cut(s) 698
XceI RCATGY 1 cut(s) 218
XcmI CCANNNNNNNNNTGG 1 cut(s) 409
XmiI GTMKAC 2 cut(s) 276, 450
XmnI GAANNNNTTC 1 cut(s) 642
ZrmI AGTACT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.