MD04G1078000.v1.1

At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011189
Physical Location & Seq
Reverse (-)
10686063 .. 10688700
2638 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1078000.v1.1.491

Sequence Viewer

Length: 828 bp
ATGTCAGAAAGGGGTCTCCGGCGACGATCTCAAGTGAGCGGAGGCCAGAAAACCAGACCGCCGAACCTATCTCCGTCGACGCAACGGAGGACCCATCAATCTCGGCGATCGCCCAAGCAGCACCACCCGCACCACCAGCACCACCAGCACCATTCCAAGCCTATTGAGATTCTCAAGCGATGCTCTTCCGTTCCCGTATTGGTGGGGTCAATTGGCAGCGATTTCTACGAGGAGGAGGAGAAGGAGGGTCTTCGTGGTCCGGCCATGCTGTCGGAGCCGGAGGGTGTTTTGTTCAGGCCTCACACTTGTACGGACATCTTTGCTTCCTCTCCTTCTCTGATGATGGTGCAGCCCCTGCCCTTCTCTCCCCATAAGAACGAGGGATACAAGAAAGATGCAAAGGTGGTGTTGAATGTGTCAGTTGAAGGAAGCCCCGGACCGGTGCGCGCCATGGTTAAATTAGGATCTACAGTGGAAGAAACAATTAAGCTTGTTGTAGACAAATATAGCAAAGAAGGTCGAACTCCGAAGCTTGATCACGAGGCATTGTCCTTTGAGTTGCATCAGTCTTACTTCAGCCTTCAAAGTTTGGATAAATCTGAAGTAATTGGAGATGTCGGTAGCCGAAGCTTCTATCTTAGAAAAAGCAGCAGCAGCAGCAATGGAGTATCAACTTCTTTAATCACTGAAGTCGTTCCTGTGAGAGAAAACACTCCTCCAGCTGCTCCGCGCCCTGTATTTTTACTACCGAATTTCATCGCCCGTAAACTTGGCAAAATCGCCAGGAGGACGCATAAGCTTTGGAGAGTCATAATCTGCATCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

276

Amino Acids

30.86

Weight (kDa)

9.94

Isoelectric Point (pI)

74.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 131 - 214 1.7e-33 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 39
AccI GTMKAC 2 cut(s) 77, 498
AccII CGCG 2 cut(s) 447, 730
AciI CCGC 4 cut(s) 39, 59, 128, 728
AclWI GGATC 1 cut(s) 472
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 1 cut(s) 751
AcuI CTGAAG 3 cut(s) 559, 621, 708
AfaI GTAC 1 cut(s) 310
AfiI CCNNNNNNNGG 1 cut(s) 439
AgeI ACCGGT 1 cut(s) 439
AgsI TTSAA 3 cut(s) 412, 425, 584
AjnI CCWGG 1 cut(s) 782
AluBI AGCT 5 cut(s) 490, 532, 630, 722, 799
AluI AGCT 5 cut(s) 490, 532, 630, 722, 799
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 1 cut(s) 472
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 3 cut(s) 43, 261, 296
ApeKI GCWGC 8 cut(s) 118, 216, 349, 648, 651, 654, 657, 722
ApoI RAATTY 1 cut(s) 751
AsiGI ACCGGT 1 cut(s) 439
AsiSI GCGATCGC 1 cut(s) 110
Asp700I GAANNNNTTC 1 cut(s) 693
AspLEI GCGC 3 cut(s) 447, 449, 732
AspS9I GGNCC 3 cut(s) 90, 257, 437
AsuC2I CCSGG 1 cut(s) 435
AvaII GGWCC 3 cut(s) 90, 257, 437
BauI CACGAG 1 cut(s) 539
BbsI GAAGAC 1 cut(s) 242
BbvI GCAGC 8 cut(s) 130, 228, 361, 660, 663, 666, 669, 709
BccI CCATC 2 cut(s) 102, 337
BciT130I CCWGG 1 cut(s) 784
BciVI GTATCC 1 cut(s) 377
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 1 cut(s) 435
BcoDI GTCTC 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 468
BfuI GTATCC 1 cut(s) 377
BisI GCNGC 8 cut(s) 119, 217, 350, 649, 652, 655, 658, 723
BlsI GCNGC 8 cut(s) 120, 218, 351, 650, 653, 656, 659, 724
Bme1390I CCNGG 2 cut(s) 435, 784
Bme18I GGWCC 3 cut(s) 90, 257, 437
BmgT120I GGNCC 3 cut(s) 90, 257, 437
BmiI GGNNCC 2 cut(s) 92, 276
BmrFI CCNGG 2 cut(s) 435, 784
BmsI GCATC 3 cut(s) 170, 385, 571
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 702
BpuEI CTTGAG 2 cut(s) 15, 158
BpuMI CCSGG 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 433, 450
BsaWI WCCGGW 1 cut(s) 439
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse118I RCCGGY 1 cut(s) 439
Bse3DI GCAATG 1 cut(s) 667
BseBI CCWGG 1 cut(s) 784
BseDI CCNNGG 2 cut(s) 433, 450
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMI GCAATG 1 cut(s) 667
BsePI GCGCGC 1 cut(s) 445
BseRI GAGGAG 4 cut(s) 245, 248, 251, 705
BseXI GCAGC 8 cut(s) 130, 228, 361, 660, 663, 666, 669, 709
BsgI GTGCAG 1 cut(s) 368
Bsh1236I CGCG 2 cut(s) 447, 730
Bsh1285I CGRYCG 1 cut(s) 110
BshFI GGCC 3 cut(s) 45, 263, 298
BshTI ACCGGT 1 cut(s) 439
BsiEI CGRYCG 1 cut(s) 110
BsiSI CCGG 5 cut(s) 19, 260, 278, 435, 440
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 1 cut(s) 20
BsnI GGCC 3 cut(s) 45, 263, 298
Bso31I GGTCTC 1 cut(s) 20
Bsp143I GATC 4 cut(s) 26, 107, 464, 535
Bsp19I CCATGG 1 cut(s) 450
BspACI CCGC 4 cut(s) 39, 59, 128, 728
BspANI GGCC 3 cut(s) 45, 263, 298
BspFNI CGCG 2 cut(s) 447, 730
BspLI GGNNCC 2 cut(s) 92, 276
BspPI GGATC 1 cut(s) 472
BspQI GCTCTTC 1 cut(s) 190
BspTNI GGTCTC 1 cut(s) 20
BsrBI CCGCTC 1 cut(s) 39
BsrDI GCAATG 1 cut(s) 667
BsrFI RCCGGY 1 cut(s) 439
BssAI RCCGGY 1 cut(s) 439
BssECI CCNNGG 2 cut(s) 433, 450
BssHII GCGCGC 1 cut(s) 445
BssMI GATC 4 cut(s) 26, 107, 464, 535
BssSI CACGAG 1 cut(s) 539
BssT1I CCWWGG 1 cut(s) 450
Bst2BI CACGAG 1 cut(s) 539
Bst2UI CCWGG 1 cut(s) 784
Bst4CI ACNGT 1 cut(s) 472
Bst6I CTCTTC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 355
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 1 cut(s) 638
BstDSI CCRYGG 1 cut(s) 450
BstFNI CGCG 2 cut(s) 447, 730
BstHHI GCGC 3 cut(s) 447, 449, 732
BstKTI GATC 4 cut(s) 29, 110, 467, 538
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 4 cut(s) 26, 107, 464, 535
BstMCI CGRYCG 1 cut(s) 110
BstMWI GCNNNNNNNGC 8 cut(s) 118, 127, 136, 145, 274, 355, 654, 657
BstNI CCWGG 1 cut(s) 784
BstSCI CCNGG 2 cut(s) 433, 782
BstSFI CTRYAG 1 cut(s) 468
BstUI CGCG 2 cut(s) 447, 730
BstV1I GCAGC 8 cut(s) 130, 228, 361, 660, 663, 666, 669, 709
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 464
BstYI RGATCY 1 cut(s) 464
BsuI GTATCC 1 cut(s) 377
BsuRI GGCC 3 cut(s) 45, 263, 298
BtgI CCRYGG 1 cut(s) 450
BtgZI GCGATG 2 cut(s) 193, 742
BtsIMutI CAGTG 2 cut(s) 477, 684
Cac8I GCNNGC 1 cut(s) 447
CaiI CAGNNNCTG 1 cut(s) 355
CfoI GCGC 3 cut(s) 447, 449, 732
Cfr10I RCCGGY 1 cut(s) 439
Cfr13I GGNCC 3 cut(s) 90, 257, 437
CpoI CGGWCCG 1 cut(s) 437
CseI GACGC 2 cut(s) 88, 799
Csp6I GTAC 1 cut(s) 309
CspAI ACCGGT 1 cut(s) 439
CspI CGGWCCG 1 cut(s) 437
CviAII CATG 2 cut(s) 265, 451
CviQI GTAC 1 cut(s) 309
DdeI CTNAG 1 cut(s) 638
DpnI GATC 4 cut(s) 28, 109, 466, 537
DpnII GATC 4 cut(s) 26, 107, 464, 535
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 450
Eco147I AGGCCT 1 cut(s) 298
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 3 cut(s) 90, 257, 437
Eco57I CTGAAG 3 cut(s) 559, 621, 708
EcoO109I RGGNCCY 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 782
EcoT14I CCWWGG 1 cut(s) 450
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 2 cut(s) 268, 454
FaiI YATR 6 cut(s) 266, 372, 452, 507, 795, 812
FatI CATG 2 cut(s) 264, 450
FauI CCCGC 1 cut(s) 135
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 2 cut(s) 77, 498
Fnu4HI GCNGC 8 cut(s) 119, 217, 350, 649, 652, 655, 658, 723
Fsp4HI GCNGC 8 cut(s) 119, 217, 350, 649, 652, 655, 658, 723
GlaI GCGC 3 cut(s) 446, 448, 731
GluI GCNGC 8 cut(s) 119, 217, 350, 649, 652, 655, 658, 723
GsuI CTGGAG 1 cut(s) 702
HaeIII GGCC 3 cut(s) 45, 263, 298
HapII CCGG 5 cut(s) 19, 260, 278, 435, 440
HgaI GACGC 2 cut(s) 88, 799
HhaI GCGC 3 cut(s) 447, 449, 732
Hin1II CATG 2 cut(s) 268, 454
Hin6I GCGC 3 cut(s) 445, 447, 730
HinP1I GCGC 3 cut(s) 445, 447, 730
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HindIII AAGCTT 4 cut(s) 488, 530, 628, 797
HinfI GANTC 2 cut(s) 169, 807
HpaII CCGG 5 cut(s) 19, 260, 278, 435, 440
Hpy166II GTNNAC 3 cut(s) 78, 499, 767
Hpy188I TCNGA 5 cut(s) 7, 274, 339, 528, 601
Hpy188III TCNNGA 1 cut(s) 539
Hpy8I GTNNAC 3 cut(s) 78, 499, 767
Hpy99I CGWCG 3 cut(s) 27, 79, 82
HpyAV CCTTC 6 cut(s) 235, 342, 370, 419, 509, 590
HpyCH4III ACNGT 1 cut(s) 472
HpyCH4V TGCA 4 cut(s) 349, 398, 562, 819
HpyF10VI GCNNNNNNNGC 8 cut(s) 118, 127, 136, 145, 274, 355, 654, 657
HpyF3I CTNAG 1 cut(s) 638
Hsp92II CATG 2 cut(s) 268, 454
HspAI GCGC 3 cut(s) 445, 447, 730
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 4 cut(s) 26, 107, 464, 535
LguI GCTCTTC 1 cut(s) 190
LmnI GCTCC 2 cut(s) 274, 730
Lsp1109I GCAGC 8 cut(s) 130, 228, 361, 660, 663, 666, 669, 709
LweI GCATC 3 cut(s) 170, 385, 571
MalI GATC 4 cut(s) 28, 109, 466, 537
MbiI CCGCTC 1 cut(s) 39
MboI GATC 4 cut(s) 26, 107, 464, 535
MboII GAAGA 3 cut(s) 177, 242, 488
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 464
MluCI AATT 5 cut(s) 210, 458, 483, 606, 751
MlyI GAGTC 1 cut(s) 816
MmeI TCCRAC 1 cut(s) 252
MroXI GAANNNNTTC 1 cut(s) 693
MseI TTAA 3 cut(s) 456, 486, 680
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 5 cut(s) 19, 260, 278, 435, 440
MspR9I CCNGG 2 cut(s) 435, 784
MunI CAATTG 1 cut(s) 210
MvaI CCWGG 1 cut(s) 784
MvnI CGCG 2 cut(s) 447, 730
MwoI GCNNNNNNNGC 8 cut(s) 118, 127, 136, 145, 274, 355, 654, 657
NciI CCSGG 1 cut(s) 435
NcoI CCATGG 1 cut(s) 450
NdeII GATC 4 cut(s) 26, 107, 464, 535
NlaIII CATG 2 cut(s) 268, 454
NlaIV GGNNCC 2 cut(s) 92, 276
NmeAIII GCCGAG 1 cut(s) 82
PauI GCGCGC 1 cut(s) 445
PceI AGGCCT 1 cut(s) 298
PciSI GCTCTTC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 1 cut(s) 169
PinAI ACCGGT 1 cut(s) 439
PkrI GCNGC 8 cut(s) 120, 218, 351, 650, 653, 656, 659, 724
Ple19I CGATCG 1 cut(s) 110
PleI GAGTC 1 cut(s) 815
PpsI GAGTC 1 cut(s) 815
PpuMI RGGWCCY 1 cut(s) 90
Psp5II RGGWCCY 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 782
PspGI CCWGG 1 cut(s) 782
PspN4I GGNNCC 2 cut(s) 92, 276
PspPI GGNCC 3 cut(s) 90, 257, 437
PspPPI RGGWCCY 1 cut(s) 90
PsrI GAACNNNNNNTAC 2 cut(s) 368, 400
PstNI CAGNNNCTG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 464
PteI GCGCGC 1 cut(s) 445
PvuI CGATCG 1 cut(s) 110
PvuII CAGCTG 1 cut(s) 722
RgaI GCGATCGC 1 cut(s) 110
RsaI GTAC 1 cut(s) 310
RsaNI GTAC 1 cut(s) 309
Rsr2I CGGWCCG 1 cut(s) 437
RsrII CGGWCCG 1 cut(s) 437
SalI GTCGAC 1 cut(s) 76
SapI GCTCTTC 1 cut(s) 190
SaqAI TTAA 3 cut(s) 456, 486, 680
SatI GCNGC 8 cut(s) 119, 217, 350, 649, 652, 655, 658, 723
Sau3AI GATC 4 cut(s) 26, 107, 464, 535
Sau96I GGNCC 3 cut(s) 90, 257, 437
SchI GAGTC 1 cut(s) 816
ScrFI CCNGG 2 cut(s) 435, 784
SetI ASST 8 cut(s) 69, 405, 492, 520, 534, 632, 724, 801
SfaAI GCGATCGC 1 cut(s) 110
SfaNI GCATC 3 cut(s) 170, 385, 571
SfcI CTRYAG 1 cut(s) 468
SgfI GCGATCGC 1 cut(s) 110
SgrDI CGTCGACG 1 cut(s) 76
SinI GGWCC 3 cut(s) 90, 257, 437
SmlI CTYRAG 2 cut(s) 30, 173
SmoI CTYRAG 2 cut(s) 30, 173
Sse9I AATT 5 cut(s) 210, 458, 483, 606, 751
SseBI AGGCCT 1 cut(s) 298
SsiI CCGC 4 cut(s) 39, 59, 128, 728
StuI AGGCCT 1 cut(s) 298
StyD4I CCNGG 2 cut(s) 433, 782
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 1 cut(s) 472
TaqI TCGA 2 cut(s) 77, 520
TasI AATT 5 cut(s) 210, 458, 483, 606, 751
TfiI GAWTC 1 cut(s) 169
Tru1I TTAA 3 cut(s) 456, 486, 680
Tru9I TTAA 3 cut(s) 456, 486, 680
TscAI CASTG 2 cut(s) 477, 691
TseI GCWGC 8 cut(s) 118, 216, 349, 648, 651, 654, 657, 722
TspDTI ATGAA 1 cut(s) 745
TspGWI ACGGA 4 cut(s) 63, 100, 178, 326
TspRI CASTG 2 cut(s) 477, 691
VpaK11BI GGWCC 3 cut(s) 90, 257, 437
XapI RAATTY 1 cut(s) 751
XmiI GTMKAC 2 cut(s) 77, 498
XmnI GAANNNNTTC 1 cut(s) 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.