RLG00000003724

Ycf49-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008707
Physical Location & Seq
Reverse (-)
52090797 .. 52092136
1340 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003724

Sequence Viewer

Length: 540 bp
ATGGCAATGAACTCCTTACTGCACCTCCCTAAACCCCATTTCCCTCCAACCTTCCCTACACACCCAAAGCCCCAAACCCAACTCTCAAAGTCCACCAGTCAATATGGTAACCCAAAAATCATGGTGGGTAGTCTACTCGGGGCGGGTCTGACCATAACCCTGACCGTTATGGCACCCGCCTCGGCCGCTGAGCTACCGTTACTGGATTCTCTGCAGCTCAGTGAACCTGCCAATGCCCTCTCCCTGCCCACATGGGCCATACACGTGTCCAGTGTGGTTGAATGGATTACAGCAATGGTGTTGGTGTGGCAGTATGGTGAGAAGTCCGGGTTCGAATCGTGGAAAGGGCTGGCTTGGGGTATGGTTCCCCTTCTTGGTGGAGCATTCTGTGCATGCACTTGGCATTTCTTTTATAACTCAGAGTCTCTTGAGGTATTGGTGGCTCTTCAAGCAGCCCTGACAGTTATAGGCAATGCCACAATGTGTTTTGCGGCATATCGTATTTACAGATCAACTGAAGAACGCTCCAAGAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2499

Protein Analysis

180

Amino Acids

19.53

Weight (kDa)

7.76

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2499 PF10693 79 - 166 1.3e-39 Protein of unknown function (DUF2499)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 172
AccI GTMKAC 1 cut(s) 133
AciI CCGC 4 cut(s) 143, 177, 186, 491
AcoI YGGCCR 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 537
AcvI CACGTG 1 cut(s) 265
AdeI CACNNNGTG 1 cut(s) 483
AfiI CCNNNNNNNGG 1 cut(s) 374
AflIII ACRYGT 2 cut(s) 262, 264
AgsI TTSAA 2 cut(s) 281, 449
AluBI AGCT 3 cut(s) 193, 217, 535
AluI AGCT 3 cut(s) 193, 217, 535
Alw26I GTCTC 1 cut(s) 429
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 2 cut(s) 183, 255
ApeKI GCWGC 2 cut(s) 214, 452
AspS9I GGNCC 1 cut(s) 255
AsuC2I CCSGG 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 329
AsuII TTCGAA 1 cut(s) 333
AvaI CYCGRG 1 cut(s) 137
BanI GGYRCC 1 cut(s) 172
BbrPI CACGTG 1 cut(s) 265
BbvI GCAGC 2 cut(s) 226, 464
BcgI CGANNNNNNTGC 2 cut(s) 162, 196
BcnI CCSGG 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 212
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 4 cut(s) 186, 215, 453, 492
BlpI GCTNAGC 1 cut(s) 189
BlsI GCNGC 4 cut(s) 187, 216, 454, 493
Bme1390I CCNGG 1 cut(s) 328
BmeT110I CYCGRG 1 cut(s) 137
BmgT120I GGNCC 1 cut(s) 255
BmiI GGNNCC 2 cut(s) 174, 366
BmrFI CCNGG 1 cut(s) 328
Bpu1102I GCTNAGC 1 cut(s) 189
Bpu14I TTCGAA 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 328
BsaAI YACGTR 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 180
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse1I ACTGG 3 cut(s) 96, 207, 270
Bse3DI GCAATG 3 cut(s) 12, 300, 478
BseDI CCNNGG 1 cut(s) 180
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMI GCAATG 3 cut(s) 12, 300, 478
BseMII CTCAG 3 cut(s) 180, 232, 432
BseNI ACTGG 3 cut(s) 96, 207, 270
BseX3I CGGCCG 1 cut(s) 183
BseXI GCAGC 2 cut(s) 226, 464
BsgI GTGCAG 1 cut(s) 5
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 2 cut(s) 185, 257
BshNI GGYRCC 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 186
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 327
BslI CCNNNNNNNGG 1 cut(s) 374
BsmAI GTCTC 1 cut(s) 429
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 2 cut(s) 185, 257
BsoBI CYCGRG 1 cut(s) 137
Bsp119I TTCGAA 1 cut(s) 333
Bsp143I GATC 1 cut(s) 509
Bsp1720I GCTNAGC 1 cut(s) 189
BspACI CCGC 4 cut(s) 143, 177, 186, 491
BspANI GGCC 2 cut(s) 185, 257
BspCNI CTCAG 3 cut(s) 181, 231, 431
BspLI GGNNCC 2 cut(s) 174, 366
BspMAI CTGCAG 1 cut(s) 216
BspMI ACCTGC 1 cut(s) 235
BspQI GCTCTTC 1 cut(s) 450
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 172
BsrDI GCAATG 3 cut(s) 12, 300, 478
BsrI ACTGG 3 cut(s) 96, 207, 270
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 1 cut(s) 509
Bst4CI ACNGT 3 cut(s) 166, 198, 463
Bst6I CTCTTC 1 cut(s) 450
BstAPI GCANNNNNTGC 1 cut(s) 389
BstBAI YACGTR 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 351, 394
BstDEI CTNAG 3 cut(s) 189, 218, 418
BstEII GGTNACC 1 cut(s) 107
BstKTI GATC 1 cut(s) 512
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 1 cut(s) 509
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 3 cut(s) 185, 389, 449
BstNSI RCATGY 1 cut(s) 396
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 326
BstSFI CTRYAG 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 226, 464
BstZI CGGCCG 1 cut(s) 183
BsuRI GGCC 2 cut(s) 185, 257
BtsIMutI CAGTG 2 cut(s) 226, 277
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 351, 394
Cfr13I GGNCC 1 cut(s) 255
CviAII CATG 3 cut(s) 121, 252, 393
DdeI CTNAG 3 cut(s) 189, 218, 418
DpnI GATC 1 cut(s) 511
DpnII GATC 1 cut(s) 509
DraIII CACNNNGTG 1 cut(s) 483
EaeI YGGCCR 1 cut(s) 183
EagI CGGCCG 1 cut(s) 183
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
EclXI CGGCCG 1 cut(s) 183
Eco52I CGGCCG 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 537
Eco72I CACGTG 1 cut(s) 265
Eco88I CYCGRG 1 cut(s) 137
Eco91I GGTNACC 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 107
FaeI CATG 3 cut(s) 124, 255, 396
FatI CATG 3 cut(s) 120, 251, 392
FauI CCCGC 2 cut(s) 136, 184
FblI GTMKAC 1 cut(s) 133
Fnu4HI GCNGC 4 cut(s) 186, 215, 453, 492
Fsp4HI GCNGC 4 cut(s) 186, 215, 453, 492
GluI GCNGC 4 cut(s) 186, 215, 453, 492
HaeIII GGCC 2 cut(s) 185, 257
HapII CCGG 1 cut(s) 327
Hin1II CATG 3 cut(s) 124, 255, 396
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 3 cut(s) 206, 335, 422
HpaII CCGG 1 cut(s) 327
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 3 cut(s) 93, 134, 224
Hpy188I TCNGA 2 cut(s) 150, 421
Hpy188III TCNNGA 1 cut(s) 428
Hpy8I GTNNAC 3 cut(s) 93, 134, 224
HpyAV CCTTC 2 cut(s) 61, 380
HpyCH4III ACNGT 3 cut(s) 166, 198, 463
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 4 cut(s) 22, 214, 392, 396
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 389, 449
HpyF3I CTNAG 3 cut(s) 189, 218, 418
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 3 cut(s) 124, 255, 396
Kzo9I GATC 1 cut(s) 509
LguI GCTCTTC 1 cut(s) 450
LmnI GCTCC 2 cut(s) 380, 530
LpnPI CCDG 9 cut(s) 109, 173, 188, 240, 257, 283, 335, 340, 470
Lsp1109I GCAGC 2 cut(s) 226, 464
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 2 cut(s) 107, 198
MalI GATC 1 cut(s) 511
MboI GATC 1 cut(s) 509
MboII GAAGA 2 cut(s) 437, 530
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 5 cut(s) 35, 54, 190, 248, 424
MslI CAYNNNNRTG 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 1 cut(s) 328
Mva1269I GAATGC 1 cut(s) 383
MwoI GCNNNNNNNGC 3 cut(s) 185, 389, 449
NciI CCSGG 1 cut(s) 328
NdeII GATC 1 cut(s) 509
NlaIII CATG 3 cut(s) 124, 255, 396
NlaIV GGNNCC 2 cut(s) 174, 366
NmeAIII GCCGAG 1 cut(s) 161
NspI RCATGY 1 cut(s) 396
NspV TTCGAA 1 cut(s) 333
PaeI GCATGC 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 450
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 2 cut(s) 206, 335
PkrI GCNGC 4 cut(s) 187, 216, 454, 493
PleI GAGTC 1 cut(s) 430
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 1 cut(s) 430
Ppu21I YACGTR 1 cut(s) 265
PsiI TTATAA 1 cut(s) 414
PspCI CACGTG 1 cut(s) 265
PspEI GGTNACC 1 cut(s) 107
PspN4I GGNNCC 2 cut(s) 174, 366
PspPI GGNCC 1 cut(s) 255
PstI CTGCAG 1 cut(s) 216
RseI CAYNNNNRTG 1 cut(s) 263
SapI GCTCTTC 1 cut(s) 450
SatI GCNGC 4 cut(s) 186, 215, 453, 492
Sau3AI GATC 1 cut(s) 509
Sau96I GGNCC 1 cut(s) 255
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 1 cut(s) 328
SetI ASST 8 cut(s) 27, 53, 195, 219, 229, 267, 435, 537
SfcI CTRYAG 1 cut(s) 212
SfuI TTCGAA 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
SphI GCATGC 1 cut(s) 396
SsiI CCGC 4 cut(s) 143, 177, 186, 491
StyD4I CCNGG 1 cut(s) 326
TaaI ACNGT 3 cut(s) 166, 198, 463
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 333
TauI GCSGC 2 cut(s) 188, 494
TfiI GAWTC 2 cut(s) 206, 335
TscAI CASTG 2 cut(s) 226, 277
TseI GCWGC 2 cut(s) 214, 452
TspDTI ATGAA 1 cut(s) 23
TspRI CASTG 2 cut(s) 226, 277
XceI RCATGY 1 cut(s) 396
XmiI GTMKAC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.