FvH4_5g00820

At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011189
Physical Location & Seq
Forward (+)
468356 .. 470695
2340 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00820.t1

Sequence Viewer

Length: 783 bp
ATGTCAGAGAGGGTTCTCCGGCGACGATCTCAGGCGACGGGCGGTAGTGGCAGCCGGAGGACCCGCCTCTCGAACCCTTCACCATCGCCGCACCGGAAGACCCCACAAACTAAGCGATCGCCCAAGCCACGTTCCAAGCCTGTAAAGATTCTTAAGCGATGCTCGTCCGAGCCTCTGCTCCGGAGTCCCGTCAGCGATTTCGGCGACGAGGTGGTGCCGCATGTTGGCCGGGAGTTGAGGTCGGAGCCGGATGGAGTTCTTTTCCGGCCACAAACTTGTGTTGACGTGTTTGCTTCGTCTCCGACGTTGATGATACCTTTCTCTCCCAATAATCATGAGGCATACAAGAAAGATGCAAAGGTGGTATTGAATGTTTCCGTTGAAGGAAGTCCAGGACCGGTGAGGGCCATGGTGAAGTTGGGATCTACGGTGGAAGAGACGATCAAGCTTGTTGTAGACAAATATAGCAAAGAAGGGAGGACTCCCAAGCTTCTTAAGGATGAGGCATCGTCCTTTGAGTTACACCACTCCTACTTCAGCCTTCAAAGTTTGGATAAATCAGACTTGATTGGGGATGTTGGTAGCAGAAGCTTCTATCTTCGAAAGAGCAGCAGCAAGAATGGAGGATCTACTGCTTCTTTGGAAGTCATTCCCGTGAGAGCAAACTCTCCTCCACCTGCTCCGCGTCCAGGATTGTTACTTCCAAATTTTATTGCTCGTAAACTCGCTAAACTTGTTAGAAGAACTCATAGACTTTGGAAGGTCATAGTCTGTATGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

261

Amino Acids

28.81

Weight (kDa)

10.52

Isoelectric Point (pI)

70.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 117 - 201 2.1e-34 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 685
Acc36I ACCTGC 1 cut(s) 685
AccB1I GGYRCC 1 cut(s) 214
AccI GTMKAC 1 cut(s) 456
AccII CGCG 1 cut(s) 685
AccIII TCCGGA 1 cut(s) 180
AciI CCGC 5 cut(s) 42, 64, 89, 218, 683
AclWI GGATC 2 cut(s) 430, 634
AcoI YGGCCR 2 cut(s) 226, 266
AcsI RAATTY 1 cut(s) 706
AcuI CTGAAG 1 cut(s) 520
AfiI CCNNNNNNNGG 2 cut(s) 224, 689
AflII CTTAAG 2 cut(s) 152, 494
AflIII ACRYGT 1 cut(s) 285
AgeI ACCGGT 1 cut(s) 397
AgsI TTSAA 3 cut(s) 370, 383, 545
AjiI CACGTC 1 cut(s) 286
AjnI CCWGG 2 cut(s) 391, 688
AluBI AGCT 3 cut(s) 448, 490, 591
AluI AGCT 3 cut(s) 448, 490, 591
Alw26I GTCTC 2 cut(s) 303, 431
AlwI GGATC 2 cut(s) 430, 634
Aor13HI TCCGGA 1 cut(s) 180
AoxI GGCC 3 cut(s) 226, 266, 405
ApeKI GCWGC 3 cut(s) 51, 609, 612
ApoI RAATTY 1 cut(s) 706
AsiGI ACCGGT 1 cut(s) 397
AsiSI GCGATCGC 1 cut(s) 119
Asp700I GAANNNNTTC 1 cut(s) 648
AspS9I GGNCC 3 cut(s) 60, 395, 405
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 3 cut(s) 72, 412, 424
AsuII TTCGAA 1 cut(s) 601
AvaII GGWCC 2 cut(s) 60, 395
BanI GGYRCC 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 104
BbvI GCAGC 3 cut(s) 63, 621, 624
BccI CCATC 2 cut(s) 91, 245
BciT130I CCWGG 2 cut(s) 393, 690
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 2 cut(s) 303, 431
BfrI CTTAAG 2 cut(s) 152, 494
BfuAI ACCTGC 1 cut(s) 685
BisI GCNGC 5 cut(s) 52, 89, 218, 610, 613
BlsI GCNGC 5 cut(s) 53, 90, 219, 611, 614
Bme1390I CCNGG 3 cut(s) 230, 393, 690
Bme18I GGWCC 2 cut(s) 60, 395
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 3 cut(s) 60, 395, 405
BmiI GGNNCC 3 cut(s) 62, 216, 246
BmrFI CCNGG 3 cut(s) 230, 393, 690
BmsI GCATC 3 cut(s) 149, 343, 515
BpiI GAAGAC 1 cut(s) 104
Bpu14I TTCGAA 1 cut(s) 601
BpuMI CCSGG 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 408
BsaWI WCCGGW 3 cut(s) 93, 180, 397
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 689
Bse118I RCCGGY 1 cut(s) 397
BseAI TCCGGA 1 cut(s) 180
BseBI CCWGG 2 cut(s) 393, 690
BseDI CCNNGG 1 cut(s) 408
BseGI GGATG 3 cut(s) 256, 505, 580
BseLI CCNNNNNNNGG 2 cut(s) 224, 689
BseMII CTCAG 1 cut(s) 44
BseRI GAGGAG 1 cut(s) 660
BseXI GCAGC 3 cut(s) 63, 621, 624
Bsh1236I CGCG 1 cut(s) 685
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 3 cut(s) 228, 268, 407
BshNI GGYRCC 1 cut(s) 214
BshTI ACCGGT 1 cut(s) 397
BsiEI CGRYCG 1 cut(s) 119
BsiSI CCGG 8 cut(s) 19, 55, 94, 181, 229, 248, 265, 398
BslFI GGGAC 1 cut(s) 171
BslI CCNNNNNNNGG 2 cut(s) 224, 689
BsmAI GTCTC 2 cut(s) 303, 431
BsmBI CGTCTC 2 cut(s) 303, 431
BsmFI GGGAC 1 cut(s) 171
BsnI GGCC 3 cut(s) 228, 268, 407
Bsp119I TTCGAA 1 cut(s) 601
Bsp13I TCCGGA 1 cut(s) 180
Bsp143I GATC 5 cut(s) 26, 116, 422, 441, 626
Bsp19I CCATGG 1 cut(s) 408
BspACI CCGC 5 cut(s) 42, 64, 89, 218, 683
BspANI GGCC 3 cut(s) 228, 268, 407
BspCNI CTCAG 1 cut(s) 43
BspEI TCCGGA 1 cut(s) 180
BspFNI CGCG 1 cut(s) 685
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 3 cut(s) 62, 216, 246
BspMI ACCTGC 1 cut(s) 685
BspPI GGATC 2 cut(s) 430, 634
BspT104I TTCGAA 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 214
BspTI CTTAAG 2 cut(s) 152, 494
BsrFI RCCGGY 1 cut(s) 397
BssAI RCCGGY 1 cut(s) 397
BssECI CCNNGG 1 cut(s) 408
BssMI GATC 5 cut(s) 26, 116, 422, 441, 626
BssT1I CCWWGG 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 393, 690
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 429
BstAFI CTTAAG 2 cut(s) 152, 494
BstBI TTCGAA 1 cut(s) 601
BstDEI CTNAG 2 cut(s) 30, 111
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 3 cut(s) 256, 505, 580
BstFNI CGCG 1 cut(s) 685
BstKTI GATC 5 cut(s) 29, 119, 425, 444, 629
BstMAI GTCTC 2 cut(s) 303, 431
BstMBI GATC 5 cut(s) 26, 116, 422, 441, 626
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 2 cut(s) 48, 201
BstNI CCWGG 2 cut(s) 393, 690
BstNSI RCATGY 1 cut(s) 224
BstSCI CCNGG 3 cut(s) 228, 391, 688
BstUI CGCG 1 cut(s) 685
BstV1I GCAGC 3 cut(s) 63, 621, 624
BstV2I GAAGAC 1 cut(s) 104
BstX2I RGATCY 2 cut(s) 422, 626
BstYI RGATCY 2 cut(s) 422, 626
BsuRI GGCC 3 cut(s) 228, 268, 407
BtgI CCRYGG 1 cut(s) 408
BtgZI GCGATG 2 cut(s) 69, 172
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 3 cut(s) 256, 505, 580
BveI ACCTGC 1 cut(s) 685
CciI TCATGA 1 cut(s) 334
Cfr10I RCCGGY 1 cut(s) 397
Cfr13I GGNCC 3 cut(s) 60, 395, 405
CseI GACGC 1 cut(s) 674
CspAI ACCGGT 1 cut(s) 397
CviAII CATG 3 cut(s) 221, 335, 409
DdeI CTNAG 2 cut(s) 30, 111
DpnI GATC 5 cut(s) 28, 118, 424, 443, 628
DpnII GATC 5 cut(s) 26, 116, 422, 441, 626
EaeI YGGCCR 2 cut(s) 226, 266
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 408
Eco47I GGWCC 2 cut(s) 60, 395
Eco57I CTGAAG 1 cut(s) 520
EcoO109I RGGNCCY 1 cut(s) 60
EcoRII CCWGG 2 cut(s) 391, 688
EcoT14I CCWWGG 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 408
Esp3I CGTCTC 2 cut(s) 303, 431
FaeI CATG 3 cut(s) 224, 338, 412
FaiI YATR 8 cut(s) 222, 336, 343, 410, 465, 750, 767, 776
FaqI GGGAC 1 cut(s) 171
FatI CATG 3 cut(s) 220, 334, 408
FauI CCCGC 1 cut(s) 71
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 5 cut(s) 52, 89, 218, 610, 613
FokI GGATG 3 cut(s) 263, 512, 587
Fsp4HI GCNGC 5 cut(s) 52, 89, 218, 610, 613
GluI GCNGC 5 cut(s) 52, 89, 218, 610, 613
HaeIII GGCC 3 cut(s) 228, 268, 407
HapII CCGG 8 cut(s) 19, 55, 94, 181, 229, 248, 265, 398
HgaI GACGC 1 cut(s) 674
Hin1II CATG 3 cut(s) 224, 338, 412
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 3 cut(s) 446, 488, 589
HinfI GANTC 3 cut(s) 148, 184, 481
HpaII CCGG 8 cut(s) 19, 55, 94, 181, 229, 248, 265, 398
HphI GGTGA 3 cut(s) 72, 412, 424
Hpy166II GTNNAC 3 cut(s) 283, 457, 722
Hpy188I TCNGA 5 cut(s) 7, 169, 244, 303, 562
Hpy188III TCNNGA 3 cut(s) 70, 181, 335
Hpy8I GTNNAC 3 cut(s) 283, 457, 722
Hpy99I CGWCG 4 cut(s) 27, 40, 209, 307
HpyAV CCTTC 5 cut(s) 87, 377, 467, 551, 754
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 3 cut(s) 130, 285, 305
HpyCH4V TGCA 1 cut(s) 356
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 201
HpyF3I CTNAG 2 cut(s) 30, 111
HpySE526I ACGT 3 cut(s) 130, 285, 305
Hsp92II CATG 3 cut(s) 224, 338, 412
Kpn2I TCCGGA 1 cut(s) 180
Kzo9I GATC 5 cut(s) 26, 116, 422, 441, 626
LmnI GCTCC 3 cut(s) 183, 244, 685
Lsp1109I GCAGC 3 cut(s) 63, 621, 624
LweI GCATC 3 cut(s) 149, 343, 515
MaeII ACGT 3 cut(s) 130, 285, 305
MaeIII GTNAC 2 cut(s) 519, 696
MalI GATC 5 cut(s) 28, 118, 424, 443, 628
MboI GATC 5 cut(s) 26, 116, 422, 441, 626
MboII GAAGA 4 cut(s) 109, 446, 590, 753
MflI RGATCY 2 cut(s) 422, 626
MluCI AATT 1 cut(s) 706
MlyI GAGTC 2 cut(s) 193, 475
MmeI TCCRAC 2 cut(s) 222, 326
MroI TCCGGA 1 cut(s) 180
MroXI GAANNNNTTC 1 cut(s) 648
MseI TTAA 2 cut(s) 153, 495
MslI CAYNNNNRTG 1 cut(s) 653
MspCI CTTAAG 2 cut(s) 152, 494
MspI CCGG 8 cut(s) 19, 55, 94, 181, 229, 248, 265, 398
MspR9I CCNGG 3 cut(s) 230, 393, 690
MvaI CCWGG 2 cut(s) 393, 690
MvnI CGCG 1 cut(s) 685
MwoI GCNNNNNNNGC 2 cut(s) 48, 201
NciI CCSGG 1 cut(s) 230
NcoI CCATGG 1 cut(s) 408
NdeII GATC 5 cut(s) 26, 116, 422, 441, 626
NlaIII CATG 3 cut(s) 224, 338, 412
NlaIV GGNNCC 3 cut(s) 62, 216, 246
NspI RCATGY 1 cut(s) 224
NspV TTCGAA 1 cut(s) 601
PagI TCATGA 1 cut(s) 334
PaqCI CACCTGC 1 cut(s) 685
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 1 cut(s) 148
PfoI TCCNGGA 2 cut(s) 391, 688
PinAI ACCGGT 1 cut(s) 397
PkrI GCNGC 5 cut(s) 53, 90, 219, 611, 614
Ple19I CGATCG 1 cut(s) 119
PleI GAGTC 2 cut(s) 192, 475
PpsI GAGTC 2 cut(s) 192, 475
PpuMI RGGWCCY 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 391, 688
PspGI CCWGG 2 cut(s) 391, 688
PspN4I GGNNCC 3 cut(s) 62, 216, 246
PspPI GGNCC 3 cut(s) 60, 395, 405
PspPPI RGGWCCY 1 cut(s) 60
PsuI RGATCY 2 cut(s) 422, 626
PvuI CGATCG 1 cut(s) 119
RgaI GCGATCGC 1 cut(s) 119
RseI CAYNNNNRTG 1 cut(s) 653
SaqAI TTAA 2 cut(s) 153, 495
SatI GCNGC 5 cut(s) 52, 89, 218, 610, 613
Sau3AI GATC 5 cut(s) 26, 116, 422, 441, 626
Sau96I GGNCC 3 cut(s) 60, 395, 405
SchI GAGTC 2 cut(s) 193, 475
ScrFI CCNGG 3 cut(s) 230, 393, 690
SfaAI GCGATCGC 1 cut(s) 119
SfaNI GCATC 3 cut(s) 149, 343, 515
SfuI TTCGAA 1 cut(s) 601
SgfI GCGATCGC 1 cut(s) 119
SinI GGWCC 2 cut(s) 60, 395
SmiMI CAYNNNNRTG 1 cut(s) 653
SmlI CTYRAG 2 cut(s) 152, 494
SmoI CTYRAG 2 cut(s) 152, 494
Sse9I AATT 1 cut(s) 706
SsiI CCGC 5 cut(s) 42, 64, 89, 218, 683
StyD4I CCNGG 3 cut(s) 228, 391, 688
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 3 cut(s) 133, 288, 308
TaqI TCGA 2 cut(s) 71, 601
TasI AATT 1 cut(s) 706
TauI GCSGC 2 cut(s) 91, 220
TfiI GAWTC 1 cut(s) 148
Tru1I TTAA 2 cut(s) 153, 495
Tru9I TTAA 2 cut(s) 153, 495
TseI GCWGC 3 cut(s) 51, 609, 612
TspGWI ACGGA 1 cut(s) 367
Vha464I CTTAAG 2 cut(s) 152, 494
VpaK11BI GGWCC 2 cut(s) 60, 395
XapI RAATTY 1 cut(s) 706
XceI RCATGY 1 cut(s) 224
XcmI CCANNNNNNNNNTGG 1 cut(s) 415
XmiI GTMKAC 1 cut(s) 456
XmnI GAANNNNTTC 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.