FvH4_5g28650

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007444
Physical Location & Seq
Reverse (-)
19836161 .. 19841904
5744 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28650.t1

Sequence Viewer

Length: 1737 bp
ATGGCGCTCAAGGTCGGAGCTCCACAAGCTGCTGAGATTGCGATTGCATCAATTGGGCGTGGATACGATGTCTCCATTGATTTAAGGCTCAAGTACTGCAAAGGCGAGTCTCGATTGATTGAAATCGACGACGACGGCGGCATCAAATGCGATAAAGGAGAGCGCACCCGTTTCAGGTCCGATGTTCTCTCTTTCCAACAGATGTCGGAGCATTTCAACCAGGAAGTTGGTTTGACCGGCAAAATACCCTCGGGCCTCTTCAATTCTATGTTTGAATTCTCAGGTTGTTGGCAAAAAGATGCAGCCAACACTAAGACCCTTGCCTTCGACGGTGTCTTCATCACGCTCTATTCTGTCGCCCTCGACAAGTCCCACATGCTACTTCGTGATCATGTTAAAAAGGCAGTCCCGTCCTCATGGGAGCCTGCTACATTAGCCAGGTTTATAGAGACATTCGGAACGCATATAGTTGTTGGTGTCAAGATGGGAGGTAAGGATGTCATCTTTATGAAGCAACAGCACTCTTCCACTCTTCAACCGGCAGATATACAGAAAAGATTAAAGGACATGGCGGATAAAAGATTTTTGGATGGCATACCGTCTGCTCAAGCTTACAAGAATGACAAGTTTGAAATCAGGGAGCAGAGGTTGCGGTTTGCAGATAGTAGTCCATCAAGTTCTTATTCACACAAGGAAGATATTGTAAGCATTCGCAAAAGGAGAGGCGGCAGTGATATTAAGAACCTGTCCCATAATGAATGGTTACAAAGTGTTCAACTTGAACCTGATGTGATCACAATGTCATTTATACCAATCACCTCTCTTTTAAATGGAGTCCCCGGGAGTGGATTCTTGAGCCATGCAATAAATCTTTATTTAAGATATAAACCACCAATTGAAGAGCTACATCAGTTTCTGGAATTCCAGCTGCCAAGGCAGTGGGCGCCAGTTTTTAGCGACCTTCCTCTTGGTCCACAAAGGAAGAACCAAAGCAATACATCTCTACAGTTTAGCTTAATGGGGCCCAAGCTATATGTCAATACTAGTCTGGTTGATGTAGGAAAGAGACCGGTGACTGGTCTGCGGCTGTATCTAGAAGGCAAAAGAAGCAACCGCTTGGCCATCCACTTACAACACCTCTCCTCTCTTCCAAAAATCTTCCAACTCGAAGATGATCCAAATGGAAATTTCCATCATGATTCTTATGATCGTAAATACTATGAGAAAGTCCAATGGAAGAACTTCTCTCATGTGTGCACTGCTCCTGTGGAGTCGGATGAGGATCTTTCAGTAGTAACTGGGGCGCAGTTGCAGGTTGAGAATTATGGGATAAAAAATATTCTCTCCTTGAGACTCCGCTTCTCTACTGTTTCCGGAGCTACAATCGTAAAGCATCCTGAATGGGATGGGTCACCAGGGTTGGCCCATAAGTCGGGACTGATATCAACACTGATCAGTCATCACTTTACAACAGTTCAAAAGCCACCCCCACGACCAGCAGATGTCAACATAAACTCTGCTGTCTACCCTGGGGGCCCTCCAGTTCCCATCCAAGCCCCGAGACTCAGGAAATTTGTGGATACAACAGAAATGACAAGGGGGCCAGAGGAAACTCCAGGTTATTGGGTTGTATCTGGGGCAAGACTAGTTGTCGAAAAGGGCAGGATATCACTGCGTGCGAAGTATTCTTTATTGACTGTAATATTACCTGATGAAGAAGAGTTGGAGGAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

579

Amino Acids

64.89

Weight (kDa)

8.72

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 86 - 292 2e-40 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1303
AccB1I GGYRCC 1 cut(s) 943
AccI GTMKAC 1 cut(s) 1524
AccIII TCCGGA 1 cut(s) 1373
AciI CCGC 7 cut(s) 138, 572, 652, 726, 1084, 1114, 1357
AclWI GGATC 2 cut(s) 1169, 1290
AcoI YGGCCR 1 cut(s) 1119
AcsI RAATTY 4 cut(s) 275, 920, 1186, 1571
AcyI GRCGYC 1 cut(s) 944
AdeI CACNNNGTG 1 cut(s) 1676
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 4 cut(s) 174, 845, 1076, 1432
AgeI ACCGGT 1 cut(s) 1069
AhdI GACNNNNNGTC 1 cut(s) 1649
AhlI ACTAGT 2 cut(s) 1043, 1645
AjnI CCWGG 5 cut(s) 219, 437, 1414, 1528, 1615
AluBI AGCT 8 cut(s) 20, 29, 611, 904, 928, 1014, 1030, 1379
AluI AGCT 8 cut(s) 20, 29, 611, 904, 928, 1014, 1030, 1379
Alw21I GWGCWC 2 cut(s) 22, 1259
Alw26I GTCTC 6 cut(s) 76, 114, 443, 1060, 1345, 1555
Alw44I GTGCAC 1 cut(s) 1255
AlwI GGATC 2 cut(s) 1169, 1290
AlwNI CAGNNNCTG 1 cut(s) 916
Ama87I CYCGRG 3 cut(s) 250, 839, 1558
Aor13HI TCCGGA 1 cut(s) 1373
AoxI GGCC 6 cut(s) 253, 1022, 1119, 1422, 1534, 1601
ApaI GGGCCC 2 cut(s) 1026, 1538
ApaLI GTGCAC 1 cut(s) 1255
ApeKI GCWGC 3 cut(s) 29, 302, 928
ApoI RAATTY 4 cut(s) 275, 920, 1186, 1571
AsiGI ACCGGT 1 cut(s) 1069
Asp700I GAANNNNTTC 1 cut(s) 1241
AspLEI GCGC 4 cut(s) 7, 165, 946, 1306
AspS9I GGNCC 9 cut(s) 177, 253, 971, 1022, 1023, 1423, 1534, 1535, 1601
AsuC2I CCSGG 2 cut(s) 840, 841
AsuHPI GGTGA 3 cut(s) 808, 1084, 1404
AvaI CYCGRG 3 cut(s) 250, 839, 1558
AvaII GGWCC 2 cut(s) 177, 971
BaeGI GKGCMC 3 cut(s) 1026, 1259, 1538
BalI TGGCCA 1 cut(s) 1121
BanI GGYRCC 1 cut(s) 943
BanII GRGCYC 3 cut(s) 22, 1026, 1538
BbsI GAAGAC 1 cut(s) 328
Bbv12I GWGCWC 2 cut(s) 22, 1259
BbvI GCAGC 3 cut(s) 16, 314, 915
BccI CCATC 7 cut(s) 478, 584, 679, 1130, 1200, 1400, 1556
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 5 cut(s) 221, 439, 1416, 1530, 1617
BciVI GTATCC 2 cut(s) 56, 1573
BclI TGATCA 3 cut(s) 388, 792, 1452
BcnI CCSGG 2 cut(s) 840, 841
BcoDI GTCTC 6 cut(s) 76, 114, 443, 1060, 1345, 1555
BcuI ACTAGT 2 cut(s) 1043, 1645
BfaI CTAG 3 cut(s) 1044, 1094, 1646
BfmI CTRYAG 1 cut(s) 1004
BfoI RGCGCY 2 cut(s) 8, 947
BfuAI ACCTGC 1 cut(s) 1303
BfuI GTATCC 2 cut(s) 56, 1573
BisI GCNGC 6 cut(s) 30, 139, 303, 727, 929, 1085
BlsI GCNGC 6 cut(s) 31, 140, 304, 728, 930, 1086
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 7 cut(s) 221, 439, 840, 841, 1416, 1530, 1617
Bme18I GGWCC 2 cut(s) 177, 971
BmeRI GACNNNNNGTC 1 cut(s) 1649
BmeT110I CYCGRG 3 cut(s) 250, 839, 1558
BmgT120I GGNCC 9 cut(s) 177, 253, 971, 1022, 1023, 1423, 1534, 1535, 1601
BmiI GGNNCC 7 cut(s) 423, 945, 1023, 1024, 1535, 1536, 1602
BmrFI CCNGG 7 cut(s) 221, 439, 840, 841, 1416, 1530, 1617
BmrI ACTGGG 1 cut(s) 1308
BmsI GCATC 4 cut(s) 56, 150, 289, 1402
BmuI ACTGGG 1 cut(s) 1308
BpiI GAAGAC 1 cut(s) 328
BpmI CTGGAG 2 cut(s) 1524, 1599
BpuEI CTTGAG 4 cut(s) 74, 591, 874, 1369
BpuMI CCSGG 2 cut(s) 840, 841
BsaBI GATNNNNATC 2 cut(s) 122, 1281
BsaHI GRCGYC 1 cut(s) 944
BsaI GGTCTC 1 cut(s) 1060
BsaJI CCNNGG 7 cut(s) 249, 838, 839, 932, 1415, 1528, 1529
BsaWI WCCGGW 2 cut(s) 1069, 1373
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 632, 662
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 845, 1076, 1432
Bse118I RCCGGY 3 cut(s) 236, 538, 1069
Bse1I ACTGG 4 cut(s) 947, 1081, 1303, 1541
Bse8I GATNNNNATC 2 cut(s) 122, 1281
BseAI TCCGGA 1 cut(s) 1373
BseBI CCWGG 5 cut(s) 221, 439, 1416, 1530, 1617
BseDI CCNNGG 7 cut(s) 249, 838, 839, 932, 1415, 1528, 1529
BseGI GGATG 7 cut(s) 502, 595, 1122, 1282, 1393, 1411, 1548
BseJI GATNNNNATC 2 cut(s) 122, 1281
BseLI CCNNNNNNNGG 4 cut(s) 174, 845, 1076, 1432
BseMII CTCAG 3 cut(s) 24, 294, 1579
BseNI ACTGG 4 cut(s) 947, 1081, 1303, 1541
BseRI GAGGAG 1 cut(s) 1132
BseSI GKGCMC 3 cut(s) 1026, 1259, 1538
BseXI GCAGC 3 cut(s) 16, 314, 915
BshFI GGCC 6 cut(s) 255, 1024, 1121, 1424, 1536, 1603
BshNI GGYRCC 1 cut(s) 943
BshTI ACCGGT 1 cut(s) 1069
BsiHKAI GWGCWC 2 cut(s) 22, 1259
BsiHKCI CYCGRG 3 cut(s) 250, 839, 1558
BsiSI CCGG 5 cut(s) 237, 539, 840, 1070, 1374
BslFI GGGAC 5 cut(s) 355, 392, 733, 821, 1449
BslI CCNNNNNNNGG 4 cut(s) 174, 845, 1076, 1432
BsmAI GTCTC 6 cut(s) 76, 114, 443, 1060, 1345, 1555
BsmFI GGGAC 5 cut(s) 355, 392, 733, 821, 1449
BsmI GAATGC 1 cut(s) 708
BsnI GGCC 6 cut(s) 255, 1024, 1121, 1424, 1536, 1603
Bso31I GGTCTC 1 cut(s) 1060
BsoBI CYCGRG 3 cut(s) 250, 839, 1558
Bsp120I GGGCCC 2 cut(s) 1022, 1534
Bsp1286I GDGCHC 4 cut(s) 22, 1026, 1259, 1538
Bsp13I TCCGGA 1 cut(s) 1373
Bsp143I GATC 6 cut(s) 388, 792, 1174, 1207, 1282, 1452
BspACI CCGC 7 cut(s) 138, 572, 652, 726, 1084, 1114, 1357
BspANI GGCC 6 cut(s) 255, 1024, 1121, 1424, 1536, 1603
BspCNI CTCAG 3 cut(s) 25, 293, 1578
BspEI TCCGGA 1 cut(s) 1373
BspHI TCATGA 1 cut(s) 1195
BspLI GGNNCC 7 cut(s) 423, 945, 1023, 1024, 1535, 1536, 1602
BspMI ACCTGC 1 cut(s) 1303
BspPI GGATC 2 cut(s) 1169, 1290
BspQI GCTCTTC 1 cut(s) 894
BspT107I GGYRCC 1 cut(s) 943
BspTNI GGTCTC 1 cut(s) 1060
BsrFI RCCGGY 3 cut(s) 236, 538, 1069
BsrI ACTGG 4 cut(s) 947, 1081, 1303, 1541
BssAI RCCGGY 3 cut(s) 236, 538, 1069
BssECI CCNNGG 7 cut(s) 249, 838, 839, 932, 1415, 1528, 1529
BssMI GATC 6 cut(s) 388, 792, 1174, 1207, 1282, 1452
BssNI GRCGYC 1 cut(s) 944
BssT1I CCWWGG 1 cut(s) 932
Bst2UI CCWGG 5 cut(s) 221, 439, 1416, 1530, 1617
Bst4CI ACNGT 6 cut(s) 332, 600, 1008, 1369, 1474, 1699
Bst6I CTCTTC 6 cut(s) 263, 529, 537, 894, 1152, 1713
BstACI GRCGYC 1 cut(s) 944
BstAPI GCANNNNNTGC 2 cut(s) 147, 649
BstC8I GCNNGC 2 cut(s) 426, 1677
BstDEI CTNAG 4 cut(s) 33, 280, 312, 1565
BstEII GGTNACC 1 cut(s) 1410
BstF5I GGATG 7 cut(s) 502, 595, 1122, 1282, 1393, 1411, 1548
BstH2I RGCGCY 2 cut(s) 8, 947
BstHHI GCGC 4 cut(s) 7, 165, 946, 1306
BstKTI GATC 6 cut(s) 391, 795, 1177, 1210, 1285, 1455
BstMAI GTCTC 6 cut(s) 76, 114, 443, 1060, 1345, 1555
BstMBI GATC 6 cut(s) 388, 792, 1174, 1207, 1282, 1452
BstMWI GCNNNNNNNGC 8 cut(s) 26, 38, 147, 434, 649, 934, 943, 1107
BstNI CCWGG 5 cut(s) 221, 439, 1416, 1530, 1617
BstNSI RCATGY 1 cut(s) 379
BstPI GGTNACC 1 cut(s) 1410
BstSCI CCNGG 7 cut(s) 219, 437, 838, 839, 1414, 1528, 1615
BstSFI CTRYAG 1 cut(s) 1004
BstSLI GKGCMC 3 cut(s) 1026, 1259, 1538
BstV1I GCAGC 3 cut(s) 16, 314, 915
BstV2I GAAGAC 1 cut(s) 328
BstX2I RGATCY 1 cut(s) 1282
BstXI CCANNNNNNTGG 3 cut(s) 227, 939, 1623
BstYI RGATCY 1 cut(s) 1282
BsuI GTATCC 2 cut(s) 56, 1573
BsuRI GGCC 6 cut(s) 255, 1024, 1121, 1424, 1536, 1603
BtsCI GGATG 7 cut(s) 502, 595, 1122, 1282, 1393, 1411, 1548
BtsI GCAGTG 4 cut(s) 736, 944, 1257, 1670
BtsIMutI CAGTG 5 cut(s) 736, 944, 1257, 1448, 1670
BveI ACCTGC 1 cut(s) 1303
Cac8I GCNNGC 2 cut(s) 426, 1677
CaiI CAGNNNCTG 1 cut(s) 916
CciI TCATGA 1 cut(s) 1195
CfoI GCGC 4 cut(s) 7, 165, 946, 1306
Cfr10I RCCGGY 3 cut(s) 236, 538, 1069
Cfr13I GGNCC 9 cut(s) 177, 253, 971, 1022, 1023, 1423, 1534, 1535, 1601
Cfr9I CCCGGG 1 cut(s) 839
Csp6I GTAC 1 cut(s) 94
CspAI ACCGGT 1 cut(s) 1069
CviAII CATG 7 cut(s) 376, 392, 417, 568, 860, 1196, 1250
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 4 cut(s) 33, 280, 312, 1565
DinI GGCGCC 1 cut(s) 945
DpnI GATC 6 cut(s) 390, 794, 1176, 1209, 1284, 1454
DpnII GATC 6 cut(s) 388, 792, 1174, 1207, 1282, 1452
DraI TTTAAA 1 cut(s) 828
DraIII CACNNNGTG 1 cut(s) 1676
DriI GACNNNNNGTC 1 cut(s) 1649
EaeI YGGCCR 1 cut(s) 1119
Eam1104I CTCTTC 6 cut(s) 263, 529, 537, 894, 1152, 1713
Eam1105I GACNNNNNGTC 1 cut(s) 1649
EarI CTCTTC 6 cut(s) 263, 529, 537, 894, 1152, 1713
EciI GGCGGA 1 cut(s) 587
Ecl136II GAGCTC 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 932
Eco24I GRGCYC 3 cut(s) 22, 1026, 1538
Eco31I GGTCTC 1 cut(s) 1060
Eco32I GATATC 2 cut(s) 1443, 1668
Eco47I GGWCC 2 cut(s) 177, 971
Eco53kI GAGCTC 1 cut(s) 20
Eco88I CYCGRG 3 cut(s) 250, 839, 1558
Eco91I GGTNACC 1 cut(s) 1410
EcoICRI GAGCTC 1 cut(s) 20
EcoO109I RGGNCCY 3 cut(s) 1022, 1534, 1535
EcoO65I GGTNACC 1 cut(s) 1410
EcoRI GAATTC 2 cut(s) 275, 920
EcoRII CCWGG 5 cut(s) 219, 437, 1414, 1528, 1615
EcoRV GATATC 2 cut(s) 1443, 1668
EcoT14I CCWWGG 1 cut(s) 932
EcoT38I GRGCYC 3 cut(s) 22, 1026, 1538
EgeI GGCGCC 1 cut(s) 945
EheI GGCGCC 1 cut(s) 945
ErhI CCWWGG 1 cut(s) 932
FaeI CATG 7 cut(s) 379, 395, 420, 571, 863, 1199, 1253
FaqI GGGAC 5 cut(s) 355, 392, 733, 821, 1449
FatI CATG 7 cut(s) 375, 391, 416, 567, 859, 1195, 1249
FbaI TGATCA 3 cut(s) 388, 792, 1452
FblI GTMKAC 1 cut(s) 1524
Fnu4HI GCNGC 6 cut(s) 30, 139, 303, 727, 929, 1085
FokI GGATG 7 cut(s) 509, 602, 1109, 1289, 1380, 1418, 1535
FriOI GRGCYC 3 cut(s) 22, 1026, 1538
Fsp4HI GCNGC 6 cut(s) 30, 139, 303, 727, 929, 1085
FspBI CTAG 3 cut(s) 1044, 1094, 1646
GlaI GCGC 4 cut(s) 6, 164, 945, 1305
GluI GCNGC 6 cut(s) 30, 139, 303, 727, 929, 1085
GsuI CTGGAG 2 cut(s) 1524, 1599
HaeII RGCGCY 2 cut(s) 8, 947
HaeIII GGCC 6 cut(s) 255, 1024, 1121, 1424, 1536, 1603
HapII CCGG 5 cut(s) 237, 539, 840, 1070, 1374
HhaI GCGC 4 cut(s) 7, 165, 946, 1306
Hin1I GRCGYC 1 cut(s) 944
Hin1II CATG 7 cut(s) 379, 395, 420, 571, 863, 1199, 1253
Hin6I GCGC 4 cut(s) 5, 163, 944, 1304
HinP1I GCGC 4 cut(s) 5, 163, 944, 1304
HincII GTYRAC 1 cut(s) 1507
HindII GTYRAC 1 cut(s) 1507
HindIII AAGCTT 1 cut(s) 609
HinfI GANTC 7 cut(s) 107, 834, 849, 1199, 1271, 1353, 1563
HpaII CCGG 5 cut(s) 237, 539, 840, 1070, 1374
HphI GGTGA 3 cut(s) 808, 1084, 1404
Hpy166II GTNNAC 4 cut(s) 974, 1257, 1507, 1525
Hpy188I TCNGA 5 cut(s) 17, 181, 208, 458, 1276
Hpy8I GTNNAC 4 cut(s) 974, 1257, 1507, 1525
Hpy99I CGWCG 4 cut(s) 131, 134, 137, 332
HpyAV CCTTC 3 cut(s) 334, 971, 1091
HpyCH4III ACNGT 6 cut(s) 332, 600, 1008, 1369, 1474, 1699
HpyCH4V TGCA 7 cut(s) 47, 99, 302, 659, 863, 1257, 1312
HpyF10VI GCNNNNNNNGC 8 cut(s) 26, 38, 147, 434, 649, 934, 943, 1107
HpyF3I CTNAG 4 cut(s) 33, 280, 312, 1565
Hsp92I GRCGYC 1 cut(s) 944
Hsp92II CATG 7 cut(s) 379, 395, 420, 571, 863, 1199, 1253
HspAI GCGC 4 cut(s) 5, 163, 944, 1304
KasI GGCGCC 1 cut(s) 943
Kpn2I TCCGGA 1 cut(s) 1373
Ksp22I TGATCA 3 cut(s) 388, 792, 1452
Kzo9I GATC 6 cut(s) 388, 792, 1174, 1207, 1282, 1452
LguI GCTCTTC 1 cut(s) 894
LmnI GCTCC 7 cut(s) 17, 25, 208, 421, 640, 1267, 1376
Lsp1109I GCAGC 3 cut(s) 16, 314, 915
LweI GCATC 4 cut(s) 56, 150, 289, 1402
MaeI CTAG 3 cut(s) 1044, 1094, 1646
MaeIII GTNAC 4 cut(s) 762, 1072, 1294, 1410
MalI GATC 6 cut(s) 390, 794, 1176, 1209, 1284, 1454
MboI GATC 6 cut(s) 388, 792, 1174, 1207, 1282, 1452
MfeI CAATTG 2 cut(s) 51, 894
MflI RGATCY 1 cut(s) 1282
MhlI GDGCHC 4 cut(s) 22, 1026, 1259, 1538
MlsI TGGCCA 1 cut(s) 1121
MluCI AATT 8 cut(s) 51, 262, 275, 894, 920, 1186, 1321, 1571
MluNI TGGCCA 1 cut(s) 1121
Mly113I GGCGCC 1 cut(s) 944
MlyI GAGTC 5 cut(s) 116, 843, 1280, 1347, 1557
MmeI TCCRAC 5 cut(s) 186, 220, 1186, 1254, 1704
Mox20I TGGCCA 1 cut(s) 1121
MroI TCCGGA 1 cut(s) 1373
MroXI GAANNNNTTC 1 cut(s) 1241
MscI TGGCCA 1 cut(s) 1121
MseI TTAA 8 cut(s) 83, 396, 560, 738, 827, 878, 1016, 1735
MslI CAYNNNNRTG 1 cut(s) 506
Msp20I TGGCCA 1 cut(s) 1121
MspA1I CMGCKG 1 cut(s) 928
MspI CCGG 5 cut(s) 237, 539, 840, 1070, 1374
MspR9I CCNGG 7 cut(s) 221, 439, 840, 841, 1416, 1530, 1617
MunI CAATTG 2 cut(s) 51, 894
Mva1269I GAATGC 1 cut(s) 708
MvaI CCWGG 5 cut(s) 221, 439, 1416, 1530, 1617
MwoI GCNNNNNNNGC 8 cut(s) 26, 38, 147, 434, 649, 934, 943, 1107
NarI GGCGCC 1 cut(s) 944
NciI CCSGG 2 cut(s) 840, 841
NdeII GATC 6 cut(s) 388, 792, 1174, 1207, 1282, 1452
NlaIII CATG 7 cut(s) 379, 395, 420, 571, 863, 1199, 1253
NlaIV GGNNCC 7 cut(s) 423, 945, 1023, 1024, 1535, 1536, 1602
NmuCI GTSAC 2 cut(s) 1072, 1410
NspI RCATGY 1 cut(s) 379
PagI TCATGA 1 cut(s) 1195
PasI CCCWGGG 1 cut(s) 1529
PciSI GCTCTTC 1 cut(s) 894
PctI GAATGC 1 cut(s) 708
PdmI GAANNNNTTC 1 cut(s) 1241
PfeI GAWTC 2 cut(s) 849, 1199
PflFI GACNNNGTC 1 cut(s) 332
PinAI ACCGGT 1 cut(s) 1069
PkrI GCNGC 6 cut(s) 31, 140, 304, 728, 930, 1086
PleI GAGTC 5 cut(s) 115, 842, 1279, 1347, 1557
PluTI GGCGCC 1 cut(s) 947
PpsI GAGTC 5 cut(s) 115, 842, 1279, 1347, 1557
Psp124BI GAGCTC 1 cut(s) 22
Psp6I CCWGG 5 cut(s) 219, 437, 1414, 1528, 1615
PspEI GGTNACC 1 cut(s) 1410
PspGI CCWGG 5 cut(s) 219, 437, 1414, 1528, 1615
PspN4I GGNNCC 7 cut(s) 423, 945, 1023, 1024, 1535, 1536, 1602
PspOMI GGGCCC 2 cut(s) 1022, 1534
PspPI GGNCC 9 cut(s) 177, 253, 971, 1022, 1023, 1423, 1534, 1535, 1601
PstNI CAGNNNCTG 1 cut(s) 916
PsuI RGATCY 1 cut(s) 1282
PsyI GACNNNGTC 1 cut(s) 332
PvuII CAGCTG 1 cut(s) 928
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 1 cut(s) 506
SacI GAGCTC 1 cut(s) 22
SapI GCTCTTC 1 cut(s) 894
SaqAI TTAA 8 cut(s) 83, 396, 560, 738, 827, 878, 1016, 1735
SatI GCNGC 6 cut(s) 30, 139, 303, 727, 929, 1085
Sau3AI GATC 6 cut(s) 388, 792, 1174, 1207, 1282, 1452
Sau96I GGNCC 9 cut(s) 177, 253, 971, 1022, 1023, 1423, 1534, 1535, 1601
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 5 cut(s) 116, 843, 1280, 1347, 1557
ScrFI CCNGG 7 cut(s) 221, 439, 840, 841, 1416, 1530, 1617
SduI GDGCHC 4 cut(s) 22, 1026, 1259, 1538
SfaNI GCATC 4 cut(s) 56, 150, 289, 1402
SfcI CTRYAG 1 cut(s) 1004
SfoI GGCGCC 1 cut(s) 945
SinI GGWCC 2 cut(s) 177, 971
SmaI CCCGGG 1 cut(s) 841
SmiMI CAYNNNNRTG 1 cut(s) 506
SmlI CTYRAG 5 cut(s) 8, 89, 606, 853, 1348
SmoI CTYRAG 5 cut(s) 8, 89, 606, 853, 1348
SpeI ACTAGT 2 cut(s) 1043, 1645
Sse9I AATT 8 cut(s) 51, 262, 275, 894, 920, 1186, 1321, 1571
SsiI CCGC 7 cut(s) 138, 572, 652, 726, 1084, 1114, 1357
SspDI GGCGCC 1 cut(s) 943
SspI AATATT 2 cut(s) 1339, 1704
SspMI CTAG 3 cut(s) 1044, 1094, 1646
SstI GAGCTC 1 cut(s) 22
StyD4I CCNGG 7 cut(s) 219, 437, 838, 839, 1414, 1528, 1615
StyI CCWWGG 1 cut(s) 932
TaaI ACNGT 6 cut(s) 332, 600, 1008, 1369, 1474, 1699
TaqI TCGA 6 cut(s) 112, 126, 327, 363, 1167, 1653
TasI AATT 8 cut(s) 51, 262, 275, 894, 920, 1186, 1321, 1571
TatI WGTACW 1 cut(s) 93
TauI GCSGC 3 cut(s) 141, 729, 1087
TfiI GAWTC 2 cut(s) 849, 1199
Tru1I TTAA 8 cut(s) 83, 396, 560, 738, 827, 878, 1016, 1735
Tru9I TTAA 8 cut(s) 83, 396, 560, 738, 827, 878, 1016, 1735
TscAI CASTG 5 cut(s) 736, 944, 1264, 1455, 1677
TseFI GTSAC 2 cut(s) 1072, 1410
TseI GCWGC 3 cut(s) 29, 302, 928
Tsp45I GTSAC 2 cut(s) 1072, 1410
TspDTI ATGAA 4 cut(s) 328, 524, 771, 1728
TspMI CCCGGG 1 cut(s) 839
TspRI CASTG 5 cut(s) 736, 944, 1264, 1455, 1677
Tth111I GACNNNGTC 1 cut(s) 332
VneI GTGCAC 1 cut(s) 1255
VpaK11BI GGWCC 2 cut(s) 177, 971
XapI RAATTY 4 cut(s) 275, 920, 1186, 1571
XbaI TCTAGA 1 cut(s) 1093
XceI RCATGY 1 cut(s) 379
XmaI CCCGGG 1 cut(s) 839
XmiI GTMKAC 1 cut(s) 1524
XmnI GAANNNNTTC 1 cut(s) 1241
XspI CTAG 3 cut(s) 1044, 1094, 1646
ZrmI AGTACT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.