Rroxscaffold_3G00266060

Magnesium protoporphyrin IX methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009399
Physical Location & Seq
Forward (+)
59446417 .. 59449952
3536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00266060.1

Sequence Viewer

Length: 948 bp
ATGGCATTCTCCGCCTCTCTCTCTTCCCCACTCCACTTCCGCAACCCGAACCCTATATTCTCTCCCAAGCCCACTAAACCCGCAACAAGACCCGCCACCCTCAAGCCCCTCCTCGCCCTCCCGCCCTCCGTCGCCGCCGACGTCGACAGCGCCACCATCGCCGCTATCGGCGGCGGCTCCGTGGCCGCACTCGCCGCCGCGCTCTCCCTCGCCGACCCCGAGCGCCGGCGGAAGCTCCAGGCCGAGGAGGTCGGCGGCGGCGACAAGGAGGTCGTCAAGGACTACTTCAACAAGTCGGGGTTCGAGCGGTGGAAGAAGATTTACGGCGAGACGGATGACGTCAACACAGTTCAGAGGGATATCAGGCTCGGCCATTCGAAGACCGTCGAGAATGTGATGAAGATGTTGACGGATGAAGGTCCGCTTGGTGGCGTCACCGTCTGTGACGCCGGCTGCGGCACCGGCTGTCTCGCTATCCCGCTCGCTAATGAAGGCGCCCTCGTCACCGCCACCGACATTTCCGCCGCCATGGTCGCCGAGGCAGAGAAGCAGGCGAGAGAGCAGCTTGATTTGGGCAAAGAGGGAGTCGTATTGCCGAAATTCGAAGTGAGTGATTTAGAGAGCTTGGAAGGGAAGTATGACACGGTGGTGTGTCTGGATGTGTTGATTCATTACCCACAGAGCAAGGCGGATGGGATGATTGCCCACCTTGCATCACTGGCCGAGAATCGGTTGCTTCTGAGCTTTGCACCCAAGACATTGTATTACGATATCCTGAAGAGGATAGGAGAGTTCTTCCCCGGGCCTTCAAAGGCGACGAGGGCGTATCTGCACTCTGAGGTGGATGTGGAGAGGGCTTTGAAGAAGGTTGGGTGGAGGATAAGGAAGAGAGGACTAACAACAACACAGTTCTATTTTTCGAGGCTTGTTGAGGCTGTTCCTATTTAA

Protein Analysis

315

Amino Acids

34.03

Weight (kDa)

6.97

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 144 - 227 2.9e-09 Methyltransferase domain
Methyltransf_23 PF13489 146 - 295 2.8e-10 Methyltransferase domain
Methyltransf_25 PF13649 147 - 239 6.3e-13 Methyltransferase domain
Methyltransf_12 PF08242 149 - 240 8.1e-12 Methyltransferase domain
Methyltransf_11 PF08241 149 - 226 7.6e-10 Methyltransferase domain
Mg-por_mtran_C PF07109 218 - 313 2.6e-36 Magnesium-protoporphyrin IX methyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009399)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25080 AT4G25080 AT4G25080 AT4G25080 AT4G25080 AT4G25080
fragaria_vesca FvH4_5g09430 FvH4_5g09430
malus_domestica MD06G1131500.v1.1 MD14G1147900.v1.1
prunus_persica Prupe.5G142500_v2.0.a1
pyrus_communis pycom06g12290
rosa_chinensis RchiOBHm_Chr7g0188761
rosa_laevigata RLG00000004663
rosa_multiflora Rmu_sc0042908.1_g000002
rosa_roxburghii Rroxscaffold_3G00266060
rosa_rugosa Rorug06G0493700
rosa_samantha Rh7AG098100 Rh7BG099800 Rh7CG101500 Rh7DG099900
rosa_wichuraiana Rw7G008470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
AatII GACGTC 2 cut(s) 144, 342
AccB1I GGYRCC 2 cut(s) 458, 494
AccBSI CCGCTC 2 cut(s) 307, 481
AccI GTMKAC 1 cut(s) 144
AccII CGCG 1 cut(s) 200
AcoI YGGCCR 3 cut(s) 183, 370, 720
AcsI RAATTY 1 cut(s) 599
AcuI CTGAAG 1 cut(s) 797
AcyI GRCGYC 5 cut(s) 141, 339, 432, 447, 495
AfiI CCNNNNNNNGG 4 cut(s) 225, 244, 428, 729
AgsI TTSAA 3 cut(s) 289, 810, 862
AjnI CCWGG 1 cut(s) 237
AjuI GAANNNNNNNTTGG 2 cut(s) 408, 440
AleI CACNNNNGTG 1 cut(s) 647
AluBI AGCT 4 cut(s) 235, 565, 624, 744
AluI AGCT 4 cut(s) 235, 565, 624, 744
Alw26I GTCTC 2 cut(s) 323, 473
Ama87I CYCGRG 2 cut(s) 218, 800
AoxI GGCC 5 cut(s) 183, 240, 370, 720, 803
ApeKI GCWGC 2 cut(s) 453, 562
ApoI RAATTY 1 cut(s) 599
ArsI GACNNNNNNTTYG 2 cut(s) 570, 602
AspLEI GCGC 4 cut(s) 152, 202, 225, 497
AspS9I GGNCC 2 cut(s) 419, 803
AsuC2I CCSGG 2 cut(s) 801, 802
AsuHPI GGTGA 2 cut(s) 427, 496
AsuII TTCGAA 2 cut(s) 377, 603
AvaI CYCGRG 2 cut(s) 218, 800
AvaII GGWCC 1 cut(s) 419
BanI GGYRCC 2 cut(s) 458, 494
BarI GAAGNNNNNNTAC 2 cut(s) 305, 337
BbsI GAAGAC 1 cut(s) 386
BbvI GCAGC 2 cut(s) 440, 574
BccI CCATC 2 cut(s) 164, 686
BceAI ACGGC 1 cut(s) 340
BciT130I CCWGG 1 cut(s) 239
BcnI CCSGG 2 cut(s) 801, 802
BcoDI GTCTC 2 cut(s) 323, 473
BfoI RGCGCY 3 cut(s) 153, 226, 498
Bme1390I CCNGG 3 cut(s) 239, 801, 802
Bme18I GGWCC 1 cut(s) 419
BmeT110I CYCGRG 2 cut(s) 218, 800
BmgT120I GGNCC 2 cut(s) 419, 803
BmiI GGNNCC 3 cut(s) 178, 460, 496
BmrFI CCNGG 3 cut(s) 239, 801, 802
BmsI GCATC 1 cut(s) 722
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 1 cut(s) 221
Bpu14I TTCGAA 2 cut(s) 377, 603
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 2 cut(s) 801, 802
BsaHI GRCGYC 5 cut(s) 141, 339, 432, 447, 495
BsaJI CCNNGG 6 cut(s) 180, 243, 528, 537, 799, 800
Bsc4I CCNNNNNNNGG 4 cut(s) 225, 244, 428, 729
Bse118I RCCGGY 3 cut(s) 225, 449, 461
Bse1I ACTGG 1 cut(s) 723
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 6 cut(s) 180, 243, 528, 537, 799, 800
BseGI GGATG 6 cut(s) 340, 418, 664, 697, 702, 850
BseLI CCNNNNNNNGG 4 cut(s) 225, 244, 428, 729
BseMII CTCAG 2 cut(s) 731, 828
BseNI ACTGG 1 cut(s) 723
BseRI GAGGAG 2 cut(s) 101, 260
BseXI GCAGC 2 cut(s) 440, 574
BsgI GTGCAG 1 cut(s) 815
Bsh1236I CGCG 1 cut(s) 200
BshFI GGCC 5 cut(s) 185, 242, 372, 722, 805
BshNI GGYRCC 2 cut(s) 458, 494
BsiHKCI CYCGRG 2 cut(s) 218, 800
BsiSI CCGG 4 cut(s) 226, 450, 462, 801
BslI CCNNNNNNNGG 4 cut(s) 225, 244, 428, 729
BsmAI GTCTC 2 cut(s) 323, 473
BsmBI CGTCTC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 5 cut(s) 185, 242, 372, 722, 805
BsoBI CYCGRG 2 cut(s) 218, 800
Bsp119I TTCGAA 2 cut(s) 377, 603
Bsp19I CCATGG 1 cut(s) 528
BspANI GGCC 5 cut(s) 185, 242, 372, 722, 805
BspCNI CTCAG 2 cut(s) 732, 829
BspFNI CGCG 1 cut(s) 200
BspLI GGNNCC 3 cut(s) 178, 460, 496
BspT104I TTCGAA 2 cut(s) 377, 603
BspT107I GGYRCC 2 cut(s) 458, 494
BsrBI CCGCTC 2 cut(s) 307, 481
BsrFI RCCGGY 3 cut(s) 225, 449, 461
BsrI ACTGG 1 cut(s) 723
BssAI RCCGGY 3 cut(s) 225, 449, 461
BssECI CCNNGG 6 cut(s) 180, 243, 528, 537, 799, 800
BssNI GRCGYC 5 cut(s) 141, 339, 432, 447, 495
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 5 cut(s) 349, 385, 439, 646, 909
Bst6I CTCTTC 3 cut(s) 28, 773, 881
BstACI GRCGYC 5 cut(s) 141, 339, 432, 447, 495
BstBI TTCGAA 2 cut(s) 377, 603
BstC8I GCNNGC 4 cut(s) 227, 451, 483, 552
BstDEI CTNAG 2 cut(s) 740, 837
BstDSI CCRYGG 2 cut(s) 180, 528
BstF5I GGATG 6 cut(s) 340, 418, 664, 697, 702, 850
BstFNI CGCG 1 cut(s) 200
BstH2I RGCGCY 3 cut(s) 153, 226, 498
BstHHI GCGC 4 cut(s) 152, 202, 225, 497
BstMAI GTCTC 2 cut(s) 323, 473
BstMWI GCNNNNNNNGC 9 cut(s) 11, 158, 191, 194, 462, 533, 710, 719, 821
BstNI CCWGG 1 cut(s) 239
BstSCI CCNGG 3 cut(s) 237, 799, 800
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 2 cut(s) 440, 574
BstV2I GAAGAC 1 cut(s) 386
BsuRI GGCC 5 cut(s) 185, 242, 372, 722, 805
BtgI CCRYGG 2 cut(s) 180, 528
BtgZI GCGATG 1 cut(s) 142
BtsCI GGATG 6 cut(s) 340, 418, 664, 697, 702, 850
BtsIMutI CAGTG 1 cut(s) 716
Cac8I GCNNGC 4 cut(s) 227, 451, 483, 552
CfoI GCGC 4 cut(s) 152, 202, 225, 497
Cfr10I RCCGGY 3 cut(s) 225, 449, 461
Cfr13I GGNCC 2 cut(s) 419, 803
Cfr9I CCCGGG 1 cut(s) 800
CseI GACGC 2 cut(s) 421, 455
CviAII CATG 1 cut(s) 529
DdeI CTNAG 2 cut(s) 740, 837
DinI GGCGCC 1 cut(s) 496
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 3 cut(s) 183, 370, 720
Eam1104I CTCTTC 3 cut(s) 28, 773, 881
EarI CTCTTC 3 cut(s) 28, 773, 881
EciI GGCGGA 3 cut(s) 244, 511, 704
Eco130I CCWWGG 1 cut(s) 528
Eco32I GATATC 2 cut(s) 361, 772
Eco47I GGWCC 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 797
Eco88I CYCGRG 2 cut(s) 218, 800
EcoRII CCWGG 1 cut(s) 237
EcoRV GATATC 2 cut(s) 361, 772
EcoT14I CCWWGG 1 cut(s) 528
EgeI GGCGCC 1 cut(s) 496
EheI GGCGCC 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 528
Esp3I CGTCTC 1 cut(s) 323
FaeI CATG 1 cut(s) 532
FaiI YATR 3 cut(s) 56, 530, 639
FalI AAGNNNNNCTT 4 cut(s) 269, 301, 408, 440
FatI CATG 1 cut(s) 528
FauI CCCGC 4 cut(s) 88, 100, 129, 486
FblI GTMKAC 1 cut(s) 144
FokI GGATG 6 cut(s) 347, 425, 671, 704, 709, 857
GlaI GCGC 4 cut(s) 151, 201, 224, 496
GsuI CTGGAG 1 cut(s) 221
HaeII RGCGCY 3 cut(s) 153, 226, 498
HaeIII GGCC 5 cut(s) 185, 242, 372, 722, 805
HapII CCGG 4 cut(s) 226, 450, 462, 801
HgaI GACGC 2 cut(s) 421, 455
HhaI GCGC 4 cut(s) 152, 202, 225, 497
Hin1I GRCGYC 5 cut(s) 141, 339, 432, 447, 495
Hin1II CATG 1 cut(s) 532
Hin6I GCGC 4 cut(s) 150, 200, 223, 495
HinP1I GCGC 4 cut(s) 150, 200, 223, 495
HincII GTYRAC 3 cut(s) 145, 343, 408
HindII GTYRAC 3 cut(s) 145, 343, 408
HinfI GANTC 3 cut(s) 585, 667, 727
HpaII CCGG 4 cut(s) 226, 450, 462, 801
HphI GGTGA 2 cut(s) 427, 496
Hpy166II GTNNAC 3 cut(s) 145, 343, 408
Hpy188I TCNGA 3 cut(s) 354, 741, 838
Hpy188III TCNNGA 3 cut(s) 388, 656, 775
Hpy8I GTNNAC 3 cut(s) 145, 343, 408
Hpy99I CGWCG 5 cut(s) 134, 143, 146, 389, 820
HpyAV CCTTC 5 cut(s) 410, 485, 623, 816, 859
HpyCH4III ACNGT 5 cut(s) 349, 385, 439, 646, 909
HpyCH4IV ACGT 2 cut(s) 141, 339
HpyCH4V TGCA 3 cut(s) 713, 749, 832
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 158, 191, 194, 462, 533, 710, 719, 821
HpyF3I CTNAG 2 cut(s) 740, 837
HpySE526I ACGT 2 cut(s) 141, 339
Hsp92I GRCGYC 5 cut(s) 141, 339, 432, 447, 495
Hsp92II CATG 1 cut(s) 532
HspAI GCGC 4 cut(s) 150, 200, 223, 495
KasI GGCGCC 1 cut(s) 494
KroI GCCGGC 2 cut(s) 225, 449
KroNI GCCGGC 2 cut(s) 227, 451
LmnI GCTCC 2 cut(s) 182, 240
Lsp1109I GCAGC 2 cut(s) 440, 574
LweI GCATC 1 cut(s) 722
MaeII ACGT 2 cut(s) 141, 339
MaeIII GTNAC 3 cut(s) 433, 443, 502
MbiI CCGCTC 2 cut(s) 307, 481
MboII GAAGA 9 cut(s) 15, 325, 328, 391, 412, 787, 790, 874, 898
MluCI AATT 1 cut(s) 599
Mly113I GGCGCC 1 cut(s) 495
MlyI GAGTC 1 cut(s) 594
MreI CGCCGGCG 1 cut(s) 225
MroNI GCCGGC 2 cut(s) 225, 449
MseI TTAA 1 cut(s) 946
MslI CAYNNNNRTG 1 cut(s) 647
MspI CCGG 4 cut(s) 226, 450, 462, 801
MspR9I CCNGG 3 cut(s) 239, 801, 802
Mva1269I GAATGC 1 cut(s) 5
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 9 cut(s) 11, 158, 191, 194, 462, 533, 710, 719, 821
NaeI GCCGGC 2 cut(s) 227, 451
NarI GGCGCC 1 cut(s) 495
NciI CCSGG 2 cut(s) 801, 802
NcoI CCATGG 1 cut(s) 528
NgoMIV GCCGGC 2 cut(s) 225, 449
NlaIII CATG 1 cut(s) 532
NlaIV GGNNCC 3 cut(s) 178, 460, 496
NmeAIII GCCGAG 4 cut(s) 268, 348, 562, 748
NmuCI GTSAC 3 cut(s) 433, 443, 502
NspV TTCGAA 2 cut(s) 377, 603
OliI CACNNNNGTG 1 cut(s) 647
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 216, 258
PctI GAATGC 1 cut(s) 5
PdiI GCCGGC 2 cut(s) 227, 451
PfeI GAWTC 2 cut(s) 667, 727
PleI GAGTC 1 cut(s) 593
PluTI GGCGCC 1 cut(s) 498
PpsI GAGTC 1 cut(s) 593
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 3 cut(s) 178, 460, 496
PspPI GGNCC 2 cut(s) 419, 803
RseI CAYNNNNRTG 1 cut(s) 647
SalI GTCGAC 1 cut(s) 143
SaqAI TTAA 1 cut(s) 946
Sau96I GGNCC 2 cut(s) 419, 803
SchI GAGTC 1 cut(s) 594
ScrFI CCNGG 3 cut(s) 239, 801, 802
SfaNI GCATC 1 cut(s) 722
SfoI GGCGCC 1 cut(s) 496
SfuI TTCGAA 2 cut(s) 377, 603
SgrAI CRCCGGYG 1 cut(s) 225
SinI GGWCC 1 cut(s) 419
SmaI CCCGGG 1 cut(s) 802
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 1 cut(s) 599
SspDI GGCGCC 1 cut(s) 494
StyD4I CCNGG 3 cut(s) 237, 799, 800
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 5 cut(s) 349, 385, 439, 646, 909
TaiI ACGT 2 cut(s) 144, 342
TaqI TCGA 6 cut(s) 144, 303, 377, 387, 603, 920
TasI AATT 1 cut(s) 599
TfiI GAWTC 2 cut(s) 667, 727
Tru1I TTAA 1 cut(s) 946
Tru9I TTAA 1 cut(s) 946
TscAI CASTG 1 cut(s) 723
TseFI GTSAC 3 cut(s) 433, 443, 502
TseI GCWGC 2 cut(s) 453, 562
Tsp45I GTSAC 3 cut(s) 433, 443, 502
TspDTI ATGAA 4 cut(s) 413, 429, 504, 659
TspGWI ACGGA 4 cut(s) 118, 169, 347, 425
TspMI CCCGGG 1 cut(s) 800
TspRI CASTG 1 cut(s) 723
VpaK11BI GGWCC 1 cut(s) 419
XapI RAATTY 1 cut(s) 599
XmaI CCCGGG 1 cut(s) 800
XmiI GTMKAC 1 cut(s) 144
ZraI GACGTC 2 cut(s) 142, 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.