Rh7DG189900

Ycf49-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008707
Physical Location & Seq
Forward (+)
17591884 .. 17593184
1301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG189900.1

Sequence Viewer

Length: 471 bp
ATGGCAATGAACTCCTTACTGCACCTCCCTAAACCCCATTTCCCTCCAACCTTCCCTACACACCCAAAGGCCCAAACCCAACTCTCAAAGTCCACCAGTCAATATGGTAACCCGAAAACCATGGTGGGTGGTCTACTCGGGGCGGGTCTGACCCTGTCCCTGACTGTTATGGCACCCGCCTCGGCCGCTGAGCTACCGTTACTGGATTCTCTGCAGCTCAGTGAACCTGCCAATGCCCTCTCCCTGCCCACATGGGCCATACACGTGTCCAGCGTCGTTGAATGGATTACAGCAATGGTGTTGGTGTGGCAGTATGGTGAGAAGTCCGGGTTCGAATCGTGGAAAGGGCTGGCTTGGGGTATGGTATTGGTGGCTCTTCAAGCAGCCCTGACAGTTATAGGCAATGCCACAATGTGTTTTGCGGCATATCGTATTTACAGATCAACTGAAGAACGCTCCAAGAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2499

Protein Analysis

156

Amino Acids

16.87

Weight (kDa)

9.13

Isoelectric Point (pI)

32.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2499 PF10693 79 - 122 9.8e-17 Protein of unknown function (DUF2499)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 172
AccI GTMKAC 1 cut(s) 133
AciI CCGC 4 cut(s) 143, 177, 186, 422
AcoI YGGCCR 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 468
AcvI CACGTG 1 cut(s) 265
AdeI CACNNNGTG 1 cut(s) 414
AflIII ACRYGT 2 cut(s) 262, 264
AgsI TTSAA 2 cut(s) 281, 380
AluBI AGCT 3 cut(s) 193, 217, 466
AluI AGCT 3 cut(s) 193, 217, 466
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 3 cut(s) 69, 183, 255
ApeKI GCWGC 2 cut(s) 214, 383
AspS9I GGNCC 2 cut(s) 70, 255
AsuC2I CCSGG 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 329
AsuII TTCGAA 1 cut(s) 333
AvaI CYCGRG 1 cut(s) 137
BanI GGYRCC 1 cut(s) 172
BbrPI CACGTG 1 cut(s) 265
BbvI GCAGC 2 cut(s) 226, 395
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BcnI CCSGG 1 cut(s) 328
BfmI CTRYAG 1 cut(s) 212
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 4 cut(s) 186, 215, 384, 423
BlpI GCTNAGC 1 cut(s) 189
BlsI GCNGC 4 cut(s) 187, 216, 385, 424
Bme1390I CCNGG 1 cut(s) 328
BmeT110I CYCGRG 1 cut(s) 137
BmgT120I GGNCC 2 cut(s) 70, 255
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 328
Bpu1102I GCTNAGC 1 cut(s) 189
Bpu14I TTCGAA 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 328
BsaAI YACGTR 1 cut(s) 265
BsaJI CCNNGG 2 cut(s) 120, 180
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bse1I ACTGG 2 cut(s) 96, 207
Bse3DI GCAATG 3 cut(s) 12, 300, 409
BseDI CCNNGG 2 cut(s) 120, 180
BseMI GCAATG 3 cut(s) 12, 300, 409
BseMII CTCAG 2 cut(s) 180, 232
BseNI ACTGG 2 cut(s) 96, 207
BseX3I CGGCCG 1 cut(s) 183
BseXI GCAGC 2 cut(s) 226, 395
BsgI GTGCAG 1 cut(s) 5
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 3 cut(s) 71, 185, 257
BshNI GGYRCC 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 186
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 142
BsnI GGCC 3 cut(s) 71, 185, 257
BsoBI CYCGRG 1 cut(s) 137
Bsp119I TTCGAA 1 cut(s) 333
Bsp143I GATC 1 cut(s) 440
Bsp1720I GCTNAGC 1 cut(s) 189
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 4 cut(s) 143, 177, 186, 422
BspANI GGCC 3 cut(s) 71, 185, 257
BspCNI CTCAG 2 cut(s) 181, 231
BspLI GGNNCC 1 cut(s) 174
BspMAI CTGCAG 1 cut(s) 216
BspMI ACCTGC 1 cut(s) 235
BspQI GCTCTTC 1 cut(s) 381
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 172
BsrDI GCAATG 3 cut(s) 12, 300, 409
BsrI ACTGG 2 cut(s) 96, 207
BssECI CCNNGG 2 cut(s) 120, 180
BssMI GATC 1 cut(s) 440
BssT1I CCWWGG 1 cut(s) 120
Bst4CI ACNGT 3 cut(s) 166, 198, 394
Bst6I CTCTTC 1 cut(s) 381
BstBAI YACGTR 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 2 cut(s) 189, 218
BstDSI CCRYGG 1 cut(s) 120
BstEII GGTNACC 1 cut(s) 107
BstKTI GATC 1 cut(s) 443
BstMBI GATC 1 cut(s) 440
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 2 cut(s) 185, 380
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 326
BstSFI CTRYAG 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 226, 395
BstZI CGGCCG 1 cut(s) 183
BsuRI GGCC 3 cut(s) 71, 185, 257
BtgI CCRYGG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 226
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 351
Cfr13I GGNCC 2 cut(s) 70, 255
CseI GACGC 1 cut(s) 262
CviAII CATG 2 cut(s) 121, 252
DdeI CTNAG 2 cut(s) 189, 218
DpnI GATC 1 cut(s) 442
DpnII GATC 1 cut(s) 440
DraIII CACNNNGTG 1 cut(s) 414
EaeI YGGCCR 1 cut(s) 183
EagI CGGCCG 1 cut(s) 183
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
EclXI CGGCCG 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 120
Eco52I CGGCCG 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 468
Eco72I CACGTG 1 cut(s) 265
Eco88I CYCGRG 1 cut(s) 137
Eco91I GGTNACC 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 107
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 2 cut(s) 124, 255
FaiI YATR 9 cut(s) 105, 122, 170, 253, 260, 315, 362, 398, 427
FaqI GGGAC 1 cut(s) 142
FatI CATG 2 cut(s) 120, 251
FauI CCCGC 2 cut(s) 136, 184
FblI GTMKAC 1 cut(s) 133
Fnu4HI GCNGC 4 cut(s) 186, 215, 384, 423
Fsp4HI GCNGC 4 cut(s) 186, 215, 384, 423
GluI GCNGC 4 cut(s) 186, 215, 384, 423
HaeIII GGCC 3 cut(s) 71, 185, 257
HapII CCGG 1 cut(s) 327
HgaI GACGC 1 cut(s) 262
Hin1II CATG 2 cut(s) 124, 255
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 2 cut(s) 206, 335
HpaII CCGG 1 cut(s) 327
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 3 cut(s) 93, 134, 224
Hpy188I TCNGA 1 cut(s) 150
Hpy8I GTNNAC 3 cut(s) 93, 134, 224
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 3 cut(s) 166, 198, 394
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 2 cut(s) 22, 214
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 380
HpyF3I CTNAG 2 cut(s) 189, 218
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 2 cut(s) 124, 255
Kzo9I GATC 1 cut(s) 440
LguI GCTCTTC 1 cut(s) 381
LmnI GCTCC 1 cut(s) 461
Lsp1109I GCAGC 2 cut(s) 226, 395
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 2 cut(s) 107, 198
MalI GATC 1 cut(s) 442
MboI GATC 1 cut(s) 440
MboII GAAGA 2 cut(s) 368, 461
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 4 cut(s) 35, 54, 190, 248
MslI CAYNNNNRTG 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 1 cut(s) 328
MwoI GCNNNNNNNGC 2 cut(s) 185, 380
NciI CCSGG 1 cut(s) 328
NcoI CCATGG 1 cut(s) 120
NdeII GATC 1 cut(s) 440
NlaIII CATG 2 cut(s) 124, 255
NlaIV GGNNCC 1 cut(s) 174
NmeAIII GCCGAG 1 cut(s) 161
NspV TTCGAA 1 cut(s) 333
PciSI GCTCTTC 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PfeI GAWTC 2 cut(s) 206, 335
PflFI GACNNNGTC 1 cut(s) 154
PkrI GCNGC 4 cut(s) 187, 216, 385, 424
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
Ppu21I YACGTR 1 cut(s) 265
PspCI CACGTG 1 cut(s) 265
PspEI GGTNACC 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 2 cut(s) 70, 255
PstI CTGCAG 1 cut(s) 216
PsyI GACNNNGTC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 263
SapI GCTCTTC 1 cut(s) 381
SatI GCNGC 4 cut(s) 186, 215, 384, 423
Sau3AI GATC 1 cut(s) 440
Sau96I GGNCC 2 cut(s) 70, 255
ScrFI CCNGG 1 cut(s) 328
SetI ASST 7 cut(s) 27, 53, 195, 219, 229, 267, 468
SfcI CTRYAG 1 cut(s) 212
SfuI TTCGAA 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 263
SsiI CCGC 4 cut(s) 143, 177, 186, 422
StyD4I CCNGG 1 cut(s) 326
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 3 cut(s) 166, 198, 394
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 333
TauI GCSGC 2 cut(s) 188, 425
TfiI GAWTC 2 cut(s) 206, 335
TscAI CASTG 1 cut(s) 226
TseI GCWGC 2 cut(s) 214, 383
TspDTI ATGAA 1 cut(s) 23
TspRI CASTG 1 cut(s) 226
Tth111I GACNNNGTC 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.