MD08G1242200.v1.1

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007444
Physical Location & Seq
Reverse (-)
30780154 .. 30785762
5609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1242200.v1.1.491

Sequence Viewer

Length: 1815 bp
ATGGCGCTTAAGGCTCCGGCTACGAAAGCTGCTGAAATTGCAGTTGCATCGATTGGGCATGGATACGATATATCCATTGATTTACGGCTCAAGTACTGTAAAGGGGAGTCTCACACTGCTCGATTGATTGAAATCGATGACGACGATGGAGGTCGCGAGGTTGTTCTTCCGGGTGGGATTACCATCCCAAATGTTCCCAAATCGATTAAATGCGATAAAGGAGAGCGCACACGATTCAGGTCCGATGTTATCCCTTTCCAACAGATGTCAGAGCAGTTCAACCAGGAAATATCTTTGACTGGAAAAATACCCTCGGGTCTCTTCAATTCTATGTTTGAATTCTCAGGTTGTTGGCAGAAAGATGCAGCCAACACTAAGACCCTTGCTTTTGATGGGGTGTCCATCACTCTCTATTCTGTTGCACTAGAAAAATCTCAGATGGTACTGCACGAGAATGTTAAAAAAGCAGTCCCATCATTGTGGGACCCTTCTGCATTAGCAAGGTTTATAGAGACATTTGGAACGCATATTGTTGTTGGTGTGAAGATGGGAGGGAAAGATGTTATATATGTGAAGCAGCAGCATTCTTCAACTCTTCAACCGGCTGATATACAGAAAAAACTAAAGGATATGGCAGATAAAAGGTTTTTAGACGCCAATGGTCAGTCTGGTATGCCTTCGGAACAAGGTTACAAGAATGGCAAGTTTGAAATCAGGGAGCAGCGGTTGCAGTTTGCAGATACTAGTCCATCAAGTACTTATTCACACAAGGAGGACATTGTAAGCATTTGCAAGAGGAGAGGTGGAAGTGATACGAGAAATCTATCACATAATGAATGGTTACAAACTGTTCAGTTGGAGCCTGATGTGATCACAATGTCATTTATACCAATTACCACTCTGTTGAATGGAGTCCCTGGGAGTGGATTCTTGAGCCATGCCATAAACCTTTATTTACGATATAAACCACCAATTGAAGAACTCCATCAGTTTTTGGAGTTTCAGCTGCCGAGGCAATGGGCACCAGTTTTTAGCGACCTTCCCCTTGGTCCACAGCGGAAGCAACAAAGCAATGTATCTCTACAGTTTAGTTTAATGGGGCCCAAACTTTACGTGAACACCAGTCTGGTTGATGTGGGAAAGAGGCCAGTGACTGGCCTGCGACTGTATCTAGAGGGCAAAAGAAGCAACCGTTTGGCCATTCACTTACAACACCTCTCCTCTCTTCCAAAAATATTCCAACTCGAAGATGATCCAAATGGAAACTTTCGTCGCGATCCCTATGATCGTAAATACTATGAGAAAGTCCAGTGGAAGAACTTCTCCCATGTCTGCACTGCCCCTGTGGAGTCCGATGAGGATCATTCAATAGTAACTGGAGCCCAGTTACAGGTTGAGAATTATGGAATAAAGAATATTCTCTTCTTGAGACTCCGCTTCTCTACTGTTTTCAGCGCAGCAATTGTAAAGCGTCCTGAGTGGGATGGATCACCAGGTTTGGCACATAAGTCCGGACTCATATCAACACTAATCAGCCATCATTTTACAACAGTTCAGAAGCCACCTCCACGGCCAGCAGATGTGAACATAAACTCTGCCGTTTACCCTGGAGGTCCTCCGGTTCCTATCCAAGCCTCAAAGCTTCGGAAATTCGTTGACACAACAGAGATGGTAAGAGGGCCACAGGAGACTCCAGGTTATTGGGTTGTCTCTGGGGCAAGGCTAGTGGTTGAGAAGGGCAGGATATCACTCCGTGTGAAATATTCTTTATTGACTGTAATTTTACCTGACGAAGAAGAGTTGGAGGGACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

605

Amino Acids

67.57

Weight (kDa)

8.86

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 107 - 318 8.2e-42 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1021
AccB7I CCANNNNNTGG 1 cut(s) 1154
AccII CGCG 2 cut(s) 156, 1275
AccIII TCCGGA 1 cut(s) 1511
AciI CCGC 3 cut(s) 724, 1057, 1435
AclWI GGATC 4 cut(s) 1247, 1271, 1368, 1495
AcoI YGGCCR 2 cut(s) 1197, 1571
AcsI RAATTY 2 cut(s) 338, 1649
AcyI GRCGYC 1 cut(s) 654
AdeI CACNNNGTG 1 cut(s) 1754
AfaI GTAC 3 cut(s) 95, 444, 757
AfiI CCNNNNNNNGG 3 cut(s) 923, 1154, 1390
AflII CTTAAG 1 cut(s) 8
AhlI ACTAGT 1 cut(s) 743
AjnI CCWGG 5 cut(s) 282, 916, 1492, 1606, 1693
AluBI AGCT 3 cut(s) 29, 1006, 1642
AluI AGCT 3 cut(s) 29, 1006, 1642
Alw26I GTCTC 6 cut(s) 114, 323, 506, 1423, 1682, 1714
AlwI GGATC 4 cut(s) 1247, 1271, 1368, 1495
AlwNI CAGNNNCTG 1 cut(s) 1154
Ama87I CYCGRG 1 cut(s) 313
Aor13HI TCCGGA 1 cut(s) 1511
AoxI GGCC 6 cut(s) 1100, 1145, 1156, 1197, 1571, 1679
ApaI GGGCCC 1 cut(s) 1104
ApeKI GCWGC 7 cut(s) 29, 365, 577, 580, 721, 1006, 1457
ApoI RAATTY 2 cut(s) 338, 1649
Asp700I GAANNNNTTC 1 cut(s) 1319
AspLEI GCGC 3 cut(s) 7, 228, 1457
AspS9I GGNCC 7 cut(s) 240, 484, 1049, 1100, 1101, 1613, 1679
AsuC2I CCSGG 1 cut(s) 171
AsuHPI GGTGA 1 cut(s) 1482
AvaI CYCGRG 1 cut(s) 313
AvaII GGWCC 4 cut(s) 240, 484, 1049, 1613
BaeGI GKGCMC 2 cut(s) 1024, 1104
BalI TGGCCA 1 cut(s) 1199
BanI GGYRCC 1 cut(s) 1021
BanII GRGCYC 2 cut(s) 1104, 1384
BauI CACGAG 1 cut(s) 449
BbvI GCAGC 7 cut(s) 16, 377, 589, 592, 733, 993, 1469
BceAI ACGGC 3 cut(s) 101, 1583, 1586
BcgI CGANNNNNNTGC 2 cut(s) 30, 64
BciT130I CCWGG 5 cut(s) 284, 918, 1494, 1608, 1695
BciVI GTATCC 1 cut(s) 56
BclI TGATCA 1 cut(s) 870
BcnI CCSGG 1 cut(s) 171
BcoDI GTCTC 6 cut(s) 114, 323, 506, 1423, 1682, 1714
BcuI ACTAGT 1 cut(s) 743
BfaI CTAG 4 cut(s) 425, 744, 1172, 1724
BfmI CTRYAG 1 cut(s) 1082
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 8
BfuI GTATCC 1 cut(s) 56
BisI GCNGC 7 cut(s) 30, 366, 578, 581, 722, 1007, 1458
BlsI GCNGC 7 cut(s) 31, 367, 579, 582, 723, 1008, 1459
BmcAI AGTACT 2 cut(s) 95, 757
Bme1390I CCNGG 6 cut(s) 171, 284, 918, 1494, 1608, 1695
Bme18I GGWCC 4 cut(s) 240, 484, 1049, 1613
BmeT110I CYCGRG 1 cut(s) 313
BmgT120I GGNCC 7 cut(s) 240, 484, 1049, 1100, 1101, 1613, 1679
BmiI GGNNCC 9 cut(s) 15, 485, 486, 861, 1023, 1101, 1102, 1381, 1623
BmrFI CCNGG 6 cut(s) 171, 284, 918, 1494, 1608, 1695
BmrI ACTGGG 1 cut(s) 1378
BmsI GCATC 2 cut(s) 56, 352
BmuI ACTGGG 1 cut(s) 1378
BpmI CTGGAG 3 cut(s) 1398, 1629, 1677
BpuEI CTTGAG 3 cut(s) 74, 952, 1447
BpuMI CCSGG 1 cut(s) 171
Bsa29I ATCGAT 3 cut(s) 50, 135, 203
BsaAI YACGTR 1 cut(s) 1114
BsaBI GATNNNNATC 3 cut(s) 131, 182, 1359
BsaHI GRCGYC 1 cut(s) 654
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 7 cut(s) 312, 916, 917, 1010, 1045, 1568, 1606
BsaWI WCCGGW 2 cut(s) 1511, 1618
BsaXI ACNNNNNCTCC 3 cut(s) 710, 740, 1796
Bsc4I CCNNNNNNNGG 3 cut(s) 923, 1154, 1390
Bse118I RCCGGY 1 cut(s) 601
Bse1I ACTGG 8 cut(s) 304, 1025, 1122, 1148, 1159, 1309, 1381, 1384
Bse3DI GCAATG 2 cut(s) 1022, 1078
Bse8I GATNNNNATC 3 cut(s) 131, 182, 1359
BseAI TCCGGA 1 cut(s) 1511
BseBI CCWGG 5 cut(s) 284, 918, 1494, 1608, 1695
BseCI ATCGAT 3 cut(s) 50, 135, 203
BseDI CCNNGG 7 cut(s) 312, 916, 917, 1010, 1045, 1568, 1606
BseGI GGATG 2 cut(s) 183, 1489
BseJI GATNNNNATC 3 cut(s) 131, 182, 1359
BseLI CCNNNNNNNGG 3 cut(s) 923, 1154, 1390
BseMI GCAATG 2 cut(s) 1022, 1078
BseMII CTCAG 3 cut(s) 357, 449, 1467
BseNI ACTGG 8 cut(s) 304, 1025, 1122, 1148, 1159, 1309, 1381, 1384
BseRI GAGGAG 2 cut(s) 811, 1210
BseSI GKGCMC 2 cut(s) 1024, 1104
BseXI GCAGC 7 cut(s) 16, 377, 589, 592, 733, 993, 1469
BsgI GTGCAG 2 cut(s) 431, 1318
Bsh1236I CGCG 2 cut(s) 156, 1275
BshFI GGCC 6 cut(s) 1102, 1147, 1158, 1199, 1573, 1681
BshNI GGYRCC 1 cut(s) 1021
BshVI ATCGAT 3 cut(s) 50, 135, 203
BsiHKCI CYCGRG 1 cut(s) 313
BsiSI CCGG 5 cut(s) 17, 170, 602, 1512, 1619
BslFI GGGAC 3 cut(s) 455, 497, 899
BslI CCNNNNNNNGG 3 cut(s) 923, 1154, 1390
BsmAI GTCTC 6 cut(s) 114, 323, 506, 1423, 1682, 1714
BsmFI GGGAC 3 cut(s) 455, 497, 899
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 6 cut(s) 1102, 1147, 1158, 1199, 1573, 1681
Bso31I GGTCTC 1 cut(s) 323
BsoBI CYCGRG 1 cut(s) 313
Bsp120I GGGCCC 1 cut(s) 1100
Bsp1286I GDGCHC 3 cut(s) 1024, 1104, 1384
Bsp13I TCCGGA 1 cut(s) 1511
Bsp143I GATC 6 cut(s) 870, 1252, 1276, 1285, 1360, 1487
Bsp68I TCGCGA 2 cut(s) 156, 1275
BspACI CCGC 3 cut(s) 724, 1057, 1435
BspANI GGCC 6 cut(s) 1102, 1147, 1158, 1199, 1573, 1681
BspCNI CTCAG 3 cut(s) 356, 448, 1468
BspDI ATCGAT 3 cut(s) 50, 135, 203
BspEI TCCGGA 1 cut(s) 1511
BspFNI CGCG 2 cut(s) 156, 1275
BspLI GGNNCC 9 cut(s) 15, 485, 486, 861, 1023, 1101, 1102, 1381, 1623
BspPI GGATC 4 cut(s) 1247, 1271, 1368, 1495
BspT107I GGYRCC 1 cut(s) 1021
BspTI CTTAAG 1 cut(s) 8
BspTNI GGTCTC 1 cut(s) 323
BsrDI GCAATG 2 cut(s) 1022, 1078
BsrFI RCCGGY 1 cut(s) 601
BsrI ACTGG 8 cut(s) 304, 1025, 1122, 1148, 1159, 1309, 1381, 1384
BssAI RCCGGY 1 cut(s) 601
BssECI CCNNGG 7 cut(s) 312, 916, 917, 1010, 1045, 1568, 1606
BssMI GATC 6 cut(s) 870, 1252, 1276, 1285, 1360, 1487
BssNI GRCGYC 1 cut(s) 654
BssSI CACGAG 1 cut(s) 449
BssT1I CCWWGG 1 cut(s) 1045
Bst2BI CACGAG 1 cut(s) 449
Bst2UI CCWGG 5 cut(s) 284, 918, 1494, 1608, 1695
Bst4CI ACNGT 9 cut(s) 98, 850, 1086, 1167, 1193, 1447, 1552, 1777, 1812
Bst6I CTCTTC 5 cut(s) 326, 600, 1230, 1427, 1791
BstACI GRCGYC 1 cut(s) 654
BstAFI CTTAAG 1 cut(s) 8
BstAPI GCANNNNNTGC 1 cut(s) 727
BstBAI YACGTR 1 cut(s) 1114
BstC8I GCNNGC 2 cut(s) 1160, 1575
BstDEI CTNAG 4 cut(s) 343, 375, 435, 1476
BstDSI CCRYGG 1 cut(s) 1568
BstF5I GGATG 2 cut(s) 183, 1489
BstFNI CGCG 2 cut(s) 156, 1275
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 228, 1457
BstKTI GATC 6 cut(s) 873, 1255, 1279, 1288, 1363, 1490
BstMAI GTCTC 6 cut(s) 114, 323, 506, 1423, 1682, 1714
BstMBI GATC 6 cut(s) 870, 1252, 1276, 1285, 1360, 1487
BstMWI GCNNNNNNNGC 6 cut(s) 11, 26, 38, 727, 1012, 1185
BstNI CCWGG 5 cut(s) 284, 918, 1494, 1608, 1695
BstSCI CCNGG 6 cut(s) 169, 282, 916, 1492, 1606, 1693
BstSFI CTRYAG 1 cut(s) 1082
BstSLI GKGCMC 2 cut(s) 1024, 1104
BstUI CGCG 2 cut(s) 156, 1275
BstV1I GCAGC 7 cut(s) 16, 377, 589, 592, 733, 993, 1469
BstXI CCANNNNNNTGG 2 cut(s) 480, 1701
Bsu15I ATCGAT 3 cut(s) 50, 135, 203
BsuI GTATCC 1 cut(s) 56
BsuRI GGCC 6 cut(s) 1102, 1147, 1158, 1199, 1573, 1681
BsuTUI ATCGAT 3 cut(s) 50, 135, 203
BtgI CCRYGG 1 cut(s) 1568
BtsCI GGATG 2 cut(s) 183, 1489
BtsI GCAGTG 2 cut(s) 114, 1335
BtsIMutI CAGTG 4 cut(s) 114, 1155, 1316, 1335
BtuMI TCGCGA 2 cut(s) 156, 1275
Cac8I GCNNGC 2 cut(s) 1160, 1575
CaiI CAGNNNCTG 1 cut(s) 1154
CfoI GCGC 3 cut(s) 7, 228, 1457
Cfr10I RCCGGY 1 cut(s) 601
Cfr13I GGNCC 7 cut(s) 240, 484, 1049, 1100, 1101, 1613, 1679
ClaI ATCGAT 3 cut(s) 50, 135, 203
CseI GACGC 2 cut(s) 662, 1460
CsiI ACCWGGT 1 cut(s) 1492
Csp6I GTAC 3 cut(s) 94, 443, 756
CspCI CAANNNNNGTGG 2 cut(s) 1707, 1742
CviAII CATG 3 cut(s) 59, 938, 1328
CviQI GTAC 3 cut(s) 94, 443, 756
DdeI CTNAG 4 cut(s) 343, 375, 435, 1476
DpnI GATC 6 cut(s) 872, 1254, 1278, 1287, 1362, 1489
DpnII GATC 6 cut(s) 870, 1252, 1276, 1285, 1360, 1487
DraIII CACNNNGTG 1 cut(s) 1754
EaeI YGGCCR 2 cut(s) 1197, 1571
Eam1104I CTCTTC 5 cut(s) 326, 600, 1230, 1427, 1791
EarI CTCTTC 5 cut(s) 326, 600, 1230, 1427, 1791
Eco130I CCWWGG 1 cut(s) 1045
Eco24I GRGCYC 2 cut(s) 1104, 1384
Eco31I GGTCTC 1 cut(s) 323
Eco32I GATATC 1 cut(s) 1746
Eco47I GGWCC 4 cut(s) 240, 484, 1049, 1613
Eco88I CYCGRG 1 cut(s) 313
EcoO109I RGGNCCY 3 cut(s) 484, 1100, 1613
EcoRI GAATTC 1 cut(s) 338
EcoRII CCWGG 5 cut(s) 282, 916, 1492, 1606, 1693
EcoRV GATATC 1 cut(s) 1746
EcoT14I CCWWGG 1 cut(s) 1045
EcoT38I GRGCYC 2 cut(s) 1104, 1384
ErhI CCWWGG 1 cut(s) 1045
FaeI CATG 3 cut(s) 62, 941, 1331
FaqI GGGAC 3 cut(s) 455, 497, 899
FatI CATG 3 cut(s) 58, 937, 1327
FbaI TGATCA 1 cut(s) 870
Fnu4HI GCNGC 7 cut(s) 30, 366, 578, 581, 722, 1007, 1458
FokI GGATG 2 cut(s) 170, 1496
FriOI GRGCYC 2 cut(s) 1104, 1384
Fsp4HI GCNGC 7 cut(s) 30, 366, 578, 581, 722, 1007, 1458
FspBI CTAG 4 cut(s) 425, 744, 1172, 1724
GlaI GCGC 3 cut(s) 6, 227, 1456
GluI GCNGC 7 cut(s) 30, 366, 578, 581, 722, 1007, 1458
GsuI CTGGAG 3 cut(s) 1398, 1629, 1677
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 1102, 1147, 1158, 1199, 1573, 1681
HapII CCGG 5 cut(s) 17, 170, 602, 1512, 1619
HgaI GACGC 2 cut(s) 662, 1460
HhaI GCGC 3 cut(s) 7, 228, 1457
Hin1I GRCGYC 1 cut(s) 654
Hin1II CATG 3 cut(s) 62, 941, 1331
Hin6I GCGC 3 cut(s) 5, 226, 1455
HinP1I GCGC 3 cut(s) 5, 226, 1455
HincII GTYRAC 1 cut(s) 1657
HindII GTYRAC 1 cut(s) 1657
HindIII AAGCTT 1 cut(s) 1640
HinfI GANTC 8 cut(s) 107, 234, 912, 927, 1349, 1431, 1515, 1690
HpaII CCGG 5 cut(s) 17, 170, 602, 1512, 1619
HphI GGTGA 1 cut(s) 1482
Hpy166II GTNNAC 5 cut(s) 1052, 1117, 1585, 1603, 1657
Hpy188I TCNGA 7 cut(s) 244, 271, 438, 682, 1354, 1557, 1647
Hpy188III TCNNGA 7 cut(s) 155, 931, 1172, 1274, 1426, 1475, 1512
Hpy8I GTNNAC 5 cut(s) 1052, 1117, 1585, 1603, 1657
Hpy99I CGWCG 2 cut(s) 146, 1275
HpyAV CCTTC 4 cut(s) 498, 687, 1049, 1729
HpyCH4III ACNGT 9 cut(s) 98, 850, 1086, 1167, 1193, 1447, 1552, 1777, 1812
HpyCH4IV ACGT 1 cut(s) 1113
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 26, 38, 727, 1012, 1185
HpyF3I CTNAG 4 cut(s) 343, 375, 435, 1476
HpySE526I ACGT 1 cut(s) 1113
Hsp92I GRCGYC 1 cut(s) 654
Hsp92II CATG 3 cut(s) 62, 941, 1331
HspAI GCGC 3 cut(s) 5, 226, 1455
KflI GGGWCCC 1 cut(s) 484
Kpn2I TCCGGA 1 cut(s) 1511
Ksp22I TGATCA 1 cut(s) 870
Kzo9I GATC 6 cut(s) 870, 1252, 1276, 1285, 1360, 1487
LmnI GCTCC 4 cut(s) 19, 718, 859, 1379
Lsp1109I GCAGC 7 cut(s) 16, 377, 589, 592, 733, 993, 1469
LweI GCATC 2 cut(s) 56, 352
MabI ACCWGGT 1 cut(s) 1492
MaeI CTAG 4 cut(s) 425, 744, 1172, 1724
MaeII ACGT 1 cut(s) 1113
MaeIII GTNAC 5 cut(s) 689, 840, 1150, 1372, 1386
MalI GATC 6 cut(s) 872, 1254, 1278, 1287, 1362, 1489
MboI GATC 6 cut(s) 870, 1252, 1276, 1285, 1360, 1487
MfeI CAATTG 2 cut(s) 972, 1461
MhlI GDGCHC 3 cut(s) 1024, 1104, 1384
MlsI TGGCCA 1 cut(s) 1199
MluCI AATT 9 cut(s) 36, 325, 338, 891, 972, 1399, 1461, 1649, 1779
MluNI TGGCCA 1 cut(s) 1199
MlyI GAGTC 6 cut(s) 116, 921, 1358, 1425, 1509, 1684
MmeI TCCRAC 4 cut(s) 283, 837, 1264, 1782
Mox20I TGGCCA 1 cut(s) 1199
MroI TCCGGA 1 cut(s) 1511
MroXI GAANNNNTTC 1 cut(s) 1319
MscI TGGCCA 1 cut(s) 1199
MseI TTAA 4 cut(s) 9, 207, 459, 1094
MslI CAYNNNNRTG 2 cut(s) 453, 478
Msp20I TGGCCA 1 cut(s) 1199
MspA1I CMGCKG 3 cut(s) 724, 1006, 1057
MspCI CTTAAG 1 cut(s) 8
MspI CCGG 5 cut(s) 17, 170, 602, 1512, 1619
MspR9I CCNGG 6 cut(s) 171, 284, 918, 1494, 1608, 1695
MunI CAATTG 2 cut(s) 972, 1461
Mva1269I GAATGC 1 cut(s) 583
MvaI CCWGG 5 cut(s) 284, 918, 1494, 1608, 1695
MvnI CGCG 2 cut(s) 156, 1275
MwoI GCNNNNNNNGC 6 cut(s) 11, 26, 38, 727, 1012, 1185
NciI CCSGG 1 cut(s) 171
NdeII GATC 6 cut(s) 870, 1252, 1276, 1285, 1360, 1487
NlaIII CATG 3 cut(s) 62, 941, 1331
NlaIV GGNNCC 9 cut(s) 15, 485, 486, 861, 1023, 1101, 1102, 1381, 1623
NmeAIII GCCGAG 1 cut(s) 1035
NmuCI GTSAC 1 cut(s) 1150
NruI TCGCGA 2 cut(s) 156, 1275
PasI CCCWGGG 1 cut(s) 917
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 1 cut(s) 583
PdmI GAANNNNTTC 1 cut(s) 1319
PfeI GAWTC 2 cut(s) 234, 927
PflMI CCANNNNNTGG 1 cut(s) 1154
PkrI GCNGC 7 cut(s) 31, 367, 579, 582, 723, 1008, 1459
PleI GAGTC 6 cut(s) 115, 920, 1357, 1425, 1509, 1684
PpsI GAGTC 6 cut(s) 115, 920, 1357, 1425, 1509, 1684
Ppu21I YACGTR 1 cut(s) 1114
PpuMI RGGWCCY 2 cut(s) 484, 1613
Psp5II RGGWCCY 2 cut(s) 484, 1613
Psp6I CCWGG 5 cut(s) 282, 916, 1492, 1606, 1693
PspGI CCWGG 5 cut(s) 282, 916, 1492, 1606, 1693
PspN4I GGNNCC 9 cut(s) 15, 485, 486, 861, 1023, 1101, 1102, 1381, 1623
PspOMI GGGCCC 1 cut(s) 1100
PspPI GGNCC 7 cut(s) 240, 484, 1049, 1100, 1101, 1613, 1679
PspPPI RGGWCCY 2 cut(s) 484, 1613
PstNI CAGNNNCTG 1 cut(s) 1154
PvuII CAGCTG 1 cut(s) 1006
RruI TCGCGA 2 cut(s) 156, 1275
RsaI GTAC 3 cut(s) 95, 444, 757
RsaNI GTAC 3 cut(s) 94, 443, 756
RseI CAYNNNNRTG 2 cut(s) 453, 478
SaqAI TTAA 4 cut(s) 9, 207, 459, 1094
SatI GCNGC 7 cut(s) 30, 366, 578, 581, 722, 1007, 1458
Sau3AI GATC 6 cut(s) 870, 1252, 1276, 1285, 1360, 1487
Sau96I GGNCC 7 cut(s) 240, 484, 1049, 1100, 1101, 1613, 1679
ScaI AGTACT 2 cut(s) 95, 757
SchI GAGTC 6 cut(s) 116, 921, 1358, 1425, 1509, 1684
ScrFI CCNGG 6 cut(s) 171, 284, 918, 1494, 1608, 1695
SduI GDGCHC 3 cut(s) 1024, 1104, 1384
SexAI ACCWGGT 1 cut(s) 1492
SfaNI GCATC 2 cut(s) 56, 352
SfcI CTRYAG 1 cut(s) 1082
SinI GGWCC 4 cut(s) 240, 484, 1049, 1613
SmiMI CAYNNNNRTG 2 cut(s) 453, 478
SmlI CTYRAG 4 cut(s) 8, 89, 931, 1426
SmoI CTYRAG 4 cut(s) 8, 89, 931, 1426
SpeI ACTAGT 1 cut(s) 743
Sse9I AATT 9 cut(s) 36, 325, 338, 891, 972, 1399, 1461, 1649, 1779
SsiI CCGC 3 cut(s) 724, 1057, 1435
SspI AATATT 3 cut(s) 1236, 1417, 1763
SspMI CTAG 4 cut(s) 425, 744, 1172, 1724
StyD4I CCNGG 6 cut(s) 169, 282, 916, 1492, 1606, 1693
StyI CCWWGG 1 cut(s) 1045
TaaI ACNGT 9 cut(s) 98, 850, 1086, 1167, 1193, 1447, 1552, 1777, 1812
TaiI ACGT 1 cut(s) 1116
TaqI TCGA 5 cut(s) 50, 121, 135, 203, 1245
TasI AATT 9 cut(s) 36, 325, 338, 891, 972, 1399, 1461, 1649, 1779
TatI WGTACW 2 cut(s) 93, 755
TfiI GAWTC 2 cut(s) 234, 927
Tru1I TTAA 4 cut(s) 9, 207, 459, 1094
Tru9I TTAA 4 cut(s) 9, 207, 459, 1094
TscAI CASTG 4 cut(s) 121, 1155, 1316, 1342
TseFI GTSAC 1 cut(s) 1150
TseI GCWGC 7 cut(s) 29, 365, 577, 580, 721, 1006, 1457
Tsp45I GTSAC 1 cut(s) 1150
TspDTI ATGAA 1 cut(s) 849
TspGWI ACGGA 1 cut(s) 1742
TspRI CASTG 4 cut(s) 121, 1155, 1316, 1342
Van91I CCANNNNNTGG 1 cut(s) 1154
Vha464I CTTAAG 1 cut(s) 8
VpaK11BI GGWCC 4 cut(s) 240, 484, 1049, 1613
XapI RAATTY 2 cut(s) 338, 1649
XbaI TCTAGA 1 cut(s) 1171
XmnI GAANNNNTTC 1 cut(s) 1319
XspI CTAG 4 cut(s) 425, 744, 1172, 1724
ZrmI AGTACT 2 cut(s) 95, 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.