Rorug01G0408300

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002711
Physical Location & Seq
Reverse (-)
51437509 .. 51443802
6294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0408300.1

Sequence Viewer

Length: 1602 bp
ATGGGTCACAGAGAGAGCATCAAGTTTGATCTTCATTCAAATGGGTTTATAATCATTTTGATGCTAGTGTACTTAAACACCTCGGTGGATGCTTCGATCCATGAATACCAAGATGACACCTTTATACGTCGTTCCAATTCATTCTTCTTCCATGGCGGAAGTGAGGGTCTCTATGCTTCCAAAATTCACTCTGGTAATGACAAACTCGCTTCTGAAGACATGACCATTGATGGCAAGTCCTTTATCAGGTTTGAATCTATCACCTTTCGAAGGACCAAGGAGTCTGCTGAAAAGAAGAATGAGATGCAGCAGAGGACTGGCTTGGTTGAGGCTATAGTACTTGAGGTTAGAGACAGGGAGAAGATTGGGGGTTCTTATTTGAACTCTGAGGCAATATGCTGTACCCCGGAGCTTTCCAAGGATGGTTCGTGCAAGGTGGGAGAGGTTATCATCCAGCAGAAACCCGACAACCCTGATGGGCCAAAAAGGATGCAGACCTTCTTTGAGGGGAAAAATGAAGAGACTAACATGGAACTTCAGACTATTCAGATCAACAGCACTGGAATGTACTATCTTTATTTCATGTTTTGTGATCCAGAACTTGTTGGCACATCAATCAGTGGAAGAACTGTTTGGAGGAACCCGGATGGTTATTTACCTGGAAAGATGTCGCCGCTGATGACATTTTTCGGCCTCATGTCTTTAGCTTACCTTGTCCTTGGTCTTTTCTGGTTTCTCCGCTTTGTGCAGTATTGGAAAGATATAATACAGTTGCACTACCACATCAGTGCTGTTATTGGTCTTGGAATGTGTGAGATGGCTCTATGGTATTTTGAATATGCAAATTTCAATTCAACCGGAGCCAGGCCAATGGGAATTACCATGTGGGCAGTAACCTTCGGTGCTGTCAAAAAAACTGTTTCCCGGCTTCTTCTTCTGGTGGTTTCAATGGGGTATGGTGTTGTGCGGCCAACTCTTGGTGGCATTACCACAAAAGTACTACTCCTTGGTGTGATATACTTTGTAGCATCAGAAGCACTTGAGCTTGTTGAACATTTGGGAAATATCAATGACTTTTCTGGAAAAACAAGGCTTTTTCTGGTGCTACCGGTTGCTCTATTGGATGCCTGTTTTATTCTTTGGATCTTTTCATCACTATCTAAGACTTTGGAGAAGCTTCAGATTCGAAGAAGCATGGCCAAACTTGAGCTCTACCGGAAGTTTACCAATTCCCTTGCAGTATCAGTGTTGCTGTCTGTTGCTTGGATTGGTTATGAGTTGTATTTTAATGCGAGTGACCCGTTGAGTGAATTGTGGCGAAGAGCCTGGATCATCCCAGCTTTCTGGGTTTTGCTTGCATACTTTCTGTTGGTAGTTATATGTGTTCTCTGGGCTCCATCTCACAACCCAACCGGATATGCATACTCCGAGGAGACTGTGGATGACTTTGATGAGGAGGGTATCTCGCTCACTAGTGGAAGCGGAGTTAAGGTGGCTGGCATTGACTTGGCCAGCAAGCTAGAAAGAAAGGAAAGGAAGCTATCAAGTTCAGCAGATCACCATGTGTTTCTTGGGGAAGATGAGGAGGACAAGAGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

533

Amino Acids

60.37

Weight (kDa)

5.77

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 225 - 470 1.5e-93 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 50
AasI GACNNNNNNGTC 1 cut(s) 280
AccB7I CCANNNNNTGG 1 cut(s) 977
AciI CCGC 5 cut(s) 156, 674, 739, 967, 1482
AclWI GGATC 4 cut(s) 91, 587, 1151, 1337
AcoI YGGCCR 3 cut(s) 968, 1197, 1509
AcsI RAATTY 2 cut(s) 183, 844
AcuI CTGAAG 3 cut(s) 234, 521, 1163
AdeI CACNNNGTG 1 cut(s) 1564
AfaI GTAC 5 cut(s) 71, 339, 403, 569, 999
AfiI CCNNNNNNNGG 4 cut(s) 246, 270, 864, 977
AgeI ACCGGT 1 cut(s) 1108
AgsI TTSAA 8 cut(s) 39, 254, 382, 836, 850, 855, 948, 1052
AhlI ACTAGT 1 cut(s) 1472
AjnI CCWGG 3 cut(s) 658, 863, 1325
AjuI GAANNNNNNNTTGG 4 cut(s) 128, 160, 616, 648
AleI CACNNNNGTG 2 cut(s) 83, 786
AluBI AGCT 8 cut(s) 412, 707, 1045, 1177, 1210, 1340, 1519, 1540
AluI AGCT 8 cut(s) 412, 707, 1045, 1177, 1210, 1340, 1519, 1540
Alw21I GWGCWC 1 cut(s) 1212
Alw26I GTCTC 4 cut(s) 173, 345, 515, 1427
AlwI GGATC 4 cut(s) 91, 587, 1151, 1337
AoxI GGCC 6 cut(s) 479, 691, 866, 968, 1197, 1509
ApeKI GCWGC 1 cut(s) 307
ApoI RAATTY 2 cut(s) 183, 844
AsiGI ACCGGT 1 cut(s) 1108
AspS9I GGNCC 2 cut(s) 273, 479
AsuC2I CCSGG 3 cut(s) 407, 644, 925
AsuHPI GGTGA 2 cut(s) 253, 1550
AsuII TTCGAA 2 cut(s) 268, 1186
AvaII GGWCC 1 cut(s) 273
BalI TGGCCA 2 cut(s) 1199, 1511
BanII GRGCYC 2 cut(s) 1212, 1396
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 1212
BbvI GCAGC 1 cut(s) 319
BccI CCATC 6 cut(s) 224, 416, 470, 641, 811, 1405
BciT130I CCWGG 3 cut(s) 660, 865, 1327
BcnI CCSGG 3 cut(s) 407, 644, 925
BcoDI GTCTC 4 cut(s) 173, 345, 515, 1427
BcuI ACTAGT 1 cut(s) 1472
BfaI CTAG 3 cut(s) 65, 1473, 1520
BfmI CTRYAG 1 cut(s) 333
BisI GCNGC 3 cut(s) 308, 674, 968
BlsI GCNGC 3 cut(s) 309, 675, 969
BmcAI AGTACT 2 cut(s) 339, 999
Bme1390I CCNGG 6 cut(s) 407, 644, 660, 865, 925, 1327
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 2 cut(s) 273, 479
BmiI GGNNCC 3 cut(s) 641, 862, 1395
BmrFI CCNGG 6 cut(s) 407, 644, 660, 865, 925, 1327
BmsI GCATC 7 cut(s) 27, 51, 79, 294, 480, 1037, 1114
BpiI GAAGAC 1 cut(s) 222
BplI GAGNNNNNCTC 2 cut(s) 1448, 1480
Bpu14I TTCGAA 2 cut(s) 268, 1186
BpuEI CTTGAG 3 cut(s) 362, 1061, 1226
BpuMI CCSGG 3 cut(s) 407, 644, 925
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 8 cut(s) 81, 151, 276, 405, 417, 718, 1006, 1428
BsaWI WCCGGW 4 cut(s) 857, 1108, 1215, 1412
BsaXI ACNNNNNCTCC 2 cut(s) 1476, 1506
Bsc4I CCNNNNNNNGG 4 cut(s) 246, 270, 864, 977
Bse118I RCCGGY 1 cut(s) 1108
Bse1I ACTGG 2 cut(s) 322, 565
BseBI CCWGG 3 cut(s) 660, 865, 1327
BseDI CCNNGG 8 cut(s) 81, 151, 276, 405, 417, 718, 1006, 1428
BseGI GGATG 8 cut(s) 94, 427, 450, 495, 652, 1129, 1332, 1447
BseLI CCNNNNNNNGG 4 cut(s) 246, 270, 864, 977
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 2 cut(s) 322, 565
BseRI GAGGAG 3 cut(s) 1445, 1469, 1598
BseXI GCAGC 1 cut(s) 319
BseYI CCCAGC 1 cut(s) 1336
BsgI GTGCAG 1 cut(s) 767
BshFI GGCC 6 cut(s) 481, 693, 868, 970, 1199, 1511
BshTI ACCGGT 1 cut(s) 1108
BsiHKAI GWGCWC 1 cut(s) 1212
BsiSI CCGG 7 cut(s) 407, 644, 858, 925, 1109, 1216, 1413
BslI CCNNNNNNNGG 4 cut(s) 246, 270, 864, 977
BsmAI GTCTC 4 cut(s) 173, 345, 515, 1427
BsnI GGCC 6 cut(s) 481, 693, 868, 970, 1199, 1511
Bso31I GGTCTC 1 cut(s) 173
Bsp119I TTCGAA 2 cut(s) 268, 1186
Bsp1286I GDGCHC 2 cut(s) 1212, 1396
Bsp143I GATC 7 cut(s) 28, 96, 549, 592, 1143, 1329, 1555
Bsp19I CCATGG 1 cut(s) 151
BspACI CCGC 5 cut(s) 156, 674, 739, 967, 1482
BspANI GGCC 6 cut(s) 481, 693, 868, 970, 1199, 1511
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 3 cut(s) 641, 862, 1395
BspPI GGATC 4 cut(s) 91, 587, 1151, 1337
BspQI GCTCTTC 1 cut(s) 1315
BspT104I TTCGAA 2 cut(s) 268, 1186
BspTNI GGTCTC 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 1108
BsrI ACTGG 2 cut(s) 322, 565
BssAI RCCGGY 1 cut(s) 1108
BssECI CCNNGG 8 cut(s) 81, 151, 276, 405, 417, 718, 1006, 1428
BssMI GATC 7 cut(s) 28, 96, 549, 592, 1143, 1329, 1555
BssT1I CCWWGG 5 cut(s) 151, 276, 417, 718, 1006
Bst2UI CCWGG 3 cut(s) 660, 865, 1327
Bst4CI ACNGT 4 cut(s) 631, 771, 919, 1438
Bst6I CTCTTC 2 cut(s) 513, 1315
BstBI TTCGAA 2 cut(s) 268, 1186
BstC8I GCNNGC 4 cut(s) 1356, 1498, 1513, 1517
BstDEI CTNAG 2 cut(s) 387, 1161
BstDSI CCRYGG 1 cut(s) 151
BstENI CCTNNNNNAGG 2 cut(s) 244, 268
BstF5I GGATG 8 cut(s) 94, 427, 450, 495, 652, 1129, 1332, 1447
BstKTI GATC 7 cut(s) 31, 99, 552, 595, 1146, 1332, 1558
BstMAI GTCTC 4 cut(s) 173, 345, 515, 1427
BstMBI GATC 7 cut(s) 28, 96, 549, 592, 1143, 1329, 1555
BstMWI GCNNNNNNNGC 1 cut(s) 1034
BstNI CCWGG 3 cut(s) 660, 865, 1327
BstSCI CCNGG 6 cut(s) 405, 642, 658, 863, 923, 1325
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 1 cut(s) 319
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 1143
BstXI CCANNNNNNTGG 2 cut(s) 871, 1344
BstYI RGATCY 1 cut(s) 1143
BsuRI GGCC 6 cut(s) 481, 693, 868, 970, 1199, 1511
BtgI CCRYGG 1 cut(s) 151
BtsCI GGATG 8 cut(s) 94, 427, 450, 495, 652, 1129, 1332, 1447
BtsIMutI CAGTG 4 cut(s) 558, 625, 793, 1251
Cac8I GCNNGC 4 cut(s) 1356, 1498, 1513, 1517
Cfr10I RCCGGY 1 cut(s) 1108
Cfr13I GGNCC 2 cut(s) 273, 479
Csp6I GTAC 5 cut(s) 70, 338, 402, 568, 998
CspAI ACCGGT 1 cut(s) 1108
CviAII CATG 9 cut(s) 101, 152, 220, 529, 583, 697, 883, 1195, 1562
CviQI GTAC 5 cut(s) 70, 338, 402, 568, 998
DdeI CTNAG 2 cut(s) 387, 1161
DpnI GATC 7 cut(s) 30, 98, 551, 594, 1145, 1331, 1557
DpnII GATC 7 cut(s) 28, 96, 549, 592, 1143, 1329, 1555
DraIII CACNNNGTG 1 cut(s) 1564
DrdI GACNNNNNNGTC 1 cut(s) 280
DseDI GACNNNNNNGTC 1 cut(s) 280
EaeI YGGCCR 3 cut(s) 968, 1197, 1509
Eam1104I CTCTTC 2 cut(s) 513, 1315
EarI CTCTTC 2 cut(s) 513, 1315
EciI GGCGGA 1 cut(s) 171
Ecl136II GAGCTC 1 cut(s) 1210
Eco130I CCWWGG 5 cut(s) 151, 276, 417, 718, 1006
Eco24I GRGCYC 2 cut(s) 1212, 1396
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 1210
Eco57I CTGAAG 3 cut(s) 234, 521, 1163
EcoICRI GAGCTC 1 cut(s) 1210
EcoNI CCTNNNNNAGG 2 cut(s) 244, 268
EcoRII CCWGG 3 cut(s) 658, 863, 1325
EcoT14I CCWWGG 5 cut(s) 151, 276, 417, 718, 1006
EcoT22I ATGCAT 1 cut(s) 1423
EcoT38I GRGCYC 2 cut(s) 1212, 1396
ErhI CCWWGG 5 cut(s) 151, 276, 417, 718, 1006
FaeI CATG 9 cut(s) 104, 155, 223, 532, 586, 700, 886, 1198, 1565
FatI CATG 9 cut(s) 100, 151, 219, 528, 582, 696, 882, 1194, 1561
Fnu4HI GCNGC 3 cut(s) 308, 674, 968
FokI GGATG 8 cut(s) 101, 434, 437, 502, 659, 1136, 1319, 1454
FriOI GRGCYC 2 cut(s) 1212, 1396
Fsp4HI GCNGC 3 cut(s) 308, 674, 968
FspBI CTAG 3 cut(s) 65, 1473, 1520
GluI GCNGC 3 cut(s) 308, 674, 968
GsaI CCCAGC 1 cut(s) 1340
HaeIII GGCC 6 cut(s) 481, 693, 868, 970, 1199, 1511
HapII CCGG 7 cut(s) 407, 644, 858, 925, 1109, 1216, 1413
Hin1II CATG 9 cut(s) 104, 155, 223, 532, 586, 700, 886, 1198, 1565
HindIII AAGCTT 1 cut(s) 1175
HinfI GANTC 3 cut(s) 254, 281, 1183
HpaII CCGG 7 cut(s) 407, 644, 858, 925, 1109, 1216, 1413
HphI GGTGA 2 cut(s) 253, 1550
Hpy166II GTNNAC 2 cut(s) 70, 1224
Hpy188I TCNGA 7 cut(s) 214, 388, 540, 549, 1033, 1182, 1429
Hpy188III TCNNGA 2 cut(s) 596, 1080
Hpy8I GTNNAC 2 cut(s) 70, 1224
Hpy99I CGWCG 1 cut(s) 132
HpyAV CCTTC 3 cut(s) 264, 508, 907
HpyCH4III ACNGT 4 cut(s) 631, 771, 919, 1438
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 9 cut(s) 307, 432, 493, 748, 775, 842, 1238, 1358, 1421
HpyF10VI GCNNNNNNNGC 1 cut(s) 1034
HpyF3I CTNAG 2 cut(s) 387, 1161
HpySE526I ACGT 1 cut(s) 127
Hsp92II CATG 9 cut(s) 104, 155, 223, 532, 586, 700, 886, 1198, 1565
Kzo9I GATC 7 cut(s) 28, 96, 549, 592, 1143, 1329, 1555
LguI GCTCTTC 1 cut(s) 1315
LmnI GCTCC 3 cut(s) 409, 860, 1399
Lsp1109I GCAGC 1 cut(s) 319
LweI GCATC 7 cut(s) 27, 51, 79, 294, 480, 1037, 1114
MaeI CTAG 3 cut(s) 65, 1473, 1520
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 3 cut(s) 5, 892, 1295
MalI GATC 7 cut(s) 30, 98, 551, 594, 1145, 1331, 1557
MboI GATC 7 cut(s) 28, 96, 549, 592, 1143, 1329, 1555
MflI RGATCY 1 cut(s) 1143
MhlI GDGCHC 2 cut(s) 1212, 1396
MlsI TGGCCA 2 cut(s) 1199, 1511
MluCI AATT 7 cut(s) 136, 183, 844, 850, 876, 1228, 1310
MluNI TGGCCA 2 cut(s) 1199, 1511
MlyI GAGTC 1 cut(s) 290
Mox20I TGGCCA 2 cut(s) 1199, 1511
Mph1103I ATGCAT 1 cut(s) 1423
MscI TGGCCA 2 cut(s) 1199, 1511
MseI TTAA 3 cut(s) 74, 1287, 1488
MslI CAYNNNNRTG 5 cut(s) 39, 59, 83, 563, 786
Msp20I TGGCCA 2 cut(s) 1199, 1511
MspA1I CMGCKG 1 cut(s) 676
MspI CCGG 7 cut(s) 407, 644, 858, 925, 1109, 1216, 1413
MspR9I CCNGG 6 cut(s) 407, 644, 660, 865, 925, 1327
MvaI CCWGG 3 cut(s) 660, 865, 1327
MwoI GCNNNNNNNGC 1 cut(s) 1034
NciI CCSGG 3 cut(s) 407, 644, 925
NcoI CCATGG 1 cut(s) 151
NdeII GATC 7 cut(s) 28, 96, 549, 592, 1143, 1329, 1555
NlaIII CATG 9 cut(s) 104, 155, 223, 532, 586, 700, 886, 1198, 1565
NlaIV GGNNCC 3 cut(s) 641, 862, 1395
NmuCI GTSAC 2 cut(s) 5, 1295
NsiI ATGCAT 1 cut(s) 1423
NspV TTCGAA 2 cut(s) 268, 1186
OliI CACNNNNGTG 2 cut(s) 83, 786
PciSI GCTCTTC 1 cut(s) 1315
PfeI GAWTC 2 cut(s) 254, 1183
PflMI CCANNNNNTGG 1 cut(s) 977
PinAI ACCGGT 1 cut(s) 1108
PkrI GCNGC 3 cut(s) 309, 675, 969
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
PsiI TTATAA 1 cut(s) 50
Psp124BI GAGCTC 1 cut(s) 1212
Psp6I CCWGG 3 cut(s) 658, 863, 1325
PspFI CCCAGC 1 cut(s) 1336
PspGI CCWGG 3 cut(s) 658, 863, 1325
PspN4I GGNNCC 3 cut(s) 641, 862, 1395
PspPI GGNCC 2 cut(s) 273, 479
PsuI RGATCY 1 cut(s) 1143
RsaI GTAC 5 cut(s) 71, 339, 403, 569, 999
RsaNI GTAC 5 cut(s) 70, 338, 402, 568, 998
RseI CAYNNNNRTG 5 cut(s) 39, 59, 83, 563, 786
SacI GAGCTC 1 cut(s) 1212
SapI GCTCTTC 1 cut(s) 1315
SaqAI TTAA 3 cut(s) 74, 1287, 1488
SatI GCNGC 3 cut(s) 308, 674, 968
Sau3AI GATC 7 cut(s) 28, 96, 549, 592, 1143, 1329, 1555
Sau96I GGNCC 2 cut(s) 273, 479
ScaI AGTACT 2 cut(s) 339, 999
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 6 cut(s) 407, 644, 660, 865, 925, 1327
SduI GDGCHC 2 cut(s) 1212, 1396
SfaNI GCATC 7 cut(s) 27, 51, 79, 294, 480, 1037, 1114
SfcI CTRYAG 1 cut(s) 333
SfuI TTCGAA 2 cut(s) 268, 1186
SinI GGWCC 1 cut(s) 273
SmiMI CAYNNNNRTG 5 cut(s) 39, 59, 83, 563, 786
SmlI CTYRAG 3 cut(s) 341, 1040, 1205
SmoI CTYRAG 3 cut(s) 341, 1040, 1205
SpeI ACTAGT 1 cut(s) 1472
Sse9I AATT 7 cut(s) 136, 183, 844, 850, 876, 1228, 1310
SsiI CCGC 5 cut(s) 156, 674, 739, 967, 1482
SspMI CTAG 3 cut(s) 65, 1473, 1520
SstI GAGCTC 1 cut(s) 1212
StyD4I CCNGG 6 cut(s) 405, 642, 658, 863, 923, 1325
StyI CCWWGG 5 cut(s) 151, 276, 417, 718, 1006
TaaI ACNGT 4 cut(s) 631, 771, 919, 1438
TaiI ACGT 1 cut(s) 130
TaqI TCGA 3 cut(s) 95, 268, 1186
TasI AATT 7 cut(s) 136, 183, 844, 850, 876, 1228, 1310
TatI WGTACW 4 cut(s) 69, 337, 567, 997
TauI GCSGC 2 cut(s) 676, 970
TfiI GAWTC 2 cut(s) 254, 1183
Tru1I TTAA 3 cut(s) 74, 1287, 1488
Tru9I TTAA 3 cut(s) 74, 1287, 1488
TscAI CASTG 4 cut(s) 565, 625, 793, 1251
TseFI GTSAC 2 cut(s) 5, 1295
TseI GCWGC 1 cut(s) 307
Tsp45I GTSAC 2 cut(s) 5, 1295
TspDTI ATGAA 6 cut(s) 23, 117, 129, 531, 571, 1140
TspRI CASTG 4 cut(s) 565, 625, 793, 1251
Van91I CCANNNNNTGG 1 cut(s) 977
VpaK11BI GGWCC 1 cut(s) 273
XagI CCTNNNNNAGG 2 cut(s) 244, 268
XapI RAATTY 2 cut(s) 183, 844
XcmI CCANNNNNNNNNTGG 1 cut(s) 1568
XspI CTAG 3 cut(s) 65, 1473, 1520
ZrmI AGTACT 2 cut(s) 339, 999
Zsp2I ATGCAT 1 cut(s) 1423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.