FvH4_5g00940

Ycf49-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008707
Physical Location & Seq
Reverse (-)
550632 .. 552759
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00940.t4

Sequence Viewer

Length: 546 bp
ATGAACTCCTTACTTCACCTCCCTAAACCCCATTTCCCTCCCACCTTCCTTACACACCCAAAACCCCAAACCCAACTCTCAACAAAGTCCTTCACTCAATATGGCACCCTCCTTCATGACCCGAAAACCATGGTGGGTCTACTCGGGGCGGGTCTGACCCTGACTCTGACCGTTATGGCACCCGCCTCAGCCGCTGAGTTACCGTTACTGGATTCTCTGCAGCTCAGTGAACCTGCCAATGCCCTCTCCCTGCCCACCTGGGCCATACACGTGTCAAGTGTCGTTGAGTGGATTACAGCAATGGTGTTGGTGTGGCAGTATGGTGAGAAGTCCGGGTTCGAATCGTGGAAAGGGCTCGCTTGGGGTATGGTTCCTCTTCTTGGTGGAGCATTCTGCGCATGCACTTGGCATTTCTTTTATAACTCGGAGTCTCTTGAGGTATTGGTGGCTCTTCAAGCGGCCCTGACAGTTATGGGCAATGCCACAATGTGTTTTGCTGCATATCGAATTTACAGATCAACTGAAGAACGGTCCAAGAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2499

Protein Analysis

182

Amino Acids

19.88

Weight (kDa)

7.0

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2499 PF10693 81 - 147 3.1e-30 Protein of unknown function (DUF2499)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 420
Acc16I TGCGCA 1 cut(s) 397
Acc36I ACCTGC 1 cut(s) 241
AccB1I GGYRCC 2 cut(s) 104, 178
AccI GTMKAC 1 cut(s) 139
AciI CCGC 4 cut(s) 149, 183, 192, 458
AcsI RAATTY 1 cut(s) 507
AcuI CTGAAG 1 cut(s) 543
AcvI CACGTG 1 cut(s) 271
AdeI CACNNNGTG 1 cut(s) 489
AfiI CCNNNNNNNGG 1 cut(s) 380
AflIII ACRYGT 2 cut(s) 268, 270
AgsI TTSAA 1 cut(s) 455
AjnI CCWGG 1 cut(s) 257
AluBI AGCT 2 cut(s) 223, 541
AluI AGCT 2 cut(s) 223, 541
Alw26I GTCTC 1 cut(s) 435
AlwNI CAGNNNCTG 1 cut(s) 194
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 2 cut(s) 261, 459
ApeKI GCWGC 2 cut(s) 220, 497
ApoI RAATTY 1 cut(s) 507
AspLEI GCGC 1 cut(s) 398
AspS9I GGNCC 3 cut(s) 261, 460, 531
AsuC2I CCSGG 1 cut(s) 334
AsuHPI GGTGA 2 cut(s) 8, 335
AsuII TTCGAA 1 cut(s) 339
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 1 cut(s) 531
BanI GGYRCC 2 cut(s) 104, 178
BanII GRGCYC 1 cut(s) 357
BbrPI CACGTG 1 cut(s) 271
BbvCI CCTCAGC 1 cut(s) 187
BbvI GCAGC 2 cut(s) 232, 484
BciT130I CCWGG 1 cut(s) 259
BcnI CCSGG 1 cut(s) 334
BcoDI GTCTC 1 cut(s) 435
BfmI CTRYAG 1 cut(s) 218
BfuAI ACCTGC 1 cut(s) 241
BisI GCNGC 4 cut(s) 192, 221, 459, 498
BlsI GCNGC 4 cut(s) 193, 222, 460, 499
Bme1390I CCNGG 2 cut(s) 259, 334
Bme18I GGWCC 1 cut(s) 531
BmeT110I CYCGRG 1 cut(s) 143
BmgT120I GGNCC 3 cut(s) 261, 460, 531
BmiI GGNNCC 3 cut(s) 106, 180, 372
BmrFI CCNGG 2 cut(s) 259, 334
Bpu10I CCTNAGC 1 cut(s) 187
Bpu14I TTCGAA 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 334
BsaAI YACGTR 1 cut(s) 271
BsaJI CCNNGG 2 cut(s) 129, 258
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 380
Bse1I ACTGG 1 cut(s) 213
Bse3DI GCAATG 2 cut(s) 306, 484
BseBI CCWGG 1 cut(s) 259
BseDI CCNNGG 2 cut(s) 129, 258
BseLI CCNNNNNNNGG 1 cut(s) 380
BseMI GCAATG 2 cut(s) 306, 484
BseMII CTCAG 3 cut(s) 186, 201, 238
BseNI ACTGG 1 cut(s) 213
BseXI GCAGC 2 cut(s) 232, 484
BshFI GGCC 2 cut(s) 263, 461
BshNI GGYRCC 2 cut(s) 104, 178
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 1 cut(s) 333
BslI CCNNNNNNNGG 1 cut(s) 380
BsmAI GTCTC 1 cut(s) 435
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 2 cut(s) 263, 461
BsoBI CYCGRG 1 cut(s) 143
Bsp119I TTCGAA 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 357
Bsp143I GATC 1 cut(s) 515
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 4 cut(s) 149, 183, 192, 458
BspANI GGCC 2 cut(s) 263, 461
BspCNI CTCAG 3 cut(s) 187, 200, 237
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 3 cut(s) 106, 180, 372
BspMAI CTGCAG 1 cut(s) 222
BspMI ACCTGC 1 cut(s) 241
BspQI GCTCTTC 1 cut(s) 456
BspT104I TTCGAA 1 cut(s) 339
BspT107I GGYRCC 2 cut(s) 104, 178
BsrDI GCAATG 2 cut(s) 306, 484
BsrI ACTGG 1 cut(s) 213
BssECI CCNNGG 2 cut(s) 129, 258
BssMI GATC 1 cut(s) 515
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 259
Bst4CI ACNGT 4 cut(s) 172, 204, 469, 531
Bst6I CTCTTC 2 cut(s) 381, 456
BstBAI YACGTR 1 cut(s) 271
BstBI TTCGAA 1 cut(s) 339
BstC8I GCNNGC 2 cut(s) 357, 400
BstDEI CTNAG 3 cut(s) 187, 195, 224
BstDSI CCRYGG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 1 cut(s) 518
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 1 cut(s) 515
BstMWI GCNNNNNNNGC 3 cut(s) 191, 395, 455
BstNI CCWGG 1 cut(s) 259
BstNSI RCATGY 1 cut(s) 402
BstSCI CCNGG 2 cut(s) 257, 332
BstSFI CTRYAG 1 cut(s) 218
BstV1I GCAGC 2 cut(s) 232, 484
BsuRI GGCC 2 cut(s) 263, 461
BtgI CCRYGG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 232
BveI ACCTGC 1 cut(s) 241
Cac8I GCNNGC 2 cut(s) 357, 400
CaiI CAGNNNCTG 1 cut(s) 194
CciI TCATGA 1 cut(s) 115
CfoI GCGC 1 cut(s) 398
Cfr13I GGNCC 3 cut(s) 261, 460, 531
CviAII CATG 3 cut(s) 116, 130, 399
CviJI RGCY 7 cut(s) 191, 223, 263, 355, 449, 461, 541
CviKI_1 RGCY 7 cut(s) 191, 223, 263, 355, 449, 461, 541
DdeI CTNAG 3 cut(s) 187, 195, 224
DpnI GATC 1 cut(s) 517
DpnII GATC 1 cut(s) 515
DraIII CACNNNGTG 1 cut(s) 489
Eam1104I CTCTTC 2 cut(s) 381, 456
EarI CTCTTC 2 cut(s) 381, 456
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 357
Eco47I GGWCC 1 cut(s) 531
Eco57I CTGAAG 1 cut(s) 543
Eco72I CACGTG 1 cut(s) 271
Eco88I CYCGRG 1 cut(s) 143
EcoRII CCWGG 1 cut(s) 257
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 3 cut(s) 119, 133, 402
FatI CATG 3 cut(s) 115, 129, 398
FauI CCCGC 2 cut(s) 142, 190
FblI GTMKAC 1 cut(s) 139
Fnu4HI GCNGC 4 cut(s) 192, 221, 459, 498
FriOI GRGCYC 1 cut(s) 357
Fsp4HI GCNGC 4 cut(s) 192, 221, 459, 498
FspI TGCGCA 1 cut(s) 397
GlaI GCGC 1 cut(s) 397
GluI GCNGC 4 cut(s) 192, 221, 459, 498
HaeIII GGCC 2 cut(s) 263, 461
HapII CCGG 1 cut(s) 333
HhaI GCGC 1 cut(s) 398
Hin1II CATG 3 cut(s) 119, 133, 402
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HindIII AAGCTT 1 cut(s) 539
HinfI GANTC 4 cut(s) 163, 212, 341, 428
HpaII CCGG 1 cut(s) 333
HphI GGTGA 2 cut(s) 8, 335
Hpy166II GTNNAC 2 cut(s) 140, 230
Hpy188I TCNGA 3 cut(s) 156, 168, 427
Hpy188III TCNNGA 2 cut(s) 116, 434
Hpy8I GTNNAC 2 cut(s) 140, 230
HpyAV CCTTC 3 cut(s) 55, 100, 122
HpyCH4III ACNGT 4 cut(s) 172, 204, 469, 531
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 3 cut(s) 220, 402, 500
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 395, 455
HpyF3I CTNAG 3 cut(s) 187, 195, 224
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 3 cut(s) 119, 133, 402
HspAI GCGC 1 cut(s) 396
Kzo9I GATC 1 cut(s) 515
LguI GCTCTTC 1 cut(s) 456
LmnI GCTCC 1 cut(s) 386
LpnPI CCDG 8 cut(s) 173, 194, 244, 246, 263, 271, 346, 476
Lsp1109I GCAGC 2 cut(s) 232, 484
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 2 cut(s) 198, 204
MalI GATC 1 cut(s) 517
MboI GATC 1 cut(s) 515
MboII GAAGA 3 cut(s) 368, 443, 536
MhlI GDGCHC 1 cut(s) 357
MluCI AATT 1 cut(s) 507
MlyI GAGTC 2 cut(s) 157, 437
MnlI CCTC 7 cut(s) 29, 48, 119, 196, 254, 384, 430
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 1 cut(s) 333
MspR9I CCNGG 2 cut(s) 259, 334
Mva1269I GAATGC 1 cut(s) 389
MvaI CCWGG 1 cut(s) 259
MwoI GCNNNNNNNGC 3 cut(s) 191, 395, 455
NciI CCSGG 1 cut(s) 334
NcoI CCATGG 1 cut(s) 129
NdeII GATC 1 cut(s) 515
NlaIII CATG 3 cut(s) 119, 133, 402
NlaIV GGNNCC 3 cut(s) 106, 180, 372
NsbI TGCGCA 1 cut(s) 397
NspI RCATGY 1 cut(s) 402
NspV TTCGAA 1 cut(s) 339
PaeI GCATGC 1 cut(s) 402
PagI TCATGA 1 cut(s) 115
PciSI GCTCTTC 1 cut(s) 456
PctI GAATGC 1 cut(s) 389
PfeI GAWTC 2 cut(s) 212, 341
PkrI GCNGC 4 cut(s) 193, 222, 460, 499
PleI GAGTC 2 cut(s) 157, 436
PmaCI CACGTG 1 cut(s) 271
PmlI CACGTG 1 cut(s) 271
PpsI GAGTC 2 cut(s) 157, 436
Ppu21I YACGTR 1 cut(s) 271
PsiI TTATAA 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 257
PspCI CACGTG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 257
PspN4I GGNNCC 3 cut(s) 106, 180, 372
PspPI GGNCC 3 cut(s) 261, 460, 531
PstI CTGCAG 1 cut(s) 222
PstNI CAGNNNCTG 1 cut(s) 194
RseI CAYNNNNRTG 1 cut(s) 269
SapI GCTCTTC 1 cut(s) 456
SatI GCNGC 4 cut(s) 192, 221, 459, 498
Sau3AI GATC 1 cut(s) 515
Sau96I GGNCC 3 cut(s) 261, 460, 531
SchI GAGTC 2 cut(s) 157, 437
ScrFI CCNGG 2 cut(s) 259, 334
SduI GDGCHC 1 cut(s) 357
SetI ASST 8 cut(s) 21, 47, 225, 235, 260, 273, 441, 543
SfcI CTRYAG 1 cut(s) 218
SfuI TTCGAA 1 cut(s) 339
SinI GGWCC 1 cut(s) 531
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
SphI GCATGC 1 cut(s) 402
Sse9I AATT 1 cut(s) 507
SsiI CCGC 4 cut(s) 149, 183, 192, 458
StyD4I CCNGG 2 cut(s) 257, 332
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 4 cut(s) 172, 204, 469, 531
TaiI ACGT 1 cut(s) 273
TaqI TCGA 2 cut(s) 339, 505
TasI AATT 1 cut(s) 507
TauI GCSGC 2 cut(s) 194, 461
TfiI GAWTC 2 cut(s) 212, 341
TscAI CASTG 1 cut(s) 232
TseI GCWGC 2 cut(s) 220, 497
TspDTI ATGAA 2 cut(s) 17, 104
TspRI CASTG 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 531
XapI RAATTY 1 cut(s) 507
XceI RCATGY 1 cut(s) 402
XmiI GTMKAC 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.