Rw7G008470

Magnesium protoporphyrin IX methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009399
Physical Location & Seq
Reverse (-)
7714335 .. 7714995
661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G008470.1

Sequence Viewer

Length: 552 bp
ATGAAGATGTTGACGGATGAAGGTCCGCTTGGTGGCGTCACCGTCTGTGACGCCGGCTGCGGCACCGGCTGCCTCGCTATCCCGCTCGCTAATGAAGGCGCCCTCGTCACCGCCACCGACATTTCCGCCGCCATGGTCGCCGAGGCAGAGAAGCAGGCAAGAGGGCAGCTTGATTTGGGCAAAGAGGGAGTCGTATTGCCGAAATTCGAAGTGAGTGATTTAGAGAGCTTGGAAGGGAAGTATGACACGGTGGTGTGTCTGGATGTGTTGATTCATTACCCACAGAGCAAGGCGGATGGGATGATTGCCCACCTTGCATCACTGGCTGAGAATCGGTTGCTTCTGAGCTTTGCACCCAAGACATTGTATTACGATATCCTGAAGAGGATAGGAGAGTTCTTCCCCGGGCCTTCAAAAGCCACGAGGGCGTATCTGCACTCTGAGGCCGATGTGGAGAGGGCTTTGAAGAAGGTTGGGTGGAGGATAAGGAAGAGAGGACTAACAACAACACAGTTCTATTTTTCGAGGCTCGTTGAGGCTGTTCCTATTTAA

Protein Analysis

183

Amino Acids

19.83

Weight (kDa)

5.95

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 12 - 95 2.4e-09 Methyltransferase domain
Methyltransf_23 PF13489 14 - 163 6.9e-11 Methyltransferase domain
Methyltransf_25 PF13649 15 - 107 4.8e-13 Methyltransferase domain
Methyltransf_12 PF08242 17 - 108 5.3e-12 Methyltransferase domain
Methyltransf_11 PF08241 17 - 94 3.9e-10 Methyltransferase domain
Mg-por_mtran_C PF07109 86 - 181 2.5e-37 Magnesium-protoporphyrin IX methyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009399)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25080 AT4G25080 AT4G25080 AT4G25080 AT4G25080 AT4G25080
fragaria_vesca FvH4_5g09430 FvH4_5g09430
malus_domestica MD06G1131500.v1.1 MD14G1147900.v1.1
prunus_persica Prupe.5G142500_v2.0.a1
pyrus_communis pycom06g12290
rosa_chinensis RchiOBHm_Chr7g0188761
rosa_laevigata RLG00000004663
rosa_multiflora Rmu_sc0042908.1_g000002
rosa_roxburghii Rroxscaffold_3G00266060
rosa_rugosa Rorug06G0493700
rosa_samantha Rh7AG098100 Rh7BG099800 Rh7CG101500 Rh7DG099900
rosa_wichuraiana Rw7G008470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 62, 98
AccBSI CCGCTC 1 cut(s) 85
AciI CCGC 7 cut(s) 26, 60, 83, 111, 126, 129, 293
AcsI RAATTY 1 cut(s) 203
AcuI CTGAAG 1 cut(s) 401
AcyI GRCGYC 3 cut(s) 36, 51, 99
AfiI CCNNNNNNNGG 1 cut(s) 32
AgsI TTSAA 2 cut(s) 414, 466
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AleI CACNNNNGTG 1 cut(s) 251
AluBI AGCT 3 cut(s) 169, 228, 348
AluI AGCT 3 cut(s) 169, 228, 348
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 2 cut(s) 407, 444
ApeKI GCWGC 3 cut(s) 57, 69, 166
ApoI RAATTY 1 cut(s) 203
ArsI GACNNNNNNTTYG 2 cut(s) 174, 206
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 2 cut(s) 23, 407
AsuC2I CCSGG 2 cut(s) 405, 406
AsuHPI GGTGA 2 cut(s) 31, 100
AsuII TTCGAA 1 cut(s) 207
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 23
BanI GGYRCC 2 cut(s) 62, 98
BauI CACGAG 1 cut(s) 421
BbvI GCAGC 3 cut(s) 44, 56, 178
BccI CCATC 1 cut(s) 290
BcnI CCSGG 2 cut(s) 405, 406
BfoI RGCGCY 1 cut(s) 102
BglI GCCNNNNNGGC 1 cut(s) 425
BisI GCNGC 5 cut(s) 58, 61, 70, 129, 167
BlsI GCNGC 5 cut(s) 59, 62, 71, 130, 168
Bme1390I CCNGG 2 cut(s) 405, 406
Bme18I GGWCC 1 cut(s) 23
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 2 cut(s) 23, 407
BmiI GGNNCC 2 cut(s) 64, 100
BmrFI CCNGG 2 cut(s) 405, 406
BmsI GCATC 1 cut(s) 326
Bpu14I TTCGAA 1 cut(s) 207
BpuMI CCSGG 2 cut(s) 405, 406
BsaHI GRCGYC 3 cut(s) 36, 51, 99
BsaJI CCNNGG 4 cut(s) 132, 141, 403, 404
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse118I RCCGGY 2 cut(s) 53, 65
Bse1I ACTGG 1 cut(s) 327
BseDI CCNNGG 4 cut(s) 132, 141, 403, 404
BseGI GGATG 4 cut(s) 22, 268, 301, 306
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 3 cut(s) 318, 335, 432
BseNI ACTGG 1 cut(s) 327
BseXI GCAGC 3 cut(s) 44, 56, 178
BsgI GTGCAG 1 cut(s) 419
BshFI GGCC 2 cut(s) 409, 446
BshNI GGYRCC 2 cut(s) 62, 98
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 3 cut(s) 54, 66, 405
BslI CCNNNNNNNGG 1 cut(s) 32
BsnI GGCC 2 cut(s) 409, 446
BsoBI CYCGRG 1 cut(s) 404
Bsp119I TTCGAA 1 cut(s) 207
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 7 cut(s) 26, 60, 83, 111, 126, 129, 293
BspANI GGCC 2 cut(s) 409, 446
BspCNI CTCAG 3 cut(s) 319, 336, 433
BspLI GGNNCC 2 cut(s) 64, 100
BspT104I TTCGAA 1 cut(s) 207
BspT107I GGYRCC 2 cut(s) 62, 98
BsrBI CCGCTC 1 cut(s) 85
BsrFI RCCGGY 2 cut(s) 53, 65
BsrI ACTGG 1 cut(s) 327
BssAI RCCGGY 2 cut(s) 53, 65
BssECI CCNNGG 4 cut(s) 132, 141, 403, 404
BssNI GRCGYC 3 cut(s) 36, 51, 99
BssSI CACGAG 1 cut(s) 421
BssT1I CCWWGG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 421
Bst4CI ACNGT 3 cut(s) 43, 250, 513
Bst6I CTCTTC 2 cut(s) 377, 485
BstACI GRCGYC 3 cut(s) 36, 51, 99
BstAPI GCANNNNNTGC 1 cut(s) 69
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 3 cut(s) 55, 87, 156
BstDEI CTNAG 3 cut(s) 327, 344, 441
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 4 cut(s) 22, 268, 301, 306
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 1 cut(s) 101
BstMWI GCNNNNNNNGC 6 cut(s) 66, 69, 137, 314, 323, 425
BstSCI CCNGG 2 cut(s) 403, 404
BstV1I GCAGC 3 cut(s) 44, 56, 178
BsuRI GGCC 2 cut(s) 409, 446
BtgI CCRYGG 1 cut(s) 132
BtsCI GGATG 4 cut(s) 22, 268, 301, 306
BtsIMutI CAGTG 1 cut(s) 320
Cac8I GCNNGC 3 cut(s) 55, 87, 156
CfoI GCGC 1 cut(s) 101
Cfr10I RCCGGY 2 cut(s) 53, 65
Cfr13I GGNCC 2 cut(s) 23, 407
Cfr9I CCCGGG 1 cut(s) 404
CseI GACGC 2 cut(s) 25, 59
CviAII CATG 1 cut(s) 133
DdeI CTNAG 3 cut(s) 327, 344, 441
DinI GGCGCC 1 cut(s) 100
Eam1104I CTCTTC 2 cut(s) 377, 485
EarI CTCTTC 2 cut(s) 377, 485
EciI GGCGGA 2 cut(s) 115, 308
Eco130I CCWWGG 1 cut(s) 132
Eco32I GATATC 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 23
Eco57I CTGAAG 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 404
EcoRV GATATC 1 cut(s) 376
EcoT14I CCWWGG 1 cut(s) 132
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 1 cut(s) 136
FaiI YATR 2 cut(s) 134, 243
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FatI CATG 1 cut(s) 132
FauI CCCGC 1 cut(s) 90
Fnu4HI GCNGC 5 cut(s) 58, 61, 70, 129, 167
FokI GGATG 4 cut(s) 29, 275, 308, 313
Fsp4HI GCNGC 5 cut(s) 58, 61, 70, 129, 167
GlaI GCGC 1 cut(s) 100
GluI GCNGC 5 cut(s) 58, 61, 70, 129, 167
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 2 cut(s) 409, 446
HapII CCGG 3 cut(s) 54, 66, 405
HgaI GACGC 2 cut(s) 25, 59
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 3 cut(s) 36, 51, 99
Hin1II CATG 1 cut(s) 136
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HinfI GANTC 3 cut(s) 189, 271, 331
HpaII CCGG 3 cut(s) 54, 66, 405
HphI GGTGA 2 cut(s) 31, 100
Hpy166II GTNNAC 1 cut(s) 12
Hpy188I TCNGA 2 cut(s) 345, 442
Hpy188III TCNNGA 2 cut(s) 260, 379
Hpy8I GTNNAC 1 cut(s) 12
HpyAV CCTTC 5 cut(s) 14, 89, 227, 420, 463
HpyCH4III ACNGT 3 cut(s) 43, 250, 513
HpyCH4V TGCA 3 cut(s) 317, 353, 436
HpyF10VI GCNNNNNNNGC 6 cut(s) 66, 69, 137, 314, 323, 425
HpyF3I CTNAG 3 cut(s) 327, 344, 441
Hsp92I GRCGYC 3 cut(s) 36, 51, 99
Hsp92II CATG 1 cut(s) 136
HspAI GCGC 1 cut(s) 99
KasI GGCGCC 1 cut(s) 98
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
LpnPI CCDG 7 cut(s) 67, 79, 140, 245, 308, 392, 418
Lsp1109I GCAGC 3 cut(s) 44, 56, 178
LweI GCATC 1 cut(s) 326
MaeIII GTNAC 3 cut(s) 37, 47, 106
MbiI CCGCTC 1 cut(s) 85
MboII GAAGA 5 cut(s) 16, 391, 394, 478, 502
MluCI AATT 1 cut(s) 203
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 1 cut(s) 198
MroNI GCCGGC 1 cut(s) 53
MseI TTAA 1 cut(s) 550
MslI CAYNNNNRTG 1 cut(s) 251
MspI CCGG 3 cut(s) 54, 66, 405
MspR9I CCNGG 2 cut(s) 405, 406
MwoI GCNNNNNNNGC 6 cut(s) 66, 69, 137, 314, 323, 425
NaeI GCCGGC 1 cut(s) 55
NarI GGCGCC 1 cut(s) 99
NciI CCSGG 2 cut(s) 405, 406
NcoI CCATGG 1 cut(s) 132
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 1 cut(s) 136
NlaIV GGNNCC 2 cut(s) 64, 100
NmeAIII GCCGAG 1 cut(s) 166
NmuCI GTSAC 3 cut(s) 37, 47, 106
NspV TTCGAA 1 cut(s) 207
OliI CACNNNNGTG 1 cut(s) 251
PdiI GCCGGC 1 cut(s) 55
PfeI GAWTC 2 cut(s) 271, 331
PkrI GCNGC 5 cut(s) 59, 62, 71, 130, 168
PleI GAGTC 1 cut(s) 197
PluTI GGCGCC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 197
PspN4I GGNNCC 2 cut(s) 64, 100
PspPI GGNCC 2 cut(s) 23, 407
RseI CAYNNNNRTG 1 cut(s) 251
SaqAI TTAA 1 cut(s) 550
SatI GCNGC 5 cut(s) 58, 61, 70, 129, 167
Sau96I GGNCC 2 cut(s) 23, 407
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 2 cut(s) 405, 406
SetI ASST 6 cut(s) 25, 171, 230, 315, 350, 474
SfaNI GCATC 1 cut(s) 326
SfoI GGCGCC 1 cut(s) 100
SfuI TTCGAA 1 cut(s) 207
SinI GGWCC 1 cut(s) 23
SmaI CCCGGG 1 cut(s) 406
SmiMI CAYNNNNRTG 1 cut(s) 251
Sse9I AATT 1 cut(s) 203
SsiI CCGC 7 cut(s) 26, 60, 83, 111, 126, 129, 293
SspDI GGCGCC 1 cut(s) 98
StyD4I CCNGG 2 cut(s) 403, 404
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 3 cut(s) 43, 250, 513
TaqI TCGA 2 cut(s) 207, 524
TasI AATT 1 cut(s) 203
TauI GCSGC 2 cut(s) 63, 131
TfiI GAWTC 2 cut(s) 271, 331
Tru1I TTAA 1 cut(s) 550
Tru9I TTAA 1 cut(s) 550
TscAI CASTG 1 cut(s) 327
TseFI GTSAC 3 cut(s) 37, 47, 106
TseI GCWGC 3 cut(s) 57, 69, 166
Tsp45I GTSAC 3 cut(s) 37, 47, 106
TspDTI ATGAA 4 cut(s) 17, 33, 108, 263
TspGWI ACGGA 1 cut(s) 29
TspMI CCCGGG 1 cut(s) 404
TspRI CASTG 1 cut(s) 327
VpaK11BI GGWCC 1 cut(s) 23
XapI RAATTY 1 cut(s) 203
XmaI CCCGGG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.