MD06G1068000.v1.1

Ycf49-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008707
Physical Location & Seq
Reverse (-)
15390181 .. 15392773
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1068000.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGCAATGCACTCCTTACTTCACCTTCCAAAACCCACTTTCCCGCGCAAATCCCTTTTCCTTCCGCGCCCAAAACCTCAAACCCAACCGCCAAATTCCCACCATTACCCCAAAATCTTGGTGGGTGTACTCGGGGCTGGTCTGACCCTGGCCTTCGTCGGACCCGCCTCAGCTTCTGAGCTGCTGCCCTTGAATGAACCCTCCAATGCCCTCTCGTTGCCCACCTGGGCCATTCACGTTTCCAGCGTCGTTGAATGGGTTACAGCAATGGCATTGGTGTGGCAGTACGGGGAGAAGTCCGGGTTCGAATCTTGGAAAGGGCTGTCTTGGGGGATGGTTCCCTTGCTTGGTGGAGCATTATGTGCATGCACATGGCATTTCTTTTATAACTCTGATTCTCTTGAAGTACTGGTTGCTCTTCAAGCAGCCCTGACAGTAATAGGGAATGCCACAATGTGTTTTGCTGCATATCGTATTTACAGATCATCCGAAGAACACTCCAAGAATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2499

Protein Analysis

171

Amino Acids

18.62

Weight (kDa)

8.48

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2499 PF10693 70 - 157 2.9e-39 Protein of unknown function (DUF2499)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 387
AccII CGCG 2 cut(s) 46, 67
AciI CCGC 4 cut(s) 44, 65, 89, 165
AcsI RAATTY 1 cut(s) 94
AdeI CACNNNGTG 1 cut(s) 456
AfaI GTAC 3 cut(s) 129, 287, 408
AfiI CCNNNNNNNGG 1 cut(s) 347
AgsI TTSAA 4 cut(s) 193, 254, 404, 422
AjnI CCWGG 2 cut(s) 147, 224
AluBI AGCT 2 cut(s) 173, 181
AluI AGCT 2 cut(s) 173, 181
AlwNI CAGNNNCTG 1 cut(s) 176
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 2 cut(s) 150, 228
ApeKI GCWGC 4 cut(s) 181, 184, 425, 464
ApoI RAATTY 1 cut(s) 94
AspLEI GCGC 2 cut(s) 48, 69
AspS9I GGNCC 2 cut(s) 161, 228
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 1 cut(s) 14
AsuII TTCGAA 1 cut(s) 306
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 1 cut(s) 161
BbvCI CCTCAGC 1 cut(s) 169
BbvI GCAGC 4 cut(s) 168, 171, 437, 451
BccI CCATC 1 cut(s) 328
BciT130I CCWGG 2 cut(s) 149, 226
BcnI CCSGG 1 cut(s) 301
BisI GCNGC 4 cut(s) 182, 185, 426, 465
BlsI GCNGC 4 cut(s) 183, 186, 427, 466
BmcAI AGTACT 1 cut(s) 408
Bme1390I CCNGG 3 cut(s) 149, 226, 301
Bme18I GGWCC 1 cut(s) 161
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 2 cut(s) 161, 228
BmiI GGNNCC 2 cut(s) 163, 339
BmrFI CCNGG 3 cut(s) 149, 226, 301
Bpu10I CCTNAGC 1 cut(s) 169
Bpu14I TTCGAA 1 cut(s) 306
BpuMI CCSGG 1 cut(s) 301
BsaJI CCNNGG 2 cut(s) 147, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 414
Bse3DI GCAATG 2 cut(s) 12, 273
BseBI CCWGG 2 cut(s) 149, 226
BseDI CCNNGG 2 cut(s) 147, 225
BseGI GGATG 2 cut(s) 339, 485
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMI GCAATG 2 cut(s) 12, 273
BseMII CTCAG 2 cut(s) 168, 183
BseNI ACTGG 1 cut(s) 414
BseXI GCAGC 4 cut(s) 168, 171, 437, 451
Bsh1236I CGCG 2 cut(s) 46, 67
BshFI GGCC 2 cut(s) 152, 230
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 1 cut(s) 300
BslI CCNNNNNNNGG 1 cut(s) 347
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 2 cut(s) 152, 230
BsoBI CYCGRG 1 cut(s) 131
Bsp119I TTCGAA 1 cut(s) 306
Bsp143I GATC 1 cut(s) 482
BspACI CCGC 4 cut(s) 44, 65, 89, 165
BspANI GGCC 2 cut(s) 152, 230
BspCNI CTCAG 2 cut(s) 169, 182
BspFNI CGCG 2 cut(s) 46, 67
BspLI GGNNCC 2 cut(s) 163, 339
BspQI GCTCTTC 1 cut(s) 423
BspT104I TTCGAA 1 cut(s) 306
BsrDI GCAATG 2 cut(s) 12, 273
BsrI ACTGG 1 cut(s) 414
BssECI CCNNGG 2 cut(s) 147, 225
BssMI GATC 1 cut(s) 482
Bst2UI CCWGG 2 cut(s) 149, 226
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 362
BstBI TTCGAA 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 367
BstDEI CTNAG 2 cut(s) 169, 177
BstF5I GGATG 2 cut(s) 339, 485
BstFNI CGCG 2 cut(s) 46, 67
BstHHI GCGC 2 cut(s) 48, 69
BstKTI GATC 1 cut(s) 485
BstMBI GATC 1 cut(s) 482
BstMWI GCNNNNNNNGC 2 cut(s) 362, 422
BstNI CCWGG 2 cut(s) 149, 226
BstNSI RCATGY 1 cut(s) 369
BstSCI CCNGG 3 cut(s) 147, 224, 299
BstUI CGCG 2 cut(s) 46, 67
BstV1I GCAGC 4 cut(s) 168, 171, 437, 451
BstXI CCANNNNNNTGG 1 cut(s) 118
BsuRI GGCC 2 cut(s) 152, 230
BtsCI GGATG 2 cut(s) 339, 485
Cac8I GCNNGC 1 cut(s) 367
CaiI CAGNNNCTG 1 cut(s) 176
CfoI GCGC 2 cut(s) 48, 69
Cfr13I GGNCC 2 cut(s) 161, 228
CseI GACGC 1 cut(s) 235
Csp6I GTAC 3 cut(s) 128, 286, 407
CviAII CATG 2 cut(s) 366, 372
CviJI RGCY 7 cut(s) 137, 152, 173, 181, 230, 322, 428
CviKI_1 RGCY 7 cut(s) 137, 152, 173, 181, 230, 322, 428
CviQI GTAC 3 cut(s) 128, 286, 407
DdeI CTNAG 2 cut(s) 169, 177
DpnI GATC 1 cut(s) 484
DpnII GATC 1 cut(s) 482
DraIII CACNNNGTG 1 cut(s) 456
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
Eco47I GGWCC 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 131
EcoRII CCWGG 2 cut(s) 147, 224
FaeI CATG 2 cut(s) 369, 375
FaiI YATR 5 cut(s) 361, 367, 373, 387, 469
FatI CATG 2 cut(s) 365, 371
FauI CCCGC 2 cut(s) 51, 172
Fnu4HI GCNGC 4 cut(s) 182, 185, 426, 465
FokI GGATG 2 cut(s) 346, 472
Fsp4HI GCNGC 4 cut(s) 182, 185, 426, 465
GlaI GCGC 2 cut(s) 47, 68
GluI GCNGC 4 cut(s) 182, 185, 426, 465
HaeIII GGCC 2 cut(s) 152, 230
HapII CCGG 1 cut(s) 300
HgaI GACGC 1 cut(s) 235
HhaI GCGC 2 cut(s) 48, 69
Hin1II CATG 2 cut(s) 369, 375
Hin6I GCGC 2 cut(s) 46, 67
HinP1I GCGC 2 cut(s) 46, 67
HinfI GANTC 3 cut(s) 308, 395, 505
HpaII CCGG 1 cut(s) 300
HphI GGTGA 1 cut(s) 14
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 5 cut(s) 144, 161, 178, 394, 490
Hpy188III TCNNGA 1 cut(s) 401
Hpy8I GTNNAC 1 cut(s) 128
Hpy99I CGWCG 2 cut(s) 161, 251
HpyAV CCTTC 3 cut(s) 35, 71, 163
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 4 cut(s) 10, 365, 369, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 422
HpyF3I CTNAG 2 cut(s) 169, 177
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 2 cut(s) 369, 375
HspAI GCGC 2 cut(s) 46, 67
Kzo9I GATC 1 cut(s) 482
LguI GCTCTTC 1 cut(s) 423
LmnI GCTCC 1 cut(s) 353
LpnPI CCDG 9 cut(s) 123, 134, 161, 211, 238, 256, 313, 395, 443
Lsp1109I GCAGC 4 cut(s) 168, 171, 437, 451
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 259
MalI GATC 1 cut(s) 484
MboI GATC 1 cut(s) 482
MboII GAAGA 2 cut(s) 410, 503
MluCI AATT 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 4 cut(s) 87, 178, 211, 221
MslI CAYNNNNRTG 2 cut(s) 277, 370
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 3 cut(s) 149, 226, 301
Mva1269I GAATGC 1 cut(s) 451
MvaI CCWGG 2 cut(s) 149, 226
MvnI CGCG 2 cut(s) 46, 67
MwoI GCNNNNNNNGC 2 cut(s) 362, 422
NciI CCSGG 1 cut(s) 301
NdeII GATC 1 cut(s) 482
NlaIII CATG 2 cut(s) 369, 375
NlaIV GGNNCC 2 cut(s) 163, 339
NspI RCATGY 1 cut(s) 369
NspV TTCGAA 1 cut(s) 306
PaeI GCATGC 1 cut(s) 369
PciSI GCTCTTC 1 cut(s) 423
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 1 cut(s) 451
PfeI GAWTC 3 cut(s) 308, 395, 505
PkrI GCNGC 4 cut(s) 183, 186, 427, 466
PsiI TTATAA 1 cut(s) 387
Psp6I CCWGG 2 cut(s) 147, 224
PspGI CCWGG 2 cut(s) 147, 224
PspN4I GGNNCC 2 cut(s) 163, 339
PspPI GGNCC 2 cut(s) 161, 228
PstNI CAGNNNCTG 1 cut(s) 176
RsaI GTAC 3 cut(s) 129, 287, 408
RsaNI GTAC 3 cut(s) 128, 286, 407
RseI CAYNNNNRTG 2 cut(s) 277, 370
SapI GCTCTTC 1 cut(s) 423
SatI GCNGC 4 cut(s) 182, 185, 426, 465
Sau3AI GATC 1 cut(s) 482
Sau96I GGNCC 2 cut(s) 161, 228
ScaI AGTACT 1 cut(s) 408
ScrFI CCNGG 3 cut(s) 149, 226, 301
SetI ASST 6 cut(s) 27, 79, 175, 183, 227, 240
SfuI TTCGAA 1 cut(s) 306
SinI GGWCC 1 cut(s) 161
SmiMI CAYNNNNRTG 2 cut(s) 277, 370
SphI GCATGC 1 cut(s) 369
Sse9I AATT 1 cut(s) 94
SsiI CCGC 4 cut(s) 44, 65, 89, 165
StyD4I CCNGG 3 cut(s) 147, 224, 299
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 1 cut(s) 240
TaqI TCGA 1 cut(s) 306
TasI AATT 1 cut(s) 94
TatI WGTACW 2 cut(s) 127, 406
TfiI GAWTC 3 cut(s) 308, 395, 505
TseI GCWGC 4 cut(s) 181, 184, 425, 464
TspDTI ATGAA 1 cut(s) 210
VpaK11BI GGWCC 1 cut(s) 161
XapI RAATTY 1 cut(s) 94
XceI RCATGY 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
ZrmI AGTACT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.