Rroxscaffold_3G00239260

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007444
Physical Location & Seq
Reverse (-)
29222984 .. 29228266
5283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00239260.1

Sequence Viewer

Length: 1677 bp
ATGGCGCTCAAGGTTGCAGCTCCAAAAGCTGCTGAAGCTGCTGAGGCCGCGATTGCATCAATTGGGTGTGGATATGATGTATCCATTGATCTAAGGCTCAAGTACTGCAAAGGGGACTCTCGATTGATTGAAATTGACGACGACGACGGGGGTCGCCAGGTTCTTCTTCCAGGTGGAATTACGATCCCCAACGTTTCCAAATCCATCAAATGCGATAAAGGAGAGCGCACGCGTTTCAGGTCCGATGTTCTCTCTTTCCAACAGATGTCAGAGCAGTTCAACCAGCAAGTTTCTTTGACCGGAAAAATACCCTCGGGTCTCTTCAATTCTATGTTTGAATTCTCAGGTTGTTGGCAGAAAGATGCAGCCAACACTAAGACCCTTGCTTTCGACGGGGTCTTCATCACACTCTATACTGTTGCTCTCGAAAAGTCTCAGATGGTACTACGTGATGATGTTAAAAAAGCAGTCCCATCGTCATGGGAACCTGCCGCATTAGCAAGGTTTATAGAGACATTTGGAACGCATGTAGTTGTTGGGGTGAAGATGGGAGGTAAAGATGTCATCTATATGAAGCAGCAGCATTCTTCAACTCTTCAACCAGCTGATATACAGAGAAGATTGAAGGACATGGCAGATAAAAGATTTTTAGATGCCAATGGACAGTACGGCATACCGTCTGAACAAGCTTACAAGAATGACAAGTTTGAAATCAGGGAGCAGCGGTTGCGGTTTGCTGATACTAGTCCATCTAGTTCTTATTCACACAAGGAATTGCAGGATATTGTAAGCATTCGCAAAAGGAGAGGTGGAAGTGATATTAAGAACCTATCCCATAATGAATGGTTACAAAGTGTTCAACATGAACCTGATGTGATCACAATGTCATTTATGCCAATTACCTCTCTGCTAAATGGAGTCCCAGGGAGTGGATTCTTGAGCCACGCAATAAATCTTTATTTAAGATATAAACCACCAATTGAAGAGCTCCATCAGTTTTTGGAATTTCAGCTGCCAAGGCAGTGGGCTCCCGTTTTCAGTGACCTTCCTCTTGGTCCACAAAGGAAGCATCAAAGCAATACATCTCTTCAGTTTAGCTTAATGGGGCCCAAGCTTTATGTCAATACTAGTCTGGTTGATGTAGGAAAGAGGCCAGTGACTGGTCTGCGGCTATATCTAGAAGGCAAAAGAAGCAACCGGTTGGCCATCCACTTACAACACCTCTCCTCTCTTCCGAAAATCTTCCAACTCGAAGATGATCCAAATGGAAATTTCCGTTGTGATTCTTATGATCGTAAATACTATGAGAAAGTCCAATGGAAGAACTTCTCTCATGTATGCACTGCGCCTGTGGAGTCGGATGAGGATCTTTCAATAGTAACTGGGGCACAGTTGCAGGTTGAGAAGTATGGGATAAAGAATATTCTCTTCTTGAGACTCCGCTTCTCTACCGTTTCCGGAGCTACAATTGTAAAGCATCCCGAATGGGATGGGTCACCAGGGTTGGGCCACAAGTCAGGACTCATATCAACACTAATCAGTCATCACTTTACAGCAGTTCAAAGGCCACCTCCACGACCAGCAGATGTCAACATAAACTCGGCTGTCTACCCCGGGGTCCTCCAGTTCCCATCCAAGCGCCAAAGCTCAGGAAATTTGTGGATACAACAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

558

Amino Acids

62.6

Weight (kDa)

9.14

Isoelectric Point (pI)

50.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 107 - 320 6.5e-41 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 496, 1387
AccB7I CCANNNNNTGG 2 cut(s) 929, 1160
AccI GTMKAC 1 cut(s) 1608
AccII CGCG 2 cut(s) 50, 232
AccIII TCCGGA 1 cut(s) 1457
AciI CCGC 6 cut(s) 48, 492, 724, 730, 1168, 1441
AclI AACGTT 1 cut(s) 192
AclWI GGATC 3 cut(s) 178, 1253, 1374
AcoI YGGCCR 1 cut(s) 1203
AcsI RAATTY 4 cut(s) 338, 1004, 1270, 1654
AcuI CTGAAG 2 cut(s) 54, 1073
AfaI GTAC 3 cut(s) 104, 444, 668
AfiI CCNNNNNNNGG 3 cut(s) 929, 1160, 1505
AflIII ACRYGT 1 cut(s) 230
AgeI ACCGGT 1 cut(s) 1197
AhlI ACTAGT 2 cut(s) 743, 1127
AjnI CCWGG 4 cut(s) 156, 169, 922, 1498
Alw21I GWGCWC 1 cut(s) 990
Alw26I GTCTC 4 cut(s) 323, 438, 506, 1429
AlwI GGATC 3 cut(s) 178, 1253, 1374
AlwNI CAGNNNCTG 1 cut(s) 1160
Ama87I CYCGRG 2 cut(s) 313, 1613
Aor13HI TCCGGA 1 cut(s) 1457
AoxI GGCC 6 cut(s) 45, 1106, 1151, 1203, 1507, 1565
ApaI GGGCCC 1 cut(s) 1110
ApeKI GCWGC 8 cut(s) 17, 29, 38, 365, 577, 580, 721, 1012
ApoI RAATTY 4 cut(s) 338, 1004, 1270, 1654
AsiGI ACCGGT 1 cut(s) 1197
Asp700I GAANNNNTTC 2 cut(s) 1241, 1325
AspLEI GCGC 4 cut(s) 7, 228, 1348, 1641
AspS9I GGNCC 6 cut(s) 240, 1055, 1106, 1107, 1507, 1618
AsuC2I CCSGG 2 cut(s) 1614, 1615
AsuHPI GGTGA 2 cut(s) 553, 1488
AvaI CYCGRG 2 cut(s) 313, 1613
AvaII GGWCC 3 cut(s) 240, 1055, 1618
BaeGI GKGCMC 2 cut(s) 1110, 1390
BalI TGGCCA 1 cut(s) 1205
BanII GRGCYC 3 cut(s) 990, 1030, 1110
BbsI GAAGAC 1 cut(s) 391
Bbv12I GWGCWC 1 cut(s) 990
BbvCI CCTCAGC 1 cut(s) 42
BbvI GCAGC 8 cut(s) 16, 25, 29, 377, 589, 592, 733, 999
BccI CCATC 9 cut(s) 212, 433, 481, 541, 757, 999, 1214, 1484, 1639
BceAI ACGGC 1 cut(s) 685
BcgI CGANNNNNNTGC 2 cut(s) 456, 490
BciT130I CCWGG 4 cut(s) 158, 171, 924, 1500
BciVI GTATCC 2 cut(s) 91, 1656
BclI TGATCA 1 cut(s) 876
BcnI CCSGG 2 cut(s) 1614, 1615
BcoDI GTCTC 4 cut(s) 323, 438, 506, 1429
BcuI ACTAGT 2 cut(s) 743, 1127
BfaI CTAG 4 cut(s) 744, 753, 1128, 1178
BfoI RGCGCY 2 cut(s) 8, 1642
BfuAI ACCTGC 2 cut(s) 496, 1387
BfuI GTATCC 2 cut(s) 91, 1656
BmcAI AGTACT 1 cut(s) 104
Bme1390I CCNGG 6 cut(s) 158, 171, 924, 1500, 1614, 1615
Bme18I GGWCC 3 cut(s) 240, 1055, 1618
BmeT110I CYCGRG 2 cut(s) 313, 1613
BmgT120I GGNCC 6 cut(s) 240, 1055, 1106, 1107, 1507, 1618
BmiI GGNNCC 5 cut(s) 486, 1029, 1107, 1108, 1619
BmrFI CCNGG 6 cut(s) 158, 171, 924, 1500, 1614, 1615
BmrI ACTGGG 1 cut(s) 1392
BmsI GCATC 5 cut(s) 65, 352, 643, 1078, 1486
BmuI ACTGGG 1 cut(s) 1392
BoxI GACNNNNGTC 1 cut(s) 150
BpiI GAAGAC 1 cut(s) 391
BpmI CTGGAG 1 cut(s) 1607
Bpu10I CCTNAGC 2 cut(s) 42, 1648
BpuEI CTTGAG 3 cut(s) 83, 958, 1453
BpuMI CCSGG 2 cut(s) 1614, 1615
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 1365
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 8 cut(s) 312, 922, 923, 1016, 1499, 1612, 1613, 1614
BsaWI WCCGGW 3 cut(s) 299, 1197, 1457
BsaXI ACNNNNNCTCC 2 cut(s) 710, 740
Bsc4I CCNNNNNNNGG 3 cut(s) 929, 1160, 1505
Bse118I RCCGGY 1 cut(s) 1197
Bse1I ACTGG 4 cut(s) 1154, 1165, 1387, 1624
Bse8I GATNNNNATC 1 cut(s) 1365
BseAI TCCGGA 1 cut(s) 1457
BseBI CCWGG 4 cut(s) 158, 171, 924, 1500
BseDI CCNNGG 8 cut(s) 312, 922, 923, 1016, 1499, 1612, 1613, 1614
BseGI GGATG 5 cut(s) 1206, 1366, 1477, 1495, 1631
BseJI GATNNNNATC 1 cut(s) 1365
BseLI CCNNNNNNNGG 3 cut(s) 929, 1160, 1505
BseMII CTCAG 4 cut(s) 33, 357, 449, 1662
BseNI ACTGG 4 cut(s) 1154, 1165, 1387, 1624
BseRI GAGGAG 1 cut(s) 1216
BseSI GKGCMC 2 cut(s) 1110, 1390
BseXI GCAGC 8 cut(s) 16, 25, 29, 377, 589, 592, 733, 999
Bsh1236I CGCG 2 cut(s) 50, 232
BshFI GGCC 6 cut(s) 47, 1108, 1153, 1205, 1509, 1567
BshTI ACCGGT 1 cut(s) 1197
BsiHKAI GWGCWC 1 cut(s) 990
BsiHKCI CYCGRG 2 cut(s) 313, 1613
BsiSI CCGG 4 cut(s) 300, 1198, 1458, 1614
BslFI GGGAC 3 cut(s) 128, 455, 905
BslI CCNNNNNNNGG 3 cut(s) 929, 1160, 1505
BsmAI GTCTC 4 cut(s) 323, 438, 506, 1429
BsmFI GGGAC 3 cut(s) 128, 455, 905
BsmI GAATGC 2 cut(s) 583, 792
BsnI GGCC 6 cut(s) 47, 1108, 1153, 1205, 1509, 1567
Bso31I GGTCTC 1 cut(s) 323
BsoBI CYCGRG 2 cut(s) 313, 1613
Bsp120I GGGCCC 1 cut(s) 1106
Bsp1286I GDGCHC 4 cut(s) 990, 1030, 1110, 1390
Bsp13I TCCGGA 1 cut(s) 1457
Bsp143I GATC 6 cut(s) 88, 183, 876, 1258, 1291, 1366
BspACI CCGC 6 cut(s) 48, 492, 724, 730, 1168, 1441
BspANI GGCC 6 cut(s) 47, 1108, 1153, 1205, 1509, 1567
BspCNI CTCAG 4 cut(s) 34, 356, 448, 1661
BspEI TCCGGA 1 cut(s) 1457
BspFNI CGCG 2 cut(s) 50, 232
BspLI GGNNCC 5 cut(s) 486, 1029, 1107, 1108, 1619
BspMI ACCTGC 2 cut(s) 496, 1387
BspPI GGATC 3 cut(s) 178, 1253, 1374
BspQI GCTCTTC 1 cut(s) 978
BspTNI GGTCTC 1 cut(s) 323
BsrFI RCCGGY 1 cut(s) 1197
BsrI ACTGG 4 cut(s) 1154, 1165, 1387, 1624
BssAI RCCGGY 1 cut(s) 1197
BssECI CCNNGG 8 cut(s) 312, 922, 923, 1016, 1499, 1612, 1613, 1614
BssMI GATC 6 cut(s) 88, 183, 876, 1258, 1291, 1366
BssT1I CCWWGG 1 cut(s) 1016
Bst2UI CCWGG 4 cut(s) 158, 171, 924, 1500
Bst4CI ACNGT 5 cut(s) 418, 666, 678, 1392, 1453
Bst6I CTCTTC 6 cut(s) 326, 600, 978, 1092, 1236, 1433
BstAPI GCANNNNNTGC 1 cut(s) 727
BstBAI YACGTR 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 6 cut(s) 42, 92, 343, 375, 435, 1648
BstEII GGTNACC 1 cut(s) 1494
BstF5I GGATG 5 cut(s) 1206, 1366, 1477, 1495, 1631
BstFNI CGCG 2 cut(s) 50, 232
BstH2I RGCGCY 2 cut(s) 8, 1642
BstHHI GCGC 4 cut(s) 7, 228, 1348, 1641
BstKTI GATC 6 cut(s) 91, 186, 879, 1261, 1294, 1369
BstMAI GTCTC 4 cut(s) 323, 438, 506, 1429
BstMBI GATC 6 cut(s) 88, 183, 876, 1258, 1291, 1366
BstNI CCWGG 4 cut(s) 158, 171, 924, 1500
BstNSI RCATGY 1 cut(s) 530
BstPAI GACNNNNGTC 1 cut(s) 150
BstPI GGTNACC 1 cut(s) 1494
BstSCI CCNGG 6 cut(s) 156, 169, 922, 1498, 1612, 1613
BstSLI GKGCMC 2 cut(s) 1110, 1390
BstUI CGCG 2 cut(s) 50, 232
BstV1I GCAGC 8 cut(s) 16, 25, 29, 377, 589, 592, 733, 999
BstV2I GAAGAC 1 cut(s) 391
BstX2I RGATCY 1 cut(s) 1366
BstXI CCANNNNNNTGG 2 cut(s) 480, 1023
BstYI RGATCY 1 cut(s) 1366
BsuI GTATCC 2 cut(s) 91, 1656
BsuRI GGCC 6 cut(s) 47, 1108, 1153, 1205, 1509, 1567
BtsCI GGATG 5 cut(s) 1206, 1366, 1477, 1495, 1631
BtsI GCAGTG 2 cut(s) 1028, 1341
BtsIMutI CAGTG 4 cut(s) 1028, 1045, 1161, 1341
BveI ACCTGC 2 cut(s) 496, 1387
Cac8I GCNNGC 1 cut(s) 230
CaiI CAGNNNCTG 1 cut(s) 1160
CfoI GCGC 4 cut(s) 7, 228, 1348, 1641
Cfr10I RCCGGY 1 cut(s) 1197
Cfr13I GGNCC 6 cut(s) 240, 1055, 1106, 1107, 1507, 1618
Cfr9I CCCGGG 1 cut(s) 1613
Csp6I GTAC 3 cut(s) 103, 443, 667
CspAI ACCGGT 1 cut(s) 1197
CviAII CATG 5 cut(s) 480, 527, 631, 863, 1334
CviQI GTAC 3 cut(s) 103, 443, 667
DdeI CTNAG 6 cut(s) 42, 92, 343, 375, 435, 1648
DpnI GATC 6 cut(s) 90, 185, 878, 1260, 1293, 1368
DpnII GATC 6 cut(s) 88, 183, 876, 1258, 1291, 1366
EaeI YGGCCR 1 cut(s) 1203
Eam1104I CTCTTC 6 cut(s) 326, 600, 978, 1092, 1236, 1433
EarI CTCTTC 6 cut(s) 326, 600, 978, 1092, 1236, 1433
Ecl136II GAGCTC 1 cut(s) 988
Eco130I CCWWGG 1 cut(s) 1016
Eco24I GRGCYC 3 cut(s) 990, 1030, 1110
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 3 cut(s) 240, 1055, 1618
Eco53kI GAGCTC 1 cut(s) 988
Eco57I CTGAAG 2 cut(s) 54, 1073
Eco88I CYCGRG 2 cut(s) 313, 1613
Eco91I GGTNACC 1 cut(s) 1494
EcoICRI GAGCTC 1 cut(s) 988
EcoO109I RGGNCCY 2 cut(s) 1106, 1618
EcoO65I GGTNACC 1 cut(s) 1494
EcoRI GAATTC 1 cut(s) 338
EcoRII CCWGG 4 cut(s) 156, 169, 922, 1498
EcoT14I CCWWGG 1 cut(s) 1016
EcoT38I GRGCYC 3 cut(s) 990, 1030, 1110
ErhI CCWWGG 1 cut(s) 1016
FaeI CATG 5 cut(s) 483, 530, 634, 866, 1337
FaqI GGGAC 3 cut(s) 128, 455, 905
FatI CATG 5 cut(s) 479, 526, 630, 862, 1333
FbaI TGATCA 1 cut(s) 876
FblI GTMKAC 1 cut(s) 1608
FokI GGATG 5 cut(s) 1193, 1373, 1464, 1502, 1618
FriOI GRGCYC 3 cut(s) 990, 1030, 1110
FspBI CTAG 4 cut(s) 744, 753, 1128, 1178
GlaI GCGC 4 cut(s) 6, 227, 1347, 1640
GsuI CTGGAG 1 cut(s) 1607
HaeII RGCGCY 2 cut(s) 8, 1642
HaeIII GGCC 6 cut(s) 47, 1108, 1153, 1205, 1509, 1567
HapII CCGG 4 cut(s) 300, 1198, 1458, 1614
HhaI GCGC 4 cut(s) 7, 228, 1348, 1641
Hin1II CATG 5 cut(s) 483, 530, 634, 866, 1337
Hin6I GCGC 4 cut(s) 5, 226, 1346, 1639
HinP1I GCGC 4 cut(s) 5, 226, 1346, 1639
HincII GTYRAC 1 cut(s) 1591
HindII GTYRAC 1 cut(s) 1591
HindIII AAGCTT 2 cut(s) 687, 1112
HinfI GANTC 7 cut(s) 116, 918, 933, 1283, 1355, 1437, 1521
HpaII CCGG 4 cut(s) 300, 1198, 1458, 1614
HphI GGTGA 2 cut(s) 553, 1488
Hpy166II GTNNAC 3 cut(s) 1058, 1591, 1609
Hpy188I TCNGA 6 cut(s) 244, 271, 438, 682, 1236, 1360
Hpy188III TCNNGA 9 cut(s) 120, 425, 937, 1178, 1432, 1458, 1481, 1518, 1650
Hpy8I GTNNAC 3 cut(s) 1058, 1591, 1609
Hpy99I CGWCG 4 cut(s) 143, 146, 149, 395
HpyAV CCTTC 3 cut(s) 619, 1055, 1175
HpyCH4III ACNGT 5 cut(s) 418, 666, 678, 1392, 1453
HpyCH4IV ACGT 2 cut(s) 192, 448
HpyCH4V TGCA 7 cut(s) 17, 56, 108, 365, 778, 1341, 1396
HpyF3I CTNAG 6 cut(s) 42, 92, 343, 375, 435, 1648
HpySE526I ACGT 2 cut(s) 192, 448
Hsp92II CATG 5 cut(s) 483, 530, 634, 866, 1337
HspAI GCGC 4 cut(s) 5, 226, 1346, 1639
Kpn2I TCCGGA 1 cut(s) 1457
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 6 cut(s) 88, 183, 876, 1258, 1291, 1366
LguI GCTCTTC 1 cut(s) 978
LmnI GCTCC 5 cut(s) 25, 718, 993, 1033, 1460
Lsp1109I GCAGC 8 cut(s) 16, 25, 29, 377, 589, 592, 733, 999
LweI GCATC 5 cut(s) 65, 352, 643, 1078, 1486
MaeI CTAG 4 cut(s) 744, 753, 1128, 1178
MaeII ACGT 2 cut(s) 192, 448
MaeIII GTNAC 5 cut(s) 846, 1040, 1156, 1378, 1494
MalI GATC 6 cut(s) 90, 185, 878, 1260, 1293, 1368
MboI GATC 6 cut(s) 88, 183, 876, 1258, 1291, 1366
MfeI CAATTG 3 cut(s) 60, 978, 1467
MflI RGATCY 1 cut(s) 1366
MhlI GDGCHC 4 cut(s) 990, 1030, 1110, 1390
MlsI TGGCCA 1 cut(s) 1205
MluI ACGCGT 1 cut(s) 230
MluNI TGGCCA 1 cut(s) 1205
MlyI GAGTC 5 cut(s) 110, 927, 1364, 1431, 1515
MmeI TCCRAC 3 cut(s) 283, 1270, 1338
Mox20I TGGCCA 1 cut(s) 1205
MroI TCCGGA 1 cut(s) 1457
MroXI GAANNNNTTC 2 cut(s) 1241, 1325
MscI TGGCCA 1 cut(s) 1205
MseI TTAA 4 cut(s) 459, 822, 962, 1100
MslI CAYNNNNRTG 2 cut(s) 478, 569
Msp20I TGGCCA 1 cut(s) 1205
MspA1I CMGCKG 3 cut(s) 605, 724, 1012
MspI CCGG 4 cut(s) 300, 1198, 1458, 1614
MspR9I CCNGG 6 cut(s) 158, 171, 924, 1500, 1614, 1615
MunI CAATTG 3 cut(s) 60, 978, 1467
Mva1269I GAATGC 2 cut(s) 583, 792
MvaI CCWGG 4 cut(s) 158, 171, 924, 1500
MvnI CGCG 2 cut(s) 50, 232
NciI CCSGG 2 cut(s) 1614, 1615
NdeII GATC 6 cut(s) 88, 183, 876, 1258, 1291, 1366
NlaIII CATG 5 cut(s) 483, 530, 634, 866, 1337
NlaIV GGNNCC 5 cut(s) 486, 1029, 1107, 1108, 1619
NmeAIII GCCGAG 1 cut(s) 1580
NmuCI GTSAC 3 cut(s) 1040, 1156, 1494
NspI RCATGY 1 cut(s) 530
PasI CCCWGGG 1 cut(s) 923
PciSI GCTCTTC 1 cut(s) 978
PctI GAATGC 2 cut(s) 583, 792
PdmI GAANNNNTTC 2 cut(s) 1241, 1325
PfeI GAWTC 2 cut(s) 933, 1283
PflFI GACNNNGTC 1 cut(s) 395
PflMI CCANNNNNTGG 2 cut(s) 929, 1160
PinAI ACCGGT 1 cut(s) 1197
PleI GAGTC 5 cut(s) 110, 926, 1363, 1431, 1515
PpsI GAGTC 5 cut(s) 110, 926, 1363, 1431, 1515
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 1 cut(s) 1618
PshAI GACNNNNGTC 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 990
Psp1406I AACGTT 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 1618
Psp6I CCWGG 4 cut(s) 156, 169, 922, 1498
PspEI GGTNACC 1 cut(s) 1494
PspGI CCWGG 4 cut(s) 156, 169, 922, 1498
PspN4I GGNNCC 5 cut(s) 486, 1029, 1107, 1108, 1619
PspOMI GGGCCC 1 cut(s) 1106
PspPI GGNCC 6 cut(s) 240, 1055, 1106, 1107, 1507, 1618
PspPPI RGGWCCY 1 cut(s) 1618
PstNI CAGNNNCTG 1 cut(s) 1160
PsuI RGATCY 1 cut(s) 1366
PsyI GACNNNGTC 1 cut(s) 395
PvuII CAGCTG 2 cut(s) 605, 1012
RsaI GTAC 3 cut(s) 104, 444, 668
RsaNI GTAC 3 cut(s) 103, 443, 667
RseI CAYNNNNRTG 2 cut(s) 478, 569
SacI GAGCTC 1 cut(s) 990
SapI GCTCTTC 1 cut(s) 978
SaqAI TTAA 4 cut(s) 459, 822, 962, 1100
Sau3AI GATC 6 cut(s) 88, 183, 876, 1258, 1291, 1366
Sau96I GGNCC 6 cut(s) 240, 1055, 1106, 1107, 1507, 1618
ScaI AGTACT 1 cut(s) 104
SchI GAGTC 5 cut(s) 110, 927, 1364, 1431, 1515
ScrFI CCNGG 6 cut(s) 158, 171, 924, 1500, 1614, 1615
SduI GDGCHC 4 cut(s) 990, 1030, 1110, 1390
SfaNI GCATC 5 cut(s) 65, 352, 643, 1078, 1486
SinI GGWCC 3 cut(s) 240, 1055, 1618
SmaI CCCGGG 1 cut(s) 1615
SmiMI CAYNNNNRTG 2 cut(s) 478, 569
SmlI CTYRAG 4 cut(s) 8, 98, 937, 1432
SmoI CTYRAG 4 cut(s) 8, 98, 937, 1432
SpeI ACTAGT 2 cut(s) 743, 1127
SsiI CCGC 6 cut(s) 48, 492, 724, 730, 1168, 1441
SspI AATATT 1 cut(s) 1423
SspMI CTAG 4 cut(s) 744, 753, 1128, 1178
SstI GAGCTC 1 cut(s) 990
StyD4I CCNGG 6 cut(s) 156, 169, 922, 1498, 1612, 1613
StyI CCWWGG 1 cut(s) 1016
TaaI ACNGT 5 cut(s) 418, 666, 678, 1392, 1453
TaiI ACGT 2 cut(s) 195, 451
TaqI TCGA 4 cut(s) 121, 390, 426, 1251
TatI WGTACW 1 cut(s) 102
TauI GCSGC 3 cut(s) 50, 494, 1171
TfiI GAWTC 2 cut(s) 933, 1283
Tru1I TTAA 4 cut(s) 459, 822, 962, 1100
Tru9I TTAA 4 cut(s) 459, 822, 962, 1100
TscAI CASTG 4 cut(s) 1028, 1045, 1161, 1348
TseFI GTSAC 3 cut(s) 1040, 1156, 1494
TseI GCWGC 8 cut(s) 17, 29, 38, 365, 577, 580, 721, 1012
Tsp45I GTSAC 3 cut(s) 1040, 1156, 1494
TspDTI ATGAA 4 cut(s) 391, 587, 855, 879
TspGWI ACGGA 1 cut(s) 1265
TspMI CCCGGG 1 cut(s) 1613
TspRI CASTG 4 cut(s) 1028, 1045, 1161, 1348
Tth111I GACNNNGTC 1 cut(s) 395
Van91I CCANNNNNTGG 2 cut(s) 929, 1160
VpaK11BI GGWCC 3 cut(s) 240, 1055, 1618
XapI RAATTY 4 cut(s) 338, 1004, 1270, 1654
XbaI TCTAGA 1 cut(s) 1177
XceI RCATGY 1 cut(s) 530
XmaI CCCGGG 1 cut(s) 1613
XmiI GTMKAC 1 cut(s) 1608
XmnI GAANNNNTTC 2 cut(s) 1241, 1325
XspI CTAG 4 cut(s) 744, 753, 1128, 1178
ZrmI AGTACT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.