FvH4_4g13870

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002711
Physical Location & Seq
Forward (+)
17383435 .. 17385833
2399 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13870.t1

Sequence Viewer

Length: 1410 bp
ATGGATAAAAAATCTTCAACAATGTCTGATGTTGCTATTGTTGTTATTCTGGGATTGGTATTGCTACAATCGGCAGAATGCAGAAAGATTCAGGAGGCTGTTACGTACTCCGCCATTGCGTGCCGGAAACACAGTGCTCTTTTGACCGATTTTGGAGCCGTTGGTGATGGAAAAATATTGAACACAAAGGCCTTCAAAGCTGCAGTGGATAAATTAAGCCAGTATGCATCCGATGGCGGAGCGCAGCTTATTGTGCCCCCAGGCAAATGGCTTACCGGGAGCTTCAACCTCACCAGCCACTTTACCCTTTTCCTTCATAAGGATGCTGTTATTCTAGCGTCTCAGGAGGAGTCCCAGTGGCCTCTTCTTCCGCCACTGCCTTCATACGGGCAAGGGAGAGATGCCCCTGGTGGAAGGTTTAGCAGTCTCATCTTCGGAACAAATCTTACCGATGTCGTAATTACTGGTAACAATGGCACCATTGATGGCCAAGGAGCTCCTTGGTGGGACAAGTTCGAAAAGGGTCTTTTGAATGTCACCCGACCCTATTTGCTTGAGATTATGTTCTCTAATCAGATCCAAATCTCCAATCTCACTTTTACTAATTCTCCATCCTGGCACATCCATCCCGTTTACAGCAGTAACATTACTATACAAGGCATCACTATCCTTGCACCAGTTGAGGTTCCTAACACGGATGGGATTAACCCCGACTCATGTTCCCAAACTCGAATTGAAGACTGTTACATTGTCTCCGGTGATGACTGCATTGCAGTGAAGAGTGGCTGGGATAACTACGGAATCAAATTTGGAATGCCGATGGAGCATCTTGTTATCAGGAGGCTTACCTGCATTTCACCTAAAAGTGCTACGGTTGCTCTAGGTAGCGAAATGTCTGGTGGAATTCGGGATGTTAGAGTTGAAGATGTCACAGCCATTAACACCGAATCCAGCATCAGGATCAAAACAGCCGTAGGGAGAGGGGCTTATGTCAAAGATATTTTTGTACGAAGAATGACGTTGAGCACTATGAAGTACGTTTTCTGGATGACAGGCGCCTACGGTTCACATCCAAGCGAGGGCTTTGATCCAAACGCACTTCCTGTTATTCAAAACATCAACTATAGAGACATTGTTGCTAAAAATGTCACTCACCCAGGACAGTTGGATGGAATTGCTAATGATCCTTTCAAGGGAATCTGCATATCTAATGCGACTATCACACTAACTGCGAAGCCAAAGAAAGTGCTGTGGAACTGCACTGATGTCGAGGGAATTACAAGCAATGTGACTCCAAAGCCTTGCGCTTTGTTGCCCGAACAGCAAAAACCAATTAATTGTGCTTTCCCTGAGGATAAGTTAGCCATTGAGGGTGTCAAGCTGATGACTTGTTCTGCAGCTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

470

Amino Acids

50.82

Weight (kDa)

6.71

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 142 - 424 6.8e-36 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 857
AccB1I GGYRCC 2 cut(s) 476, 1055
AciI CCGC 3 cut(s) 111, 237, 371
AclWI GGATC 4 cut(s) 571, 968, 1082, 1178
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 2 cut(s) 806, 903
AcyI GRCGYC 1 cut(s) 1056
AfaI GTAC 3 cut(s) 107, 1008, 1037
AfiI CCNNNNNNNGG 5 cut(s) 319, 386, 957, 1079, 1193
AgsI TTSAA 9 cut(s) 18, 181, 196, 286, 532, 737, 923, 1112, 1192
AjnI CCWGG 4 cut(s) 259, 406, 614, 1156
AluBI AGCT 6 cut(s) 200, 247, 282, 497, 1381, 1400
AluI AGCT 6 cut(s) 200, 247, 282, 497, 1381, 1400
Alw21I GWGCWC 3 cut(s) 139, 499, 1028
Alw26I GTCTC 4 cut(s) 345, 431, 757, 1122
AlwI GGATC 4 cut(s) 571, 968, 1082, 1178
AlwNI CAGNNNCTG 1 cut(s) 1403
AoxI GGCC 3 cut(s) 189, 359, 487
ApeKI GCWGC 3 cut(s) 200, 244, 1397
ApoI RAATTY 2 cut(s) 806, 903
AseI ATTAAT 1 cut(s) 1335
AspLEI GCGC 3 cut(s) 244, 1058, 1307
AsuC2I CCSGG 1 cut(s) 277
AsuHPI GGTGA 6 cut(s) 176, 283, 529, 770, 849, 1145
AsuII TTCGAA 1 cut(s) 516
AxyI CCTNAGG 1 cut(s) 1350
BaeGI GKGCMC 1 cut(s) 258
BalI TGGCCA 1 cut(s) 489
BanI GGYRCC 2 cut(s) 476, 1055
BanII GRGCYC 1 cut(s) 499
BbsI GAAGAC 1 cut(s) 744
Bbv12I GWGCWC 3 cut(s) 139, 499, 1028
BbvI GCAGC 2 cut(s) 187, 256
BccI CCATC 8 cut(s) 161, 227, 479, 619, 633, 692, 814, 1163
BceAI ACGGC 2 cut(s) 143, 956
BcgI CGANNNNNNTGC 2 cut(s) 1249, 1283
BciT130I CCWGG 4 cut(s) 261, 408, 616, 1158
BcnI CCSGG 1 cut(s) 277
BcoDI GTCTC 4 cut(s) 345, 431, 757, 1122
BfaI CTAG 2 cut(s) 335, 881
BfmI CTRYAG 3 cut(s) 201, 1123, 1395
BfoI RGCGCY 1 cut(s) 1059
BfuAI ACCTGC 1 cut(s) 857
BisI GCNGC 3 cut(s) 201, 245, 1398
BlsI GCNGC 3 cut(s) 202, 246, 1399
Bme1390I CCNGG 5 cut(s) 261, 277, 408, 616, 1158
BmiI GGNNCC 4 cut(s) 157, 478, 687, 1057
BmrFI CCNGG 5 cut(s) 261, 277, 408, 616, 1158
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 6 cut(s) 236, 313, 391, 669, 835, 963
BmuI ACTGGG 1 cut(s) 349
BpiI GAAGAC 1 cut(s) 744
Bpu14I TTCGAA 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 277
BsaAI YACGTR 1 cut(s) 105
BsaBI GATNNNNATC 1 cut(s) 581
BsaHI GRCGYC 1 cut(s) 1056
BsaJI CCNNGG 5 cut(s) 259, 406, 490, 500, 1156
BsaWI WCCGGW 1 cut(s) 755
BsaXI ACNNNNNCTCC 6 cut(s) 341, 371, 592, 622, 737, 767
Bsc4I CCNNNNNNNGG 5 cut(s) 319, 386, 957, 1079, 1193
Bse1I ACTGG 4 cut(s) 220, 355, 469, 677
Bse21I CCTNAGG 1 cut(s) 1350
Bse3DI GCAATG 3 cut(s) 114, 768, 1291
Bse8I GATNNNNATC 1 cut(s) 581
BseBI CCWGG 4 cut(s) 261, 408, 616, 1158
BseDI CCNNGG 5 cut(s) 259, 406, 490, 500, 1156
BseJI GATNNNNATC 1 cut(s) 581
BseLI CCNNNNNNNGG 5 cut(s) 319, 386, 957, 1079, 1193
BseMI GCAATG 3 cut(s) 114, 768, 1291
BseMII CTCAG 2 cut(s) 356, 1341
BseNI ACTGG 4 cut(s) 220, 355, 469, 677
BseRI GAGGAG 1 cut(s) 362
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 2 cut(s) 187, 256
BseYI CCCAGC 1 cut(s) 786
BsgI GTGCAG 1 cut(s) 1243
BshFI GGCC 3 cut(s) 191, 361, 489
BshNI GGYRCC 2 cut(s) 476, 1055
BsiHKAI GWGCWC 3 cut(s) 139, 499, 1028
BsiSI CCGG 3 cut(s) 124, 276, 756
BslFI GGGAC 2 cut(s) 337, 521
BslI CCNNNNNNNGG 5 cut(s) 319, 386, 957, 1079, 1193
BsmAI GTCTC 4 cut(s) 345, 431, 757, 1122
BsmBI CGTCTC 1 cut(s) 345
BsmFI GGGAC 2 cut(s) 337, 521
BsmI GAATGC 2 cut(s) 83, 819
BsnI GGCC 3 cut(s) 191, 361, 489
Bsp119I TTCGAA 1 cut(s) 516
Bsp1286I GDGCHC 4 cut(s) 139, 258, 499, 1028
Bsp143I GATC 4 cut(s) 576, 960, 1087, 1183
BspACI CCGC 3 cut(s) 111, 237, 371
BspANI GGCC 3 cut(s) 191, 361, 489
BspCNI CTCAG 2 cut(s) 355, 1342
BspLI GGNNCC 4 cut(s) 157, 478, 687, 1057
BspMAI CTGCAG 2 cut(s) 205, 1399
BspMI ACCTGC 1 cut(s) 857
BspPI GGATC 4 cut(s) 571, 968, 1082, 1178
BspT104I TTCGAA 1 cut(s) 516
BspT107I GGYRCC 2 cut(s) 476, 1055
BsrDI GCAATG 3 cut(s) 114, 768, 1291
BsrI ACTGG 4 cut(s) 220, 355, 469, 677
BssECI CCNNGG 5 cut(s) 259, 406, 490, 500, 1156
BssMI GATC 4 cut(s) 576, 960, 1087, 1183
BssNI GRCGYC 1 cut(s) 1056
BssT1I CCWWGG 2 cut(s) 490, 500
Bst2UI CCWGG 4 cut(s) 261, 408, 616, 1158
Bst4CI ACNGT 5 cut(s) 134, 743, 874, 1064, 1164
Bst6I CTCTTC 2 cut(s) 369, 773
BstACI GRCGYC 1 cut(s) 1056
BstAPI GCANNNNNTGC 1 cut(s) 1403
BstBAI YACGTR 1 cut(s) 105
BstBI TTCGAA 1 cut(s) 516
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 2 cut(s) 342, 1350
BstENI CCTNNNNNAGG 1 cut(s) 317
BstH2I RGCGCY 1 cut(s) 1059
BstHHI GCGC 3 cut(s) 244, 1058, 1307
BstKTI GATC 4 cut(s) 579, 963, 1090, 1186
BstMAI GTCTC 4 cut(s) 345, 431, 757, 1122
BstMBI GATC 4 cut(s) 576, 960, 1087, 1183
BstMWI GCNNNNNNNGC 6 cut(s) 197, 253, 823, 875, 1321, 1403
BstNI CCWGG 4 cut(s) 261, 408, 616, 1158
BstSCI CCNGG 5 cut(s) 259, 275, 406, 614, 1156
BstSFI CTRYAG 3 cut(s) 201, 1123, 1395
BstSLI GKGCMC 1 cut(s) 258
BstSNI TACGTA 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 187, 256
BstV2I GAAGAC 1 cut(s) 744
BstX2I RGATCY 1 cut(s) 576
BstXI CCANNNNNNTGG 1 cut(s) 267
BstYI RGATCY 1 cut(s) 576
Bsu36I CCTNAGG 1 cut(s) 1350
BsuRI GGCC 3 cut(s) 191, 361, 489
BtsI GCAGTG 3 cut(s) 210, 374, 780
BtsIMutI CAGTG 6 cut(s) 139, 210, 362, 374, 780, 1260
BveI ACCTGC 1 cut(s) 857
Cac8I GCNNGC 1 cut(s) 121
CaiI CAGNNNCTG 1 cut(s) 1403
CfoI GCGC 3 cut(s) 244, 1058, 1307
CseI GACGC 1 cut(s) 327
Csp6I GTAC 3 cut(s) 106, 1007, 1036
CviAII CATG 1 cut(s) 717
CviQI GTAC 3 cut(s) 106, 1007, 1036
DdeI CTNAG 2 cut(s) 342, 1350
DinI GGCGCC 1 cut(s) 1057
DpnI GATC 4 cut(s) 578, 962, 1089, 1185
DpnII GATC 4 cut(s) 576, 960, 1087, 1183
EaeI YGGCCR 1 cut(s) 487
Eam1104I CTCTTC 2 cut(s) 369, 773
EarI CTCTTC 2 cut(s) 369, 773
EciI GGCGGA 3 cut(s) 100, 252, 360
Ecl136II GAGCTC 1 cut(s) 497
Eco105I TACGTA 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 490, 500
Eco147I AGGCCT 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 499
Eco53kI GAGCTC 1 cut(s) 497
Eco81I CCTNAGG 1 cut(s) 1350
EcoICRI GAGCTC 1 cut(s) 497
EcoNI CCTNNNNNAGG 1 cut(s) 317
EcoRI GAATTC 1 cut(s) 903
EcoRII CCWGG 4 cut(s) 259, 406, 614, 1156
EcoT14I CCWWGG 2 cut(s) 490, 500
EcoT22I ATGCAT 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 499
EgeI GGCGCC 1 cut(s) 1057
EheI GGCGCC 1 cut(s) 1057
ErhI CCWWGG 2 cut(s) 490, 500
Esp3I CGTCTC 1 cut(s) 345
FaeI CATG 1 cut(s) 720
FaqI GGGAC 2 cut(s) 337, 521
FatI CATG 1 cut(s) 716
Fnu4HI GCNGC 3 cut(s) 201, 245, 1398
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 3 cut(s) 201, 245, 1398
FspBI CTAG 2 cut(s) 335, 881
GlaI GCGC 3 cut(s) 243, 1057, 1306
GluI GCNGC 3 cut(s) 201, 245, 1398
GsaI CCCAGC 1 cut(s) 790
HaeII RGCGCY 1 cut(s) 1059
HaeIII GGCC 3 cut(s) 191, 361, 489
HapII CCGG 3 cut(s) 124, 276, 756
HgaI GACGC 1 cut(s) 327
HhaI GCGC 3 cut(s) 244, 1058, 1307
Hin1I GRCGYC 1 cut(s) 1056
Hin1II CATG 1 cut(s) 720
Hin6I GCGC 3 cut(s) 242, 1056, 1305
HinP1I GCGC 3 cut(s) 242, 1056, 1305
HinfI GANTC 7 cut(s) 88, 350, 713, 801, 947, 1197, 1291
HpaII CCGG 3 cut(s) 124, 276, 756
HphI GGTGA 6 cut(s) 176, 283, 529, 770, 849, 1145
Hpy166II GTNNAC 2 cut(s) 634, 1067
Hpy188I TCNGA 4 cut(s) 28, 232, 437, 576
Hpy188III TCNNGA 6 cut(s) 92, 344, 838, 908, 958, 1045
Hpy8I GTNNAC 2 cut(s) 634, 1067
HpyAV CCTTC 4 cut(s) 202, 323, 390, 408
HpyCH4III ACNGT 5 cut(s) 134, 743, 874, 1064, 1164
HpyCH4IV ACGT 3 cut(s) 104, 1019, 1038
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 253, 823, 875, 1321, 1403
HpyF3I CTNAG 2 cut(s) 342, 1350
HpySE526I ACGT 3 cut(s) 104, 1019, 1038
Hsp92I GRCGYC 1 cut(s) 1056
Hsp92II CATG 1 cut(s) 720
HspAI GCGC 3 cut(s) 242, 1056, 1305
KasI GGCGCC 1 cut(s) 1055
Kzo9I GATC 4 cut(s) 576, 960, 1087, 1183
LmnI GCTCC 6 cut(s) 155, 239, 279, 494, 502, 823
Lsp1109I GCAGC 2 cut(s) 187, 256
LweI GCATC 6 cut(s) 236, 313, 391, 669, 835, 963
MaeI CTAG 2 cut(s) 335, 881
MaeII ACGT 3 cut(s) 104, 1019, 1038
MaeIII GTNAC 8 cut(s) 100, 467, 535, 641, 743, 928, 1147, 1288
MalI GATC 4 cut(s) 578, 962, 1089, 1185
MboI GATC 4 cut(s) 576, 960, 1087, 1183
MboII GAAGA 8 cut(s) 6, 356, 359, 424, 749, 790, 935, 1023
MflI RGATCY 1 cut(s) 576
MhlI GDGCHC 4 cut(s) 139, 258, 499, 1028
MlsI TGGCCA 1 cut(s) 489
MluNI TGGCCA 1 cut(s) 489
Mly113I GGCGCC 1 cut(s) 1056
MlyI GAGTC 3 cut(s) 359, 707, 1285
MmeI TCCRAC 1 cut(s) 1146
Mox20I TGGCCA 1 cut(s) 489
Mph1103I ATGCAT 1 cut(s) 229
MscI TGGCCA 1 cut(s) 489
MseI TTAA 5 cut(s) 215, 705, 939, 1335, 1408
MslI CAYNNNNRTG 2 cut(s) 321, 773
Msp20I TGGCCA 1 cut(s) 489
MspI CCGG 3 cut(s) 124, 276, 756
MspR9I CCNGG 5 cut(s) 261, 277, 408, 616, 1158
Mva1269I GAATGC 2 cut(s) 83, 819
MvaI CCWGG 4 cut(s) 261, 408, 616, 1158
MwoI GCNNNNNNNGC 6 cut(s) 197, 253, 823, 875, 1321, 1403
NarI GGCGCC 1 cut(s) 1056
NciI CCSGG 1 cut(s) 277
NdeII GATC 4 cut(s) 576, 960, 1087, 1183
NlaIII CATG 1 cut(s) 720
NlaIV GGNNCC 4 cut(s) 157, 478, 687, 1057
NmuCI GTSAC 4 cut(s) 535, 928, 1147, 1288
NsiI ATGCAT 1 cut(s) 229
NspV TTCGAA 1 cut(s) 516
PceI AGGCCT 1 cut(s) 191
PctI GAATGC 2 cut(s) 83, 819
PfeI GAWTC 4 cut(s) 88, 801, 947, 1197
PkrI GCNGC 3 cut(s) 202, 246, 1399
PleI GAGTC 3 cut(s) 358, 707, 1285
PluTI GGCGCC 1 cut(s) 1059
PpsI GAGTC 3 cut(s) 358, 707, 1285
Ppu21I YACGTR 1 cut(s) 105
PshBI ATTAAT 1 cut(s) 1335
Psp124BI GAGCTC 1 cut(s) 499
Psp6I CCWGG 4 cut(s) 259, 406, 614, 1156
PspFI CCCAGC 1 cut(s) 786
PspGI CCWGG 4 cut(s) 259, 406, 614, 1156
PspN4I GGNNCC 4 cut(s) 157, 478, 687, 1057
PsrI GAACNNNNNNTAC 2 cut(s) 430, 462
PstI CTGCAG 2 cut(s) 205, 1399
PstNI CAGNNNCTG 1 cut(s) 1403
PsuI RGATCY 1 cut(s) 576
RsaI GTAC 3 cut(s) 107, 1008, 1037
RsaNI GTAC 3 cut(s) 106, 1007, 1036
RseI CAYNNNNRTG 2 cut(s) 321, 773
SacI GAGCTC 1 cut(s) 499
SaqAI TTAA 5 cut(s) 215, 705, 939, 1335, 1408
SatI GCNGC 3 cut(s) 201, 245, 1398
Sau3AI GATC 4 cut(s) 576, 960, 1087, 1183
SchI GAGTC 3 cut(s) 359, 707, 1285
ScrFI CCNGG 5 cut(s) 261, 277, 408, 616, 1158
SduI GDGCHC 4 cut(s) 139, 258, 499, 1028
SfaNI GCATC 6 cut(s) 236, 313, 391, 669, 835, 963
SfcI CTRYAG 3 cut(s) 201, 1123, 1395
SfoI GGCGCC 1 cut(s) 1057
SfuI TTCGAA 1 cut(s) 516
SmiMI CAYNNNNRTG 2 cut(s) 321, 773
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
SnaBI TACGTA 1 cut(s) 105
SseBI AGGCCT 1 cut(s) 191
SsiI CCGC 3 cut(s) 111, 237, 371
SspDI GGCGCC 1 cut(s) 1055
SspI AATATT 1 cut(s) 177
SspMI CTAG 2 cut(s) 335, 881
SstI GAGCTC 1 cut(s) 499
StuI AGGCCT 1 cut(s) 191
StyD4I CCNGG 5 cut(s) 259, 275, 406, 614, 1156
StyI CCWWGG 2 cut(s) 490, 500
TaaI ACNGT 5 cut(s) 134, 743, 874, 1064, 1164
TaiI ACGT 3 cut(s) 107, 1022, 1041
TaqI TCGA 3 cut(s) 516, 730, 1269
TaqII GACCGA 1 cut(s) 161
TfiI GAWTC 4 cut(s) 88, 801, 947, 1197
Tru1I TTAA 5 cut(s) 215, 705, 939, 1335, 1408
Tru9I TTAA 5 cut(s) 215, 705, 939, 1335, 1408
TscAI CASTG 6 cut(s) 139, 210, 362, 381, 780, 1267
TseFI GTSAC 4 cut(s) 535, 928, 1147, 1288
TseI GCWGC 3 cut(s) 200, 244, 1397
Tsp45I GTSAC 4 cut(s) 535, 928, 1147, 1288
TspDTI ATGAA 3 cut(s) 305, 372, 1046
TspGWI ACGGA 2 cut(s) 710, 813
TspRI CASTG 6 cut(s) 139, 210, 362, 381, 780, 1267
VspI ATTAAT 1 cut(s) 1335
XagI CCTNNNNNAGG 1 cut(s) 317
XapI RAATTY 2 cut(s) 806, 903
XspI CTAG 2 cut(s) 335, 881
Zsp2I ATGCAT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.