Rw7G016290

Ycf49-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008707
Physical Location & Seq
Forward (+)
16620595 .. 16622750
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016290.1

Sequence Viewer

Length: 477 bp
ATGGCAATGAACTCCTTACTGCACCTCCCTAAACCCCATTTCCCTCCAACCTTCCCTACACACCCAAAGCCCCAAACCCAACTCTCAAAGTCCACCAGTCAATATGGTAACCCGAAAACCATGGTGGGTGGTCTACTCGGGGCGGGTCTGACCCTGTCCCTGACCGTTATGGCACCCGCCTCGGCCGCTGAGCTACCGTTACTGGATTCTCTGCAGCTCAGCGAACCTGCCAATGCCCTCTCCCTGCCCACATGGGCCATACACGTGTCCAGCGTCGTTGAATGGATTACAGCAATGGTGTTGGTGTGGCAGTATGGTGAGAAGTCCGGGTTCGAATCGTGGAAAGGGCTGGCTTGGGGTATGGTTCCCCTTCTTGGTGGAGCATTCTGTGCATGCACTTGGCATTTCTTTTATAACTCAGAGTCTCTTGAGGTAAGAAGAACAGTCGTTTTCCATGTTTTTGAATGGGATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2499

Protein Analysis

158

Amino Acids

17.38

Weight (kDa)

6.27

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2499 PF10693 79 - 145 1.4e-29 Protein of unknown function (DUF2499)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 172
AccI GTMKAC 1 cut(s) 133
AciI CCGC 3 cut(s) 143, 177, 186
AcoI YGGCCR 1 cut(s) 183
AcvI CACGTG 1 cut(s) 265
AfiI CCNNNNNNNGG 1 cut(s) 374
AflIII ACRYGT 2 cut(s) 262, 264
AgsI TTSAA 2 cut(s) 281, 464
AluBI AGCT 2 cut(s) 193, 217
AluI AGCT 2 cut(s) 193, 217
Alw26I GTCTC 1 cut(s) 429
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 2 cut(s) 183, 255
ApeKI GCWGC 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 255
AsuC2I CCSGG 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 329
AsuII TTCGAA 1 cut(s) 333
AvaI CYCGRG 1 cut(s) 137
BanI GGYRCC 1 cut(s) 172
BbrPI CACGTG 1 cut(s) 265
BbvI GCAGC 1 cut(s) 226
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BcnI CCSGG 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 212
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 2 cut(s) 186, 215
BlpI GCTNAGC 2 cut(s) 189, 218
BlsI GCNGC 2 cut(s) 187, 216
Bme1390I CCNGG 1 cut(s) 328
BmeT110I CYCGRG 1 cut(s) 137
BmgT120I GGNCC 1 cut(s) 255
BmiI GGNNCC 2 cut(s) 174, 366
BmrFI CCNGG 1 cut(s) 328
Bpu1102I GCTNAGC 2 cut(s) 189, 218
Bpu14I TTCGAA 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 328
BsaAI YACGTR 1 cut(s) 265
BsaJI CCNNGG 2 cut(s) 120, 180
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse1I ACTGG 2 cut(s) 96, 207
Bse3DI GCAATG 2 cut(s) 12, 300
BseDI CCNNGG 2 cut(s) 120, 180
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMI GCAATG 2 cut(s) 12, 300
BseMII CTCAG 3 cut(s) 180, 232, 432
BseNI ACTGG 2 cut(s) 96, 207
BseX3I CGGCCG 1 cut(s) 183
BseXI GCAGC 1 cut(s) 226
BsgI GTGCAG 1 cut(s) 5
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 2 cut(s) 185, 257
BshNI GGYRCC 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 186
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 1 cut(s) 374
BsmAI GTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 142
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 2 cut(s) 185, 257
BsoBI CYCGRG 1 cut(s) 137
Bsp119I TTCGAA 1 cut(s) 333
Bsp1720I GCTNAGC 2 cut(s) 189, 218
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 3 cut(s) 143, 177, 186
BspANI GGCC 2 cut(s) 185, 257
BspCNI CTCAG 3 cut(s) 181, 231, 431
BspLI GGNNCC 2 cut(s) 174, 366
BspMAI CTGCAG 1 cut(s) 216
BspMI ACCTGC 1 cut(s) 235
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 172
BsrDI GCAATG 2 cut(s) 12, 300
BsrI ACTGG 2 cut(s) 96, 207
BssECI CCNNGG 2 cut(s) 120, 180
BssT1I CCWWGG 1 cut(s) 120
Bst4CI ACNGT 3 cut(s) 166, 198, 445
BstAPI GCANNNNNTGC 1 cut(s) 389
BstBAI YACGTR 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 351, 394
BstDEI CTNAG 3 cut(s) 189, 218, 418
BstDSI CCRYGG 1 cut(s) 120
BstEII GGTNACC 1 cut(s) 107
BstMAI GTCTC 1 cut(s) 429
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 2 cut(s) 185, 389
BstNSI RCATGY 1 cut(s) 396
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 326
BstSFI CTRYAG 1 cut(s) 212
BstV1I GCAGC 1 cut(s) 226
BstZI CGGCCG 1 cut(s) 183
BsuRI GGCC 2 cut(s) 185, 257
BtgI CCRYGG 1 cut(s) 120
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 351, 394
Cfr13I GGNCC 1 cut(s) 255
CseI GACGC 1 cut(s) 262
CviAII CATG 4 cut(s) 121, 252, 393, 455
CviJI RGCY 7 cut(s) 70, 185, 193, 217, 257, 349, 353
CviKI_1 RGCY 7 cut(s) 70, 185, 193, 217, 257, 349, 353
DdeI CTNAG 3 cut(s) 189, 218, 418
EaeI YGGCCR 1 cut(s) 183
EagI CGGCCG 1 cut(s) 183
EclXI CGGCCG 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 120
Eco52I CGGCCG 1 cut(s) 183
Eco72I CACGTG 1 cut(s) 265
Eco88I CYCGRG 1 cut(s) 137
Eco91I GGTNACC 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 107
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 4 cut(s) 124, 255, 396, 458
FaqI GGGAC 1 cut(s) 142
FatI CATG 4 cut(s) 120, 251, 392, 454
FauI CCCGC 2 cut(s) 136, 184
FblI GTMKAC 1 cut(s) 133
Fnu4HI GCNGC 2 cut(s) 186, 215
Fsp4HI GCNGC 2 cut(s) 186, 215
GluI GCNGC 2 cut(s) 186, 215
HaeIII GGCC 2 cut(s) 185, 257
HapII CCGG 1 cut(s) 327
HgaI GACGC 1 cut(s) 262
Hin1II CATG 4 cut(s) 124, 255, 396, 458
HinfI GANTC 3 cut(s) 206, 335, 422
HpaII CCGG 1 cut(s) 327
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 2 cut(s) 93, 134
Hpy188I TCNGA 2 cut(s) 150, 421
Hpy188III TCNNGA 1 cut(s) 428
Hpy8I GTNNAC 2 cut(s) 93, 134
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 2 cut(s) 61, 380
HpyCH4III ACNGT 3 cut(s) 166, 198, 445
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 4 cut(s) 22, 214, 392, 396
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 389
HpyF3I CTNAG 3 cut(s) 189, 218, 418
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 4 cut(s) 124, 255, 396, 458
LmnI GCTCC 1 cut(s) 380
LpnPI CCDG 9 cut(s) 109, 167, 173, 188, 240, 257, 283, 335, 340
Lsp1109I GCAGC 1 cut(s) 226
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 2 cut(s) 107, 198
MboII GAAGA 1 cut(s) 450
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 5 cut(s) 35, 54, 190, 248, 424
MslI CAYNNNNRTG 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 1 cut(s) 328
Mva1269I GAATGC 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 185, 389
NciI CCSGG 1 cut(s) 328
NcoI CCATGG 1 cut(s) 120
NlaIII CATG 4 cut(s) 124, 255, 396, 458
NlaIV GGNNCC 2 cut(s) 174, 366
NmeAIII GCCGAG 1 cut(s) 161
NspI RCATGY 1 cut(s) 396
NspV TTCGAA 1 cut(s) 333
PaeI GCATGC 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 2 cut(s) 206, 335
PflFI GACNNNGTC 1 cut(s) 154
PkrI GCNGC 2 cut(s) 187, 216
PleI GAGTC 1 cut(s) 430
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 1 cut(s) 430
Ppu21I YACGTR 1 cut(s) 265
PsiI TTATAA 1 cut(s) 414
PspCI CACGTG 1 cut(s) 265
PspEI GGTNACC 1 cut(s) 107
PspN4I GGNNCC 2 cut(s) 174, 366
PspPI GGNCC 1 cut(s) 255
PstI CTGCAG 1 cut(s) 216
PsyI GACNNNGTC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 263
SatI GCNGC 2 cut(s) 186, 215
Sau96I GGNCC 1 cut(s) 255
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 1 cut(s) 328
SetI ASST 7 cut(s) 27, 53, 195, 219, 229, 267, 435
SfcI CTRYAG 1 cut(s) 212
SfuI TTCGAA 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
SphI GCATGC 1 cut(s) 396
SsiI CCGC 3 cut(s) 143, 177, 186
StyD4I CCNGG 1 cut(s) 326
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 3 cut(s) 166, 198, 445
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 333
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 2 cut(s) 206, 335
TseI GCWGC 1 cut(s) 214
TspDTI ATGAA 1 cut(s) 23
Tth111I GACNNNGTC 1 cut(s) 154
XceI RCATGY 1 cut(s) 396
XmiI GTMKAC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.