Rorug07G0195600

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0007444
Physical Location & Seq
Reverse (-)
16831918 .. 16835981
4064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0195600.1

Sequence Viewer

Length: 1020 bp
ATGGTGAGGTTTGAGACTGAGGTGGTGTTCGTGGGTTTGGTGGAGGGTGGCAAGTGGATGGTCAAGTCCAAGAGCAAGAGAGGTGAGGATTTGGAAGAGATTTATGATGCTGTGGTTGTCTGTAATGGCCATTCTACTGAGCCCCGTGTGGCCCAAATTGAAGGCATCAGCTCATGGAAAGGAAAGCAAATTCACAGCCACAATTATCGGACTTCTGAGCCATTTCGAGATCAGGTTGTAGTTTTGATAGGGAGCTCTGCTAGTGCTATTGATATTTCTCGGGACATAGCTGGAGTTGTCAAAGAAGTCCACATTGCATCTAGATCCTCTATTGCCTCTGAAACCTCTGAATATAAGCCTGGCTTTCATAACATGTGGCTTAAAGCTATGATAAATAGTGGCTACGATAACATGCGGCTTCACTCTATGATTAAAAGTGTCCATGAAGATGGTAGTGTTGTTTTCGCAGATGGAAGTGTTGTGGTCGCCGACACCATTCTTCATTGCACAGGATACAAATATCATTTCCCTTTTCTTGAGACCAATGGAATTGTAACTGTGGATGGGGATGACAACCGTGTTGGGCCGCTGTTCAAGCATGTCTTCCCACCTGCCTTTGCACCTTCGCTTTCTTTTGTTGGGGTACCATGGAAGACCGTTCCTTTCCCCAATTCTGAACTTCAAAGCAAGTGGATAGCAGGAGTTTTGTCGAATCGAATTGCGCTCCCATCCAAAGAGAAGATGATGGAGGATGTCAAAGCTTTCTACTCTTCACTTGAAGCTTCTGGCAAACCTAAACGACACACTCATGACATTTCTGATTCTCGGGGTGATTATGCTGATTTGCTTGCAGCGCAGTGTGGGTGTCCAGTCTTCGAAGAATGGAGAAAACAAATGATGTTAACAGTAGCAAAGAATTATTTAGTCCGGCCGAAAACATATCAGGATGAGTGGGATGATAACCATTTAGTACTACAAGCTTATAAGGACTTCAAGAAATTCACCACAGATGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

339

Amino Acids

38.0

Weight (kDa)

6.36

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 13 - 184 7.1e-11 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 14 - 109 7.4e-15 Flavin-binding monooxygenase-like
Pyr_redox_3 PF13738 37 - 186 4.6e-12 Pyridine nucleotide-disulphide oxidoreductase
FMO-like PF00743 138 - 256 1.6e-15 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 984
AarI CACCTGC 1 cut(s) 619
Acc36I ACCTGC 1 cut(s) 619
Acc65I GGTACC 1 cut(s) 643
AccB1I GGYRCC 1 cut(s) 643
AciI CCGC 2 cut(s) 415, 587
AclWI GGATC 1 cut(s) 318
AcoI YGGCCR 2 cut(s) 127, 929
AcsI RAATTY 2 cut(s) 189, 998
AfaI GTAC 2 cut(s) 645, 972
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 5 cut(s) 161, 595, 683, 779, 994
AjnI CCWGG 1 cut(s) 358
AluBI AGCT 7 cut(s) 171, 255, 290, 386, 761, 782, 980
AluI AGCT 7 cut(s) 171, 255, 290, 386, 761, 782, 980
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 2 cut(s) 8, 533
AlwI GGATC 1 cut(s) 318
Ama87I CYCGRG 2 cut(s) 279, 825
AoxI GGCC 4 cut(s) 127, 150, 584, 929
ApeKI GCWGC 1 cut(s) 851
ApoI RAATTY 2 cut(s) 189, 998
Asp718I GGTACC 1 cut(s) 643
AspLEI GCGC 2 cut(s) 724, 856
AspS9I GGNCC 2 cut(s) 151, 584
AsuHPI GGTGA 4 cut(s) 16, 95, 842, 994
AsuII TTCGAA 1 cut(s) 876
AvaI CYCGRG 2 cut(s) 279, 825
BalI TGGCCA 1 cut(s) 129
BanI GGYRCC 1 cut(s) 643
BanII GRGCYC 2 cut(s) 144, 257
BbsI GAAGAC 3 cut(s) 595, 659, 865
Bbv12I GWGCWC 1 cut(s) 257
BbvI GCAGC 1 cut(s) 863
BccI CCATC 7 cut(s) 52, 443, 464, 557, 736, 739, 1004
BciT130I CCWGG 1 cut(s) 360
BciVI GTATCC 1 cut(s) 506
BcoDI GTCTC 2 cut(s) 8, 533
BfaI CTAG 2 cut(s) 261, 321
BfuAI ACCTGC 1 cut(s) 619
BfuI GTATCC 1 cut(s) 506
BisI GCNGC 3 cut(s) 416, 587, 852
BlsI GCNGC 3 cut(s) 417, 588, 853
BmcAI AGTACT 1 cut(s) 972
Bme1390I CCNGG 1 cut(s) 360
BmeT110I CYCGRG 2 cut(s) 279, 825
BmgT120I GGNCC 2 cut(s) 151, 584
BmiI GGNNCC 1 cut(s) 645
BmrFI CCNGG 1 cut(s) 360
BmsI GCATC 3 cut(s) 97, 174, 326
BpiI GAAGAC 3 cut(s) 595, 659, 865
BpmI CTGGAG 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 876
BpuEI CTTGAG 1 cut(s) 557
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 1 cut(s) 647
Bse1I ACTGG 1 cut(s) 869
Bse3DI GCAATG 2 cut(s) 312, 502
BseBI CCWGG 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 647
BseGI GGATG 7 cut(s) 63, 568, 574, 728, 757, 952, 961
BseMI GCAATG 2 cut(s) 312, 502
BseMII CTCAG 3 cut(s) 9, 129, 207
BseNI ACTGG 1 cut(s) 869
BseX3I CGGCCG 1 cut(s) 929
BseXI GCAGC 1 cut(s) 863
Bsh1285I CGRYCG 1 cut(s) 932
BshFI GGCC 4 cut(s) 129, 152, 586, 931
BshNI GGYRCC 1 cut(s) 643
BsiEI CGRYCG 1 cut(s) 932
BsiHKAI GWGCWC 1 cut(s) 257
BsiHKCI CYCGRG 2 cut(s) 279, 825
BsiSI CCGG 1 cut(s) 928
BslFI GGGAC 1 cut(s) 296
BsmAI GTCTC 2 cut(s) 8, 533
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 4 cut(s) 129, 152, 586, 931
Bso31I GGTCTC 1 cut(s) 533
BsoBI CYCGRG 2 cut(s) 279, 825
Bsp119I TTCGAA 1 cut(s) 876
Bsp1286I GDGCHC 2 cut(s) 144, 257
Bsp143I GATC 2 cut(s) 229, 323
Bsp19I CCATGG 1 cut(s) 647
BspACI CCGC 2 cut(s) 415, 587
BspANI GGCC 4 cut(s) 129, 152, 586, 931
BspCNI CTCAG 3 cut(s) 10, 130, 208
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 1 cut(s) 645
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 1 cut(s) 318
BspT104I TTCGAA 1 cut(s) 876
BspT107I GGYRCC 1 cut(s) 643
BspTNI GGTCTC 1 cut(s) 533
BsrDI GCAATG 2 cut(s) 312, 502
BsrI ACTGG 1 cut(s) 869
BssECI CCNNGG 1 cut(s) 647
BssMI GATC 2 cut(s) 229, 323
BssT1I CCWWGG 1 cut(s) 647
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 4 cut(s) 559, 578, 658, 907
Bst6I CTCTTC 2 cut(s) 90, 775
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 1 cut(s) 849
BstDEI CTNAG 3 cut(s) 18, 138, 216
BstDSI CCRYGG 1 cut(s) 647
BstF5I GGATG 7 cut(s) 63, 568, 574, 728, 757, 952, 961
BstHHI GCGC 2 cut(s) 724, 856
BstKTI GATC 2 cut(s) 232, 326
BstMAI GTCTC 2 cut(s) 8, 533
BstMBI GATC 2 cut(s) 229, 323
BstMCI CGRYCG 1 cut(s) 932
BstMWI GCNNNNNNNGC 2 cut(s) 595, 853
BstNI CCWGG 1 cut(s) 360
BstNSI RCATGY 3 cut(s) 376, 415, 602
BstSCI CCNGG 1 cut(s) 358
BstV1I GCAGC 1 cut(s) 863
BstV2I GAAGAC 3 cut(s) 595, 659, 865
BstX2I RGATCY 1 cut(s) 323
BstXI CCANNNNNNTGG 1 cut(s) 449
BstYI RGATCY 1 cut(s) 323
BstZI CGGCCG 1 cut(s) 929
BsuI GTATCC 1 cut(s) 506
BsuRI GGCC 4 cut(s) 129, 152, 586, 931
BtgI CCRYGG 1 cut(s) 647
BtsCI GGATG 7 cut(s) 63, 568, 574, 728, 757, 952, 961
BtsI GCAGTG 1 cut(s) 863
BtsIMutI CAGTG 1 cut(s) 863
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 1 cut(s) 849
CciI TCATGA 1 cut(s) 808
CfoI GCGC 2 cut(s) 724, 856
Cfr13I GGNCC 2 cut(s) 151, 584
Csp6I GTAC 2 cut(s) 644, 971
CspCI CAANNNNNGTGG 2 cut(s) 671, 706
CviAII CATG 7 cut(s) 174, 373, 412, 443, 599, 648, 809
CviQI GTAC 2 cut(s) 644, 971
DdeI CTNAG 3 cut(s) 18, 138, 216
DpnI GATC 2 cut(s) 231, 325
DpnII GATC 2 cut(s) 229, 323
EaeI YGGCCR 2 cut(s) 127, 929
EagI CGGCCG 1 cut(s) 929
Eam1104I CTCTTC 2 cut(s) 90, 775
EarI CTCTTC 2 cut(s) 90, 775
Ecl136II GAGCTC 1 cut(s) 255
EclXI CGGCCG 1 cut(s) 929
Eco130I CCWWGG 1 cut(s) 647
Eco24I GRGCYC 2 cut(s) 144, 257
Eco31I GGTCTC 1 cut(s) 533
Eco52I CGGCCG 1 cut(s) 929
Eco53kI GAGCTC 1 cut(s) 255
Eco88I CYCGRG 2 cut(s) 279, 825
EcoICRI GAGCTC 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 647
EcoT38I GRGCYC 2 cut(s) 144, 257
ErhI CCWWGG 1 cut(s) 647
FaeI CATG 7 cut(s) 177, 376, 415, 446, 602, 651, 812
FalI AAGNNNNNCTT 4 cut(s) 347, 379, 587, 619
FaqI GGGAC 1 cut(s) 296
FatI CATG 7 cut(s) 173, 372, 411, 442, 598, 647, 808
Fnu4HI GCNGC 3 cut(s) 416, 587, 852
FokI GGATG 7 cut(s) 70, 575, 581, 715, 764, 959, 968
FriOI GRGCYC 2 cut(s) 144, 257
Fsp4HI GCNGC 3 cut(s) 416, 587, 852
FspBI CTAG 2 cut(s) 261, 321
GlaI GCGC 2 cut(s) 723, 855
GluI GCNGC 3 cut(s) 416, 587, 852
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 4 cut(s) 129, 152, 586, 931
HapII CCGG 1 cut(s) 928
HhaI GCGC 2 cut(s) 724, 856
Hin1II CATG 7 cut(s) 177, 376, 415, 446, 602, 651, 812
Hin6I GCGC 2 cut(s) 722, 854
HinP1I GCGC 2 cut(s) 722, 854
HincII GTYRAC 1 cut(s) 903
HindII GTYRAC 1 cut(s) 903
HindIII AAGCTT 3 cut(s) 759, 780, 978
HinfI GANTC 2 cut(s) 712, 821
HpaI GTTAAC 1 cut(s) 903
HpaII CCGG 1 cut(s) 928
HphI GGTGA 4 cut(s) 16, 95, 842, 994
Hpy166II GTNNAC 2 cut(s) 310, 903
Hpy188I TCNGA 6 cut(s) 210, 217, 340, 349, 676, 820
Hpy188III TCNNGA 7 cut(s) 227, 281, 321, 536, 809, 944, 994
Hpy8I GTNNAC 2 cut(s) 310, 903
HpyAV CCTTC 2 cut(s) 155, 633
HpyCH4III ACNGT 4 cut(s) 559, 578, 658, 907
HpyCH4V TGCA 4 cut(s) 317, 507, 620, 851
HpyF10VI GCNNNNNNNGC 2 cut(s) 595, 853
HpyF3I CTNAG 3 cut(s) 18, 138, 216
Hsp92II CATG 7 cut(s) 177, 376, 415, 446, 602, 651, 812
HspAI GCGC 2 cut(s) 722, 854
KpnI GGTACC 1 cut(s) 647
KspAI GTTAAC 1 cut(s) 903
Kzo9I GATC 2 cut(s) 229, 323
LmnI GCTCC 2 cut(s) 252, 729
Lsp1109I GCAGC 1 cut(s) 863
LweI GCATC 3 cut(s) 97, 174, 326
MaeI CTAG 2 cut(s) 261, 321
MaeIII GTNAC 1 cut(s) 553
MalI GATC 2 cut(s) 231, 325
MboI GATC 2 cut(s) 229, 323
MboII GAAGA 9 cut(s) 107, 458, 491, 595, 664, 751, 762, 865, 890
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 2 cut(s) 144, 257
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 8 cut(s) 156, 189, 202, 549, 670, 717, 916, 998
MluNI TGGCCA 1 cut(s) 129
MnlI CCTC 8 cut(s) 13, 37, 74, 79, 337, 346, 355, 742
Mox20I TGGCCA 1 cut(s) 129
MscI TGGCCA 1 cut(s) 129
MseI TTAA 4 cut(s) 381, 432, 902, 1018
MslI CAYNNNNRTG 2 cut(s) 447, 807
Msp20I TGGCCA 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 589
MspI CCGG 1 cut(s) 928
MspR9I CCNGG 1 cut(s) 360
MvaI CCWGG 1 cut(s) 360
MwoI GCNNNNNNNGC 2 cut(s) 595, 853
NcoI CCATGG 1 cut(s) 647
NdeII GATC 2 cut(s) 229, 323
NlaIII CATG 7 cut(s) 177, 376, 415, 446, 602, 651, 812
NlaIV GGNNCC 1 cut(s) 645
NspI RCATGY 3 cut(s) 376, 415, 602
NspV TTCGAA 1 cut(s) 876
PagI TCATGA 1 cut(s) 808
PaqCI CACCTGC 1 cut(s) 619
PciI ACATGT 1 cut(s) 372
PfeI GAWTC 2 cut(s) 712, 821
PkrI GCNGC 3 cut(s) 417, 588, 853
PscI ACATGT 1 cut(s) 372
PsiI TTATAA 1 cut(s) 984
Psp124BI GAGCTC 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 358
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 1 cut(s) 645
PspPI GGNCC 2 cut(s) 151, 584
PsuI RGATCY 1 cut(s) 323
RsaI GTAC 2 cut(s) 645, 972
RsaNI GTAC 2 cut(s) 644, 971
RseI CAYNNNNRTG 2 cut(s) 447, 807
SacI GAGCTC 1 cut(s) 257
SaqAI TTAA 4 cut(s) 381, 432, 902, 1018
SatI GCNGC 3 cut(s) 416, 587, 852
Sau3AI GATC 2 cut(s) 229, 323
Sau96I GGNCC 2 cut(s) 151, 584
ScaI AGTACT 1 cut(s) 972
ScrFI CCNGG 1 cut(s) 360
SduI GDGCHC 2 cut(s) 144, 257
SfaNI GCATC 3 cut(s) 97, 174, 326
SfuI TTCGAA 1 cut(s) 876
SmiMI CAYNNNNRTG 2 cut(s) 447, 807
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 8 cut(s) 156, 189, 202, 549, 670, 717, 916, 998
SsiI CCGC 2 cut(s) 415, 587
SspMI CTAG 2 cut(s) 261, 321
SstI GAGCTC 1 cut(s) 257
StyD4I CCNGG 1 cut(s) 358
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 4 cut(s) 559, 578, 658, 907
TaqI TCGA 4 cut(s) 226, 710, 715, 876
TasI AATT 8 cut(s) 156, 189, 202, 549, 670, 717, 916, 998
TatI WGTACW 1 cut(s) 970
TauI GCSGC 2 cut(s) 418, 589
TfiI GAWTC 2 cut(s) 712, 821
Tru1I TTAA 4 cut(s) 381, 432, 902, 1018
Tru9I TTAA 4 cut(s) 381, 432, 902, 1018
TscAI CASTG 1 cut(s) 863
TseI GCWGC 1 cut(s) 851
TspDTI ATGAA 3 cut(s) 356, 459, 491
TspRI CASTG 1 cut(s) 863
XapI RAATTY 2 cut(s) 189, 998
XbaI TCTAGA 1 cut(s) 320
XceI RCATGY 3 cut(s) 376, 415, 602
XspI CTAG 2 cut(s) 261, 321
ZrmI AGTACT 1 cut(s) 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.