Rh1AG429700

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0002711
Physical Location & Seq
Forward (+)
65678905 .. 65679507
603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG429700.1

Sequence Viewer

Length: 603 bp
ATGCCGACAGAGCATGTTGTGATCAGGAGGCTCACCTGCATTTCACCTAAAAGTGCCACCATTGCCCTAGGCAGTGAAATGTCCGGAGGAATCAGAGACGTTAGAGTTGAGGACGTCACAGCTATTAACACTGAATCCAGCATTAGGATCAAAACCGCTGTTGGAAGAGGGGCTTATGTCAAAGATATCTATGCAAGAAGAGTGACCTTGAAGACAATGAAATATGTTTTTTGGATGACAGGCGCTTATGGTTCGCATCCGAACAATGATTTTGATCCAAATGCACTTCCTGTGATTCAAAACATCAACTTTAAAGAGTTCGTGGCTGAGAATGTTTCCATTCCAGCACAATTAGATGGAATTGCTAATGACCCTTTCAAGGGAATTTGCATGTCTAATGTTTCTATCTCACTTTCTGCGAATCCTACGAAACTTCTGTGGAATTGTACTGGTATTGAGGGAATTACGAGCAATGTGACTCCGAAGGCATGTGATTTGTTACTCCAGCAGCAAAAACCAGTCGATTGTGCTTTCCCCGAGGATAAGTTAGCCATTGAAGGGTTTAACTTGACGACTTGTTTTGCAGGTTTTGCCAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

200

Amino Acids

21.79

Weight (kDa)

7.57

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 23 - 153 1.5e-08 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 44
AatII GACGTC 1 cut(s) 117
Acc36I ACCTGC 2 cut(s) 44, 575
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 1 cut(s) 156
AclWI GGATC 2 cut(s) 155, 269
AcsI RAATTY 1 cut(s) 384
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 1 cut(s) 448
AfiI CCNNNNNNNGG 4 cut(s) 144, 379, 380, 558
AgsI TTSAA 4 cut(s) 211, 299, 379, 557
AluBI AGCT 1 cut(s) 122
AluI AGCT 1 cut(s) 122
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 2 cut(s) 155, 269
Ama87I CYCGRG 1 cut(s) 536
Aor13HI TCCGGA 1 cut(s) 83
ApeKI GCWGC 1 cut(s) 508
ApoI RAATTY 1 cut(s) 384
AspA2I CCTAGG 1 cut(s) 67
AspLEI GCGC 1 cut(s) 245
AsuHPI GGTGA 2 cut(s) 25, 36
AvaI CYCGRG 1 cut(s) 536
AvrII CCTAGG 1 cut(s) 67
BbsI GAAGAC 1 cut(s) 218
BbvI GCAGC 1 cut(s) 520
BccI CCATC 1 cut(s) 350
BclI TGATCA 1 cut(s) 21
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 1 cut(s) 68
BfoI RGCGCY 1 cut(s) 246
BfuAI ACCTGC 2 cut(s) 44, 575
BisI GCNGC 1 cut(s) 509
BlnI CCTAGG 1 cut(s) 67
BlsI GCNGC 1 cut(s) 510
BmeT110I CYCGRG 1 cut(s) 536
BmsI GCATC 1 cut(s) 265
BpiI GAAGAC 1 cut(s) 218
BpmI CTGGAG 1 cut(s) 488
BsaBI GATNNNNATC 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 3 cut(s) 67, 537, 594
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 379, 380, 558
Bse1I ACTGG 2 cut(s) 454, 518
Bse3DI GCAATG 2 cut(s) 60, 478
Bse8I GATNNNNATC 1 cut(s) 273
BseAI TCCGGA 1 cut(s) 83
BseDI CCNNGG 3 cut(s) 67, 537, 594
BseGI GGATG 2 cut(s) 240, 256
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 4 cut(s) 144, 379, 380, 558
BseMI GCAATG 2 cut(s) 60, 478
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 2 cut(s) 454, 518
BseXI GCAGC 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 1 cut(s) 84
BslI CCNNNNNNNGG 4 cut(s) 144, 379, 380, 558
BsmAI GTCTC 1 cut(s) 90
BsmBI CGTCTC 1 cut(s) 90
BsoBI CYCGRG 1 cut(s) 536
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 3 cut(s) 21, 147, 274
BspACI CCGC 1 cut(s) 156
BspCNI CTCAG 1 cut(s) 319
BspEI TCCGGA 1 cut(s) 83
BspMI ACCTGC 2 cut(s) 44, 575
BspPI GGATC 2 cut(s) 155, 269
BsrDI GCAATG 2 cut(s) 60, 478
BsrI ACTGG 2 cut(s) 454, 518
BssECI CCNNGG 3 cut(s) 67, 537, 594
BssMI GATC 3 cut(s) 21, 147, 274
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 67, 594
Bst6I CTCTTC 2 cut(s) 160, 193
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 590
BstDEI CTNAG 1 cut(s) 327
BstF5I GGATG 2 cut(s) 240, 256
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 3 cut(s) 24, 150, 277
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 3 cut(s) 21, 147, 274
BstMWI GCNNNNNNNGC 3 cut(s) 10, 62, 590
BstNSI RCATGY 3 cut(s) 17, 394, 492
BstV1I GCAGC 1 cut(s) 520
BstV2I GAAGAC 1 cut(s) 218
BtsCI GGATG 2 cut(s) 240, 256
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 79, 129
BveI ACCTGC 2 cut(s) 44, 575
CfoI GCGC 1 cut(s) 245
Csp6I GTAC 1 cut(s) 447
CviAII CATG 3 cut(s) 14, 391, 489
CviJI RGCY 5 cut(s) 31, 122, 173, 326, 551
CviKI_1 RGCY 5 cut(s) 31, 122, 173, 326, 551
CviQI GTAC 1 cut(s) 447
DdeI CTNAG 1 cut(s) 327
DpnI GATC 3 cut(s) 23, 149, 276
DpnII GATC 3 cut(s) 21, 147, 274
DraI TTTAAA 1 cut(s) 313
Eam1104I CTCTTC 2 cut(s) 160, 193
EarI CTCTTC 2 cut(s) 160, 193
Eco130I CCWWGG 2 cut(s) 67, 594
Eco32I GATATC 1 cut(s) 187
Eco88I CYCGRG 1 cut(s) 536
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 2 cut(s) 67, 594
ErhI CCWWGG 2 cut(s) 67, 594
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 3 cut(s) 17, 394, 492
FaiI YATR 7 cut(s) 15, 177, 192, 225, 249, 392, 490
FalI AAGNNNNNCTT 2 cut(s) 157, 189
FatI CATG 3 cut(s) 13, 390, 488
FbaI TGATCA 1 cut(s) 21
Fnu4HI GCNGC 1 cut(s) 509
FokI GGATG 2 cut(s) 243, 247
Fsp4HI GCNGC 1 cut(s) 509
FspBI CTAG 1 cut(s) 68
GlaI GCGC 1 cut(s) 244
GluI GCNGC 1 cut(s) 509
GsuI CTGGAG 1 cut(s) 488
HaeII RGCGCY 1 cut(s) 246
HapII CCGG 1 cut(s) 84
HhaI GCGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 3 cut(s) 17, 394, 492
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HinfI GANTC 5 cut(s) 90, 134, 295, 421, 478
HpaII CCGG 1 cut(s) 84
HphI GGTGA 2 cut(s) 25, 36
Hpy188I TCNGA 3 cut(s) 95, 261, 483
Hpy188III TCNNGA 2 cut(s) 25, 84
HpyAV CCTTC 2 cut(s) 478, 551
HpyCH4IV ACGT 2 cut(s) 99, 114
HpyCH4V TGCA 5 cut(s) 39, 194, 284, 390, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 62, 590
HpyF3I CTNAG 1 cut(s) 327
HpySE526I ACGT 2 cut(s) 99, 114
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 3 cut(s) 17, 394, 492
HspAI GCGC 1 cut(s) 243
Kpn2I TCCGGA 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 21
Kzo9I GATC 3 cut(s) 21, 147, 274
Lsp1109I GCAGC 1 cut(s) 520
LweI GCATC 1 cut(s) 265
MaeI CTAG 1 cut(s) 68
MaeII ACGT 2 cut(s) 99, 114
MaeIII GTNAC 4 cut(s) 115, 202, 475, 498
MalI GATC 3 cut(s) 23, 149, 276
MboI GATC 3 cut(s) 21, 147, 274
MboII GAAGA 3 cut(s) 177, 210, 223
MluCI AATT 5 cut(s) 350, 360, 384, 442, 462
MlyI GAGTC 1 cut(s) 472
MmeI TCCRAC 1 cut(s) 142
MnlI CCTC 6 cut(s) 21, 80, 103, 161, 451, 532
MroI TCCGGA 1 cut(s) 83
MseI TTAA 3 cut(s) 126, 312, 564
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 1 cut(s) 84
MwoI GCNNNNNNNGC 3 cut(s) 10, 62, 590
NdeII GATC 3 cut(s) 21, 147, 274
NlaIII CATG 3 cut(s) 17, 394, 492
NmuCI GTSAC 3 cut(s) 115, 202, 475
NspI RCATGY 3 cut(s) 17, 394, 492
PaqCI CACCTGC 1 cut(s) 44
PfeI GAWTC 4 cut(s) 90, 134, 295, 421
PkrI GCNGC 1 cut(s) 510
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
RsaI GTAC 1 cut(s) 448
RsaNI GTAC 1 cut(s) 447
SaqAI TTAA 3 cut(s) 126, 312, 564
SatI GCNGC 1 cut(s) 509
Sau3AI GATC 3 cut(s) 21, 147, 274
SchI GAGTC 1 cut(s) 472
SetI ASST 8 cut(s) 38, 49, 102, 117, 124, 209, 589, 600
SfaNI GCATC 1 cut(s) 265
Sse9I AATT 5 cut(s) 350, 360, 384, 442, 462
SsiI CCGC 1 cut(s) 156
SspMI CTAG 1 cut(s) 68
StyI CCWWGG 2 cut(s) 67, 594
TaiI ACGT 2 cut(s) 102, 117
TaqI TCGA 1 cut(s) 522
TasI AATT 5 cut(s) 350, 360, 384, 442, 462
TatI WGTACW 1 cut(s) 446
TfiI GAWTC 4 cut(s) 90, 134, 295, 421
Tru1I TTAA 3 cut(s) 126, 312, 564
Tru9I TTAA 3 cut(s) 126, 312, 564
TscAI CASTG 2 cut(s) 79, 136
TseFI GTSAC 3 cut(s) 115, 202, 475
TseI GCWGC 1 cut(s) 508
Tsp45I GTSAC 3 cut(s) 115, 202, 475
TspDTI ATGAA 1 cut(s) 233
TspRI CASTG 2 cut(s) 79, 136
XapI RAATTY 1 cut(s) 384
XceI RCATGY 3 cut(s) 17, 394, 492
XmaJI CCTAGG 1 cut(s) 67
XspI CTAG 1 cut(s) 68
ZraI GACGTC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.