pycom04g07000

At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011189
Physical Location & Seq
Reverse (-)
7289750 .. 7291976
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g07000.1

Sequence Viewer

Length: 807 bp
ATGTCAGAAAGGGGTCTCCGGCGACGATCTCAGGTGAGCGGAGGCCAGAAAACCAGACCGCCGAACCTATCTCCTTCGACGCACCGGAGGACCCATCAATCTCGGCGATCGCCCAAGCAGCACCACGAGCACCATTCCAAGCCTATTGAGATTCTCAAGCGATGCTCTTCCGTTCCCGTATTGGTGGGGTCAATTGGCAGCGATTTCTACGAGGAGGAGGAGAAGGAGGCTCTTCGTGTTCCGGCCATGCGGTCGGAGCCGGAGGGTGTTTTGTTCAGGCCTCACACTTGTACGGACATCTTTGCTTCCTCTCCTTCTCTGATGATGGTGCAGCCCCTGCCCTTCTCTCCCCAGAAGAACGAGGGATACAAGAAAGATGCAAAGGTGGTCTTGAATGTGTCAGTTGAAGGAAGCCCCGGACCGGTGCGCACCATGGTTAAATTAGGATCTACAGTGGAAGAAACAATTAAGCTTGTTGTAGACAAATATAGCAAAGAAGGTCGAACTCCGAAGCTTGATCACGAGGCATTGTCCTTTGAGTTGCATCAGTCTTACTTCAGCCTTCAAAGTTTGGATAAATCGGAAGTAATTGGAGATGTCGGTAGCCGAAGCTTCTATCTTAGAAAAAGCAGCAGCAGCAATGGAGGATCAACTTCTTTAATCACTGAAGTCGTTCCTGTGAGAGAAAACACTCCTCCAGCTGCTCCGCGCCCTGTATTTTTACTACCGAATTTCATTGCTCGTAAACTTGGCAAAATCGCCAGGAGGACGCATAAGCTTTGGAGAGTCATAATCTGTATCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

269

Amino Acids

30.08

Weight (kDa)

9.94

Isoelectric Point (pI)

77.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 125 - 208 1.4e-33 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 428
AccBSI CCGCTC 1 cut(s) 39
AccI GTMKAC 1 cut(s) 480
AccII CGCG 1 cut(s) 709
AciI CCGC 4 cut(s) 39, 59, 250, 707
AclWI GGATC 2 cut(s) 454, 655
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 730
AcuI CTGAAG 2 cut(s) 541, 687
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 1 cut(s) 421
AgeI ACCGGT 1 cut(s) 421
AgsI TTSAA 3 cut(s) 394, 407, 566
AjnI CCWGG 1 cut(s) 761
AluBI AGCT 5 cut(s) 472, 514, 612, 701, 778
AluI AGCT 5 cut(s) 472, 514, 612, 701, 778
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 2 cut(s) 454, 655
AlwNI CAGNNNCTG 1 cut(s) 337
AoxI GGCC 3 cut(s) 43, 243, 278
ApeKI GCWGC 7 cut(s) 118, 198, 331, 630, 633, 636, 701
ApoI RAATTY 1 cut(s) 730
AsiGI ACCGGT 1 cut(s) 421
AsiSI GCGATCGC 1 cut(s) 110
Asp700I GAANNNNTTC 1 cut(s) 672
AspLEI GCGC 2 cut(s) 429, 711
AspS9I GGNCC 2 cut(s) 90, 419
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 2 cut(s) 90, 419
BauI CACGAG 2 cut(s) 125, 521
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 7 cut(s) 130, 210, 343, 642, 645, 648, 688
BccI CCATC 2 cut(s) 102, 319
BciT130I CCWGG 1 cut(s) 763
BciVI GTATCC 1 cut(s) 359
BclI TGATCA 1 cut(s) 517
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 450
BfuI GTATCC 1 cut(s) 359
BisI GCNGC 7 cut(s) 119, 199, 332, 631, 634, 637, 702
BlsI GCNGC 7 cut(s) 120, 200, 333, 632, 635, 638, 703
Bme1390I CCNGG 2 cut(s) 417, 763
Bme18I GGWCC 2 cut(s) 90, 419
BmgT120I GGNCC 2 cut(s) 90, 419
BmiI GGNNCC 2 cut(s) 92, 258
BmrFI CCNGG 2 cut(s) 417, 763
BmsI GCATC 3 cut(s) 152, 367, 553
BpmI CTGGAG 1 cut(s) 681
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 415, 432
BsaWI WCCGGW 2 cut(s) 84, 421
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse118I RCCGGY 1 cut(s) 421
Bse3DI GCAATG 2 cut(s) 646, 735
BseBI CCWGG 1 cut(s) 763
BseDI CCNNGG 2 cut(s) 415, 432
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMI GCAATG 2 cut(s) 646, 735
BseMII CTCAG 1 cut(s) 44
BseRI GAGGAG 4 cut(s) 227, 230, 233, 684
BseXI GCAGC 7 cut(s) 130, 210, 343, 642, 645, 648, 688
BsgI GTGCAG 1 cut(s) 350
Bsh1236I CGCG 1 cut(s) 709
Bsh1285I CGRYCG 2 cut(s) 110, 254
BshFI GGCC 3 cut(s) 45, 245, 280
BshTI ACCGGT 1 cut(s) 421
BsiEI CGRYCG 2 cut(s) 110, 254
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 6 cut(s) 19, 85, 242, 260, 417, 422
BslI CCNNNNNNNGG 1 cut(s) 421
BsmAI GTCTC 1 cut(s) 20
BsnI GGCC 3 cut(s) 45, 245, 280
Bso31I GGTCTC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 5 cut(s) 26, 107, 446, 517, 647
Bsp19I CCATGG 1 cut(s) 432
BspACI CCGC 4 cut(s) 39, 59, 250, 707
BspANI GGCC 3 cut(s) 45, 245, 280
BspCNI CTCAG 1 cut(s) 43
BspFNI CGCG 1 cut(s) 709
BspLI GGNNCC 2 cut(s) 92, 258
BspPI GGATC 2 cut(s) 454, 655
BspQI GCTCTTC 2 cut(s) 172, 237
BspTNI GGTCTC 1 cut(s) 20
BsrBI CCGCTC 1 cut(s) 39
BsrDI GCAATG 2 cut(s) 646, 735
BsrFI RCCGGY 1 cut(s) 421
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 2 cut(s) 415, 432
BssMI GATC 5 cut(s) 26, 107, 446, 517, 647
BssSI CACGAG 2 cut(s) 125, 521
BssT1I CCWWGG 1 cut(s) 432
Bst2BI CACGAG 2 cut(s) 125, 521
Bst2UI CCWGG 1 cut(s) 763
Bst4CI ACNGT 1 cut(s) 454
Bst6I CTCTTC 2 cut(s) 172, 237
BstAPI GCANNNNNTGC 1 cut(s) 337
BstDEI CTNAG 2 cut(s) 30, 620
BstDSI CCRYGG 1 cut(s) 432
BstFNI CGCG 1 cut(s) 709
BstHHI GCGC 2 cut(s) 429, 711
BstKTI GATC 5 cut(s) 29, 110, 449, 520, 650
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 5 cut(s) 26, 107, 446, 517, 647
BstMCI CGRYCG 2 cut(s) 110, 254
BstMWI GCNNNNNNNGC 5 cut(s) 118, 127, 256, 337, 636
BstNI CCWGG 1 cut(s) 763
BstSCI CCNGG 2 cut(s) 415, 761
BstSFI CTRYAG 1 cut(s) 450
BstUI CGCG 1 cut(s) 709
BstV1I GCAGC 7 cut(s) 130, 210, 343, 642, 645, 648, 688
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 3 cut(s) 45, 245, 280
BtgI CCRYGG 1 cut(s) 432
BtgZI GCGATG 1 cut(s) 175
BtsIMutI CAGTG 2 cut(s) 459, 663
CaiI CAGNNNCTG 1 cut(s) 337
CfoI GCGC 2 cut(s) 429, 711
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 2 cut(s) 90, 419
CpoI CGGWCCG 1 cut(s) 419
CseI GACGC 2 cut(s) 88, 778
Csp6I GTAC 1 cut(s) 291
CspAI ACCGGT 1 cut(s) 421
CspI CGGWCCG 1 cut(s) 419
CviAII CATG 2 cut(s) 247, 433
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 2 cut(s) 30, 620
DpnI GATC 5 cut(s) 28, 109, 448, 519, 649
DpnII GATC 5 cut(s) 26, 107, 446, 517, 647
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 2 cut(s) 172, 237
EarI CTCTTC 2 cut(s) 172, 237
Eco130I CCWWGG 1 cut(s) 432
Eco147I AGGCCT 1 cut(s) 280
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 90, 419
Eco57I CTGAAG 2 cut(s) 541, 687
EcoO109I RGGNCCY 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 761
EcoT14I CCWWGG 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 2 cut(s) 250, 436
FaiI YATR 5 cut(s) 248, 434, 489, 774, 791
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FatI CATG 2 cut(s) 246, 432
FbaI TGATCA 1 cut(s) 517
FblI GTMKAC 1 cut(s) 480
Fnu4HI GCNGC 7 cut(s) 119, 199, 332, 631, 634, 637, 702
Fsp4HI GCNGC 7 cut(s) 119, 199, 332, 631, 634, 637, 702
FspAI RTGCGCAY 1 cut(s) 428
FspI TGCGCA 1 cut(s) 428
GlaI GCGC 2 cut(s) 428, 710
GluI GCNGC 7 cut(s) 119, 199, 332, 631, 634, 637, 702
GsuI CTGGAG 1 cut(s) 681
HaeIII GGCC 3 cut(s) 45, 245, 280
HapII CCGG 6 cut(s) 19, 85, 242, 260, 417, 422
HgaI GACGC 2 cut(s) 88, 778
HhaI GCGC 2 cut(s) 429, 711
Hin1II CATG 2 cut(s) 250, 436
Hin6I GCGC 2 cut(s) 427, 709
HinP1I GCGC 2 cut(s) 427, 709
HindIII AAGCTT 4 cut(s) 470, 512, 610, 776
HinfI GANTC 2 cut(s) 151, 786
HpaII CCGG 6 cut(s) 19, 85, 242, 260, 417, 422
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 2 cut(s) 481, 746
Hpy188I TCNGA 5 cut(s) 7, 256, 321, 510, 583
Hpy188III TCNNGA 2 cut(s) 391, 521
Hpy8I GTNNAC 2 cut(s) 481, 746
Hpy99I CGWCG 2 cut(s) 27, 82
HpyAV CCTTC 7 cut(s) 84, 217, 324, 352, 401, 491, 572
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4V TGCA 3 cut(s) 331, 380, 544
HpyF10VI GCNNNNNNNGC 5 cut(s) 118, 127, 256, 337, 636
HpyF3I CTNAG 2 cut(s) 30, 620
Hsp92II CATG 2 cut(s) 250, 436
HspAI GCGC 2 cut(s) 427, 709
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 5 cut(s) 26, 107, 446, 517, 647
LguI GCTCTTC 2 cut(s) 172, 237
LmnI GCTCC 2 cut(s) 256, 709
Lsp1109I GCAGC 7 cut(s) 130, 210, 343, 642, 645, 648, 688
LweI GCATC 3 cut(s) 152, 367, 553
MalI GATC 5 cut(s) 28, 109, 448, 519, 649
MbiI CCGCTC 1 cut(s) 39
MboI GATC 5 cut(s) 26, 107, 446, 517, 647
MboII GAAGA 4 cut(s) 159, 224, 367, 470
MfeI CAATTG 1 cut(s) 192
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 5 cut(s) 192, 440, 465, 588, 730
MlyI GAGTC 1 cut(s) 795
MmeI TCCRAC 1 cut(s) 234
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 3 cut(s) 438, 468, 659
MspA1I CMGCKG 1 cut(s) 701
MspI CCGG 6 cut(s) 19, 85, 242, 260, 417, 422
MspR9I CCNGG 2 cut(s) 417, 763
MunI CAATTG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 763
MvnI CGCG 1 cut(s) 709
MwoI GCNNNNNNNGC 5 cut(s) 118, 127, 256, 337, 636
NciI CCSGG 1 cut(s) 417
NcoI CCATGG 1 cut(s) 432
NdeII GATC 5 cut(s) 26, 107, 446, 517, 647
NlaIII CATG 2 cut(s) 250, 436
NlaIV GGNNCC 2 cut(s) 92, 258
NmeAIII GCCGAG 1 cut(s) 82
NsbI TGCGCA 1 cut(s) 428
PceI AGGCCT 1 cut(s) 280
PciSI GCTCTTC 2 cut(s) 172, 237
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 1 cut(s) 151
PinAI ACCGGT 1 cut(s) 421
PkrI GCNGC 7 cut(s) 120, 200, 333, 632, 635, 638, 703
Ple19I CGATCG 1 cut(s) 110
PleI GAGTC 1 cut(s) 794
PpsI GAGTC 1 cut(s) 794
PpuMI RGGWCCY 1 cut(s) 90
Psp5II RGGWCCY 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 761
PspGI CCWGG 1 cut(s) 761
PspN4I GGNNCC 2 cut(s) 92, 258
PspPI GGNCC 2 cut(s) 90, 419
PspPPI RGGWCCY 1 cut(s) 90
PsrI GAACNNNNNNTAC 2 cut(s) 350, 382
PstNI CAGNNNCTG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 446
PvuI CGATCG 1 cut(s) 110
PvuII CAGCTG 1 cut(s) 701
RgaI GCGATCGC 1 cut(s) 110
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
Rsr2I CGGWCCG 1 cut(s) 419
RsrII CGGWCCG 1 cut(s) 419
SapI GCTCTTC 2 cut(s) 172, 237
SaqAI TTAA 3 cut(s) 438, 468, 659
SatI GCNGC 7 cut(s) 119, 199, 332, 631, 634, 637, 702
Sau3AI GATC 5 cut(s) 26, 107, 446, 517, 647
Sau96I GGNCC 2 cut(s) 90, 419
SchI GAGTC 1 cut(s) 795
ScrFI CCNGG 2 cut(s) 417, 763
SduI GDGCHC 1 cut(s) 132
SetI ASST 9 cut(s) 36, 69, 387, 474, 502, 516, 614, 703, 780
SfaAI GCGATCGC 1 cut(s) 110
SfaNI GCATC 3 cut(s) 152, 367, 553
SfcI CTRYAG 1 cut(s) 450
SgfI GCGATCGC 1 cut(s) 110
SinI GGWCC 2 cut(s) 90, 419
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 5 cut(s) 192, 440, 465, 588, 730
SseBI AGGCCT 1 cut(s) 280
SsiI CCGC 4 cut(s) 39, 59, 250, 707
StuI AGGCCT 1 cut(s) 280
StyD4I CCNGG 2 cut(s) 415, 761
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 1 cut(s) 454
TaqI TCGA 2 cut(s) 77, 502
TasI AATT 5 cut(s) 192, 440, 465, 588, 730
TfiI GAWTC 1 cut(s) 151
Tru1I TTAA 3 cut(s) 438, 468, 659
Tru9I TTAA 3 cut(s) 438, 468, 659
TscAI CASTG 2 cut(s) 459, 670
TseI GCWGC 7 cut(s) 118, 198, 331, 630, 633, 636, 701
TspDTI ATGAA 1 cut(s) 724
TspGWI ACGGA 2 cut(s) 160, 308
TspRI CASTG 2 cut(s) 459, 670
VpaK11BI GGWCC 2 cut(s) 90, 419
XapI RAATTY 1 cut(s) 730
XmiI GTMKAC 1 cut(s) 480
XmnI GAANNNNTTC 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.