MD15G1435900.v1.1

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007444
Physical Location & Seq
Reverse (-)
53616159 .. 53622744
6586 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1435900.v1.1.491

Sequence Viewer

Length: 1812 bp
ATGGCGCTTAAGGCTCCAGCTCCGGAAGCTGCCGAGATTGCAATTGCATCCATTGGGCGTGGATACGATATATCCACTGATTTACGGCTCAAGTACTGTAAAGGGGAGTCTCACACTGCTCGATTGATTGAAATCGATGACGATGGAGGCCGCGAGATTGTTCTTCCCGGTGGGATTACCATCCCAAATGTTCCCAAATCGATTAAATGCGATAAAGGAGAGCGCACACGATTCAGGTCCGACGTTCTCTCTTTCCAACAGATGTCAGAGCAGTTCAATCAGGAAATATCGTTGACTGGAAAAATACCCTCAGGTCTCTTCAATTCTATGTTTGATTTCTCAGGTAGTTGGCAGAAAGATGCAGCCAACACTAAGACCCTTGCTTTTGATGGGGTGTTCATCACTCTCTATACTGTTGCACTGGAAAAATCTCAGATGGTACTGTGCGAGAATGTTATTAAAGCAGTCCCATCATCGTGGGAACCTGCTGCATTAGCAAGGTTTATAGAGAGATTTGGAACACATATTGTTGTTGGTGTGAAGATGGGAGGGAAAGATGTTATATATATGAAACAACAGCATTCTTCAACTCTTCAACCGGCTGATATACAGAAAAAACTAAAGGATATGGCAGATAAAAGGTTTTTAGATGCCAATGGTCAGTATGGTATGCCTTCGGAACAAGGTTACAAGAATGGCAAGTTTGAAATCAGGGAGCAGCGGTTAAGGTTTGCAGATACTAGTCCATCAAGTTCCTATTCACAGAAGGAAGACATTTTAAGCATTTGCAAGAGGAGAGGTGGAAGTGAGATGAGAAATATATCCCATAATGAATGGTTACAAACTGTTCAGTTGGAGCCTGATGTGATCACAATGTCATTTATACCAATTACTTCTCTGTTGAATGGAGTCCCTGGGAGTGGATTCTTGAGCCATGCCATAAACCTTTATTTACGATATAAACCACCACTTGAAGAACTCCATCAGTTTTTGGAGTTTCAGCTGCCAAGGCAATGGGCACCGGTTTTTAGTGACCTGCCCCTTGGTCCACAGCGGAAGCAACAAAGCAATGTATCTCTACAGTTTAGCTTAATGGGGCCCAAACTATACGTCAACACTAGTCTGGTTGATGTGGGAAAGAGGCCAGTGACTGGTCTGCGACTGTATTTAGAAGGCAAAAGAAGCAACCGTTTGGCCATTCACTTACAGCACCTCACCTCTCTTCCAAATATCTTCCAACTCGAAGATGATCCAAATGGAAACTTTCGTCGTGATTCCTATGATCGTAAATACTATGAGAAAGTCCAGTGGAAGAACTTCTCCCATGTCTGCACTGCCCCTGTGGAGTCTGATGAGGATCTTTCAATAGTAACTGGAGCCCAGTTACAGGTTGAGAATTATGGAATAAAGAATATCCTCTTCTTGAGACTCCGCTTCTCTACTGTTTCCGGTGCGAGAAATGTAAAGCATCCTGAGTGGGATGGATCACCAGGCTTGGCACATAAGTCCGGACTCATATCAACACTAATCAGCCATCATTTTACAACAGCTCAGAAACCACCTCCACGGCCTGCAGATGTGAACATAAACTCCGCCGTTTACCCTGGAGGTCCTCCGGTACCCATCCAAGCCCCGAAGCTTCGGAAATTCGTTGACACAACAGAGATGGTAAGAGGGCCACAGGAGACTCCGGGTTATTGGGTTGTCTCTGGGGCAAGGCTAGTGGTTGAGAAGGGCAGGATATCCCTCCGTGTGAAATACTCTTTGTTGACTGTAATATTACCTGACGAAGAAGAGTTGGAGGGACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

604

Amino Acids

67.58

Weight (kDa)

8.87

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 106 - 317 4.9e-40 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 493, 1044
Acc65I GGTACC 1 cut(s) 1618
AccB1I GGYRCC 2 cut(s) 1018, 1618
AccB7I CCANNNNNTGG 1 cut(s) 1151
AccII CGCG 1 cut(s) 153
AccIII TCCGGA 2 cut(s) 22, 1508
AciI CCGC 5 cut(s) 151, 721, 1054, 1432, 1593
AclWI GGATC 3 cut(s) 1244, 1365, 1492
AcoI YGGCCR 1 cut(s) 1194
AcsI RAATTY 1 cut(s) 1646
AfaI GTAC 3 cut(s) 95, 441, 1620
AfiI CCNNNNNNNGG 3 cut(s) 920, 1151, 1387
AflII CTTAAG 1 cut(s) 8
AgeI ACCGGT 1 cut(s) 1021
AgsI TTSAA 9 cut(s) 131, 277, 322, 588, 596, 707, 904, 974, 1365
AhlI ACTAGT 2 cut(s) 740, 1118
AjnI CCWGG 3 cut(s) 913, 1489, 1603
AloI GAACNNNNNNTCC 2 cut(s) 1574, 1606
AluBI AGCT 6 cut(s) 20, 29, 1003, 1089, 1550, 1639
AluI AGCT 6 cut(s) 20, 29, 1003, 1089, 1550, 1639
Alw26I GTCTC 5 cut(s) 114, 320, 1420, 1679, 1711
AlwI GGATC 3 cut(s) 1244, 1365, 1492
AlwNI CAGNNNCTG 1 cut(s) 1151
Aor13HI TCCGGA 2 cut(s) 22, 1508
AoxI GGCC 6 cut(s) 148, 1097, 1142, 1194, 1568, 1676
ApaI GGGCCC 1 cut(s) 1101
ApeKI GCWGC 5 cut(s) 29, 362, 488, 718, 1003
ApoI RAATTY 1 cut(s) 1646
ArsI GACNNNNNNTTYG 2 cut(s) 1095, 1127
AsiGI ACCGGT 1 cut(s) 1021
Asp700I GAANNNNTTC 1 cut(s) 1316
Asp718I GGTACC 1 cut(s) 1618
AspLEI GCGC 2 cut(s) 7, 225
AspS9I GGNCC 6 cut(s) 237, 1046, 1097, 1098, 1610, 1676
AsuC2I CCSGG 2 cut(s) 168, 1692
AsuHPI GGTGA 2 cut(s) 1207, 1479
AvaII GGWCC 3 cut(s) 237, 1046, 1610
AxyI CCTNAGG 1 cut(s) 310
BaeGI GKGCMC 2 cut(s) 1021, 1101
BalI TGGCCA 1 cut(s) 1196
BanI GGYRCC 2 cut(s) 1018, 1618
BanII GRGCYC 2 cut(s) 1101, 1381
BbsI GAAGAC 1 cut(s) 777
BbvI GCAGC 5 cut(s) 16, 374, 475, 730, 990
BceAI ACGGC 3 cut(s) 101, 1580, 1583
BciT130I CCWGG 3 cut(s) 915, 1491, 1605
BciVI GTATCC 1 cut(s) 56
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 2 cut(s) 168, 1692
BcoDI GTCTC 5 cut(s) 114, 320, 1420, 1679, 1711
BcuI ACTAGT 2 cut(s) 740, 1118
BfaI CTAG 3 cut(s) 741, 1119, 1721
BfmI CTRYAG 2 cut(s) 1079, 1572
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 8
BfuAI ACCTGC 2 cut(s) 493, 1044
BfuI GTATCC 1 cut(s) 56
BisI GCNGC 6 cut(s) 30, 151, 363, 489, 719, 1004
BlsI GCNGC 6 cut(s) 31, 152, 364, 490, 720, 1005
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 5 cut(s) 168, 915, 1491, 1605, 1692
Bme18I GGWCC 3 cut(s) 237, 1046, 1610
BmgT120I GGNCC 6 cut(s) 237, 1046, 1097, 1098, 1610, 1676
BmiI GGNNCC 8 cut(s) 15, 483, 858, 1020, 1098, 1099, 1378, 1620
BmrFI CCNGG 5 cut(s) 168, 915, 1491, 1605, 1692
BmrI ACTGGG 1 cut(s) 1375
BmsI GCATC 4 cut(s) 56, 349, 640, 1477
BmuI ACTGGG 1 cut(s) 1375
BpiI GAAGAC 1 cut(s) 777
BpmI CTGGAG 2 cut(s) 1395, 1626
BpuEI CTTGAG 3 cut(s) 74, 949, 1444
BpuMI CCSGG 2 cut(s) 168, 1692
Bsa29I ATCGAT 2 cut(s) 135, 200
BsaBI GATNNNNATC 3 cut(s) 131, 179, 1356
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 6 cut(s) 913, 914, 1007, 1042, 1565, 1603
BsaWI WCCGGW 5 cut(s) 22, 1021, 1448, 1508, 1615
BsaXI ACNNNNNCTCC 5 cut(s) 707, 737, 1574, 1604, 1793
Bsc4I CCNNNNNNNGG 3 cut(s) 920, 1151, 1387
Bse118I RCCGGY 2 cut(s) 598, 1021
Bse1I ACTGG 7 cut(s) 301, 426, 1145, 1156, 1306, 1378, 1381
Bse21I CCTNAGG 1 cut(s) 310
Bse3DI GCAATG 2 cut(s) 1019, 1075
Bse8I GATNNNNATC 3 cut(s) 131, 179, 1356
BseAI TCCGGA 2 cut(s) 22, 1508
BseBI CCWGG 3 cut(s) 915, 1491, 1605
BseCI ATCGAT 2 cut(s) 135, 200
BseDI CCNNGG 6 cut(s) 913, 914, 1007, 1042, 1565, 1603
BseGI GGATG 5 cut(s) 47, 180, 1468, 1486, 1623
BseJI GATNNNNATC 3 cut(s) 131, 179, 1356
BseLI CCNNNNNNNGG 3 cut(s) 920, 1151, 1387
BseMI GCAATG 2 cut(s) 1019, 1075
BseMII CTCAG 5 cut(s) 324, 354, 446, 1464, 1565
BseNI ACTGG 7 cut(s) 301, 426, 1145, 1156, 1306, 1378, 1381
BseRI GAGGAG 1 cut(s) 808
BseSI GKGCMC 2 cut(s) 1021, 1101
BseXI GCAGC 5 cut(s) 16, 374, 475, 730, 990
BsgI GTGCAG 1 cut(s) 1315
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 6 cut(s) 150, 1099, 1144, 1196, 1570, 1678
BshNI GGYRCC 2 cut(s) 1018, 1618
BshTI ACCGGT 1 cut(s) 1021
BshVI ATCGAT 2 cut(s) 135, 200
BsiSI CCGG 8 cut(s) 23, 168, 599, 1022, 1449, 1509, 1616, 1691
BslFI GGGAC 2 cut(s) 452, 896
BslI CCNNNNNNNGG 3 cut(s) 920, 1151, 1387
BsmAI GTCTC 5 cut(s) 114, 320, 1420, 1679, 1711
BsmFI GGGAC 2 cut(s) 452, 896
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 6 cut(s) 150, 1099, 1144, 1196, 1570, 1678
Bso31I GGTCTC 1 cut(s) 320
Bsp120I GGGCCC 1 cut(s) 1097
Bsp1286I GDGCHC 3 cut(s) 1021, 1101, 1381
Bsp13I TCCGGA 2 cut(s) 22, 1508
Bsp143I GATC 5 cut(s) 867, 1249, 1282, 1357, 1484
BspACI CCGC 5 cut(s) 151, 721, 1054, 1432, 1593
BspANI GGCC 6 cut(s) 150, 1099, 1144, 1196, 1570, 1678
BspCNI CTCAG 5 cut(s) 323, 353, 445, 1465, 1564
BspDI ATCGAT 2 cut(s) 135, 200
BspEI TCCGGA 2 cut(s) 22, 1508
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 8 cut(s) 15, 483, 858, 1020, 1098, 1099, 1378, 1620
BspMAI CTGCAG 1 cut(s) 1576
BspMI ACCTGC 2 cut(s) 493, 1044
BspPI GGATC 3 cut(s) 1244, 1365, 1492
BspT107I GGYRCC 2 cut(s) 1018, 1618
BspTI CTTAAG 1 cut(s) 8
BspTNI GGTCTC 1 cut(s) 320
BsrDI GCAATG 2 cut(s) 1019, 1075
BsrFI RCCGGY 2 cut(s) 598, 1021
BsrI ACTGG 7 cut(s) 301, 426, 1145, 1156, 1306, 1378, 1381
BssAI RCCGGY 2 cut(s) 598, 1021
BssECI CCNNGG 6 cut(s) 913, 914, 1007, 1042, 1565, 1603
BssMI GATC 5 cut(s) 867, 1249, 1282, 1357, 1484
BssT1I CCWWGG 2 cut(s) 1007, 1042
Bst2UI CCWGG 3 cut(s) 915, 1491, 1605
Bst6I CTCTTC 5 cut(s) 323, 597, 1227, 1424, 1788
BstAFI CTTAAG 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 1572
BstDEI CTNAG 6 cut(s) 310, 340, 372, 432, 1473, 1551
BstDSI CCRYGG 1 cut(s) 1565
BstF5I GGATG 5 cut(s) 47, 180, 1468, 1486, 1623
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 225
BstKTI GATC 5 cut(s) 870, 1252, 1285, 1360, 1487
BstMAI GTCTC 5 cut(s) 114, 320, 1420, 1679, 1711
BstMBI GATC 5 cut(s) 867, 1249, 1282, 1357, 1484
BstMWI GCNNNNNNNGC 6 cut(s) 11, 26, 38, 494, 1009, 1182
BstNI CCWGG 3 cut(s) 915, 1491, 1605
BstSCI CCNGG 5 cut(s) 166, 913, 1489, 1603, 1690
BstSFI CTRYAG 2 cut(s) 1079, 1572
BstSLI GKGCMC 2 cut(s) 1021, 1101
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 5 cut(s) 16, 374, 475, 730, 990
BstV2I GAAGAC 1 cut(s) 777
BstX2I RGATCY 1 cut(s) 1357
BstXI CCANNNNNNTGG 2 cut(s) 477, 1014
BstYI RGATCY 1 cut(s) 1357
Bsu15I ATCGAT 2 cut(s) 135, 200
Bsu36I CCTNAGG 1 cut(s) 310
BsuI GTATCC 1 cut(s) 56
BsuRI GGCC 6 cut(s) 150, 1099, 1144, 1196, 1570, 1678
BsuTUI ATCGAT 2 cut(s) 135, 200
BtgI CCRYGG 1 cut(s) 1565
BtsCI GGATG 5 cut(s) 47, 180, 1468, 1486, 1623
BtsI GCAGTG 2 cut(s) 114, 1332
BtsIMutI CAGTG 6 cut(s) 75, 114, 419, 1152, 1313, 1332
BveI ACCTGC 2 cut(s) 493, 1044
Cac8I GCNNGC 1 cut(s) 1572
CaiI CAGNNNCTG 1 cut(s) 1151
CfoI GCGC 2 cut(s) 7, 225
Cfr10I RCCGGY 2 cut(s) 598, 1021
Cfr13I GGNCC 6 cut(s) 237, 1046, 1097, 1098, 1610, 1676
ClaI ATCGAT 2 cut(s) 135, 200
Csp6I GTAC 3 cut(s) 94, 440, 1619
CspAI ACCGGT 1 cut(s) 1021
CspCI CAANNNNNGTGG 2 cut(s) 1704, 1739
CviAII CATG 2 cut(s) 935, 1325
CviQI GTAC 3 cut(s) 94, 440, 1619
DdeI CTNAG 6 cut(s) 310, 340, 372, 432, 1473, 1551
DpnI GATC 5 cut(s) 869, 1251, 1284, 1359, 1486
DpnII GATC 5 cut(s) 867, 1249, 1282, 1357, 1484
EaeI YGGCCR 1 cut(s) 1194
Eam1104I CTCTTC 5 cut(s) 323, 597, 1227, 1424, 1788
EarI CTCTTC 5 cut(s) 323, 597, 1227, 1424, 1788
EciI GGCGGA 1 cut(s) 1582
Eco130I CCWWGG 2 cut(s) 1007, 1042
Eco24I GRGCYC 2 cut(s) 1101, 1381
Eco31I GGTCTC 1 cut(s) 320
Eco32I GATATC 1 cut(s) 1743
Eco47I GGWCC 3 cut(s) 237, 1046, 1610
Eco81I CCTNAGG 1 cut(s) 310
EcoO109I RGGNCCY 2 cut(s) 1097, 1610
EcoRII CCWGG 3 cut(s) 913, 1489, 1603
EcoRV GATATC 1 cut(s) 1743
EcoT14I CCWWGG 2 cut(s) 1007, 1042
EcoT38I GRGCYC 2 cut(s) 1101, 1381
ErhI CCWWGG 2 cut(s) 1007, 1042
FaeI CATG 2 cut(s) 938, 1328
FaqI GGGAC 2 cut(s) 452, 896
FatI CATG 2 cut(s) 934, 1324
FbaI TGATCA 1 cut(s) 867
Fnu4HI GCNGC 6 cut(s) 30, 151, 363, 489, 719, 1004
FokI GGATG 5 cut(s) 34, 167, 1455, 1493, 1610
FriOI GRGCYC 2 cut(s) 1101, 1381
Fsp4HI GCNGC 6 cut(s) 30, 151, 363, 489, 719, 1004
FspBI CTAG 3 cut(s) 741, 1119, 1721
GlaI GCGC 2 cut(s) 6, 224
GluI GCNGC 6 cut(s) 30, 151, 363, 489, 719, 1004
GsuI CTGGAG 2 cut(s) 1395, 1626
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 150, 1099, 1144, 1196, 1570, 1678
HapII CCGG 8 cut(s) 23, 168, 599, 1022, 1449, 1509, 1616, 1691
HhaI GCGC 2 cut(s) 7, 225
Hin1II CATG 2 cut(s) 938, 1328
Hin6I GCGC 2 cut(s) 5, 223
HinP1I GCGC 2 cut(s) 5, 223
HincII GTYRAC 4 cut(s) 294, 1114, 1654, 1770
HindII GTYRAC 4 cut(s) 294, 1114, 1654, 1770
HindIII AAGCTT 1 cut(s) 1637
HinfI GANTC 9 cut(s) 107, 231, 909, 924, 1274, 1346, 1428, 1512, 1687
HpaII CCGG 8 cut(s) 23, 168, 599, 1022, 1449, 1509, 1616, 1691
HphI GGTGA 2 cut(s) 1207, 1479
Hpy166II GTNNAC 7 cut(s) 294, 1049, 1114, 1582, 1600, 1654, 1770
Hpy188I TCNGA 7 cut(s) 241, 268, 435, 679, 1351, 1554, 1644
Hpy188III TCNNGA 7 cut(s) 23, 281, 928, 1271, 1423, 1472, 1509
Hpy8I GTNNAC 7 cut(s) 294, 1049, 1114, 1582, 1600, 1654, 1770
Hpy99I CGWCG 2 cut(s) 245, 1272
HpyAV CCTTC 4 cut(s) 684, 760, 1166, 1726
HpyCH4IV ACGT 2 cut(s) 243, 1110
HpyCH4V TGCA 9 cut(s) 41, 47, 362, 419, 491, 734, 789, 1332, 1574
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 26, 38, 494, 1009, 1182
HpyF3I CTNAG 6 cut(s) 310, 340, 372, 432, 1473, 1551
HpySE526I ACGT 2 cut(s) 243, 1110
Hsp92II CATG 2 cut(s) 938, 1328
HspAI GCGC 2 cut(s) 5, 223
Kpn2I TCCGGA 2 cut(s) 22, 1508
KpnI GGTACC 1 cut(s) 1622
Ksp22I TGATCA 1 cut(s) 867
Kzo9I GATC 5 cut(s) 867, 1249, 1282, 1357, 1484
LmnI GCTCC 5 cut(s) 19, 25, 715, 856, 1376
Lsp1109I GCAGC 5 cut(s) 16, 374, 475, 730, 990
LweI GCATC 4 cut(s) 56, 349, 640, 1477
MaeI CTAG 3 cut(s) 741, 1119, 1721
MaeII ACGT 2 cut(s) 243, 1110
MaeIII GTNAC 6 cut(s) 686, 837, 1031, 1147, 1369, 1383
MalI GATC 5 cut(s) 869, 1251, 1284, 1359, 1486
MboI GATC 5 cut(s) 867, 1249, 1282, 1357, 1484
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 1 cut(s) 1357
MhlI GDGCHC 3 cut(s) 1021, 1101, 1381
MlsI TGGCCA 1 cut(s) 1196
MluCI AATT 5 cut(s) 42, 322, 888, 1396, 1646
MluNI TGGCCA 1 cut(s) 1196
MlyI GAGTC 6 cut(s) 116, 918, 1355, 1422, 1506, 1681
MmeI TCCRAC 5 cut(s) 264, 280, 834, 1261, 1779
Mox20I TGGCCA 1 cut(s) 1196
MroI TCCGGA 2 cut(s) 22, 1508
MroXI GAANNNNTTC 1 cut(s) 1316
MscI TGGCCA 1 cut(s) 1196
MseI TTAA 6 cut(s) 9, 204, 459, 725, 779, 1091
MslI CAYNNNNRTG 1 cut(s) 475
Msp20I TGGCCA 1 cut(s) 1196
MspA1I CMGCKG 3 cut(s) 721, 1003, 1054
MspCI CTTAAG 1 cut(s) 8
MspI CCGG 8 cut(s) 23, 168, 599, 1022, 1449, 1509, 1616, 1691
MspR9I CCNGG 5 cut(s) 168, 915, 1491, 1605, 1692
MunI CAATTG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 3 cut(s) 915, 1491, 1605
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 6 cut(s) 11, 26, 38, 494, 1009, 1182
NciI CCSGG 2 cut(s) 168, 1692
NdeII GATC 5 cut(s) 867, 1249, 1282, 1357, 1484
NlaIII CATG 2 cut(s) 938, 1328
NlaIV GGNNCC 8 cut(s) 15, 483, 858, 1020, 1098, 1099, 1378, 1620
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 2 cut(s) 1031, 1147
PasI CCCWGGG 1 cut(s) 914
PctI GAATGC 1 cut(s) 580
PdmI GAANNNNTTC 1 cut(s) 1316
PfeI GAWTC 3 cut(s) 231, 924, 1274
PflMI CCANNNNNTGG 1 cut(s) 1151
PinAI ACCGGT 1 cut(s) 1021
PkrI GCNGC 6 cut(s) 31, 152, 364, 490, 720, 1005
PleI GAGTC 6 cut(s) 115, 917, 1354, 1422, 1506, 1681
PpsI GAGTC 6 cut(s) 115, 917, 1354, 1422, 1506, 1681
PpuMI RGGWCCY 1 cut(s) 1610
Psp5II RGGWCCY 1 cut(s) 1610
Psp6I CCWGG 3 cut(s) 913, 1489, 1603
PspGI CCWGG 3 cut(s) 913, 1489, 1603
PspN4I GGNNCC 8 cut(s) 15, 483, 858, 1020, 1098, 1099, 1378, 1620
PspOMI GGGCCC 1 cut(s) 1097
PspPI GGNCC 6 cut(s) 237, 1046, 1097, 1098, 1610, 1676
PspPPI RGGWCCY 1 cut(s) 1610
PstI CTGCAG 1 cut(s) 1576
PstNI CAGNNNCTG 1 cut(s) 1151
PsuI RGATCY 1 cut(s) 1357
PvuII CAGCTG 1 cut(s) 1003
RsaI GTAC 3 cut(s) 95, 441, 1620
RsaNI GTAC 3 cut(s) 94, 440, 1619
RseI CAYNNNNRTG 1 cut(s) 475
SaqAI TTAA 6 cut(s) 9, 204, 459, 725, 779, 1091
SatI GCNGC 6 cut(s) 30, 151, 363, 489, 719, 1004
Sau3AI GATC 5 cut(s) 867, 1249, 1282, 1357, 1484
Sau96I GGNCC 6 cut(s) 237, 1046, 1097, 1098, 1610, 1676
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 6 cut(s) 116, 918, 1355, 1422, 1506, 1681
ScrFI CCNGG 5 cut(s) 168, 915, 1491, 1605, 1692
SduI GDGCHC 3 cut(s) 1021, 1101, 1381
SfaNI GCATC 4 cut(s) 56, 349, 640, 1477
SfcI CTRYAG 2 cut(s) 1079, 1572
SinI GGWCC 3 cut(s) 237, 1046, 1610
SmiMI CAYNNNNRTG 1 cut(s) 475
SmlI CTYRAG 4 cut(s) 8, 89, 928, 1423
SmoI CTYRAG 4 cut(s) 8, 89, 928, 1423
SpeI ACTAGT 2 cut(s) 740, 1118
Sse9I AATT 5 cut(s) 42, 322, 888, 1396, 1646
SsiI CCGC 5 cut(s) 151, 721, 1054, 1432, 1593
SspI AATATT 1 cut(s) 1779
SspMI CTAG 3 cut(s) 741, 1119, 1721
StyD4I CCNGG 5 cut(s) 166, 913, 1489, 1603, 1690
StyI CCWWGG 2 cut(s) 1007, 1042
TaiI ACGT 2 cut(s) 246, 1113
TaqI TCGA 4 cut(s) 121, 135, 200, 1242
TasI AATT 5 cut(s) 42, 322, 888, 1396, 1646
TatI WGTACW 1 cut(s) 93
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 3 cut(s) 231, 924, 1274
Tru1I TTAA 6 cut(s) 9, 204, 459, 725, 779, 1091
Tru9I TTAA 6 cut(s) 9, 204, 459, 725, 779, 1091
TscAI CASTG 6 cut(s) 82, 121, 426, 1152, 1313, 1339
TseFI GTSAC 2 cut(s) 1031, 1147
TseI GCWGC 5 cut(s) 29, 362, 488, 718, 1003
Tsp45I GTSAC 2 cut(s) 1031, 1147
TspDTI ATGAA 3 cut(s) 388, 584, 846
TspGWI ACGGA 1 cut(s) 1739
TspRI CASTG 6 cut(s) 82, 121, 426, 1152, 1313, 1339
Van91I CCANNNNNTGG 1 cut(s) 1151
Vha464I CTTAAG 1 cut(s) 8
VpaK11BI GGWCC 3 cut(s) 237, 1046, 1610
XapI RAATTY 1 cut(s) 1646
XmnI GAANNNNTTC 1 cut(s) 1316
XspI CTAG 3 cut(s) 741, 1119, 1721
ZrmI AGTACT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.