Prupe.5G082400_v2.0.a1

Ycf49-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0008707
Physical Location & Seq
Reverse (-)
9580360 .. 9582984
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G082400.3

Sequence Viewer

Length: 549 bp
ATGGCAATGAACTCCTTACTGTACCTCCCAAAACTCCCTTTTCCTCACAAACCCCTTTCCCCTCCAACGTTCCGTACACACCCAAAAGCTCTGACCCGACCCGCAAAGCCCCTCAGTCAAAATGGTACCCTGGGTTTTCTCGGAGCGGGTCTGACCCTGACCCTGACCCTGACCCTTATTGGACCCGCCTCAGCTGCTGAGTTACCGTTGTGGGGATCCTTGCAGCTCAGTGAACCCTCCAATGCCCTCTCTTTGCCCACCTGGGCCATACACGTTTCTAGTGTCGTTGAATGGATTACAGCAATGGCTTTGGTGTGGCAGTATGGGGAGAAGTCTGGGTTTGAATCTTGGAAAGGGCTGTCTTGGGGTATGGTTCCCCTGCTCGGTGGAGCATTGTGTGCATGCACATGGCATTTCTTTTATAACTCTGAGTCTCTTGAGGTCCTGGTGGCTCTTCAAGCAGCCCTGACAGTTATCGGCAATGCTACAATGTGTTTTGCTGCATTTCGTATTTACAGATCATCTGAAGAACGCTCCAAGAATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2499

Protein Analysis

183

Amino Acids

19.79

Weight (kDa)

8.86

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 423
Acc65I GGTACC 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 125
AccBSI CCGCTC 1 cut(s) 146
AciI CCGC 3 cut(s) 102, 146, 186
AclI AACGTT 1 cut(s) 68
AclWI GGATC 2 cut(s) 210, 223
AcuI CTGAAG 1 cut(s) 546
AfaI GTAC 3 cut(s) 23, 76, 127
AfiI CCNNNNNNNGG 2 cut(s) 212, 383
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 3 cut(s) 290, 344, 458
AjnI CCWGG 3 cut(s) 129, 260, 444
AluBI AGCT 3 cut(s) 89, 194, 226
AluI AGCT 3 cut(s) 89, 194, 226
Alw26I GTCTC 1 cut(s) 438
AlwI GGATC 2 cut(s) 210, 223
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 1 cut(s) 264
ApeKI GCWGC 4 cut(s) 194, 223, 461, 500
Asp718I GGTACC 1 cut(s) 125
AspS9I GGNCC 3 cut(s) 182, 264, 442
AvaII GGWCC 2 cut(s) 182, 442
BamHI GGATCC 1 cut(s) 215
BanI GGYRCC 1 cut(s) 125
BbvCI CCTCAGC 1 cut(s) 190
BbvI GCAGC 4 cut(s) 181, 235, 473, 487
BciT130I CCWGG 3 cut(s) 131, 262, 446
BcoDI GTCTC 1 cut(s) 438
BfaI CTAG 1 cut(s) 279
BisI GCNGC 4 cut(s) 195, 224, 462, 501
BlsI GCNGC 4 cut(s) 196, 225, 463, 502
Bme1390I CCNGG 3 cut(s) 131, 262, 446
Bme18I GGWCC 2 cut(s) 182, 442
BmgT120I GGNCC 3 cut(s) 182, 264, 442
BmiI GGNNCC 4 cut(s) 127, 184, 217, 375
BmrFI CCNGG 3 cut(s) 131, 262, 446
Bpu10I CCTNAGC 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 458
BsaJI CCNNGG 3 cut(s) 129, 130, 261
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 383
Bse3DI GCAATG 3 cut(s) 12, 309, 487
BseBI CCWGG 3 cut(s) 131, 262, 446
BseDI CCNNGG 3 cut(s) 129, 130, 261
BseLI CCNNNNNNNGG 2 cut(s) 212, 383
BseMI GCAATG 3 cut(s) 12, 309, 487
BseMII CTCAG 5 cut(s) 127, 189, 204, 241, 420
BseXI GCAGC 4 cut(s) 181, 235, 473, 487
BshFI GGCC 1 cut(s) 266
BshNI GGYRCC 1 cut(s) 125
BslI CCNNNNNNNGG 2 cut(s) 212, 383
BsmAI GTCTC 1 cut(s) 438
BsnI GGCC 1 cut(s) 266
Bsp143I GATC 2 cut(s) 215, 518
BspACI CCGC 3 cut(s) 102, 146, 186
BspANI GGCC 1 cut(s) 266
BspCNI CTCAG 5 cut(s) 126, 190, 203, 240, 421
BspLI GGNNCC 4 cut(s) 127, 184, 217, 375
BspPI GGATC 2 cut(s) 210, 223
BspQI GCTCTTC 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 125
BsrBI CCGCTC 1 cut(s) 146
BsrDI GCAATG 3 cut(s) 12, 309, 487
BssECI CCNNGG 3 cut(s) 129, 130, 261
BssMI GATC 2 cut(s) 215, 518
Bst2UI CCWGG 3 cut(s) 131, 262, 446
Bst4CI ACNGT 3 cut(s) 21, 207, 472
Bst6I CTCTTC 1 cut(s) 459
BstAPI GCANNNNNTGC 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 5 cut(s) 113, 190, 198, 227, 429
BstKTI GATC 2 cut(s) 218, 521
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 2 cut(s) 215, 518
BstMWI GCNNNNNNNGC 3 cut(s) 194, 398, 458
BstNI CCWGG 3 cut(s) 131, 262, 446
BstNSI RCATGY 1 cut(s) 405
BstSCI CCNGG 3 cut(s) 129, 260, 444
BstV1I GCAGC 4 cut(s) 181, 235, 473, 487
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
BsuRI GGCC 1 cut(s) 266
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 403
CaiI CAGNNNCTG 1 cut(s) 197
Cfr13I GGNCC 3 cut(s) 182, 264, 442
Csp6I GTAC 3 cut(s) 22, 75, 126
CviAII CATG 2 cut(s) 402, 408
CviJI RGCY 9 cut(s) 89, 109, 194, 226, 266, 308, 358, 452, 464
CviKI_1 RGCY 9 cut(s) 89, 109, 194, 226, 266, 308, 358, 452, 464
CviQI GTAC 3 cut(s) 22, 75, 126
DdeI CTNAG 5 cut(s) 113, 190, 198, 227, 429
DpnI GATC 2 cut(s) 217, 520
DpnII GATC 2 cut(s) 215, 518
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Eco47I GGWCC 2 cut(s) 182, 442
Eco57I CTGAAG 1 cut(s) 546
EcoO109I RGGNCCY 1 cut(s) 442
EcoRII CCWGG 3 cut(s) 129, 260, 444
FaeI CATG 2 cut(s) 405, 411
FaiI YATR 6 cut(s) 269, 324, 371, 403, 409, 423
FatI CATG 2 cut(s) 401, 407
FauI CCCGC 3 cut(s) 109, 139, 193
Fnu4HI GCNGC 4 cut(s) 195, 224, 462, 501
Fsp4HI GCNGC 4 cut(s) 195, 224, 462, 501
FspBI CTAG 1 cut(s) 279
GluI GCNGC 4 cut(s) 195, 224, 462, 501
HaeIII GGCC 1 cut(s) 266
Hin1II CATG 2 cut(s) 405, 411
HinfI GANTC 3 cut(s) 344, 431, 541
Hpy166II GTNNAC 2 cut(s) 77, 233
Hpy188I TCNGA 5 cut(s) 93, 143, 153, 430, 526
Hpy188III TCNNGA 1 cut(s) 437
Hpy8I GTNNAC 2 cut(s) 77, 233
HpyCH4III ACNGT 3 cut(s) 21, 207, 472
HpyCH4IV ACGT 2 cut(s) 68, 273
HpyCH4V TGCA 4 cut(s) 223, 401, 405, 503
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 398, 458
HpyF3I CTNAG 5 cut(s) 113, 190, 198, 227, 429
HpySE526I ACGT 2 cut(s) 68, 273
Hsp92II CATG 2 cut(s) 405, 411
KpnI GGTACC 1 cut(s) 129
Kzo9I GATC 2 cut(s) 215, 518
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 3 cut(s) 143, 389, 539
Lsp1109I GCAGC 4 cut(s) 181, 235, 473, 487
MaeI CTAG 1 cut(s) 279
MaeII ACGT 2 cut(s) 68, 273
MaeIII GTNAC 1 cut(s) 201
MalI GATC 2 cut(s) 217, 520
MbiI CCGCTC 1 cut(s) 146
MboI GATC 2 cut(s) 215, 518
MboII GAAGA 2 cut(s) 446, 539
MflI RGATCY 1 cut(s) 215
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 8 cut(s) 35, 54, 72, 122, 199, 247, 257, 433
MslI CAYNNNNRTG 1 cut(s) 406
MspA1I CMGCKG 1 cut(s) 194
MspR9I CCNGG 3 cut(s) 131, 262, 446
MvaI CCWGG 3 cut(s) 131, 262, 446
MwoI GCNNNNNNNGC 3 cut(s) 194, 398, 458
NdeII GATC 2 cut(s) 215, 518
NlaIII CATG 2 cut(s) 405, 411
NlaIV GGNNCC 4 cut(s) 127, 184, 217, 375
NspI RCATGY 1 cut(s) 405
PaeI GCATGC 1 cut(s) 405
PasI CCCWGGG 1 cut(s) 130
PciSI GCTCTTC 1 cut(s) 459
PfeI GAWTC 2 cut(s) 344, 541
PkrI GCNGC 4 cut(s) 196, 225, 463, 502
PleI GAGTC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 439
PpuMI RGGWCCY 1 cut(s) 442
PsiI TTATAA 1 cut(s) 423
Psp1406I AACGTT 1 cut(s) 68
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 3 cut(s) 129, 260, 444
PspGI CCWGG 3 cut(s) 129, 260, 444
PspN4I GGNNCC 4 cut(s) 127, 184, 217, 375
PspPI GGNCC 3 cut(s) 182, 264, 442
PspPPI RGGWCCY 1 cut(s) 442
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 1 cut(s) 215
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 3 cut(s) 23, 76, 127
RsaNI GTAC 3 cut(s) 22, 75, 126
RseI CAYNNNNRTG 1 cut(s) 406
SapI GCTCTTC 1 cut(s) 459
SatI GCNGC 4 cut(s) 195, 224, 462, 501
Sau3AI GATC 2 cut(s) 215, 518
Sau96I GGNCC 3 cut(s) 182, 264, 442
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 3 cut(s) 131, 262, 446
SetI ASST 8 cut(s) 27, 71, 91, 196, 228, 263, 276, 444
SinI GGWCC 2 cut(s) 182, 442
SmiMI CAYNNNNRTG 1 cut(s) 406
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SphI GCATGC 1 cut(s) 405
SsiI CCGC 3 cut(s) 102, 146, 186
SspMI CTAG 1 cut(s) 279
StyD4I CCNGG 3 cut(s) 129, 260, 444
TaaI ACNGT 3 cut(s) 21, 207, 472
TaiI ACGT 2 cut(s) 71, 276
TfiI GAWTC 2 cut(s) 344, 541
TscAI CASTG 1 cut(s) 235
TseI GCWGC 4 cut(s) 194, 223, 461, 500
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 2 cut(s) 182, 442
XceI RCATGY 1 cut(s) 405
XspI CTAG 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.