Rh7DG194600

At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0011189
Physical Location & Seq
Reverse (-)
18251662 .. 18254791
3130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG194600.1

Sequence Viewer

Length: 639 bp
ATGTCAGAGAGGGGTCTCCGGCGACGATCTCAGGTGACGGGTAGTGGCCGGAGGACCCGGCTTTCGAACGCTTCACCATCGTCGCACCGGAAGACCCCACCAGCTCGGCGATCGCCCAAGCAACGTTCCAAGCCTATAAAGATTCTCAAGCGATGCTCCTCCGAGCCCCTGCTCCGGAGCCCTGTTGCCGATTTCGGCGAGGAGGTGGTGCCGCATGTTGGTTGGGAGCTGAGGTCGGAGCCGGAGGGAGTTCTCTTCCGGCCTCAAACTTGTGTCGACGTTTTTGCGTCGTCTCCGACGTTGATGACGCCCTTCTCTCCCAATAATCATGAGGCATACAAGAAAGATGCAAAGGTGGTCTTGAATGTTTCGGTTGAAGGAAGTCCAGGACCGGTAAGGGCCATGGTGAAGTTGGGATCTACAGTTGAAGAGACTATCAAGCTTGTTGTAGACAAATATACTAAAGAAGGCAGAACTCCCAAGCTTCTCAAGGATGAGGCATCGTCCTTTGAGTTACACCAGTCCTACTTCAGCCTTCAAAGTAAGTCAAAATCATATAGTTATTCATGTTCTATTAACTTTTTTCTTTTTCTTGGAGTTGCTCTGCCAGATTGTAGAATTAATAGTTTGATTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

23.64

Weight (kDa)

9.9

Isoelectric Point (pI)

70.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 115 - 196 4.6e-26 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 208
AccI GTMKAC 2 cut(s) 276, 450
AccIII TCCGGA 1 cut(s) 174
AciI CCGC 1 cut(s) 212
AclI AACGTT 1 cut(s) 124
AclWI GGATC 1 cut(s) 424
AcoI YGGCCR 1 cut(s) 46
AcuI CTGAAG 1 cut(s) 514
AcyI GRCGYC 1 cut(s) 308
AfiI CCNNNNNNNGG 2 cut(s) 174, 218
AgeI ACCGGT 1 cut(s) 391
AgsI TTSAA 4 cut(s) 364, 377, 428, 539
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 4 cut(s) 104, 229, 442, 484
AluI AGCT 4 cut(s) 104, 229, 442, 484
Alw26I GTCTC 3 cut(s) 20, 297, 425
AlwI GGATC 1 cut(s) 424
Aor13HI TCCGGA 1 cut(s) 174
AoxI GGCC 3 cut(s) 46, 260, 399
ArsI GACNNNNNNTTYG 2 cut(s) 46, 78
AseI ATTAAT 1 cut(s) 621
AsiGI ACCGGT 1 cut(s) 391
AsiSI GCGATCGC 1 cut(s) 113
AspS9I GGNCC 3 cut(s) 54, 389, 399
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 3 cut(s) 46, 66, 418
AsuII TTCGAA 1 cut(s) 65
AvaII GGWCC 2 cut(s) 54, 389
BanI GGYRCC 1 cut(s) 208
BanII GRGCYC 2 cut(s) 168, 182
BbsI GAAGAC 1 cut(s) 98
BbvCI CCTCAGC 1 cut(s) 230
BccI CCATC 1 cut(s) 85
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BciT130I CCWGG 1 cut(s) 387
BcnI CCSGG 1 cut(s) 58
BcoDI GTCTC 3 cut(s) 20, 297, 425
BfmI CTRYAG 1 cut(s) 420
BisI GCNGC 1 cut(s) 212
BlsI GCNGC 1 cut(s) 213
Bme1390I CCNGG 2 cut(s) 58, 387
Bme18I GGWCC 2 cut(s) 54, 389
BmgT120I GGNCC 3 cut(s) 54, 389, 399
BmiI GGNNCC 4 cut(s) 56, 179, 210, 240
BmrFI CCNGG 2 cut(s) 58, 387
BmsI GCATC 3 cut(s) 143, 337, 509
BpiI GAAGAC 1 cut(s) 98
Bpu10I CCTNAGC 1 cut(s) 230
Bpu14I TTCGAA 1 cut(s) 65
BpuEI CTTGAG 2 cut(s) 131, 473
BpuMI CCSGG 1 cut(s) 58
BsaHI GRCGYC 1 cut(s) 308
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 402
BsaWI WCCGGW 3 cut(s) 87, 174, 391
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 218
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 1 cut(s) 520
BseAI TCCGGA 1 cut(s) 174
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 1 cut(s) 499
BseLI CCNNNNNNNGG 2 cut(s) 174, 218
BseMII CTCAG 2 cut(s) 44, 221
BseNI ACTGG 1 cut(s) 520
BseRI GAGGAG 2 cut(s) 148, 215
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 3 cut(s) 48, 262, 401
BshNI GGYRCC 1 cut(s) 208
BshTI ACCGGT 1 cut(s) 391
BsiEI CGRYCG 1 cut(s) 113
BsiSI CCGG 8 cut(s) 19, 49, 58, 88, 175, 242, 259, 392
BslI CCNNNNNNNGG 2 cut(s) 174, 218
BsmAI GTCTC 3 cut(s) 20, 297, 425
BsmBI CGTCTC 1 cut(s) 297
BsnI GGCC 3 cut(s) 48, 262, 401
Bso31I GGTCTC 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 65
Bsp1286I GDGCHC 2 cut(s) 168, 182
Bsp13I TCCGGA 1 cut(s) 174
Bsp143I GATC 3 cut(s) 26, 110, 416
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 1 cut(s) 212
BspANI GGCC 3 cut(s) 48, 262, 401
BspCNI CTCAG 2 cut(s) 43, 222
BspEI TCCGGA 1 cut(s) 174
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 4 cut(s) 56, 179, 210, 240
BspPI GGATC 1 cut(s) 424
BspT104I TTCGAA 1 cut(s) 65
BspT107I GGYRCC 1 cut(s) 208
BspTNI GGTCTC 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 391
BsrI ACTGG 1 cut(s) 520
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 3 cut(s) 26, 110, 416
BssNI GRCGYC 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 2 cut(s) 260, 423
BstACI GRCGYC 1 cut(s) 308
BstBI TTCGAA 1 cut(s) 65
BstDEI CTNAG 2 cut(s) 30, 230
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 1 cut(s) 499
BstKTI GATC 3 cut(s) 29, 113, 419
BstMAI GTCTC 3 cut(s) 20, 297, 425
BstMBI GATC 3 cut(s) 26, 110, 416
BstMCI CGRYCG 1 cut(s) 113
BstNI CCWGG 1 cut(s) 387
BstNSI RCATGY 1 cut(s) 218
BstSCI CCNGG 2 cut(s) 56, 385
BstSFI CTRYAG 1 cut(s) 420
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
BsuRI GGCC 3 cut(s) 48, 262, 401
BtgI CCRYGG 1 cut(s) 402
BtgZI GCGATG 1 cut(s) 166
BtsCI GGATG 1 cut(s) 499
CciI TCATGA 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 3 cut(s) 54, 389, 399
CseI GACGC 2 cut(s) 276, 316
CspAI ACCGGT 1 cut(s) 391
CviAII CATG 4 cut(s) 215, 329, 403, 567
DdeI CTNAG 2 cut(s) 30, 230
DpnI GATC 3 cut(s) 28, 112, 418
DpnII GATC 3 cut(s) 26, 110, 416
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 2 cut(s) 260, 423
EarI CTCTTC 2 cut(s) 260, 423
Eco130I CCWWGG 1 cut(s) 402
Eco24I GRGCYC 2 cut(s) 168, 182
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 54, 389
Eco57I CTGAAG 1 cut(s) 514
EcoO109I RGGNCCY 1 cut(s) 54
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 2 cut(s) 168, 182
ErhI CCWWGG 1 cut(s) 402
Esp3I CGTCTC 1 cut(s) 297
FaeI CATG 4 cut(s) 218, 332, 406, 570
FaiI YATR 9 cut(s) 137, 216, 330, 337, 404, 459, 556, 558, 568
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FatI CATG 4 cut(s) 214, 328, 402, 566
FblI GTMKAC 2 cut(s) 276, 450
Fnu4HI GCNGC 1 cut(s) 212
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 2 cut(s) 168, 182
Fsp4HI GCNGC 1 cut(s) 212
GluI GCNGC 1 cut(s) 212
HaeIII GGCC 3 cut(s) 48, 262, 401
HapII CCGG 8 cut(s) 19, 49, 58, 88, 175, 242, 259, 392
HgaI GACGC 2 cut(s) 276, 316
Hin1I GRCGYC 1 cut(s) 308
Hin1II CATG 4 cut(s) 218, 332, 406, 570
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 2 cut(s) 440, 482
HinfI GANTC 1 cut(s) 142
HpaII CCGG 8 cut(s) 19, 49, 58, 88, 175, 242, 259, 392
HphI GGTGA 3 cut(s) 46, 66, 418
Hpy166II GTNNAC 2 cut(s) 277, 451
Hpy188I TCNGA 4 cut(s) 7, 163, 238, 297
Hpy188III TCNNGA 3 cut(s) 175, 329, 361
Hpy8I GTNNAC 2 cut(s) 277, 451
Hpy99I CGWCG 5 cut(s) 27, 85, 281, 292, 301
HpyAV CCTTC 4 cut(s) 322, 371, 461, 545
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 3 cut(s) 124, 279, 299
HpyCH4V TGCA 1 cut(s) 350
HpyF3I CTNAG 2 cut(s) 30, 230
HpySE526I ACGT 3 cut(s) 124, 279, 299
Hsp92I GRCGYC 1 cut(s) 308
Hsp92II CATG 4 cut(s) 218, 332, 406, 570
Kpn2I TCCGGA 1 cut(s) 174
Kzo9I GATC 3 cut(s) 26, 110, 416
LmnI GCTCC 5 cut(s) 161, 177, 177, 226, 238
LweI GCATC 3 cut(s) 143, 337, 509
MaeII ACGT 3 cut(s) 124, 279, 299
MaeIII GTNAC 2 cut(s) 34, 513
MalI GATC 3 cut(s) 28, 112, 418
MboI GATC 3 cut(s) 26, 110, 416
MboII GAAGA 3 cut(s) 103, 247, 440
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 2 cut(s) 168, 182
MluCI AATT 1 cut(s) 618
MmeI TCCRAC 2 cut(s) 216, 320
MroI TCCGGA 1 cut(s) 174
MseI TTAA 2 cut(s) 576, 621
MspI CCGG 8 cut(s) 19, 49, 58, 88, 175, 242, 259, 392
MspR9I CCNGG 2 cut(s) 58, 387
MvaI CCWGG 1 cut(s) 387
NciI CCSGG 1 cut(s) 58
NcoI CCATGG 1 cut(s) 402
NdeII GATC 3 cut(s) 26, 110, 416
NlaIII CATG 4 cut(s) 218, 332, 406, 570
NlaIV GGNNCC 4 cut(s) 56, 179, 210, 240
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 218
NspV TTCGAA 1 cut(s) 65
PagI TCATGA 1 cut(s) 328
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PfeI GAWTC 1 cut(s) 142
PfoI TCCNGGA 1 cut(s) 385
PinAI ACCGGT 1 cut(s) 391
PkrI GCNGC 1 cut(s) 213
Ple19I CGATCG 1 cut(s) 113
PpuMI RGGWCCY 1 cut(s) 54
PshBI ATTAAT 1 cut(s) 621
Psp1406I AACGTT 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 4 cut(s) 56, 179, 210, 240
PspPI GGNCC 3 cut(s) 54, 389, 399
PspPPI RGGWCCY 1 cut(s) 54
PsuI RGATCY 1 cut(s) 416
PvuI CGATCG 1 cut(s) 113
RgaI GCGATCGC 1 cut(s) 113
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 2 cut(s) 576, 621
SatI GCNGC 1 cut(s) 212
Sau3AI GATC 3 cut(s) 26, 110, 416
Sau96I GGNCC 3 cut(s) 54, 389, 399
ScrFI CCNGG 2 cut(s) 58, 387
SduI GDGCHC 2 cut(s) 168, 182
SfaAI GCGATCGC 1 cut(s) 113
SfaNI GCATC 3 cut(s) 143, 337, 509
SfcI CTRYAG 1 cut(s) 420
SfuI TTCGAA 1 cut(s) 65
SgfI GCGATCGC 1 cut(s) 113
SinI GGWCC 2 cut(s) 54, 389
SmlI CTYRAG 2 cut(s) 146, 488
SmoI CTYRAG 2 cut(s) 146, 488
Sse9I AATT 1 cut(s) 618
SsiI CCGC 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 56, 385
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 3 cut(s) 127, 282, 302
TaqI TCGA 2 cut(s) 65, 276
TasI AATT 1 cut(s) 618
TauI GCSGC 1 cut(s) 214
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 2 cut(s) 576, 621
Tru9I TTAA 2 cut(s) 576, 621
TseFI GTSAC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 555
VpaK11BI GGWCC 2 cut(s) 54, 389
VspI ATTAAT 1 cut(s) 621
XceI RCATGY 1 cut(s) 218
XcmI CCANNNNNNNNNTGG 1 cut(s) 409
XmiI GTMKAC 2 cut(s) 276, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.