AT4G24290
ERF Family

membrane-attack complex / perforin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007444
Physical Location & Seq
Forward (+)
12594045 .. 12598097
4053 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G24290.2

Sequence Viewer

Length: 1053 bp
ATGGCTCTTCGGCTACCAGCTTCTAAAGCAGCTGAAGTTGCGATTGGATCTATTGGCTGTGGTTATGATCTAGCTATTGATTTACGGTTGAAGTATTGCAAAGGAGGTTCCAAAGATTCTAGATTACTTGACATTAAAGAAGGTGATGATAATTGTGAGATTGTTTTACCTGGTGGAATCTCCATTCCTAATGTTTCCAAGTCTATCAAATGCGATAAAGGGGAGCGTATGCGGTTTAGGTCCGATATTCTTCCTTTCCAACAGATGGCAGAGCAGTTCAACCAGGAACTATCTTTGGCTGGGAAAATCCCGTCAGGTCTCTTCAATGCCATGTTTGAATTCTCGAGCTGTTGGCAGAAAGACGCAGCCTACACCAAAAACCTTGCTTTTGATGGGGTTTTCATCTCATTATACTCGGTGGCTTTGGACAAATCTCAAGTGTTACTCCGTGAGCATGTTAAGCAGGCTGTTCCATCAACATGGGACCCTGCTGCATTGGCAAGGTTTATAGATATTTATGGGACGCATATAATTGTTAGTGTTAAGATGGGAGGAAAAGATGTGATTTATGCAAAACAACAACACTCCTCAAAACTTCAGCCTGAGGATCTGCAGAAAAGGTTAAAAGAGGTGGCAGATAAGAGGTTCGTGGAAGCTAGCGTAGTGCATAATACGGGTTCAGAAAGAGTACAAGCAAGTAGTAAGGTGGAAACAAAGGAGCAACGCCTGAGATTTGCAGATACCAGTTCTTTGGGCTCTTATGCAAATAAGGAGGACTATGTCTTCATGTGCAAGAGGCGAGGTGGAAACGATAACAGAAATCTAATGCATAATGAATGGCTGCAAACAGTTCAGATGGAGCCTGATGTTATCTCCATGTCTTTTATTCCAATTACGTCTTTGCTTAATGGAGTTCCAGGAAGTGGATTCTTAAGCCATGCCATAAATCTGTATCTAAGATGTAAGCCTATAAATCATTTGCGCTTTGTGCATTTCTTTCTGGAGTTATTCTTCATACTAGTGGTTTCTTTTACCTTTAGATTAGAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

350

Amino Acids

39.33

Weight (kDa)

8.71

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 107 - 318 2.4e-41 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 265, 923
AciI CCGC 1 cut(s) 232
AclWI GGATC 2 cut(s) 55, 615
AcsI RAATTY 1 cut(s) 338
AcuI CTGAAG 2 cut(s) 54, 581
AfaI GTAC 1 cut(s) 690
AfiI CCNNNNNNNGG 2 cut(s) 265, 923
AflII CTTAAG 1 cut(s) 931
AgsI TTSAA 4 cut(s) 91, 280, 325, 338
AhlI ACTAGT 1 cut(s) 1018
AjnI CCWGG 3 cut(s) 169, 282, 916
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
AluBI AGCT 5 cut(s) 20, 32, 74, 348, 656
AluI AGCT 5 cut(s) 20, 32, 74, 348, 656
Alw26I GTCTC 1 cut(s) 323
AlwI GGATC 2 cut(s) 55, 615
Ama87I CYCGRG 1 cut(s) 343
ApeKI GCWGC 4 cut(s) 29, 365, 491, 841
ApoI RAATTY 1 cut(s) 338
AspLEI GCGC 1 cut(s) 984
AspS9I GGNCC 2 cut(s) 240, 484
AsuHPI GGTGA 1 cut(s) 155
AsuNHI GCTAGC 1 cut(s) 656
AvaI CYCGRG 1 cut(s) 343
AvaII GGWCC 2 cut(s) 240, 484
AxyI CCTNAGG 1 cut(s) 603
BanII GRGCYC 1 cut(s) 758
BbsI GAAGAC 1 cut(s) 775
BbvI GCAGC 4 cut(s) 41, 377, 478, 828
BccI CCATC 5 cut(s) 259, 386, 481, 541, 850
BciT130I CCWGG 3 cut(s) 171, 284, 918
BcoDI GTCTC 1 cut(s) 323
BcuI ACTAGT 1 cut(s) 1018
BfaI CTAG 4 cut(s) 71, 120, 657, 1019
BfmI CTRYAG 1 cut(s) 611
BfrI CTTAAG 1 cut(s) 931
BisI GCNGC 4 cut(s) 30, 366, 492, 842
BlsI GCNGC 4 cut(s) 31, 367, 493, 843
Bme1390I CCNGG 3 cut(s) 171, 284, 918
Bme18I GGWCC 2 cut(s) 240, 484
BmeT110I CYCGRG 1 cut(s) 343
BmgT120I GGNCC 2 cut(s) 240, 484
BmiI GGNNCC 4 cut(s) 109, 485, 486, 861
BmrFI CCNGG 3 cut(s) 171, 284, 918
BmtI GCTAGC 1 cut(s) 660
BpiI GAAGAC 1 cut(s) 775
BpmI CTGGAG 1 cut(s) 1022
BpuEI CTTGAG 1 cut(s) 420
BsaI GGTCTC 1 cut(s) 323
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 923
Bse1I ACTGG 1 cut(s) 744
Bse21I CCTNAGG 1 cut(s) 603
BseBI CCWGG 3 cut(s) 171, 284, 918
BseLI CCNNNNNNNGG 2 cut(s) 265, 923
BseMII CTCAG 2 cut(s) 594, 719
BseNI ACTGG 1 cut(s) 744
BseRI GAGGAG 1 cut(s) 577
BseXI GCAGC 4 cut(s) 41, 377, 478, 828
BseYI CCCAGC 1 cut(s) 299
BsiHKCI CYCGRG 1 cut(s) 343
BslFI GGGAC 2 cut(s) 497, 535
BslI CCNNNNNNNGG 2 cut(s) 265, 923
BsmAI GTCTC 1 cut(s) 323
BsmFI GGGAC 2 cut(s) 497, 535
Bso31I GGTCTC 1 cut(s) 323
BsoBI CYCGRG 1 cut(s) 343
Bsp1286I GDGCHC 1 cut(s) 758
Bsp143I GATC 3 cut(s) 47, 67, 607
BspACI CCGC 1 cut(s) 232
BspCNI CTCAG 2 cut(s) 595, 720
BspLI GGNNCC 4 cut(s) 109, 485, 486, 861
BspMAI CTGCAG 1 cut(s) 615
BspOI GCTAGC 1 cut(s) 660
BspPI GGATC 2 cut(s) 55, 615
BspQI GCTCTTC 1 cut(s) 12
BspTI CTTAAG 1 cut(s) 931
BspTNI GGTCTC 1 cut(s) 323
BsrI ACTGG 1 cut(s) 744
BssMI GATC 3 cut(s) 47, 67, 607
Bst2UI CCWGG 3 cut(s) 171, 284, 918
Bst4CI ACNGT 2 cut(s) 87, 850
Bst6I CTCTTC 2 cut(s) 12, 326
BstAFI CTTAAG 1 cut(s) 931
BstC8I GCNNGC 2 cut(s) 465, 658
BstDEI CTNAG 3 cut(s) 603, 728, 956
BstHHI GCGC 1 cut(s) 984
BstKTI GATC 3 cut(s) 50, 70, 610
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 3 cut(s) 47, 67, 607
BstMWI GCNNNNNNNGC 5 cut(s) 26, 38, 460, 497, 988
BstNI CCWGG 3 cut(s) 171, 284, 918
BstNSI RCATGY 1 cut(s) 458
BstSCI CCNGG 3 cut(s) 169, 282, 916
BstSFI CTRYAG 1 cut(s) 611
BstV1I GCAGC 4 cut(s) 41, 377, 478, 828
BstV2I GAAGAC 1 cut(s) 775
BstX2I RGATCY 2 cut(s) 47, 607
BstXI CCANNNNNNTGG 2 cut(s) 480, 751
BstYI RGATCY 2 cut(s) 47, 607
Bsu36I CCTNAGG 1 cut(s) 603
Cac8I GCNNGC 2 cut(s) 465, 658
CfoI GCGC 1 cut(s) 984
Cfr13I GGNCC 2 cut(s) 240, 484
CseI GACGC 2 cut(s) 371, 532
CsiI ACCWGGT 1 cut(s) 169
Csp6I GTAC 1 cut(s) 689
CviAII CATG 6 cut(s) 331, 455, 480, 787, 877, 938
CviQI GTAC 1 cut(s) 689
DdeI CTNAG 3 cut(s) 603, 728, 956
DpnI GATC 3 cut(s) 49, 69, 609
DpnII GATC 3 cut(s) 47, 67, 607
Eam1104I CTCTTC 2 cut(s) 12, 326
EarI CTCTTC 2 cut(s) 12, 326
Eco24I GRGCYC 1 cut(s) 758
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 2 cut(s) 240, 484
Eco57I CTGAAG 2 cut(s) 54, 581
Eco81I CCTNAGG 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 484
EcoRI GAATTC 1 cut(s) 338
EcoRII CCWGG 3 cut(s) 169, 282, 916
EcoT22I ATGCAT 1 cut(s) 831
EcoT38I GRGCYC 1 cut(s) 758
FaeI CATG 6 cut(s) 334, 458, 483, 790, 880, 941
FaqI GGGAC 2 cut(s) 497, 535
FatI CATG 6 cut(s) 330, 454, 479, 786, 876, 937
Fnu4HI GCNGC 4 cut(s) 30, 366, 492, 842
FriOI GRGCYC 1 cut(s) 758
Fsp4HI GCNGC 4 cut(s) 30, 366, 492, 842
FspBI CTAG 4 cut(s) 71, 120, 657, 1019
GlaI GCGC 1 cut(s) 983
GluI GCNGC 4 cut(s) 30, 366, 492, 842
GsaI CCCAGC 1 cut(s) 303
GsuI CTGGAG 1 cut(s) 1022
HgaI GACGC 2 cut(s) 371, 532
HhaI GCGC 1 cut(s) 984
Hin1II CATG 6 cut(s) 334, 458, 483, 790, 880, 941
Hin6I GCGC 1 cut(s) 982
HinP1I GCGC 1 cut(s) 982
HinfI GANTC 3 cut(s) 116, 177, 927
HphI GGTGA 1 cut(s) 155
Hpy188I TCNGA 3 cut(s) 244, 682, 855
Hpy188III TCNNGA 3 cut(s) 120, 343, 1001
HpyAV CCTTC 1 cut(s) 134
HpyCH4III ACNGT 2 cut(s) 87, 850
HpyCH4IV ACGT 1 cut(s) 896
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 38, 460, 497, 988
HpyF3I CTNAG 3 cut(s) 603, 728, 956
HpySE526I ACGT 1 cut(s) 896
Hsp92II CATG 6 cut(s) 334, 458, 483, 790, 880, 941
HspAI GCGC 1 cut(s) 982
KflI GGGWCCC 1 cut(s) 484
Kzo9I GATC 3 cut(s) 47, 67, 607
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 223, 718, 859
Lsp1109I GCAGC 4 cut(s) 41, 377, 478, 828
MabI ACCWGGT 1 cut(s) 169
MaeI CTAG 4 cut(s) 71, 120, 657, 1019
MaeII ACGT 1 cut(s) 896
MaeIII GTNAC 1 cut(s) 441
MalI GATC 3 cut(s) 49, 69, 609
MboI GATC 3 cut(s) 47, 67, 607
MboII GAAGA 4 cut(s) 242, 313, 775, 1003
MflI RGATCY 2 cut(s) 47, 607
MhlI GDGCHC 1 cut(s) 758
MluCI AATT 4 cut(s) 151, 338, 531, 891
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 9 cut(s) 98, 545, 598, 598, 622, 636, 766, 789, 794
Mph1103I ATGCAT 1 cut(s) 831
MseI TTAA 6 cut(s) 135, 459, 543, 623, 906, 932
MslI CAYNNNNRTG 2 cut(s) 478, 1019
MspA1I CMGCKG 1 cut(s) 32
MspCI CTTAAG 1 cut(s) 931
MspR9I CCNGG 3 cut(s) 171, 284, 918
MvaI CCWGG 3 cut(s) 171, 284, 918
MwoI GCNNNNNNNGC 5 cut(s) 26, 38, 460, 497, 988
NdeII GATC 3 cut(s) 47, 67, 607
NheI GCTAGC 1 cut(s) 656
NlaIII CATG 6 cut(s) 334, 458, 483, 790, 880, 941
NlaIV GGNNCC 4 cut(s) 109, 485, 486, 861
NsiI ATGCAT 1 cut(s) 831
NspI RCATGY 1 cut(s) 458
PaeR7I CTCGAG 1 cut(s) 343
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 3 cut(s) 116, 177, 927
PflFI GACNNNGTC 1 cut(s) 779
PflMI CCANNNNNTGG 2 cut(s) 265, 923
PfoI TCCNGGA 1 cut(s) 916
PkrI GCNGC 4 cut(s) 31, 367, 493, 843
PpuMI RGGWCCY 1 cut(s) 484
Psp5II RGGWCCY 1 cut(s) 484
Psp6I CCWGG 3 cut(s) 169, 282, 916
PspFI CCCAGC 1 cut(s) 299
PspGI CCWGG 3 cut(s) 169, 282, 916
PspN4I GGNNCC 4 cut(s) 109, 485, 486, 861
PspPI GGNCC 2 cut(s) 240, 484
PspPPI RGGWCCY 1 cut(s) 484
PstI CTGCAG 1 cut(s) 615
PsuI RGATCY 2 cut(s) 47, 607
PsyI GACNNNGTC 1 cut(s) 779
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 1 cut(s) 690
RsaNI GTAC 1 cut(s) 689
RseI CAYNNNNRTG 2 cut(s) 478, 1019
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 6 cut(s) 135, 459, 543, 623, 906, 932
SatI GCNGC 4 cut(s) 30, 366, 492, 842
Sau3AI GATC 3 cut(s) 47, 67, 607
Sau96I GGNCC 2 cut(s) 240, 484
ScrFI CCNGG 3 cut(s) 171, 284, 918
SduI GDGCHC 1 cut(s) 758
SexAI ACCWGGT 1 cut(s) 169
SfcI CTRYAG 1 cut(s) 611
Sfr274I CTCGAG 1 cut(s) 343
SinI GGWCC 2 cut(s) 240, 484
SlaI CTCGAG 1 cut(s) 343
SmiMI CAYNNNNRTG 2 cut(s) 478, 1019
SmlI CTYRAG 3 cut(s) 343, 435, 931
SmoI CTYRAG 3 cut(s) 343, 435, 931
SpeI ACTAGT 1 cut(s) 1018
Sse9I AATT 4 cut(s) 151, 338, 531, 891
SsiI CCGC 1 cut(s) 232
SspMI CTAG 4 cut(s) 71, 120, 657, 1019
StyD4I CCNGG 3 cut(s) 169, 282, 916
TaaI ACNGT 2 cut(s) 87, 850
TaiI ACGT 1 cut(s) 899
TaqI TCGA 1 cut(s) 344
TasI AATT 4 cut(s) 151, 338, 531, 891
TatI WGTACW 1 cut(s) 688
TfiI GAWTC 3 cut(s) 116, 177, 927
Tru1I TTAA 6 cut(s) 135, 459, 543, 623, 906, 932
Tru9I TTAA 6 cut(s) 135, 459, 543, 623, 906, 932
TseI GCWGC 4 cut(s) 29, 365, 491, 841
TspDTI ATGAA 4 cut(s) 391, 775, 849, 1003
TspGWI ACGGA 1 cut(s) 437
Tth111I GACNNNGTC 1 cut(s) 779
Van91I CCANNNNNTGG 2 cut(s) 265, 923
Vha464I CTTAAG 1 cut(s) 931
VpaK11BI GGWCC 2 cut(s) 240, 484
XapI RAATTY 1 cut(s) 338
XbaI TCTAGA 1 cut(s) 119
XceI RCATGY 1 cut(s) 458
XhoI CTCGAG 1 cut(s) 343
XspI CTAG 4 cut(s) 71, 120, 657, 1019
Zsp2I ATGCAT 1 cut(s) 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.