AT4G22830

Protein of unknown function (DUF2499)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008707
Physical Location & Seq
Reverse (-)
11990028 .. 11991491
1464 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22830.1

Sequence Viewer

Length: 582 bp
ATGGCGATTGCTTCAACAGCCACCGTTCTTTTCTGCTGCGCCAAACCCAATTTCCGGCGAATTCATTCTGAGAGCCGTCCAGCTTCTTCTCTCACCACCTCTAACGAACCTGTAATTCGGATCCGAAGCCGCAGTTTAATTCATGACCCGGTTGCGCTGTCGGGTCTTATCGGAACCAGTTTGGTTGCGGCTGCTTTCATCACGGCGGGACCAGATGCTACTGCTTTGGCGGCGGTGGTGGATTCTCTTCAGCTAAGCGAACCGTCCAATGCGTTGTCTTTACCCACTTGGGCCGTTCATGTTTCCAGCGTTGTCGAATGGATTACGGCGATGGCATTGGTATGGAAGTATGGAGAAAGAAAGGGTTATGAGTCTTGGAAAGGTCTCTCATGGGGAATGGTACCTTTGCTTGGTGGAGCTCTCTGTGCATGCACATGGCATTTCTTCTACAACGATGAATCTCTTGAGGTGTTGGTCGCTCTTCAGGCAGCACTAACGGTGTTTGGTAATATCACTCTCTGCATTGCTGCCTTTCGGATCAACAAGTTGTCATCCAAAATTGAAGTCTCTGAAAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2499

Protein Analysis

193

Amino Acids

20.74

Weight (kDa)

7.66

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2499 PF10693 91 - 178 4.3e-40 Protein of unknown function (DUF2499)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 400
AccB1I GGYRCC 1 cut(s) 400
AciI CCGC 5 cut(s) 130, 188, 206, 230, 233
AclWI GGATC 3 cut(s) 115, 128, 545
AcsI RAATTY 1 cut(s) 60
AcuI CTGAAG 2 cut(s) 233, 467
AfaI GTAC 1 cut(s) 402
AfiI CCNNNNNNNGG 2 cut(s) 54, 410
AgsI TTSAA 2 cut(s) 15, 563
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
AluBI AGCT 3 cut(s) 83, 253, 419
AluI AGCT 3 cut(s) 83, 253, 419
Alw21I GWGCWC 1 cut(s) 421
Alw26I GTCTC 2 cut(s) 389, 571
AlwI GGATC 3 cut(s) 115, 128, 545
AoxI GGCC 1 cut(s) 291
ApeKI GCWGC 4 cut(s) 36, 191, 488, 527
ApoI RAATTY 1 cut(s) 60
ArsI GACNNNNNNTTYG 2 cut(s) 549, 581
Asp700I GAANNNNTTC 1 cut(s) 64
Asp718I GGTACC 1 cut(s) 400
AspLEI GCGC 2 cut(s) 41, 157
AspS9I GGNCC 2 cut(s) 209, 291
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 85
AvaII GGWCC 1 cut(s) 209
BamHI GGATCC 1 cut(s) 120
BanI GGYRCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 421
Bbv12I GWGCWC 1 cut(s) 421
BbvI GCAGC 4 cut(s) 23, 178, 500, 514
BccI CCATC 1 cut(s) 325
BceAI ACGGC 4 cut(s) 60, 219, 278, 342
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 2 cut(s) 389, 571
BisI GCNGC 7 cut(s) 37, 130, 189, 192, 231, 489, 528
BlpI GCTNAGC 1 cut(s) 254
BlsI GCNGC 7 cut(s) 38, 131, 190, 193, 232, 490, 529
Bme1390I CCNGG 1 cut(s) 149
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 2 cut(s) 209, 291
BmiI GGNNCC 4 cut(s) 122, 175, 210, 402
BmrFI CCNGG 1 cut(s) 149
BmsI GCATC 1 cut(s) 205
Bpu1102I GCTNAGC 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 485
BpuMI CCSGG 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 389
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 410
Bse1I ACTGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 522
BseGI GGATG 1 cut(s) 551
BseLI CCNNNNNNNGG 2 cut(s) 54, 410
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 177
BseXI GCAGC 4 cut(s) 23, 178, 500, 514
BshFI GGCC 1 cut(s) 293
BshNI GGYRCC 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 421
BsiSI CCGG 2 cut(s) 55, 149
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 54, 410
BsmAI GTCTC 2 cut(s) 389, 571
BsmFI GGGAC 1 cut(s) 222
BsnI GGCC 1 cut(s) 293
Bso31I GGTCTC 1 cut(s) 389
Bsp1286I GDGCHC 1 cut(s) 421
Bsp143I GATC 2 cut(s) 120, 537
Bsp1720I GCTNAGC 1 cut(s) 254
BspACI CCGC 5 cut(s) 130, 188, 206, 230, 233
BspANI GGCC 1 cut(s) 293
BspCNI CTCAG 1 cut(s) 61
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 4 cut(s) 122, 175, 210, 402
BspPI GGATC 3 cut(s) 115, 128, 545
BspQI GCTCTTC 1 cut(s) 486
BspT107I GGYRCC 1 cut(s) 400
BspTNI GGTCTC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 522
BsrI ACTGG 1 cut(s) 177
BssMI GATC 2 cut(s) 120, 537
Bst4CI ACNGT 3 cut(s) 25, 264, 499
Bst6I CTCTTC 2 cut(s) 252, 486
BstC8I GCNNGC 1 cut(s) 430
BstDEI CTNAG 2 cut(s) 69, 254
BstF5I GGATG 1 cut(s) 551
BstHHI GCGC 2 cut(s) 41, 157
BstKTI GATC 2 cut(s) 123, 540
BstMAI GTCTC 2 cut(s) 389, 571
BstMBI GATC 2 cut(s) 120, 537
BstMWI GCNNNNNNNGC 4 cut(s) 17, 230, 425, 485
BstNSI RCATGY 1 cut(s) 432
BstSCI CCNGG 1 cut(s) 147
BstV1I GCAGC 4 cut(s) 23, 178, 500, 514
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BsuRI GGCC 1 cut(s) 293
BtgZI GCGATG 1 cut(s) 344
BtsCI GGATG 1 cut(s) 551
Cac8I GCNNGC 1 cut(s) 430
CciI TCATGA 1 cut(s) 142
CfoI GCGC 2 cut(s) 41, 157
Cfr13I GGNCC 2 cut(s) 209, 291
Csp6I GTAC 1 cut(s) 401
CviAII CATG 6 cut(s) 143, 299, 390, 429, 435, 579
CviJI RGCY 9 cut(s) 20, 75, 83, 129, 191, 253, 293, 419, 577
CviKI_1 RGCY 9 cut(s) 20, 75, 83, 129, 191, 253, 293, 419, 577
CviQI GTAC 1 cut(s) 401
DdeI CTNAG 2 cut(s) 69, 254
DpnI GATC 2 cut(s) 122, 539
DpnII GATC 2 cut(s) 120, 537
Eam1104I CTCTTC 2 cut(s) 252, 486
EarI CTCTTC 2 cut(s) 252, 486
Ecl136II GAGCTC 1 cut(s) 419
Eco24I GRGCYC 1 cut(s) 421
Eco31I GGTCTC 1 cut(s) 389
Eco47I GGWCC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 419
Eco57I CTGAAG 2 cut(s) 233, 467
EcoICRI GAGCTC 1 cut(s) 419
EcoRI GAATTC 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 421
FaeI CATG 6 cut(s) 146, 302, 393, 432, 438, 582
FaiI YATR 9 cut(s) 144, 300, 343, 351, 369, 391, 430, 436, 580
FaqI GGGAC 1 cut(s) 222
FatI CATG 6 cut(s) 142, 298, 389, 428, 434, 578
FauI CCCGC 1 cut(s) 199
Fnu4HI GCNGC 7 cut(s) 37, 130, 189, 192, 231, 489, 528
FokI GGATG 1 cut(s) 538
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 7 cut(s) 37, 130, 189, 192, 231, 489, 528
GlaI GCGC 2 cut(s) 40, 156
GluI GCNGC 7 cut(s) 37, 130, 189, 192, 231, 489, 528
HaeIII GGCC 1 cut(s) 293
HapII CCGG 2 cut(s) 55, 149
HhaI GCGC 2 cut(s) 41, 157
Hin1II CATG 6 cut(s) 146, 302, 393, 432, 438, 582
Hin6I GCGC 2 cut(s) 39, 155
HinP1I GCGC 2 cut(s) 39, 155
HinfI GANTC 3 cut(s) 242, 371, 458
HpaII CCGG 2 cut(s) 55, 149
HphI GGTGA 1 cut(s) 85
Hpy188I TCNGA 6 cut(s) 70, 120, 125, 173, 537, 571
Hpy188III TCNNGA 2 cut(s) 143, 464
HpyCH4III ACNGT 3 cut(s) 25, 264, 499
HpyCH4V TGCA 3 cut(s) 428, 432, 522
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 230, 425, 485
HpyF3I CTNAG 2 cut(s) 69, 254
Hsp92II CATG 6 cut(s) 146, 302, 393, 432, 438, 582
HspAI GCGC 2 cut(s) 39, 155
KpnI GGTACC 1 cut(s) 404
Kzo9I GATC 2 cut(s) 120, 537
LguI GCTCTTC 1 cut(s) 486
LmnI GCTCC 1 cut(s) 416
LpnPI CCDG 8 cut(s) 68, 93, 123, 162, 190, 225, 319, 470
Lsp1109I GCAGC 4 cut(s) 23, 178, 500, 514
LweI GCATC 1 cut(s) 205
MalI GATC 2 cut(s) 122, 539
MboI GATC 2 cut(s) 120, 537
MboII GAAGA 4 cut(s) 78, 239, 436, 473
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 421
MluCI AATT 5 cut(s) 49, 60, 114, 138, 558
MlyI GAGTC 1 cut(s) 380
MnlI CCTC 2 cut(s) 109, 460
MroXI GAANNNNTTC 1 cut(s) 64
MseI TTAA 1 cut(s) 137
MslI CAYNNNNRTG 2 cut(s) 340, 433
MspI CCGG 2 cut(s) 55, 149
MspR9I CCNGG 1 cut(s) 149
MwoI GCNNNNNNNGC 4 cut(s) 17, 230, 425, 485
NciI CCSGG 1 cut(s) 149
NdeII GATC 2 cut(s) 120, 537
NlaIII CATG 6 cut(s) 146, 302, 393, 432, 438, 582
NlaIV GGNNCC 4 cut(s) 122, 175, 210, 402
NspI RCATGY 1 cut(s) 432
PaeI GCATGC 1 cut(s) 432
PagI TCATGA 1 cut(s) 142
PciSI GCTCTTC 1 cut(s) 486
PdmI GAANNNNTTC 1 cut(s) 64
PfeI GAWTC 2 cut(s) 242, 458
PkrI GCNGC 7 cut(s) 38, 131, 190, 193, 232, 490, 529
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 421
PspN4I GGNNCC 4 cut(s) 122, 175, 210, 402
PspPI GGNCC 2 cut(s) 209, 291
PsuI RGATCY 1 cut(s) 120
RsaI GTAC 1 cut(s) 402
RsaNI GTAC 1 cut(s) 401
RseI CAYNNNNRTG 2 cut(s) 340, 433
SacI GAGCTC 1 cut(s) 421
SapI GCTCTTC 1 cut(s) 486
SaqAI TTAA 1 cut(s) 137
SatI GCNGC 7 cut(s) 37, 130, 189, 192, 231, 489, 528
Sau3AI GATC 2 cut(s) 120, 537
Sau96I GGNCC 2 cut(s) 209, 291
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 1 cut(s) 149
SduI GDGCHC 1 cut(s) 421
SetI ASST 8 cut(s) 85, 101, 112, 255, 385, 406, 421, 471
SfaNI GCATC 1 cut(s) 205
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 2 cut(s) 340, 433
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
SphI GCATGC 1 cut(s) 432
Sse9I AATT 5 cut(s) 49, 60, 114, 138, 558
SsiI CCGC 5 cut(s) 130, 188, 206, 230, 233
SstI GAGCTC 1 cut(s) 421
StyD4I CCNGG 1 cut(s) 147
TaaI ACNGT 3 cut(s) 25, 264, 499
TaqI TCGA 1 cut(s) 315
TasI AATT 5 cut(s) 49, 60, 114, 138, 558
TauI GCSGC 3 cut(s) 132, 191, 233
TfiI GAWTC 2 cut(s) 242, 458
Tru1I TTAA 1 cut(s) 137
Tru9I TTAA 1 cut(s) 137
TseI GCWGC 4 cut(s) 36, 191, 488, 527
TspDTI ATGAA 5 cut(s) 53, 131, 187, 287, 471
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 1 cut(s) 60
XceI RCATGY 1 cut(s) 432
XmnI GAANNNNTTC 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.