AT4G25080

Magnesium-protoporphyrin IX methyltransferase C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009399
Physical Location & Seq
Forward (+)
12876822 .. 12878703
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25080.4

Sequence Viewer

Length: 939 bp
ATGCCGTTTGCTCCTTCCTTGTTGTCATCGTCTTCCTCAGTCTCTCAATTTCTTCCCAGATTCCCCAACGCGACTAGATTCAATGTAACTCCACGGAGCAGAGCCGCCACCGTCGTCGCGGCATCCGTCACCGACCTAGCCGGGGTCGACAGTACAACAATCGCCGTACTCGGTGGAGGATCCGTAGCAGCACTCGCGGCGATGGTTTCCTTAACGGATCCGGAGAGGAGGCGGAAATTGCAGGCGGAGGAAGTTGGCGGAGGCGATAAGGAGGTTGTGAGGGAGTATTTCAATAGCACGGGGTTCGAGAGGTGGAGGAAGATCTACGGTGAGACTGATGAAGTGAATCGAGTACAGAAGGATATTCGACTCGGTCATGCTAAGACGGTGGAGAACACGATGCTTATGCTGACTGAAGATAGATCCTTGGCCGGTGTCACGGTTTGCGACGCCGGTTGTGGAACCGGTTTGCTCTCGATTCCACTTGCTAAGGAAGGAGCAATCGTCTCTGCTTCCGATATTTCTGCTGCTATGGTTGCTGAAGCTGAGATGAAGGCAAAGGCACAACTACCATCAGAGAATTTACCAAAATTTGAGGTGAATGATTTGGAGAGCCTAACTGGGAAGTATGATACCGTTGTATGTCTCGACGTGTTGATACATTACCCGCAGAACAAAGCAGACGGAATGATCGCACATCTTGCTTCTTTAGCAGAGAAGAGAGTGATTCTGAGTTTTGCTCCAAAGACTTTTTACTATGATATCTTAAAGAGAATTGGAGAGCTTTTCCCAGGGCCATCAAAAGCTACAAGGGCGTATCTACACTCGGAGGCGGATGTGGAAAGAGCGTTGGGTAAAGTCGGCTGGAAAATCAGCAAGAGAGGACTCACTACCACACAGTTCTACTTCTCTAGGCTCATCGAAGCTGTTCCAATGTAG

Protein Analysis

312

Amino Acids

33.8

Weight (kDa)

7.68

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 77 - 224 1.2e-09 Methyltransferase domain
Methyltransf_31 PF13847 77 - 157 3.3e-09 Methyltransferase domain
Methyltransf_25 PF13649 81 - 170 6.5e-13 Methyltransferase domain
Methyltransf_12 PF08242 83 - 170 1.3e-10 Methyltransferase domain
Methyltransf_11 PF08241 83 - 156 4.3e-09 Methyltransferase domain
Mg-por_mtran_C PF07109 148 - 243 1.6e-37 Magnesium-protoporphyrin IX methyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009399)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25080 AT4G25080 AT4G25080 AT4G25080 AT4G25080 AT4G25080
fragaria_vesca FvH4_5g09430 FvH4_5g09430
malus_domestica MD06G1131500.v1.1 MD14G1147900.v1.1
prunus_persica Prupe.5G142500_v2.0.a1
pyrus_communis pycom06g12290
rosa_chinensis RchiOBHm_Chr7g0188761
rosa_laevigata RLG00000004663
rosa_multiflora Rmu_sc0042908.1_g000002
rosa_roxburghii Rroxscaffold_3G00266060
rosa_rugosa Rorug06G0493700
rosa_samantha Rh7AG098100 Rh7BG099800 Rh7CG101500 Rh7DG099900
rosa_wichuraiana Rw7G008470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 147
AccII CGCG 3 cut(s) 71, 119, 197
AccIII TCCGGA 1 cut(s) 220
AciI CCGC 8 cut(s) 105, 119, 197, 232, 245, 258, 668, 833
AclWI GGATC 5 cut(s) 174, 187, 212, 225, 417
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 2 cut(s) 580, 590
AcuI CTGAAG 2 cut(s) 435, 561
AcyI GRCGYC 1 cut(s) 450
AfaI GTAC 3 cut(s) 154, 168, 354
AfiI CCNNNNNNNGG 1 cut(s) 142
AflIII ACRYGT 1 cut(s) 651
AgeI ACCGGT 1 cut(s) 464
AgsI TTSAA 2 cut(s) 82, 292
AjiI CACGTC 1 cut(s) 652
AjnI CCWGG 1 cut(s) 790
AluBI AGCT 4 cut(s) 545, 784, 806, 926
AluI AGCT 4 cut(s) 545, 784, 806, 926
Alw26I GTCTC 4 cut(s) 46, 326, 511, 650
AlwI GGATC 5 cut(s) 174, 187, 212, 225, 417
Aor13HI TCCGGA 1 cut(s) 220
AoxI GGCC 2 cut(s) 429, 794
ApeKI GCWGC 2 cut(s) 188, 527
ApoI RAATTY 2 cut(s) 580, 590
AsiGI ACCGGT 1 cut(s) 464
Asp700I GAANNNNTTC 1 cut(s) 927
AspS9I GGNCC 1 cut(s) 794
AsuC2I CCSGG 1 cut(s) 142
AsuHPI GGTGA 3 cut(s) 121, 341, 610
BaeI ACNNNNGTAYC 2 cut(s) 624, 657
BamHI GGATCC 2 cut(s) 179, 217
BbsI GAAGAC 1 cut(s) 24
BbvI GCAGC 2 cut(s) 200, 514
BccI CCATC 3 cut(s) 196, 580, 805
BceAI ACGGC 1 cut(s) 149
BcgI CGANNNNNNTGC 6 cut(s) 286, 320, 388, 422, 506, 540
BciT130I CCWGG 1 cut(s) 792
BcnI CCSGG 1 cut(s) 142
BcoDI GTCTC 4 cut(s) 46, 326, 511, 650
BfaI CTAG 3 cut(s) 75, 137, 912
BglII AGATCT 1 cut(s) 321
BisI GCNGC 5 cut(s) 105, 120, 189, 198, 528
BlsI GCNGC 5 cut(s) 106, 121, 190, 199, 529
Bme1390I CCNGG 2 cut(s) 142, 792
BmgBI CACGTC 1 cut(s) 652
BmgT120I GGNCC 1 cut(s) 794
BmiI GGNNCC 3 cut(s) 181, 219, 463
BmrFI CCNGG 2 cut(s) 142, 792
BmrI ACTGGG 1 cut(s) 630
BmsI GCATC 2 cut(s) 131, 390
BmuI ACTGGG 1 cut(s) 630
BpiI GAAGAC 1 cut(s) 24
BplI GAGNNNNNCTC 2 cut(s) 724, 756
Bpu10I CCTNAGC 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 142
BsaHI GRCGYC 1 cut(s) 450
BsaJI CCNNGG 5 cut(s) 92, 141, 426, 790, 791
BsaWI WCCGGW 2 cut(s) 220, 464
BsaXI ACNNNNNCTCC 4 cut(s) 168, 198, 489, 519
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse118I RCCGGY 3 cut(s) 431, 452, 464
Bse1I ACTGG 1 cut(s) 625
BseAI TCCGGA 1 cut(s) 220
BseBI CCWGG 1 cut(s) 792
BseDI CCNNGG 5 cut(s) 92, 141, 426, 790, 791
BseGI GGATG 2 cut(s) 122, 841
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 3 cut(s) 51, 537, 722
BseNI ACTGG 1 cut(s) 625
BseRI GAGGAG 1 cut(s) 241
BseXI GCAGC 2 cut(s) 200, 514
Bsh1236I CGCG 3 cut(s) 71, 119, 197
BshFI GGCC 2 cut(s) 431, 796
BshTI ACCGGT 1 cut(s) 464
BsiSI CCGG 5 cut(s) 141, 221, 432, 453, 465
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 4 cut(s) 46, 326, 511, 650
BsmBI CGTCTC 1 cut(s) 511
BsnI GGCC 2 cut(s) 431, 796
Bsp13I TCCGGA 1 cut(s) 220
Bsp143I GATC 5 cut(s) 179, 217, 321, 422, 690
BspACI CCGC 8 cut(s) 105, 119, 197, 232, 245, 258, 668, 833
BspANI GGCC 2 cut(s) 431, 796
BspCNI CTCAG 3 cut(s) 50, 538, 723
BspEI TCCGGA 1 cut(s) 220
BspFNI CGCG 3 cut(s) 71, 119, 197
BspLI GGNNCC 3 cut(s) 181, 219, 463
BspPI GGATC 5 cut(s) 174, 187, 212, 225, 417
BsrFI RCCGGY 3 cut(s) 431, 452, 464
BsrI ACTGG 1 cut(s) 625
BssAI RCCGGY 3 cut(s) 431, 452, 464
BssECI CCNNGG 5 cut(s) 92, 141, 426, 790, 791
BssMI GATC 5 cut(s) 179, 217, 321, 422, 690
BssNI GRCGYC 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 792
Bst4CI ACNGT 7 cut(s) 112, 152, 329, 388, 442, 637, 900
Bst6I CTCTTC 1 cut(s) 713
BstACI GRCGYC 1 cut(s) 450
BstAPI GCANNNNNTGC 1 cut(s) 701
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 5 cut(s) 37, 381, 489, 546, 731
BstDSI CCRYGG 1 cut(s) 92
BstF5I GGATG 2 cut(s) 122, 841
BstFNI CGCG 3 cut(s) 71, 119, 197
BstKTI GATC 5 cut(s) 182, 220, 324, 425, 693
BstMAI GTCTC 4 cut(s) 46, 326, 511, 650
BstMBI GATC 5 cut(s) 179, 217, 321, 422, 690
BstMWI GCNNNNNNNGC 7 cut(s) 194, 197, 238, 536, 701, 710, 812
BstNI CCWGG 1 cut(s) 792
BstSCI CCNGG 2 cut(s) 140, 790
BstUI CGCG 3 cut(s) 71, 119, 197
BstV1I GCAGC 2 cut(s) 200, 514
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 4 cut(s) 179, 217, 321, 422
BstYI RGATCY 4 cut(s) 179, 217, 321, 422
BsuRI GGCC 2 cut(s) 431, 796
BtgI CCRYGG 1 cut(s) 92
BtgZI GCGATG 1 cut(s) 215
BtrI CACGTC 1 cut(s) 652
BtsCI GGATG 2 cut(s) 122, 841
Cac8I GCNNGC 1 cut(s) 243
Cfr10I RCCGGY 3 cut(s) 431, 452, 464
Cfr13I GGNCC 1 cut(s) 794
CseI GACGC 1 cut(s) 458
Csp6I GTAC 3 cut(s) 153, 167, 353
CspAI ACCGGT 1 cut(s) 464
CviAII CATG 1 cut(s) 377
CviQI GTAC 3 cut(s) 153, 167, 353
DdeI CTNAG 5 cut(s) 37, 381, 489, 546, 731
DpnI GATC 5 cut(s) 181, 219, 323, 424, 692
DpnII GATC 5 cut(s) 179, 217, 321, 422, 690
EaeI YGGCCR 1 cut(s) 429
Eam1104I CTCTTC 1 cut(s) 713
EarI CTCTTC 1 cut(s) 713
EciI GGCGGA 4 cut(s) 247, 260, 273, 848
Eco130I CCWWGG 1 cut(s) 426
Eco32I GATATC 1 cut(s) 763
Eco57I CTGAAG 2 cut(s) 435, 561
EcoRII CCWGG 1 cut(s) 790
EcoRV GATATC 1 cut(s) 763
EcoT14I CCWWGG 1 cut(s) 426
ErhI CCWWGG 1 cut(s) 426
Esp3I CGTCTC 1 cut(s) 511
FaeI CATG 1 cut(s) 380
FaiI YATR 6 cut(s) 378, 407, 533, 630, 643, 759
FatI CATG 1 cut(s) 376
FauI CCCGC 1 cut(s) 675
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 5 cut(s) 105, 120, 189, 198, 528
FokI GGATG 2 cut(s) 109, 848
Fsp4HI GCNGC 5 cut(s) 105, 120, 189, 198, 528
FspBI CTAG 3 cut(s) 75, 137, 912
GluI GCNGC 5 cut(s) 105, 120, 189, 198, 528
HaeIII GGCC 2 cut(s) 431, 796
HapII CCGG 5 cut(s) 141, 221, 432, 453, 465
HgaI GACGC 1 cut(s) 458
Hin1I GRCGYC 1 cut(s) 450
Hin1II CATG 1 cut(s) 380
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 7 cut(s) 60, 78, 346, 369, 478, 727, 885
HpaII CCGG 5 cut(s) 141, 221, 432, 453, 465
HphI GGTGA 3 cut(s) 121, 341, 610
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 4 cut(s) 517, 577, 732, 829
Hpy188III TCNNGA 4 cut(s) 221, 307, 475, 647
Hpy8I GTNNAC 1 cut(s) 148
Hpy99I CGWCG 4 cut(s) 116, 119, 452, 653
HpyAV CCTTC 4 cut(s) 24, 352, 488, 547
HpyCH4III ACNGT 7 cut(s) 112, 152, 329, 388, 442, 637, 900
HpyCH4IV ACGT 1 cut(s) 651
HpyCH4V TGCA 1 cut(s) 241
HpyF10VI GCNNNNNNNGC 7 cut(s) 194, 197, 238, 536, 701, 710, 812
HpyF3I CTNAG 5 cut(s) 37, 381, 489, 546, 731
HpySE526I ACGT 1 cut(s) 651
Hsp92I GRCGYC 1 cut(s) 450
Hsp92II CATG 1 cut(s) 380
Kpn2I TCCGGA 1 cut(s) 220
Kzo9I GATC 5 cut(s) 179, 217, 321, 422, 690
LmnI GCTCC 4 cut(s) 16, 96, 497, 745
Lsp1109I GCAGC 2 cut(s) 200, 514
LweI GCATC 2 cut(s) 131, 390
MaeI CTAG 3 cut(s) 75, 137, 912
MaeII ACGT 1 cut(s) 651
MaeIII GTNAC 3 cut(s) 85, 127, 436
MalI GATC 5 cut(s) 181, 219, 323, 424, 692
MboI GATC 5 cut(s) 179, 217, 321, 422, 690
MboII GAAGA 5 cut(s) 24, 44, 331, 428, 730
MflI RGATCY 4 cut(s) 179, 217, 321, 422
MluCI AATT 5 cut(s) 47, 236, 580, 590, 774
MlyI GAGTC 2 cut(s) 363, 879
MroI TCCGGA 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 927
MseI TTAA 2 cut(s) 212, 767
MspI CCGG 5 cut(s) 141, 221, 432, 453, 465
MspR9I CCNGG 2 cut(s) 142, 792
MvaI CCWGG 1 cut(s) 792
MvnI CGCG 3 cut(s) 71, 119, 197
MwoI GCNNNNNNNGC 7 cut(s) 194, 197, 238, 536, 701, 710, 812
NciI CCSGG 1 cut(s) 142
NdeII GATC 5 cut(s) 179, 217, 321, 422, 690
NlaIII CATG 1 cut(s) 380
NlaIV GGNNCC 3 cut(s) 181, 219, 463
NmuCI GTSAC 2 cut(s) 127, 436
PasI CCCWGGG 1 cut(s) 791
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 927
PfeI GAWTC 5 cut(s) 60, 78, 346, 478, 727
PflFI GACNNNGTC 1 cut(s) 372
PinAI ACCGGT 1 cut(s) 464
PkrI GCNGC 5 cut(s) 106, 121, 190, 199, 529
PleI GAGTC 2 cut(s) 363, 879
PpsI GAGTC 2 cut(s) 363, 879
Psp6I CCWGG 1 cut(s) 790
PspGI CCWGG 1 cut(s) 790
PspN4I GGNNCC 3 cut(s) 181, 219, 463
PspPI GGNCC 1 cut(s) 794
PsuI RGATCY 4 cut(s) 179, 217, 321, 422
PsyI GACNNNGTC 1 cut(s) 372
RsaI GTAC 3 cut(s) 154, 168, 354
RsaNI GTAC 3 cut(s) 153, 167, 353
SalI GTCGAC 1 cut(s) 146
SaqAI TTAA 2 cut(s) 212, 767
SatI GCNGC 5 cut(s) 105, 120, 189, 198, 528
Sau3AI GATC 5 cut(s) 179, 217, 321, 422, 690
Sau96I GGNCC 1 cut(s) 794
SchI GAGTC 2 cut(s) 363, 879
ScrFI CCNGG 2 cut(s) 142, 792
SetI ASST 9 cut(s) 138, 276, 314, 547, 600, 654, 786, 808, 928
SfaNI GCATC 2 cut(s) 131, 390
Sse9I AATT 5 cut(s) 47, 236, 580, 590, 774
SsiI CCGC 8 cut(s) 105, 119, 197, 232, 245, 258, 668, 833
SspMI CTAG 3 cut(s) 75, 137, 912
StyD4I CCNGG 2 cut(s) 140, 790
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 7 cut(s) 112, 152, 329, 388, 442, 637, 900
TaiI ACGT 1 cut(s) 654
TaqI TCGA 7 cut(s) 147, 306, 349, 367, 476, 648, 921
TaqII GACCGA 1 cut(s) 362
TasI AATT 5 cut(s) 47, 236, 580, 590, 774
TatI WGTACW 2 cut(s) 152, 352
TauI GCSGC 3 cut(s) 107, 122, 200
TfiI GAWTC 5 cut(s) 60, 78, 346, 478, 727
Tru1I TTAA 2 cut(s) 212, 767
Tru9I TTAA 2 cut(s) 212, 767
TseFI GTSAC 2 cut(s) 127, 436
TseI GCWGC 2 cut(s) 188, 527
Tsp45I GTSAC 2 cut(s) 127, 436
TspDTI ATGAA 2 cut(s) 354, 566
TspGWI ACGGA 5 cut(s) 109, 115, 172, 230, 699
Tth111I GACNNNGTC 1 cut(s) 372
XapI RAATTY 2 cut(s) 580, 590
XmiI GTMKAC 1 cut(s) 147
XmnI GAANNNNTTC 1 cut(s) 927
XspI CTAG 3 cut(s) 75, 137, 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.