RchiOBHm_Chr4g0414931

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002711
Physical Location & Seq
Forward (+)
39446660 .. 39447457
798 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38526

Sequence Viewer

Length: 798 bp
ATGCCACTGTGGGATAACATTCTGGTATTAATTTGCAGTAACATTACTATACAAGGCCTCACTATCCTTGCACCAGTTAGCGTTCCTAACACAGATGGGATTAACCCCGATTCGTGTTCACAAACTCGAATTGAAGACTGTTACATAGTCTCCGGGGATGACTGCATTGCAGTGAAGAGTGGCTGGGATCAGTATGGAATCAAATTTGGAATGCCAACCGAGCATCTTGTTATCAGGAGGCTTACCTGTATTTCACCTAAAAGTGCTACTGTTGCCCTAGGCAGTGAAATGTCTGGTGGAATCAGGGATGTAAGAGTTGAAGATGTCACAGCCATTCACACCGAATCCAGCATTAGGATCAAAACAGCTGTAGGCAGAGGGGCTTATGTCAAAGACATTTATGTACGAAGATTGACGTTGAAGACTATGAAGTACGTTTTTTGGATGACAGGGGGCTATGGTTCACATCCAGGCGAGGGCTTTGATCCAAAGGCACTTCCTGCGATTCAAAACATCAACTATAGAGAGGTTGTTGCTGAGAATGTTACAATGCCAGCCCAGTTGGATGGAATTGCTAATGATCCTTTCAAGGGAATTTGCATCTCTAATGTGAGTATCTCACTCACTGAAAAGCCAAAGAAATTGTTGTGGAACTGTACTGATGTTGAGGGCATTACGAGCAATGTGACTCCAAAGGCTTGCGATTTGTTGCCCGAGCAGCAAAAACCAATCGATTGTGCTTTCCCTGAGGATAAGTTAGCCATTGAGGGTGTTAAGCTGACCACTTGTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

265

Amino Acids

28.87

Weight (kDa)

5.48

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 10 - 223 2.5e-22 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 195, 365, 479, 575
AcsI RAATTY 2 cut(s) 203, 594
AfaI GTAC 3 cut(s) 405, 434, 658
AfiI CCNNNNNNNGG 3 cut(s) 354, 476, 590
AgsI TTSAA 5 cut(s) 134, 320, 421, 509, 589
AjnI CCWGG 1 cut(s) 469
AluBI AGCT 2 cut(s) 368, 778
AluI AGCT 2 cut(s) 368, 778
Alw26I GTCTC 1 cut(s) 154
AlwI GGATC 4 cut(s) 195, 365, 479, 575
Ama87I CYCGRG 1 cut(s) 713
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 718
ApoI RAATTY 2 cut(s) 203, 594
AseI ATTAAT 1 cut(s) 29
AspA2I CCTAGG 1 cut(s) 277
AsuC2I CCSGG 1 cut(s) 154
AsuHPI GGTGA 1 cut(s) 246
AvaI CYCGRG 1 cut(s) 713
AvrII CCTAGG 1 cut(s) 277
AxyI CCTNAGG 1 cut(s) 747
BbsI GAAGAC 2 cut(s) 141, 428
BbvI GCAGC 1 cut(s) 730
BccI CCATC 2 cut(s) 89, 560
BciT130I CCWGG 1 cut(s) 471
BcnI CCSGG 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 154
BfaI CTAG 1 cut(s) 278
BfmI CTRYAG 2 cut(s) 369, 520
BisI GCNGC 1 cut(s) 719
BlnI CCTAGG 1 cut(s) 277
BlsI GCNGC 1 cut(s) 720
Bme1390I CCNGG 2 cut(s) 154, 471
BmeT110I CYCGRG 1 cut(s) 713
BmrFI CCNGG 2 cut(s) 154, 471
BmrI ACTGGG 1 cut(s) 553
BmsI GCATC 2 cut(s) 232, 609
BmuI ACTGGG 1 cut(s) 553
BpiI GAAGAC 2 cut(s) 141, 428
BpuMI CCSGG 1 cut(s) 154
Bsa29I ATCGAT 1 cut(s) 732
BsaJI CCNNGG 2 cut(s) 153, 277
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 3 cut(s) 354, 476, 590
Bse1I ACTGG 2 cut(s) 74, 559
Bse21I CCTNAGG 1 cut(s) 747
Bse3DI GCAATG 2 cut(s) 165, 688
BseBI CCWGG 1 cut(s) 471
BseCI ATCGAT 1 cut(s) 732
BseDI CCNNGG 2 cut(s) 153, 277
BseGI GGATG 5 cut(s) 163, 313, 450, 466, 571
BseLI CCNNNNNNNGG 3 cut(s) 354, 476, 590
BseMI GCAATG 2 cut(s) 165, 688
BseMII CTCAG 2 cut(s) 528, 738
BseNI ACTGG 2 cut(s) 74, 559
BseXI GCAGC 1 cut(s) 730
BseYI CCCAGC 1 cut(s) 183
BshFI GGCC 1 cut(s) 57
BshVI ATCGAT 1 cut(s) 732
BsiHKCI CYCGRG 1 cut(s) 713
BsiSI CCGG 1 cut(s) 153
BslI CCNNNNNNNGG 3 cut(s) 354, 476, 590
BsmAI GTCTC 1 cut(s) 154
BsmI GAATGC 1 cut(s) 216
BsnI GGCC 1 cut(s) 57
BsoBI CYCGRG 1 cut(s) 713
Bsp143I GATC 4 cut(s) 187, 357, 484, 580
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 2 cut(s) 529, 739
BspDI ATCGAT 1 cut(s) 732
BspPI GGATC 4 cut(s) 195, 365, 479, 575
BsrDI GCAATG 2 cut(s) 165, 688
BsrI ACTGG 2 cut(s) 74, 559
BssECI CCNNGG 2 cut(s) 153, 277
BssMI GATC 4 cut(s) 187, 357, 484, 580
BssT1I CCWWGG 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 4 cut(s) 9, 140, 271, 656
Bst6I CTCTTC 1 cut(s) 170
BstAPI GCANNNNNTGC 1 cut(s) 500
BstC8I GCNNGC 2 cut(s) 555, 700
BstDEI CTNAG 2 cut(s) 537, 747
BstF5I GGATG 5 cut(s) 163, 313, 450, 466, 571
BstKTI GATC 4 cut(s) 190, 360, 487, 583
BstMAI GTCTC 1 cut(s) 154
BstMBI GATC 4 cut(s) 187, 357, 484, 580
BstMWI GCNNNNNNNGC 5 cut(s) 220, 272, 500, 678, 718
BstNI CCWGG 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 152, 469
BstSFI CTRYAG 2 cut(s) 369, 520
BstV1I GCAGC 1 cut(s) 730
BstV2I GAAGAC 2 cut(s) 141, 428
BstXI CCANNNNNNTGG 1 cut(s) 566
Bsu15I ATCGAT 1 cut(s) 732
Bsu36I CCTNAGG 1 cut(s) 747
BsuRI GGCC 1 cut(s) 57
BsuTUI ATCGAT 1 cut(s) 732
BtsCI GGATG 5 cut(s) 163, 313, 450, 466, 571
BtsI GCAGTG 2 cut(s) 177, 289
BtsIMutI CAGTG 4 cut(s) 5, 177, 289, 624
Cac8I GCNNGC 2 cut(s) 555, 700
ClaI ATCGAT 1 cut(s) 732
Csp6I GTAC 3 cut(s) 404, 433, 657
CviQI GTAC 3 cut(s) 404, 433, 657
DdeI CTNAG 2 cut(s) 537, 747
DpnI GATC 4 cut(s) 189, 359, 486, 582
DpnII GATC 4 cut(s) 187, 357, 484, 580
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 277
Eco147I AGGCCT 1 cut(s) 57
Eco81I CCTNAGG 1 cut(s) 747
Eco88I CYCGRG 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 277
ErhI CCWWGG 1 cut(s) 277
FaiI YATR 8 cut(s) 50, 146, 195, 387, 402, 428, 459, 522
Fnu4HI GCNGC 1 cut(s) 719
FokI GGATG 5 cut(s) 170, 320, 453, 457, 578
Fsp4HI GCNGC 1 cut(s) 719
FspBI CTAG 1 cut(s) 278
GluI GCNGC 1 cut(s) 719
GsaI CCCAGC 1 cut(s) 187
HaeIII GGCC 1 cut(s) 57
HapII CCGG 1 cut(s) 153
HinfI GANTC 6 cut(s) 110, 198, 300, 344, 505, 688
HpaII CCGG 1 cut(s) 153
HphI GGTGA 1 cut(s) 246
Hpy166II GTNNAC 2 cut(s) 119, 464
Hpy188III TCNNGA 1 cut(s) 235
Hpy8I GTNNAC 2 cut(s) 119, 464
HpyCH4III ACNGT 4 cut(s) 9, 140, 271, 656
HpyCH4IV ACGT 2 cut(s) 416, 435
HpyCH4V TGCA 5 cut(s) 36, 71, 165, 170, 600
HpyF10VI GCNNNNNNNGC 5 cut(s) 220, 272, 500, 678, 718
HpyF3I CTNAG 2 cut(s) 537, 747
HpySE526I ACGT 2 cut(s) 416, 435
Kzo9I GATC 4 cut(s) 187, 357, 484, 580
Lsp1109I GCAGC 1 cut(s) 730
LweI GCATC 2 cut(s) 232, 609
MaeI CTAG 1 cut(s) 278
MaeII ACGT 2 cut(s) 416, 435
MaeIII GTNAC 5 cut(s) 38, 140, 325, 544, 685
MalI GATC 4 cut(s) 189, 359, 486, 582
MboI GATC 4 cut(s) 187, 357, 484, 580
MboII GAAGA 5 cut(s) 146, 187, 332, 420, 433
MluCI AATT 6 cut(s) 30, 129, 203, 570, 594, 641
MlyI GAGTC 1 cut(s) 682
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 8 cut(s) 68, 231, 371, 469, 520, 661, 742, 760
MseI TTAA 4 cut(s) 29, 102, 774, 796
MslI CAYNNNNRTG 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 2 cut(s) 154, 471
Mva1269I GAATGC 1 cut(s) 216
MvaI CCWGG 1 cut(s) 471
MwoI GCNNNNNNNGC 5 cut(s) 220, 272, 500, 678, 718
NciI CCSGG 1 cut(s) 154
NdeII GATC 4 cut(s) 187, 357, 484, 580
NmuCI GTSAC 2 cut(s) 325, 685
PceI AGGCCT 1 cut(s) 57
PctI GAATGC 1 cut(s) 216
PfeI GAWTC 5 cut(s) 110, 198, 300, 344, 505
PkrI GCNGC 1 cut(s) 720
PleI GAGTC 1 cut(s) 682
PpsI GAGTC 1 cut(s) 682
PshBI ATTAAT 1 cut(s) 29
Psp6I CCWGG 1 cut(s) 469
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 1 cut(s) 469
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 3 cut(s) 405, 434, 658
RsaNI GTAC 3 cut(s) 404, 433, 657
RseI CAYNNNNRTG 1 cut(s) 170
SaqAI TTAA 4 cut(s) 29, 102, 774, 796
SatI GCNGC 1 cut(s) 719
Sau3AI GATC 4 cut(s) 187, 357, 484, 580
SchI GAGTC 1 cut(s) 682
ScrFI CCNGG 2 cut(s) 154, 471
SetI ASST 7 cut(s) 248, 259, 370, 419, 438, 531, 780
SfaNI GCATC 2 cut(s) 232, 609
SfcI CTRYAG 2 cut(s) 369, 520
SmiMI CAYNNNNRTG 1 cut(s) 170
Sse9I AATT 6 cut(s) 30, 129, 203, 570, 594, 641
SseBI AGGCCT 1 cut(s) 57
SspMI CTAG 1 cut(s) 278
StuI AGGCCT 1 cut(s) 57
StyD4I CCNGG 2 cut(s) 152, 469
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 4 cut(s) 9, 140, 271, 656
TaiI ACGT 2 cut(s) 419, 438
TaqI TCGA 2 cut(s) 127, 732
TasI AATT 6 cut(s) 30, 129, 203, 570, 594, 641
TatI WGTACW 1 cut(s) 656
TfiI GAWTC 5 cut(s) 110, 198, 300, 344, 505
Tru1I TTAA 4 cut(s) 29, 102, 774, 796
Tru9I TTAA 4 cut(s) 29, 102, 774, 796
TscAI CASTG 4 cut(s) 12, 177, 289, 631
TseFI GTSAC 2 cut(s) 325, 685
TseI GCWGC 1 cut(s) 718
Tsp45I GTSAC 2 cut(s) 325, 685
TspDTI ATGAA 1 cut(s) 443
TspRI CASTG 4 cut(s) 12, 177, 289, 631
VspI ATTAAT 1 cut(s) 29
XapI RAATTY 2 cut(s) 203, 594
XmaJI CCTAGG 1 cut(s) 277
XspI CTAG 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.