AT4G25720

Glutamine cyclotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007377
Physical Location & Seq
Reverse (-)
13099812 .. 13102594
2783 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25720.2

Sequence Viewer

Length: 963 bp
ATGGCGACTAGATCGCCTTATAAGAGACAAACGAAGCGATCCATGATTCAGTCTCTTCCAGCTTCTTCTTCTGCATCATCTCGCCGCCGATTCATTTCTCGGAAGAGGTTCGCGATGATGATTCCTCTTGCTCTCCTCTCCGGCGCCGTTTTCCTATTCTTTATGCCATTCAATAGCTGGGGCCAATCATCTGGTTCTTCTCTGGATCTCTCCCATAGGATCAATGAAATTGAAGTAGTTGCTGAGTTTCCTCATGATCCGGACGCCTTTACTCAGGGTCTTCTTTATGCGGGAAACGATACACTGTTCGAATCCACTGGTCTATACGGGAAGTCTTCAGTGAGGAAAGTGGATCTACGAACAGGAAAGGTTGAAATTCTTGAAAAAATGGACAATACATATTTTGGAGAGGGTTTAACACTCCTTGGAGAAAGGCTCTTTCAAGTCGCATGGTTGACAAACACGGGTTTCACATATGACCTTCGCAACTTAAGTAAAGTAAAACCATTTAAACATCATATGAAAGATGGATGGGGACTGGCTACCGATGGAAAAGCGTTATTTGGAAGTGATGGCACTTCTACATTATATCGGATGGACCCTCAAACAATGAAAGTTACTGATAAGCATATAGTCAGATATAATGGCCGTGAGGTACGTTACCTTAATGAGCTCGAGTACATAAACAACGAAGTTTGGGCAAATGTGTGGCAGTCTGATTGTATTGCTAGAATTTCACCCAAGGACGGATCTCTGCTTGGATGGATACTGCTTTCAAAATTAAGTCGGGGATTGCTGAAATCTGGACATAGGGGTATTGATGTTTTGAATGGCATAGCATGGGACAGTGACAAGCAACGCCTCTTTGTAACGGGGAAACTGTGGCCAAAGCTGTACCAAATCAAGCTAAAACAAGCATCAGCAAAGAGCGGAAATTATATCGAGCAACAATGCTTGGTATGA

Protein Analysis

320

Amino Acids

36.27

Weight (kDa)

9.69

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glu_cyclase_2 PF05096 72 - 289 1.3e-80 Glutamine cyclotransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 21
AccB1I GGYRCC 1 cut(s) 143
AccBSI CCGCTC 1 cut(s) 930
AccII CGCG 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 259
AciI CCGC 3 cut(s) 85, 290, 930
AclWI GGATC 6 cut(s) 33, 213, 227, 251, 360, 757
AcoI YGGCCR 2 cut(s) 646, 884
AcsI RAATTY 2 cut(s) 375, 732
AcuI CTGAAG 1 cut(s) 321
AcyI GRCGYC 2 cut(s) 144, 264
AfaI GTAC 3 cut(s) 657, 680, 896
AfiI CCNNNNNNNGG 1 cut(s) 746
AflII CTTAAG 1 cut(s) 490
AgsI TTSAA 7 cut(s) 172, 233, 374, 383, 443, 777, 829
AluBI AGCT 5 cut(s) 62, 177, 673, 892, 907
AluI AGCT 5 cut(s) 62, 177, 673, 892, 907
Alw21I GWGCWC 1 cut(s) 675
Alw26I GTCTC 2 cut(s) 19, 57
AlwI GGATC 6 cut(s) 33, 213, 227, 251, 360, 757
Ama87I CYCGRG 1 cut(s) 674
Aor13HI TCCGGA 1 cut(s) 259
AoxI GGCC 3 cut(s) 181, 646, 884
ApoI RAATTY 2 cut(s) 375, 732
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 146
AspS9I GGNCC 2 cut(s) 181, 598
AsuHPI GGTGA 1 cut(s) 729
AsuII TTCGAA 1 cut(s) 309
AvaI CYCGRG 1 cut(s) 674
AvaII GGWCC 1 cut(s) 598
BalI TGGCCA 1 cut(s) 886
BanI GGYRCC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 675
BbsI GAAGAC 2 cut(s) 272, 327
Bbv12I GWGCWC 1 cut(s) 675
BccI CCATC 6 cut(s) 521, 525, 542, 566, 589, 756
BceAI ACGGC 2 cut(s) 131, 633
BciVI GTATCC 1 cut(s) 759
BcoDI GTCTC 2 cut(s) 19, 57
BfaI CTAG 2 cut(s) 9, 729
BfoI RGCGCY 1 cut(s) 147
BfrI CTTAAG 1 cut(s) 490
BfuI GTATCC 1 cut(s) 759
BisI GCNGC 1 cut(s) 85
BlsI GCNGC 1 cut(s) 86
Bme18I GGWCC 1 cut(s) 598
BmeT110I CYCGRG 1 cut(s) 674
BmgT120I GGNCC 2 cut(s) 181, 598
BmiI GGNNCC 3 cut(s) 145, 182, 600
BmsI GCATC 2 cut(s) 83, 926
BpiI GAAGAC 2 cut(s) 272, 327
BplI GAGNNNNNCTC 2 cut(s) 420, 452
Bpu14I TTCGAA 1 cut(s) 309
BsaHI GRCGYC 2 cut(s) 144, 264
BsaJI CCNNGG 2 cut(s) 424, 741
BsaWI WCCGGW 1 cut(s) 259
Bsc4I CCNNNNNNNGG 1 cut(s) 746
Bse1I ACTGG 2 cut(s) 322, 543
BseAI TCCGGA 1 cut(s) 259
BseDI CCNNGG 2 cut(s) 424, 741
BseGI GGATG 3 cut(s) 536, 600, 767
BseLI CCNNNNNNNGG 1 cut(s) 746
BseMII CTCAG 2 cut(s) 234, 287
BseNI ACTGG 2 cut(s) 322, 543
BseRI GAGGAG 1 cut(s) 125
BseYI CCCAGC 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 3 cut(s) 183, 648, 886
BshNI GGYRCC 1 cut(s) 143
BsiHKAI GWGCWC 1 cut(s) 675
BsiHKCI CYCGRG 1 cut(s) 674
BsiSI CCGG 2 cut(s) 141, 260
BslFI GGGAC 2 cut(s) 549, 857
BslI CCNNNNNNNGG 1 cut(s) 746
BsmAI GTCTC 2 cut(s) 19, 57
BsmFI GGGAC 2 cut(s) 549, 857
BsnI GGCC 3 cut(s) 183, 648, 886
BsoBI CYCGRG 1 cut(s) 674
Bsp119I TTCGAA 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 675
Bsp13I TCCGGA 1 cut(s) 259
Bsp143I GATC 7 cut(s) 11, 38, 205, 219, 256, 352, 749
Bsp68I TCGCGA 1 cut(s) 113
BspACI CCGC 3 cut(s) 85, 290, 930
BspANI GGCC 3 cut(s) 183, 648, 886
BspCNI CTCAG 2 cut(s) 235, 286
BspEI TCCGGA 1 cut(s) 259
BspFNI CGCG 1 cut(s) 113
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 3 cut(s) 145, 182, 600
BspPI GGATC 6 cut(s) 33, 213, 227, 251, 360, 757
BspT104I TTCGAA 1 cut(s) 309
BspT107I GGYRCC 1 cut(s) 143
BspTI CTTAAG 1 cut(s) 490
BsrBI CCGCTC 1 cut(s) 930
BsrI ACTGG 2 cut(s) 322, 543
BssECI CCNNGG 2 cut(s) 424, 741
BssMI GATC 7 cut(s) 11, 38, 205, 219, 256, 352, 749
BssNI GRCGYC 2 cut(s) 144, 264
BssT1I CCWWGG 2 cut(s) 424, 741
Bst4CI ACNGT 3 cut(s) 306, 848, 882
Bst6I CTCTTC 2 cut(s) 60, 98
BstACI GRCGYC 2 cut(s) 144, 264
BstAFI CTTAAG 1 cut(s) 490
BstBI TTCGAA 1 cut(s) 309
BstDEI CTNAG 2 cut(s) 243, 273
BstF5I GGATG 3 cut(s) 536, 600, 767
BstFNI CGCG 1 cut(s) 113
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 1 cut(s) 146
BstKTI GATC 7 cut(s) 14, 41, 208, 222, 259, 355, 752
BstMAI GTCTC 2 cut(s) 19, 57
BstMBI GATC 7 cut(s) 11, 38, 205, 219, 256, 352, 749
BstUI CGCG 1 cut(s) 113
BstV2I GAAGAC 2 cut(s) 272, 327
BstX2I RGATCY 3 cut(s) 205, 352, 749
BstXI CCANNNNNNTGG 1 cut(s) 191
BstYI RGATCY 3 cut(s) 205, 352, 749
BsuI GTATCC 1 cut(s) 759
BsuRI GGCC 3 cut(s) 183, 648, 886
BtgZI GCGATG 1 cut(s) 128
BtsCI GGATG 3 cut(s) 536, 600, 767
BtsIMutI CAGTG 4 cut(s) 302, 315, 345, 853
BtuMI TCGCGA 1 cut(s) 113
CciI TCATGA 1 cut(s) 253
CfoI GCGC 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 181, 598
CseI GACGC 1 cut(s) 272
Csp6I GTAC 3 cut(s) 656, 679, 895
CviAII CATG 4 cut(s) 43, 254, 450, 840
CviQI GTAC 3 cut(s) 656, 679, 895
DdeI CTNAG 2 cut(s) 243, 273
DinI GGCGCC 1 cut(s) 145
DpnI GATC 7 cut(s) 13, 40, 207, 221, 258, 354, 751
DpnII GATC 7 cut(s) 11, 38, 205, 219, 256, 352, 749
DraI TTTAAA 1 cut(s) 511
EaeI YGGCCR 2 cut(s) 646, 884
Eam1104I CTCTTC 2 cut(s) 60, 98
EarI CTCTTC 2 cut(s) 60, 98
Ecl136II GAGCTC 1 cut(s) 673
Eco130I CCWWGG 2 cut(s) 424, 741
Eco24I GRGCYC 1 cut(s) 675
Eco47I GGWCC 1 cut(s) 598
Eco53kI GAGCTC 1 cut(s) 673
Eco57I CTGAAG 1 cut(s) 321
Eco88I CYCGRG 1 cut(s) 674
EcoICRI GAGCTC 1 cut(s) 673
EcoT14I CCWWGG 2 cut(s) 424, 741
EcoT38I GRGCYC 1 cut(s) 675
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
ErhI CCWWGG 2 cut(s) 424, 741
FaeI CATG 4 cut(s) 46, 257, 453, 843
FaqI GGGAC 2 cut(s) 549, 857
FatI CATG 4 cut(s) 42, 253, 449, 839
FauI CCCGC 1 cut(s) 283
FauNDI CATATG 2 cut(s) 475, 519
Fnu4HI GCNGC 1 cut(s) 85
FokI GGATG 3 cut(s) 543, 607, 774
FriOI GRGCYC 1 cut(s) 675
Fsp4HI GCNGC 1 cut(s) 85
FspBI CTAG 2 cut(s) 9, 729
GlaI GCGC 1 cut(s) 145
GluI GCNGC 1 cut(s) 85
GsaI CCCAGC 1 cut(s) 181
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 3 cut(s) 183, 648, 886
HapII CCGG 2 cut(s) 141, 260
HgaI GACGC 1 cut(s) 272
HhaI GCGC 1 cut(s) 146
Hin1I GRCGYC 2 cut(s) 144, 264
Hin1II CATG 4 cut(s) 46, 257, 453, 843
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HincII GTYRAC 1 cut(s) 456
HindII GTYRAC 1 cut(s) 456
HinfI GANTC 4 cut(s) 46, 90, 121, 311
HpaII CCGG 2 cut(s) 141, 260
HphI GGTGA 1 cut(s) 729
Hpy166II GTNNAC 1 cut(s) 456
Hpy188I TCNGA 4 cut(s) 102, 594, 638, 718
Hpy188III TCNNGA 6 cut(s) 112, 203, 254, 260, 380, 804
Hpy8I GTNNAC 1 cut(s) 456
HpyAV CCTTC 1 cut(s) 491
HpyCH4III ACNGT 3 cut(s) 306, 848, 882
HpyCH4IV ACGT 1 cut(s) 658
HpyCH4V TGCA 1 cut(s) 74
HpyF3I CTNAG 2 cut(s) 243, 273
HpySE526I ACGT 1 cut(s) 658
Hsp92I GRCGYC 2 cut(s) 144, 264
Hsp92II CATG 4 cut(s) 46, 257, 453, 843
HspAI GCGC 1 cut(s) 144
KasI GGCGCC 1 cut(s) 143
Kpn2I TCCGGA 1 cut(s) 259
Kzo9I GATC 7 cut(s) 11, 38, 205, 219, 256, 352, 749
LweI GCATC 2 cut(s) 83, 926
MaeI CTAG 2 cut(s) 9, 729
MaeII ACGT 1 cut(s) 658
MaeIII GTNAC 4 cut(s) 616, 659, 848, 868
MalI GATC 7 cut(s) 13, 40, 207, 221, 258, 354, 751
MbiI CCGCTC 1 cut(s) 930
MboI GATC 7 cut(s) 11, 38, 205, 219, 256, 352, 749
MboII GAAGA 7 cut(s) 47, 57, 60, 115, 189, 272, 327
MflI RGATCY 3 cut(s) 205, 352, 749
MhlI GDGCHC 1 cut(s) 675
MlsI TGGCCA 1 cut(s) 886
MluCI AATT 5 cut(s) 228, 375, 732, 779, 934
MluNI TGGCCA 1 cut(s) 886
Mly113I GGCGCC 1 cut(s) 144
MnlI CCTC 9 cut(s) 99, 135, 146, 261, 336, 403, 612, 646, 872
Mox20I TGGCCA 1 cut(s) 886
MroI TCCGGA 1 cut(s) 259
MroXI GAANNNNTTC 1 cut(s) 107
MscI TGGCCA 1 cut(s) 886
MseI TTAA 5 cut(s) 416, 491, 510, 666, 782
Msp20I TGGCCA 1 cut(s) 886
MspCI CTTAAG 1 cut(s) 490
MspI CCGG 2 cut(s) 141, 260
MvnI CGCG 1 cut(s) 113
NarI GGCGCC 1 cut(s) 144
NdeI CATATG 2 cut(s) 475, 519
NdeII GATC 7 cut(s) 11, 38, 205, 219, 256, 352, 749
NlaIII CATG 4 cut(s) 46, 257, 453, 843
NlaIV GGNNCC 3 cut(s) 145, 182, 600
NmuCI GTSAC 1 cut(s) 848
NruI TCGCGA 1 cut(s) 113
NspV TTCGAA 1 cut(s) 309
PaeR7I CTCGAG 1 cut(s) 674
PagI TCATGA 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 4 cut(s) 46, 90, 121, 311
PkrI GCNGC 1 cut(s) 86
PluTI GGCGCC 1 cut(s) 147
PsiI TTATAA 1 cut(s) 21
Psp124BI GAGCTC 1 cut(s) 675
PspFI CCCAGC 1 cut(s) 177
PspN4I GGNNCC 3 cut(s) 145, 182, 600
PspPI GGNCC 2 cut(s) 181, 598
PspXI VCTCGAGB 1 cut(s) 674
PsuI RGATCY 3 cut(s) 205, 352, 749
RruI TCGCGA 1 cut(s) 113
RsaI GTAC 3 cut(s) 657, 680, 896
RsaNI GTAC 3 cut(s) 656, 679, 895
SacI GAGCTC 1 cut(s) 675
SaqAI TTAA 5 cut(s) 416, 491, 510, 666, 782
SatI GCNGC 1 cut(s) 85
Sau3AI GATC 7 cut(s) 11, 38, 205, 219, 256, 352, 749
Sau96I GGNCC 2 cut(s) 181, 598
SduI GDGCHC 1 cut(s) 675
SfaNI GCATC 2 cut(s) 83, 926
SfoI GGCGCC 1 cut(s) 145
Sfr274I CTCGAG 1 cut(s) 674
SfuI TTCGAA 1 cut(s) 309
SinI GGWCC 1 cut(s) 598
SlaI CTCGAG 1 cut(s) 674
SmlI CTYRAG 2 cut(s) 490, 674
SmoI CTYRAG 2 cut(s) 490, 674
Sse9I AATT 5 cut(s) 228, 375, 732, 779, 934
SsiI CCGC 3 cut(s) 85, 290, 930
SspDI GGCGCC 1 cut(s) 143
SspMI CTAG 2 cut(s) 9, 729
SstI GAGCTC 1 cut(s) 675
StyI CCWWGG 2 cut(s) 424, 741
TaaI ACNGT 3 cut(s) 306, 848, 882
TaiI ACGT 1 cut(s) 661
TaqI TCGA 3 cut(s) 309, 675, 942
TasI AATT 5 cut(s) 228, 375, 732, 779, 934
TatI WGTACW 1 cut(s) 678
TauI GCSGC 1 cut(s) 87
TfiI GAWTC 4 cut(s) 46, 90, 121, 311
Tru1I TTAA 5 cut(s) 416, 491, 510, 666, 782
Tru9I TTAA 5 cut(s) 416, 491, 510, 666, 782
TscAI CASTG 4 cut(s) 309, 322, 345, 853
TseFI GTSAC 1 cut(s) 848
Tsp45I GTSAC 1 cut(s) 848
TspDTI ATGAA 4 cut(s) 82, 240, 536, 626
TspGWI ACGGA 1 cut(s) 762
TspRI CASTG 4 cut(s) 309, 322, 345, 853
Vha464I CTTAAG 1 cut(s) 490
VpaK11BI GGWCC 1 cut(s) 598
XapI RAATTY 2 cut(s) 375, 732
XcmI CCANNNNNNNNNTGG 1 cut(s) 174
XhoI CTCGAG 1 cut(s) 674
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 2 cut(s) 9, 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.